This course syllabus outlines the topics, schedule, and requirements for a graduate level Molecular and Cellular Bioengineering course. Over 50 contact hours, the course will cover topics such as DNA engineering, gene regulation, molecular and cellular techniques, cellular functions, cell-environment interactions, tissue engineering, and advanced topics including stem cell biology and fluorescence proteins/biosensors. Students will be evaluated based on five quizzes, a class presentation, and a final exam. Recommended textbooks and research papers are provided as additional references for each major topic.
Radiation Response of Bacteria Associated with Human Cancellous BoneIOSR Journals
Cancellous bones from twenty five live tissue donors were tested for bacterial contamination and initial bioburden ranged from 4.1×101 to 3.1×103 cfu/g (average 9.0×102 cfu/g). Forty six representative bacterial isolates were characterized on the basis of morphological, cultural and biochemical characteristics. Staphylococcus spp. was found to be predominant contaminant in tissue samples (41.30%). To assess the radiation resistance all the bacterial isolates were exposed to 1 to 10 kGy gamma radiation from 60Co gamma source. The radiation decimal reduction dose values (D10) and twelve log reduction values (12 D value) of the isolates were calculated. D10 values of the isolates were ranged from 0.59 to 1.20 kGy. Among the studied bacterial isolates, Streptococcus spp. was the most radioresistant isolates (D10 value 0.93-1.20 kGy) and three of the Streptococcus spp. survived up to 8 kGy. All the bacterial isolates were killed at 9 kGy. Twelve log reduction value (12D value) of the most resistant isolate was 14.4 kGy. These results indicate that standard radiation sterilization dose (25 kGy) is satisfactory for the sterilization of the cancellous bone allografts
Radiation Response of Bacteria Associated with Human Cancellous BoneIOSR Journals
Cancellous bones from twenty five live tissue donors were tested for bacterial contamination and initial bioburden ranged from 4.1×101 to 3.1×103 cfu/g (average 9.0×102 cfu/g). Forty six representative bacterial isolates were characterized on the basis of morphological, cultural and biochemical characteristics. Staphylococcus spp. was found to be predominant contaminant in tissue samples (41.30%). To assess the radiation resistance all the bacterial isolates were exposed to 1 to 10 kGy gamma radiation from 60Co gamma source. The radiation decimal reduction dose values (D10) and twelve log reduction values (12 D value) of the isolates were calculated. D10 values of the isolates were ranged from 0.59 to 1.20 kGy. Among the studied bacterial isolates, Streptococcus spp. was the most radioresistant isolates (D10 value 0.93-1.20 kGy) and three of the Streptococcus spp. survived up to 8 kGy. All the bacterial isolates were killed at 9 kGy. Twelve log reduction value (12D value) of the most resistant isolate was 14.4 kGy. These results indicate that standard radiation sterilization dose (25 kGy) is satisfactory for the sterilization of the cancellous bone allografts
Nanobiotechnological applications in dna therapySenthil Natesan
Gene therapy is a form of molecular medicine that has the potential to influence significantly human health in this 21st century. It promises to provide new treatments for a large number of inherited and acquired diseases (Verma and Weitzman, 2005). The basic concept of gene therapy is simple which includes introduction of a piece of genetic material into target cells that will result in either a cure for the disease or a slowdown in the progression of the disease. To achieve this goal, gene therapy requires technologies capable of gene transfer into a wide variety of cells, tissues, and organs. A key factor in the success of gene therapy is the development of delivery systems that are capable of efficient gene transfer in a variety of tissues, without causing any associated pathogenic effects. Vectors based upon many different viral systems, including retroviruses, lentiviruses, adenoviruses, and adeno-associated viruses, currently offer the best choice for efficient gene delivery.
Deb Newberry, Director of the Nano-Link program at Dakota County Technical College, MN, talks about the exciting area where nanotechnology and biotechnology converge.
As the needs of an increasing population demand new solutions, we believe the answers are growing. That’s why Life Technologies is committed to providing instruments, reagents, and technologies for Agriculture Biotechnology that will lead the way to remarkable agricultural discoveries—everything from improved crops that feed more people to sustainable biofuels that keep things moving.
