Hexokinase

1. Hexokinase
A project submitted in partial fulfillment of the requirements for the degree of
MSc. Biotechnology
by
Sura altaie
Yasameen hameed
Zainab almaryani
Supervised by
Dr. Adib charafeddine
American University of Science and Technology
2022

2. Hexokinase (HK) is a regulated enzyme that
catalyzes the first step in the use of glucose
according to the following reaction
Hexokinase
GLUCOSE + ATP → G6P + ADP

3. HEXOKINASE ENZYME CLASS
Hexokinase is a protein which is
classified under the main grouping of
a transferase enzyme. Its structure was
first determined from yeast by tom steitz
at yale university. Hexokinase is the first
enzyme in the glycolytic pathway, and it
converts glucose into glucose-6-
phosphate.

4. TYPES OF MAMMALIAN HEXOKINASE
There are four important mammalian
hexokinase isozymes that vary in subcellular
locations and kinetics with respect to different
substrates and conditions, and physiological
function. They are designated hexokinases I,
II, III, and IV or hexokinases A, B, C, and D

5. HOW IS HEXOKINASE ACTIVATED?
Hexokinase activates glycolysis by
phosphorylating glucose. Since the
phosphorylation of glucose to glucose-6
phosphate is the rate limiting step of glucose
metabolism, hexokinase has a very important
role in regulating healthy glucose levels in the
human body

6. The cause of hexokinase deficiency
is linked to mutations of the HK gene
and the encoding of the HK enzyme. The
result of the mutations lead to reduction
in HK activity.

7. Thank you