Biomaterials for tissue engineering slideshareBukar Abdullahi
An overview of Tissue Engineering with some basics in Biomaterials and Synthetic Polymers. Further references should be considered as I presented this a specific target audience.
Biomaterials for tissue engineering slideshareBukar Abdullahi
An overview of Tissue Engineering with some basics in Biomaterials and Synthetic Polymers. Further references should be considered as I presented this a specific target audience.
SeedEZ 3D cell culture application notes - gel and drug embeddingLena Biosciences
SeedEZ 3D cell culture application notes - gel and drug embedding. Many inert polymers used as scaffolds for 3D cell cultures and colony formation are also used in drug delivery systems both in vitro and in vivo. Read this practical guide to learn how SeedEZ lets you merge these two worlds in order to integrate 3D cell cultures into standard drug delivery and testing applications.
By incorporating or adding drugs to SeedEZ, or in polymer matrices embedded in SeedEZ, dosage forms which release a drug over a period of time may be prepared in a desired shape and size. More importantly, all SeedEZ-based dosage forms may be tested in situ, with cells in a 3D cell culture. SeedEZ wicks most sol-state hydrogels, hydrogel precursors, semisolid media, excipient formulations, pharmaceuticals and test compounds. As a result, SeedEZ offers a novel 3D framework for (A) development of sustained release drug delivery systems that are simple to make and convenient to use in vitro; (B) localized or distributed drug delivery into 3D cell cultures using spot-a-culture and spot-a-drug approach, wick, dip or SeedEZ-stack method; (C) gradient formation and testing of drug combination strategies; (D) quality control testing and assurance; and (E) development of test platforms for quasi-steady drug release.
Notably, in most diffusion-driven drug delivery systems, a drug release rate declines in time. A degradable polymer matrix embedded in SeedEZ may enable quasi-steady drug release from a defined volume, defined by SeedEZ, when the matrix degradation rate is adjusted to compensate for this decline via increased drug permeability from the SeedEZ/polymer matrix system.
The application note covers use of common biomaterials, including extracellular matrix hydrogels (Collagen and Matrigel), gels from natural sources for spheroid cultures and controlled drug release (Agarose, Alginate, Methylcellulose, Gelatin), and synthetic materials such as Poloxamers (Pluronic - used for cell encapsulation, drug delivery and pharmaceutical formulations), and Carbomers used in ocular, transdermal, oral and nasal delivery systems.
Stem cells and nanotechnology in regenerative medicine and tissue engineeringDr. Sitansu Sekhar Nanda
Alexis Carrel, winner of the Nobel Prize in Physiology or Medicine in 1912 and the father of whole-organ transplant, was the first to develop a successful technique for end to end arteriovenous anastomosis in transplantation.
SeedEZ 3D cell culture application notes - gel and drug embeddingLena Biosciences
SeedEZ 3D cell culture application notes - gel and drug embedding. Many inert polymers used as scaffolds for 3D cell cultures and colony formation are also used in drug delivery systems both in vitro and in vivo. Read this practical guide to learn how SeedEZ lets you merge these two worlds in order to integrate 3D cell cultures into standard drug delivery and testing applications.
By incorporating or adding drugs to SeedEZ, or in polymer matrices embedded in SeedEZ, dosage forms which release a drug over a period of time may be prepared in a desired shape and size. More importantly, all SeedEZ-based dosage forms may be tested in situ, with cells in a 3D cell culture. SeedEZ wicks most sol-state hydrogels, hydrogel precursors, semisolid media, excipient formulations, pharmaceuticals and test compounds. As a result, SeedEZ offers a novel 3D framework for (A) development of sustained release drug delivery systems that are simple to make and convenient to use in vitro; (B) localized or distributed drug delivery into 3D cell cultures using spot-a-culture and spot-a-drug approach, wick, dip or SeedEZ-stack method; (C) gradient formation and testing of drug combination strategies; (D) quality control testing and assurance; and (E) development of test platforms for quasi-steady drug release.