For more info visit:
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/agricultural-biotechnology.html?cid=fl-agbio?CID=Agbiooverview-SS-12312
OMICS Publishing Group, Journal of Biochips & Tissue Chips functions as an inevitable resource for scientists in advancement of pharmaceutical and biotechnological research/industrial applications. The Journal of Biochips & Tissue Chips helps to share researchers to share their studies, research and innovations with the common platform for better awareness in this arena.
Nanobiotechnological applications in dna therapySenthil Natesan
Gene therapy is a form of molecular medicine that has the potential to influence significantly human health in this 21st century. It promises to provide new treatments for a large number of inherited and acquired diseases (Verma and Weitzman, 2005). The basic concept of gene therapy is simple which includes introduction of a piece of genetic material into target cells that will result in either a cure for the disease or a slowdown in the progression of the disease. To achieve this goal, gene therapy requires technologies capable of gene transfer into a wide variety of cells, tissues, and organs. A key factor in the success of gene therapy is the development of delivery systems that are capable of efficient gene transfer in a variety of tissues, without causing any associated pathogenic effects. Vectors based upon many different viral systems, including retroviruses, lentiviruses, adenoviruses, and adeno-associated viruses, currently offer the best choice for efficient gene delivery.
Deb Newberry, Director of the Nano-Link program at Dakota County Technical College, MN, talks about the exciting area where nanotechnology and biotechnology converge.
As the needs of an increasing population demand new solutions, we believe the answers are growing. That’s why Life Technologies is committed to providing instruments, reagents, and technologies for Agriculture Biotechnology that will lead the way to remarkable agricultural discoveries—everything from improved crops that feed more people to sustainable biofuels that keep things moving.
For more info visit:
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/agricultural-biotechnology.html?cid=fl-agbio?CID=Agbiooverview-SS-12312
OMICS Publishing Group, Journal of Biochips & Tissue Chips functions as an inevitable resource for scientists in advancement of pharmaceutical and biotechnological research/industrial applications. The Journal of Biochips & Tissue Chips helps to share researchers to share their studies, research and innovations with the common platform for better awareness in this arena.
What is the subject ?: To solve one of the youth’ problems in the west
……………………………….
Who are we ? Parents and imams
why ? To learn how to deal with youth
Where ? Islamic center in Sydney
When ? Today
How to follow the Prophet Mohammed (PBUH) and glorify his Sunnah
Alatba’a is a must
Six conditions for following the prophet’s acts of worship
The benefits of following Sunna
Symptoms of Following our prophet
Our third webinar in the MDC Connects Series 2021 | A Guide to Complex Medicines.
This slide deck gives an overview of the early assessment of Prototype Nanomedicine Nano Bio Interactions.
Zahra Rattray, University of Strathclyde
Introduction to Synthetic Genome
SYNTHETIC GENOMICS Study of Invitro chemical synthesis of genetic material i.e., DNA in the form of oligonucleotides, genes, or genomes with Computational techniques for its design. SYNTHETIC GENOME Artificially synthesised genome (invitro)
Shining a light on virus infection with high-resolution microscopyRoland Remenyi, PhD
Each year the Astbury Centre holds a research retreat to promote interdisciplinary activity, to discuss and present latest findings and to promote and encourage interdisciplinary research at the highest level. I communicated my results from a Chikungunya virus project in a 15-min presentation. Slides from this presentation are shown here.
1. Course Syllabus
Subject and Course Number: BIOE 506
Title: Molecular & Cellular Bioengrg
Proposed Texts: On Line Lecture Notes
Recommended Texts: Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K., Walter, P.:
Molecular Biology of the Cell. 4th ed., Garland Science, Taylor & Francis Group, 2002
Credit: 4 graduate hours.
Contact Hours: Two 90-minute lectures-discussions per week. 3.5 contact hours per week.
