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‫بسم ا الرحمن الرحيم‬
GENUS:
CAMPYLOBACTER
Prof. Khalifa Sifaw Ghenghesh
• Slender, Helically Curved
Rods.
• Corkscrew-Darting Motility.
• Oxidase: +ve
• Microaerophilic >
–Campy.
–Campy.
–Campy.
–Campy.

jejuni
coli
lari (Campy. laridis)
Fetus

• Arcobacter >> A. butzleri
Campylobacter fetus
Leifson flagella stain
Campylobacter jejuni
Campylobacter jejuni
Campylobacter jejuni

thin, comma-, S-, or gull-winged shaped forms
(48 h culture)
VIRULENCE FACTORS
• Motility
• Adherence
• Invasion
• Toxin Production
–Cytotoxins:
–Enterotoxins
DISEASE
• in Animal:
• in Man:
– Diarroeal Illness:
– Guillain-Barre Syndrome (GBS):
LABORATORY DIAGNOSIS
• Specimen:
• Culture:
– Selective media >> Skirrow's >

• Incubation:
– Temp.
– microaerobic gas generation packs

• Identification:
– Oxidase, Gram stain, Hippurate hydrolysis
Blood agar plate culture of
Campylobacter fetus s. intestinalis
TREATMENT
• Campylobacter enteritis
– Self-limiting
– Fluid and electrolyte replacement

• Erythromycin
• Fluoroquinolones
– Ciprofloxacin
EPIDEMIOLOGY
• Incidence
• Source of Transmission
– Poultry and Raw Meats
– Other routes of Infection
CONTROL
• Education:
– Keep food that will be eaten raw, such as vegetables, from
becoming contaminated by raw animal-derived food products.
– Thoroughly cook all food products from animals, especially
poultry, and avoid consuming unpasteurized milk, or other
unpasteurized products.
– Cases should not prepare food for other individuals or attend
child care until diarrhea has resolved.
– Educate case and household contacts on proper hand washing
techniques.
– Always wash hands thoroughly with soap and water before
eating or preparing food, after using the toilet, after changing
diapers, and after touching pets or other animals (especially
puppies and kittens with diarrhea).
– After changing diapers, wash your hands AND the child’s
hands.
– In a childcare setting, dispose of stool and soiled diapers in a
sanitary manner.
CONTROL
•

Food Handlers:
– Food handlers should be excluded from work until diarrhea has
resolved.
– While individual circumstances may vary, cases are generally not
required to provide two negative stools to return to work.
– If a case has questionable hygienic practices or there are other
concerns, a food handler should be excluded from work until two
negative stool cultures have been obtained at least 24 hours apart.
– In an outbreak situation, negative cultures may be required to
return to food handling.
Information on children with Campylobacterassociated diarrhoea in Tripoli.
________________________________________________
Pt.
Age/
Stool with
Vomiting Fever
dehydSex mucus blood
ration
--------------------------------------------------------------------------------1.
3/F
-+
--+
2.
7/M
--+
+
-3.
8/F
+
----4.
9/F
----+
5.
4/M
+
-+
--6.
14/M
+
--+
----------------------------------------------------------------------------------
‫بسم ا الرحمن الرحيم‬

GENUS:
HELICOBACTER
Prof. Khalifa Sifaw Ghenghesh
• Spiral, curved/straight G-ve
rods.
• Motile.
• Microaerophilic / 37oC.
• Oxidase +ve
• > 15 species of Helicobater
–H. pylori
Helicobacter pylori
3D morphology
Helicobacter pylori
showing typical thin, comma- or S-shaped
forms (72 h culture).
1. H. pylori
• Natural Habitat:
- Human gastric mucosa.
- Dental plaque.
- Human faeces.

• Transmission:
- Faecal-oral or Oral-oral route.
- Flies ???
• Virulence Factors:
–Urease, Phospholipase A, etc.

• Clinical Significance:
–Duodenal Ulcer & Gastritis.
–Gastric adenocarcinoma.
LABORATORY DIAGNOSIS
1. Invasive Tests:
–
–
–
–

Specimen: Gastric mucosa.
Microscopy:
Culture:
Selective media
Biopsy urease test:

2. Non-Invasive Tests:
– Serology: Abs to HP
– Urease breath test:
TREATMENT
Twice-Daily PPI-Based Triple Therapies
• Cure rate 90%: 2 weeks (? 10 days) in U.S., 1 week
outside U.S.
• Omeprazole 20 mg bid or Lansoprazole 30 mg bid
• Clarithromycin 500 mg bid
• Amoxicillin 1 g bid or Metronidazole 500 mg bid

Twice-Daily RBC-Based Triple Therapy
• Cure rate 90% in 2 weeks
• Ranitidine bismuth citrate 400 mg bid
• Clarithromycin 500 mg bid
• Amoxicillin 1 g bid (?metronidazole 500 mg bid)
FDA-Approved (USA) Dual Therapies
• Cure rate 70% at 2 weeks
• Omeprazole 40 mg qd
• Clarithromycin 500 mg tid
• Cure rate 80% at 2 weeks
• Ranitidine bismuth citrate 400 mg bid
• Clarithromycin 500 mg tid
H. cinaedi
H. fennelliae
• Natural Habitat:
– Intestinal tract in rodents.
– Human faeces & rectal cultures.