Notably, in most diffusion-driven drug delivery systems, a drug release rate declines in time. A degradable polymer matrix embedded in SeedEZ may enable quasi-steady drug release from a defined volume, defined by SeedEZ, when the matrix degradation rate is adjusted to compensate for this decline via increased drug permeability from the SeedEZ/polymer matrix system.
The application note covers use of common biomaterials, including extracellular matrix hydrogels (Collagen and Matrigel), gels from natural sources for spheroid cultures and controlled drug release (Agarose, Alginate, Methylcellulose, Gelatin), and synthetic materials such as Poloxamers (Pluronic - used for cell encapsulation, drug delivery and pharmaceutical formulations), and Carbomers used in ocular, transdermal, oral and nasal delivery systems.
Stem cells and nanotechnology in regenerative medicine and tissue engineeringDr. Sitansu Sekhar Nanda
Alexis Carrel, winner of the Nobel Prize in Physiology or Medicine in 1912 and the father of whole-organ transplant, was the first to develop a successful technique for end to end arteriovenous anastomosis in transplantation.
this presentation depicts the usefulness of single cell profiling in crop plant for identifying novel gene sources which can be used for crop improvement
Effect of Surface Engineering on Stem Cells.pdfaman15nanavaty
A thorough review covering the nascent domain of Surface Engineering on Stem Cells. This short review will cover basic details of Stem Cell Engineering and Cell Culture Surface Engineering.
Our third webinar in the MDC Connects Series 2021 | A Guide to Complex Medicines.
This slide deck gives an overview of the early assessment of Prototype Nanomedicine Nano Bio Interactions.
Zahra Rattray, University of Strathclyde
This is my short presentation in one of my university classes. It's obvious that the future of the stem cell biology is tightly engaged with organoids and they will absolutely change the way science is going to.
Kind regards
Shahin Ahmadian
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.
An organ-on-a-chip (OOC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems, a type of artificial organ. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.
New Explore Careers and College Majors 2024.pdfDr. Mary Askew
Explore Careers and College Majors is a new online, interactive, self-guided career, major and college planning system.
The career system works on all devices!
For more Information, go to https://bit.ly/3SW5w8W
2. Objective
Results-oriented and self-motivated biomedical engineering professional
seeking R&D position in Biotech/Medical Device industry
Key Qualifications
•Over
10 years solid professional experience in cell culture, target drug
delivery and cancer diagnosis device development
• Indepth knowledge of using principle of cell biology, molecular biology,
materials science and engineering to solve biotechnology problems
• Excellent hands on skills of cell culture, cell based assay, polymer
synthesis, drug loaded nanoparticle preparation and medical device
fabrication
• Strong analytical, problem solving and multitasking skills coupled with
ability to work both independently and as part of a multidisciplinary team
• Eligible to working for any employers in USA without sponsorship
3. Education
2006~2011
Ph.D. in Chemical Engineering,
University of South Carolina,
Columbia, SC
2003~06
M.S. in Biochemical
Engineering, Dalian Institute of
Chemical Physics. P.R. China
1999~2003
B.S. in Life Sciences,
University of Science and
Technology of China
4. Technical Skills
Cell Biology Techniques:
mammalian and bacteria cell culture, cell-based
toxicity test, bacteria transformation, cell
transfection, flow cytometry, fluorescent
microscopy, confocal microscopy
Immunology Techniques:
immunostaining, ELISA development
Molecular Biology
Techniques:
RNA and DNA Extraction, PCR, DNA Gel Analysis,
Western Blotting
Animal Manipulation:
handling and breeding, primary cells
generation, injection, blood withdrawal,
survival surgery and post-operative care of
rodents
GPC, HPLC, LC-MS, NMR, SEM, spectrophotometry
Analytical Techniques:
5. Professional Experience
Postdoctoral Researcher, The Ohio State University,
Department of Chemical& Biomolecular Engineering
May 2011-present
•• Develop a non-invasive detection method, tethered
Develop a non-invasive detection method, tethered
cationic lipoplex nanoparticles biochip, to simultaneously captures
cationic lipoplex nanoparticles biochip, to simultaneously captures
and characterize tumor biomarkers such as circulating tumors cells
and characterize tumor biomarkers such as circulating tumors cells
and exosomes.
and exosomes.