Topics Contact Hours
I. Introduction to Molecular and Cellular Engineering
• Introduction and review of DNA, RNA, Protein, Organelles, Cells 4
II. DNA Engineering and Technologies
• PCR, RT-PCR, DNA Mutagenesis, DNA Shuffling, Directed Evolution 2
• Northern Blot, Southern Blot, Microarray 2
III. Gene Regulation and Genetic Engineering
• Transcription, RNA, SiRNA, Gene Therapy 2
IV. Molecular Engineering and Technologies
• Antibodies, Electrophoresis, Immunoblotting, Immunostaining 2
• Kinase assay, Yeast Two-Hybrid Assay, Flow Cytometry 2
V. Cellular Functions
• Cytoskeleton 2
• Signaling Transduction, Phosphorylation, GTPases, Protein-Protein interaction 4
• Cell-Extracellular Matrix Adhesions 2
• Cell-Cell adhesions 2
VI. Cell-Environment Interactions
• Outside-in: Receptors, Cell Signaling and Cytoskeleton; Inside-out: Cell
Traction Force, Extracellular Matrix remodeling; The measurement of
intracellular strain using marker displacement, of external traction force using
beads-embedded elastic gels and nanofabricated elastic poles. The effects of
mechanical properties.
6
VII. Cellular and Tissue Engineering
• Biomaterials, Nanofabrication 2
• Cell-Material Interaction, Tissue Engineering, 3D Culture 2
• Signaling and Regulation 2
VIII. Advanced Topics: (I) Stem Cell Biology
• Maintenance, Differentiation, Proliferation 2
• Stem cells for tissue engineering 4
IX. Advanced Topics: (II) Fluorescence Proteins and Biosensors
2. • Fluorescence Proteins 3
• Genetically-encoded Biosensors based on Fluorescence Proteins.
The application of biosensors for measuring cell-environment interactions 2
Five quizzes, 40 minutes for each quiz 3
Total 50
Grading: Grade will be based on five quizzes (40%), class presentation (30%), and final exam (30%).
Proposed By: Dr. Yingxiao Wang
3. References Addendum
Topics Class Time References
X. Introduction to Molecular and Cellular Engineering
• Introduction and review of DNA, RNA, Protein, Organelles, Cells 4 hr (1)
XI. DNA Engineering and Technologies
• PCR, RT-PCR, DNA Mutagenesis, DNA Shuffling, Directed Evolution 2 hr (2, 3)
• Northern Blot, Southern Blot, Microarray 2 hr (1, 3, 4)
XII. Gene Regulation and Genetic Engineering
• Transcription, RNA, SiRNA, Gene Therapy 2 hr (1, 5)
XIII. Molecular Engineering and Technologies
• Antibodies, Electrophoresis, Immunoblotting, Immunostaining 2 hr (1, 3, 6)
• Kinase assay, Yeast Two-Hybrid Assay, Flow Cytometry 2 hr (3)
XIV. Cellular Functions
• Cytoskeleton 2 hr (1)
• Signaling Transduction, Phosphorylation, GTPases, Protein-Protein interaction
4 hr (7-9)
• Cell-Extracellular Matrix Adhesions 2 hr (10-13)
• Cell-Cell adhesions 2 hr (14-17)
XV. Cell-Environment Interactions
• Outside-in: Receptors, Cell Signaling and Cytoskeleton; Inside-out: Cell
Traction Force, Extracellular Matrix remodeling; The measurement of
intracellular strain using marker displacement, of external traction force using
beads-embedded elastic gels and nanofabricated elastic poles. The effects of
mechanical properties.
