Molecular analysis of plants transformed biolistically in general reveals a complex pattern of trans-gene, indicating the integration of multiple copies of the bombarded DNA.

2. The Biolistic Method
Micro projectiles
tungsten Gold
DNA
Target Cell
Acceleration
Gunpowder
High-pressure gases
He CO2
Molecular analysis of plants
transformed biolistically in
general reveals a complex
pattern of trans-gene,
indicating the integration of
multiple copies of the
bombarded DNA.

3. (a)A wide variety of types of explants can be used
to undergo bombardment and obtain fertile
plants.
(b) There is no need for specialized
transformation vectors.
(c) This is the only reliable method for
chloroplast transformation.
The Biolistic Method

4. The Biolistic Method
This isn't 100% effective all of the time, some cells take up
the desired genes and begin to express them

5. The Biolistic Method
The cells that take up the genes can be identified with the
special "marker" gene.
This biolistic method has been used in rice to gain
resistance to the red stripe virus.

6. Other Transformation Methods
Polyethylene glycol (PEG).
Calcium phosphate.
Electroporation.
Maize

7. Low reproducibility.
Regeneration of plants.
The microinjection technique employs immobilized cells
into which the DNA is internalized individually
Other Transformation Methods