4. Chapter 1 Safety Measures in Lab
1.1 Self Protection Equipment
1.2 Disposable of Waste
5. You are able to:
Explain functions at least 9 self-protective equipment
Identify and justify substances that:
(a)Can be disposed in the sink
(b)Cannot be disposed into the sink
Describe at least 3 methods in managing biological
wastes correctly
Explain the steps to manage accidents in laboratory:
(a)4 steps – chemical spill
(b)4 steps – mercury hazard
7. Dressing for Lab
Below include an image in each box of appropriate lab dress.
Lab coat
Closed toe Shoe
Safety Glasses
Chemical Resistant
Gloves
Long Pants
Face mask
8.
9.
10.
11.
12.
13. 1
Wash off any spilled chemicals or toxic
substances
2 To handle biological samples
3
To avoid accidents due to glass breakage
or chemical spill
4 Provide filtered air ventilation
5
Irrigation for eye upon in contact with
chemical
6
Avoid the smell of harmful gases such as
chlorine
7
To protect eyes from direct chemical
contact
8
To protect the body and clothes from
chemical
9 Protect hands from chemicals
A Closed toe shoes
B Laminar flow cabinet
C Lab coat
D
Chemical Resistant
Gloves
E Safety shower
F Fume chamber
G Safety glasses/goggles
H Face mask
I Eyewash station
15. Waste Disposal
Waste can be divided into to groups:
1st Can be disposed into
the sink
2nd Cannot be disposed
into the sink
16. Chemical with pH 5 to 9 Toxic substances
Solid wastes (glass) Radioactive wastes
Volatile substances
Chemical with pH less than 5 or
more than 9
Chemical solution (acids) Heavy metals e.g. mercury
Organic wastes e.g. carcasses
Solution with low concentration &
harmless
Radioactive wastes
18. Chemical with pH 5 to 9 Toxic substances
Solid wastes (glass) Radioactive wastes
Volatile substances
Chemical with pH less than 5 or
more than 9
Chemical solution (acids) Heavy metals e.g. mercury
Organic wastes e.g. carcasses
Solution with low concentration &
harmless
Radioactive wastes
19. Handling Biological Waste
There are three category for Biological Waste
• Kept temporarily in a safe place
Category A
• Put into leak proof autoclave bags and
autoclave it
Category B
• Carefully wrapped and stored temporarily in
freezer
Category C
20. A: Disposed into ‘sharp’ bin. No
sterilization needed.
B: Packed into ‘biohazard’ bag,
autoclaved and then disposed into
biohazard bin
C: Wrapped in tissue, packed in
‘biohazard’ bag and frozen
D: Autoclaved and disposed
21. In the Event of a Lab
Accident…
Chemical spill
Inform teacher/lab
assistant
Probihated area
Sand on the spill
Gather the spillage and
disposed safely
Mercury spill
Inform teacher/lab
assistant
Probihited area
Sulphur on mercury spill
Call fire brigade
23. You are able to:
Describe the types of fire extinguisher and its
corresponding use on class of fire
Explain the method of using fire extinguisher - PASS
Carry out an audit on the fire extinguishers at school
Create a simple fire extinguisher that works by using
materials available at home
24.
25.
26.
27. A B C D E F
Water
Foam
Dry
powder
CO2
Fire
Extinguisher
Type
28. A B C D E F
Water
Foam
Dry
powder
CO2
Fire
Extinguisher
Type