6 hr (1, 18-20)
XVI. Cellular and Tissue Engineering
• Biomaterials, Nanofabrication 2 hr (21, 22)
• Cell-Material Interaction, Tissue Engineering, 3D Culture 2 hr (23-25)
• Signaling and Regulation 2 hr (26, 27)
XVII. Advanced Topics: (I) Stem Cell Biology
• Maintenance, Differentiation, Proliferation 2 hr (1)
• Stem cells for tissue engineering 4 hr (28-32)
XVIII. Advanced Topics: (II) Fluorescence Proteins and Biosensors
• Fluorescence Proteins 3 hr (33-35)
• Genetically-encoded Biosensors based on Fluorescence Proteins,
The application of biosensors for measuring cell-environment interactions 2 hr (36-38)
Five quizzes, 40 minutes for each quiz 3 hr
Total 50 hr
4. Reference:
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Science, Taylor & Francis Group
2. James D. Watson MG, Jan A. Witkowski, Mark Zoller 1992. W. H. Freeman
3. Joseph Sambrook DWR. 2001. Cold Spring Harbor Laboratory Press
4. Jares P. 2006. Ultrastruct Pathol 30: 209-19
5. Tuschl T. 2001. Chembiochem 2: 239-45
6. Ed Harlow DL. 1988. Cold Spring Harbor Laboratory Press
7. Janin J, Wodak SJ. 2002. Adv Protein Chem 61: 1-8
8. Karin M, Hunter T. 1995. Curr Biol 5: 747-57
9. Stelzl U, Wanker EE. 2006. Curr Opin Chem Biol 10: 551-8
10. Calderwood DA. 2004. J Cell Sci 117: 657-66
11. Manso AM, Elsherif L, Kang SM, Ross RS. 2006. Cardiovasc Res 69: 574-84
12. Seiki M. 2003. Cancer Lett 194: 1-11
13. Springer TA, Wang JH. 2004. Adv Protein Chem 68: 29-63
14. Braga VM. 2002. Curr Opin Cell Biol 14: 546-56
15. Gumbiner BM. 2005. Nat Rev Mol Cell Biol 6: 622-34
16. Halbleib JM, Nelson WJ. 2006. Genes Dev 20: 3199-214
17. Sallee JL, Wittchen ES, Burridge K. 2006. J Biol Chem 281: 16189-92
18. Holmbeck K, Bianco P, Yamada S, Birkedal-Hansen H. 2004. J Cell Physiol 200: 11-9
19. Larsen M, Artym VV, Green JA, Yamada KM. 2006. Curr Opin Cell Biol 18: 463-71
20. Wang YL. 2007. Sci STKE 2007: pe10
21. Holmes TC. 2002. Trends Biotechnol 20: 16-21
22. Shin H. 2007. Biomaterials 28: 126-33
23. Kirkpatrick CJ, Krump-Konvalinkova V, Unger RE, Bittinger F, Otto M, Peters K. 2002.
Biomol Eng 19: 211-7
24. Minuth WW, Schumacher K, Strehl R, Kloth S. 2000. J Biomater Sci Polym Ed 11: 495-
522
25. Wilson CJ, Clegg RE, Leavesley DI, Pearcy MJ. 2005. Tissue Eng 11: 1-18
26. Even-Ram S, Yamada KM. 2005. Curr Opin Cell Biol 17: 524-32
27. Cukierman E, Pankov R, Stevens DR, Yamada KM. 2001. Science 294: 1708-12
28. Niwa H. 2001. Cell Struct Funct 26: 137-48
29. Noggle SA, James D, Brivanlou AH. 2005. Stem Cell Rev 1: 111-8
30. Feng GS. 2007. Cell Res 17: 37-41
31. Kleber M, Sommer L. 2004. Curr Opin Cell Biol 16: 681-7
32. Zhang J, Li L. 2005. Dev Biol 284: 1-11
33. Lukyanov KA, Chudakov DM, Lukyanov S, Verkhusha VV. 2005. Nat Rev Mol Cell Biol
6: 885-91
34. Shaner NC, Steinbach PA, Tsien RY. 2005. Nat Methods 2: 905-9
35. Tsien RY. 1998. Annu Rev Biochem 67: 509-44
36. Giepmans BN, Adams SR, Ellisman MH, Tsien RY. 2006. Science 312: 217-24
37. Lippincott-Schwartz J, Patterson GH. 2003. Science 300: 87-91
38. Zhang J, Campbell RE, Ting AY, Tsien RY. 2002. Nat Rev Mol Cell Biol 3: 906-18