•• Successfully generated induced neuron from embryonic and adult
Successfully generated induced neuron from embryonic and adult
fibroblast cells with nonviral method, achieving low tumorgenicity
fibroblast cells with nonviral method, achieving low tumorgenicity
and mutation compared with traditional viral method
and mutation compared with traditional viral method
•• Fabricated a multifunctional device by combining release chamber
Fabricated a multifunctional device by combining release chamber
and cell microencapsulation for therapeutic and pharmaceutics
and cell microencapsulation for therapeutic and pharmaceutics
applications, such as treating diabetics and monitoring inflammation
applications, such as treating diabetics and monitoring inflammation
6. Professional Experience
Research Assistant
August
2006-May 2011
University of South Carolina, Department of Chemical
Engineering
“Tissue engineered bone regeneration in a biodegradable osteoinductive scaffold”
“Tissue engineered bone regeneration in a biodegradable osteoinductive scaffold”
•• Studied methods for synthesis of complex organic molecules, such as macromers and peptides
Studied methods for synthesis of complex organic molecules, such as macromers and peptides
•• Implemented chemical variations to polymers to introduce functionality
Implemented chemical variations to polymers to introduce functionality
•• Studied techniques for culture, differentiation, and analysis of bone marrow stromal cells
Studied techniques for culture, differentiation, and analysis of bone marrow stromal cells
•• Characterized the biocompatibility and osteogenic potential of scaffolds in vivo (subcutaneous
Characterized the biocompatibility and osteogenic potential of scaffolds in vivo (subcutaneous
implantation,
implantation,
critical femoral defect of rats, and others ))
critical femoral defect of rats, and others
““
7. Professional Experience
Research Assistant
August
2006-May 2011
University of South Carolina, Department of Chemical
Engineering
“Multi-functional nanoparticles for drug delivery in vitro and in vivo study”
“Multi-functional nanoparticles for drug delivery in vitro and in vivo study”
•• Conjugated and encapsulated rhBMP-2 in NPs for differentiation of bone marrow stromal
Conjugated and encapsulated rhBMP-2 in NPs for differentiation of bone marrow stromal
cells
cells
•• Encapsulated Paclitaxel in biodegradable self-assembled core-shell poly(lactide-coEncapsulated Paclitaxel in biodegradable self-assembled core-shell poly(lactide-coglycolide ethylene oxide fumarate) nanoparticles for targeted anti-tumor drug delivery
glycolide ethylene oxide fumarate) nanoparticles for targeted anti-tumor drug delivery
““
8. Professional Experience
Research Assistant
August 2003May 2006
Dalian Institute of Chemical Physics
• Fabricated artificial pancreas based on cell
• Fabricated artificial pancreas based on cell
microencapsulation technology to treat diabetes
microencapsulation technology to treat diabetes
• Transplanted alginate-chitosan-alginate
• Transplanted alginate-chitosan-alginate
microencapsulated bovine chromaffin cells to mice
microencapsulated bovine chromaffin cells to mice
intraperitoneal cavity, achieving long term analgesic
intraperitoneal cavity, achieving long term analgesic
effect
effect
• Immobilized endostatin secreting CHO cells in
• Immobilized endostatin secreting CHO cells in
microcapsules and optimized production of endostatin
microcapsules and optimized production of endostatin
in bioreactors
in bioreactors
9. •
•
•
•
•
•
•
•
Publications
1. J. Ma, X. He, E. Jabbari. “Osteogenic Differentiation of Marrow Stromal Cells
on Random and Aligned Electrospun Poly(L-lactide) Nanofibers”. Annals of
Biomedical Engineering. 2011, 39(1): 14-25
2. X. He, J. Ma, E. Jabbari. “Migration of marrow stromal cells in response to
sustained release of stromal-derived factor-1α from poly(lactide ethylene oxide
fumarate) hydrogels”. International Journal of Pharmaceutics. 2010, 390(2):107116
3. W. Xu, J. Ma, X. He, E. Jabbari. “Material properties and osteogenic
differentiation of marrow stromal cells on fiber-reinforced laminated hydrogel
nanocomposites”. Acta Biomaterialia. 2010, 6(6):1992-2002
4. A.E. Mercado, J. Ma, X. He, E. Jabbari. “Release characteristics and osteogenic
activity of recombinant human bone morphogenetic protein-2 grafted to novel selfassembled poly(lactide-co-glycolide fumarate) nanoparticles”. J. Control. Release.
