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Leocadio Sebastian
Regional Program Leader, CCAFS-SEA
Projected Temperature
Temperature(oC)
Ho Chi
Minh
City
Manila
Precipitation(mm/day)
Ho Chi
Minh
City
Manila
Temperature(oC) Projected Precipitation
Source: Work done by Tropical Marine Science Institute, NUS
AnnualMaximumDaily
Precip(mm/day)
Uncertainties in Rainfall
Source: Work done by Tropical Marine Science Institute, NUS, 2016
Climate
Vulnerability
over
Southeast
Asia
Source: Yusuf and Francisco. 2009.
Economy and Environment Program for
Southeast Asia
• Floods
• Droughts
• Sea level rise
• Landslides
• Cyclones
Climate Hazard Hotspots and Dominant Hazards
Source: Yusuf and Francisco. 2009. Economy and Environment Program for Southeast Asia
Climate hazard hotspots Dominant hazards
Northwestern Vietnam Droughts
Eastern coastal areas of Vietnam Cyclones, droughts
Mekong region of Vietnam Sea level rise
Bangkok and its surrounding area
in Thailand
Sea level rise, floods
Southern regions of Thailand Droughts, floods
The Philippines Cyclones, landslides, floods, droughts
Sabah state in Malaysia Droughts
Western and eastern of Java
Island, Indonesia
Droughts, floods, landslides, sea level
rise
Agriculture
contributes
to GHG
emission
(Magnitude and sources)
Source: Vermeulen et al (2012)
Food Security and
Environmental
Sustainability
Source: Foley et al. 2011. Solutions for a cultivated planet. Nature 478, pp. 337–342.
• Takes into account: food security,
adaptation and ecological footprint
• Development and ecological
footprint → green economy
• Foremost about development itself
and address smallholder concerns
• About outcomes, not just
technology or practices
• Context matters: CSA differs widely
• Crucial to deal with trade-offs
Climate Smart Agriculture (CSA)
Climate Impact to Climate-Smart Agriculture
Climate change ready crops
Tolerance of
complete
submergence
• Submergence affects over
20 million hectares in Asia
• Affected areas are highly
populated with poor farming
communities
• FR13A identified in 1970s
after screening 18,000
accessions
• SUB1 identified in mid-
1990s as the major gene
• Marker-aided backcrossing
(MABC) being used to
transfer Sub1 gene
(Source:A. M. Ismail, IRRI)
Salt-tolerant rice
• Major genes being
used for marker-
aided breeding
• Salt stress affecting
more than 16 million
hectares in Asia.
• Effect worsening with
climate change in
coastal deltas.
• Tolerant landraces
being used to
develop tolerant
varieties.
(Source:A. M. Ismail, IRRI)
Multiple trait varieties
• Submergence + stagnant flood
• Submergence + bacterial leaf blight
• Anaerobic germination + submergence
• Seedling stage salinity + reproductive stage salinity
• Submergence + seedling stage salinity
• Submergence + stagnant flooding
• Drought + phosphorus uptake
• Drought + high temperature + cold
+++ Good grain quality and pest and disease resistance Improved PSB Rc82 + GY traits
Marker-aided selection
High-nutrient rice
with zinc, iron, and beta-
carotene in the grain!
Genetic
Engineering
Genome editing for
iron biofortification
Targeting iron and bean genes to the
pre-determined locus in the BRRI
Dhan 28 genome using TALEN
(Transcription Activator-Like Effector
Nucleases).
The first generation genome-edited
Nsic Rc 238 lines with promoter
replacement obtained.
15-20 years of research needed
Engineering C4 Rice
C4-photosynthesis rice could increase yield,
water and nitrogen use efficiency by 30-50%.
Other Biotechnologies
• Genome association mapping –
explore the links between
genotype and phenotype
• RNA interference (RNAi)
technology -operates by
suppression of gene expression by
double-stranded RNA molecules
• Site-directed nuclease
mutagenetic techniques like
meganucleases, zinc-finger
nucleases
Integrate into the agricultural program
Challenges
1. Delays due to regulatory approvals
of GE crops
2. Bringing in PPP
• Combining the desired CC related traits in
commercially viable varieties requires
investment
3. Moving to action
• Developing a deployment strategy that will allow
availability of seeds where and when it is
needed
• Bringing to scale
• Contributing to GHG mitigation targets
• Contributing to sustainability goals
• Contributing to food security and nutrition
Ag Biotech
an important tool that is now being applied to
make CSA
• New sources of genes
• Transfer of genes – intra and
inter species
• Combining multiple genes
• Creating/activating novel traits
• CC adaptation/Resilience
• Productivity
• Mitigation of GHG emission
• Sustainability
• Food security and nutrition
Thank you!