• Clinical significance:
– Proctocolitis, Proctitis, Enteritis,
Bacteraemia.

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Lecture 20 Campylocbacter and Helicobacter

  • 1. ‫بسم ا الرحمن الرحيم‬ GENUS: CAMPYLOBACTER Prof. Khalifa Sifaw Ghenghesh
  • 2. • Slender, Helically Curved Rods. • Corkscrew-Darting Motility. • Oxidase: +ve • Microaerophilic > –Campy. –Campy. –Campy. –Campy. jejuni coli lari (Campy. laridis) Fetus • Arcobacter >> A. butzleri
  • 6. Campylobacter jejuni thin, comma-, S-, or gull-winged shaped forms (48 h culture)
  • 7. VIRULENCE FACTORS • Motility • Adherence • Invasion • Toxin Production –Cytotoxins: –Enterotoxins
  • 8. DISEASE • in Animal: • in Man: – Diarroeal Illness: – Guillain-Barre Syndrome (GBS):
  • 9. LABORATORY DIAGNOSIS • Specimen: • Culture: – Selective media >> Skirrow's > • Incubation: – Temp. – microaerobic gas generation packs • Identification: – Oxidase, Gram stain, Hippurate hydrolysis
  • 10. Blood agar plate culture of Campylobacter fetus s. intestinalis
  • 11. TREATMENT • Campylobacter enteritis – Self-limiting – Fluid and electrolyte replacement • Erythromycin • Fluoroquinolones – Ciprofloxacin
  • 12. EPIDEMIOLOGY • Incidence • Source of Transmission – Poultry and Raw Meats – Other routes of Infection
  • 13. CONTROL • Education: – Keep food that will be eaten raw, such as vegetables, from becoming contaminated by raw animal-derived food products. – Thoroughly cook all food products from animals, especially poultry, and avoid consuming unpasteurized milk, or other unpasteurized products. – Cases should not prepare food for other individuals or attend child care until diarrhea has resolved. – Educate case and household contacts on proper hand washing techniques. – Always wash hands thoroughly with soap and water before eating or preparing food, after using the toilet, after changing diapers, and after touching pets or other animals (especially puppies and kittens with diarrhea). – After changing diapers, wash your hands AND the child’s hands. – In a childcare setting, dispose of stool and soiled diapers in a sanitary manner.
  • 14. CONTROL • Food Handlers: – Food handlers should be excluded from work until diarrhea has resolved. – While individual circumstances may vary, cases are generally not required to provide two negative stools to return to work. – If a case has questionable hygienic practices or there are other concerns, a food handler should be excluded from work until two negative stool cultures have been obtained at least 24 hours apart. – In an outbreak situation, negative cultures may be required to return to food handling.
  • 15. Information on children with Campylobacterassociated diarrhoea in Tripoli. ________________________________________________ Pt. Age/ Stool with Vomiting Fever dehydSex mucus blood ration --------------------------------------------------------------------------------1. 3/F -+ --+ 2. 7/M --+ + -3. 8/F + ----4. 9/F ----+ 5. 4/M + -+ --6. 14/M + --+ ----------------------------------------------------------------------------------
  • 16. ‫بسم ا الرحمن الرحيم‬ GENUS: HELICOBACTER Prof. Khalifa Sifaw Ghenghesh
  • 17. • Spiral, curved/straight G-ve rods. • Motile. • Microaerophilic / 37oC. • Oxidase +ve • > 15 species of Helicobater –H. pylori
  • 19. Helicobacter pylori showing typical thin, comma- or S-shaped forms (72 h culture).
  • 20. 1. H. pylori • Natural Habitat: - Human gastric mucosa. - Dental plaque. - Human faeces. • Transmission: - Faecal-oral or Oral-oral route. - Flies ???
  • 21. • Virulence Factors: –Urease, Phospholipase A, etc. • Clinical Significance: –Duodenal Ulcer & Gastritis. –Gastric adenocarcinoma.
  • 22.
  • 23. LABORATORY DIAGNOSIS 1. Invasive Tests: – – – – Specimen: Gastric mucosa. Microscopy: Culture: Selective media Biopsy urease test: 2. Non-Invasive Tests: – Serology: Abs to HP – Urease breath test:
  • 25. Twice-Daily PPI-Based Triple Therapies • Cure rate 90%: 2 weeks (? 10 days) in U.S., 1 week outside U.S. • Omeprazole 20 mg bid or Lansoprazole 30 mg bid • Clarithromycin 500 mg bid • Amoxicillin 1 g bid or Metronidazole 500 mg bid Twice-Daily RBC-Based Triple Therapy • Cure rate 90% in 2 weeks • Ranitidine bismuth citrate 400 mg bid • Clarithromycin 500 mg bid • Amoxicillin 1 g bid (?metronidazole 500 mg bid)
  • 26. FDA-Approved (USA) Dual Therapies • Cure rate 70% at 2 weeks • Omeprazole 40 mg qd • Clarithromycin 500 mg tid • Cure rate 80% at 2 weeks • Ranitidine bismuth citrate 400 mg bid • Clarithromycin 500 mg tid
  • 27. H. cinaedi H. fennelliae • Natural Habitat: – Intestinal tract in rodents. – Human faeces & rectal cultures. • Clinical significance: – Proctocolitis, Proctitis, Enteritis, Bacteraemia.