2009, 140: 148-156.
5. X. He, J. Ma, A. E. Mercado, W. Xu, E. Jabbari. “Cytotoxicity of Paclitaxel in
Biodegradable Self-Assembled Core-Shell Poly(Lactide-Co-Glycolide Ethylene Oxide
Fumarate) Nanoparticles”. Pharm. Res. 2008, 25(7):1552-1562.
6. X. He, J. Ma, E. Jabbari. “Effect of grafting RGD and BMP-2 protein-derived
peptides to a hydrogel substrate on osteogenic differentiation of marrow stromal
cells”. Langmuir. 2008, 24 (21): 12508–12516
7. S. Moeinzadeh, S. Khorasani, J. Ma, X. He, E. Jabbar. “Synthesis and gelation
characteristics of photo-crosslinkable star Poly(ethylene oxide-co-lactideglycolide acrylate) macromonomers”. Polymer. 2011,52(18): 3887–3896
8. J. Zhou, Y. Zhang, W. Wang, J. Ma, H. Zhang, X. Guo, X. Ma. “The Effect of
10. •
•
•
•
•
•
•
•
Publications
1. J. Ma, X. He, E. Jabbari. “Osteogenic Differentiation of Marrow Stromal Cells
on Random and Aligned Electrospun Poly(L-lactide) Nanofibers”. Annals of
Biomedical Engineering. 2011, 39(1): 14-25
2. X. He, J. Ma, E. Jabbari. “Migration of marrow stromal cells in response to
sustained release of stromal-derived factor-1α from poly(lactide ethylene oxide
fumarate) hydrogels”. International Journal of Pharmaceutics. 2010, 390(2):107116
3. W. Xu, J. Ma, X. He, E. Jabbari. “Material properties and osteogenic
differentiation of marrow stromal cells on fiber-reinforced laminated hydrogel
nanocomposites”. Acta Biomaterialia. 2010, 6(6):1992-2002
4. A.E. Mercado, J. Ma, X. He, E. Jabbari. “Release characteristics and osteogenic
activity of recombinant human bone morphogenetic protein-2 grafted to novel selfassembled poly(lactide-co-glycolide fumarate) nanoparticles”. J. Control. Release.
2009, 140: 148-156.
5. X. He, J. Ma, A. E. Mercado, W. Xu, E. Jabbari. “Cytotoxicity of Paclitaxel in
Biodegradable Self-Assembled Core-Shell Poly(Lactide-Co-Glycolide Ethylene Oxide
Fumarate) Nanoparticles”. Pharm. Res. 2008, 25(7):1552-1562.
6. X. He, J. Ma, E. Jabbari. “Effect of grafting RGD and BMP-2 protein-derived
peptides to a hydrogel substrate on osteogenic differentiation of marrow stromal
cells”. Langmuir. 2008, 24 (21): 12508–12516
7. S. Moeinzadeh, S. Khorasani, J. Ma, X. He, E. Jabbar. “Synthesis and gelation
characteristics of photo-crosslinkable star Poly(ethylene oxide-co-lactideglycolide acrylate) macromonomers”. Polymer. 2011,52(18): 3887–3896
8. J. Zhou, Y. Zhang, W. Wang, J. Ma, H. Zhang, X. Guo, X. Ma. “The Effect of