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The role of biotechnologies in climate smart agriculture. l.sebastian

  • 2. Projected Temperature Temperature(oC) Ho Chi Minh City Manila Precipitation(mm/day) Ho Chi Minh City Manila Temperature(oC) Projected Precipitation Source: Work done by Tropical Marine Science Institute, NUS AnnualMaximumDaily Precip(mm/day)
  • 3. Uncertainties in Rainfall Source: Work done by Tropical Marine Science Institute, NUS, 2016
  • 4.
  • 5. Climate Vulnerability over Southeast Asia Source: Yusuf and Francisco. 2009. Economy and Environment Program for Southeast Asia • Floods • Droughts • Sea level rise • Landslides • Cyclones
  • 6. Climate Hazard Hotspots and Dominant Hazards Source: Yusuf and Francisco. 2009. Economy and Environment Program for Southeast Asia Climate hazard hotspots Dominant hazards Northwestern Vietnam Droughts Eastern coastal areas of Vietnam Cyclones, droughts Mekong region of Vietnam Sea level rise Bangkok and its surrounding area in Thailand Sea level rise, floods Southern regions of Thailand Droughts, floods The Philippines Cyclones, landslides, floods, droughts Sabah state in Malaysia Droughts Western and eastern of Java Island, Indonesia Droughts, floods, landslides, sea level rise
  • 7. Agriculture contributes to GHG emission (Magnitude and sources) Source: Vermeulen et al (2012)
  • 8. Food Security and Environmental Sustainability Source: Foley et al. 2011. Solutions for a cultivated planet. Nature 478, pp. 337–342.
  • 9. • Takes into account: food security, adaptation and ecological footprint • Development and ecological footprint → green economy • Foremost about development itself and address smallholder concerns • About outcomes, not just technology or practices • Context matters: CSA differs widely • Crucial to deal with trade-offs Climate Smart Agriculture (CSA)
  • 10. Climate Impact to Climate-Smart Agriculture
  • 12. Tolerance of complete submergence • Submergence affects over 20 million hectares in Asia • Affected areas are highly populated with poor farming communities • FR13A identified in 1970s after screening 18,000 accessions • SUB1 identified in mid- 1990s as the major gene • Marker-aided backcrossing (MABC) being used to transfer Sub1 gene (Source:A. M. Ismail, IRRI)
  • 13. Salt-tolerant rice • Major genes being used for marker- aided breeding • Salt stress affecting more than 16 million hectares in Asia. • Effect worsening with climate change in coastal deltas. • Tolerant landraces being used to develop tolerant varieties. (Source:A. M. Ismail, IRRI)
  • 14. Multiple trait varieties • Submergence + stagnant flood • Submergence + bacterial leaf blight • Anaerobic germination + submergence • Seedling stage salinity + reproductive stage salinity • Submergence + seedling stage salinity • Submergence + stagnant flooding • Drought + phosphorus uptake • Drought + high temperature + cold +++ Good grain quality and pest and disease resistance Improved PSB Rc82 + GY traits Marker-aided selection
  • 15. High-nutrient rice with zinc, iron, and beta- carotene in the grain! Genetic Engineering
  • 16. Genome editing for iron biofortification Targeting iron and bean genes to the pre-determined locus in the BRRI Dhan 28 genome using TALEN (Transcription Activator-Like Effector Nucleases). The first generation genome-edited Nsic Rc 238 lines with promoter replacement obtained.
  • 17. 15-20 years of research needed Engineering C4 Rice C4-photosynthesis rice could increase yield, water and nitrogen use efficiency by 30-50%.
  • 18. Other Biotechnologies • Genome association mapping – explore the links between genotype and phenotype • RNA interference (RNAi) technology -operates by suppression of gene expression by double-stranded RNA molecules • Site-directed nuclease mutagenetic techniques like meganucleases, zinc-finger nucleases
  • 19. Integrate into the agricultural program
  • 20. Challenges 1. Delays due to regulatory approvals of GE crops 2. Bringing in PPP • Combining the desired CC related traits in commercially viable varieties requires investment 3. Moving to action • Developing a deployment strategy that will allow availability of seeds where and when it is needed • Bringing to scale • Contributing to GHG mitigation targets • Contributing to sustainability goals • Contributing to food security and nutrition
  • 21. Ag Biotech an important tool that is now being applied to make CSA • New sources of genes • Transfer of genes – intra and inter species • Combining multiple genes • Creating/activating novel traits • CC adaptation/Resilience • Productivity • Mitigation of GHG emission • Sustainability • Food security and nutrition