Conventional extraction technique such as maceration and novel extraction techniques like Microwave Assisted Extraction method (MAE) were used for the extraction of mucilage from the stem of Abroma augusta. The mucilage content in the extracts obtained using both extraction methods. The conventional and novel extraction techniques were compared on the basis of various parameters like extraction time and extraction yield.

1. Presented by: CHANDRAKANTA MISRA
Roll No : 24201914021
Reg. No : 142420210021 of 2014-2015
Bengal College of Pharmaceutical Sciences & Research
2017
A Comparative Study of Conventional and
Microwave Assisted Extraction Method for the
Extraction of Mucilage from the Stem of
Abroma augusta.

2. Objective of work
 This project aims at comparison of Conventional
Extraction Method and Microwave Assisted
Extraction Method based on their principle,
strength and limitation to know the suitability and
economic feasibility of the methods.

3. Introduction
Conventional extraction technique such as maceration and
novel extraction techniques like Microwave Assisted
Extraction method (MAE) were used for the extraction of
mucilage from the stem of Abroma augusta. The mucilage
content in the extracts obtained using both extraction
methods. The conventional and novel extraction
techniques were compared on the basis of various
parameters like extraction time and extraction yield.

4. Plant Authentication
A herbarium sheet was prepared and was sent to
Botanical Survey of India, Central National
Herbarium, Shibpur, Howrah, West Bengal, India
for authentication, and the number is
“CNH/53a/2013/Tech.II/123”.

5. Plant Profile
 Abroma augusta – Pishacha Karpasa in Sanskrit and Ulat Kambal in
Hindi is used in Ayurveda for gynecological disorders, infertility
treatment, amenorrhoea,[1] and anti diabetic, anti hypertensive, anti
microbial. It is also called as Devil's cotton [2,3]. More information
about the plant are shown in Table.1
Scientific Classification
Kingdom Plantae
Order Malvales
Family Malvaceae
Genus Abroma
Species A. augusta
Table.1. Scientific classification

6. Experimental Section
Extraction of Mucilage by Conventional Method:
 The fresh stem was collected, washed with water, and
allowed to dry.
 The dried stems were then crushed, powdered for 5
minutes (5gm) , then 1gm powder was soaked in 40ml
distilled water in a beaker for 12 hours.
 Then filtered to remove the debris. Next centrifuged at
3000 rpm for 10 minutes, the recovered supernatant
was weighed and concentrated to volume of 10 ml and
weighed again.
 Later 40ml of ethanol was added and kept at 4°C for
12 hours at last centrifuged at 4500-8500 rpm for 10
minutes and precipitate was collected and weighed to
get the yield. [4,5].
Figure.1. Centrifuge

7. Experimental Section
Extraction by Microwave Assisted Extraction
Method:
The fresh stem was collected from a full grown plant,
washed with water, and allowed to dry.
The dried stems were then crushed, powdered for 5
minutes (5gm) and sieved, then 1gm powder was
soaked in 40ml distilled water in a beaker for 12 hours.
 Then the solution was kept in microwave oven along
with glass beads inside to prevent bumping ( microwave
irradiation at 160 watt intensity for 3 minutes).
Then the beaker was kept aside for 2 hrs to release the
mucilage, finally the material was filtered using muslin
bag. The precipitate was collected and yield was
measured.
Figure.2. Microwave

8. Calculation
 Calculation For Conventional Method [6]:
Initial Weight= 1gm, Final Weight=0.36gm
% yield= (initial weight/final weight)× 100
% yield=(1 / 0.36)×100
=0.36%
 Calculation For Microwave Assisted Extraction Method [6]:
Initial Weight= 1gm, Final Weight=0.46gm
% yield= (initial weight/final weight)× 100
% yield=(1 / 0.46)×100
=0.46%

9. Result and Discussion
Conventional Method
Sl.
No.
Initial
Weight(g)
Final
Weight(g)
Yeild %
1 1 gm 0.36gm 0.36
Microwave Assisted Extraction Method
Sl.
No.
Initial
weight(g)
Final
Weight(g)
Yeild %
1 1 gm 0.46gm 0.46
Table.2. Observation
Table.3. Observation
The observation (table.2,3) has proved that selected plant source (stem
of A. augusta) produced high yield of mucilage in shorter duration by
the developed microwave assisted extraction method when compared to
conventional method.

10. Comparison
Sl. No. Parameters Conventional
Method
Microwave Assisted
Extraction Method
1 Time Time
consuming
Takes less time
2 Solvent Solvent usage
is higher
Solvent usage is less
3 Procedure Complex Easier than
Conventional Method
4 Yield Less Higher
There are certain advantages and disadvantages in both methods
which I observed during the experiment are shown below .

11. Conclusion
Based on this study, it can be concluded that Microwave
Assisted Extraction method give better yield as compared
to conventional extraction method in shorter period of
time with less solvent consumption. Thus Microwave
Assisted Extraction method is superior to conventional
extraction method with respect to extraction time, solvent
consumption and extraction efficiency.

12. Reference
 [1]. Anonymus “The wealth of India” dictionary of Indian raw materials and
industrial products. The council of scientific and industrial research. (NISCAIR
press publisher; New Delhi 2006) 222.
 [2]. Gupta B, Nayak S, Solanki S (2011) Abroma augusta Linn: A review. Der
Pharmacia Sinica 2: 253-261.
 [3]. Rahmatullah M, Sadeak SMI, Bachar SC, Hossain MT, Montaha M, et al.
(2010) Brine Shrimp Toxicity Study of Different Bangladeshi Medicinal Plants.
Advances in Natural Applied Science 4: 163-173.
 [4]. Kokate CK. Practical Pharmacognosy. New Delhi: Vallabh Prakashan; 1999.
 [5]. Kulkarni GT, Gowthamarajan K, Brahmajirao G, Suresh B. Evaluation of
binding properties of selected natural mucilages. J Sci Ind Res., 61: 529-532
(2002).
 [6]Malviya R, Srivastava P, Kulkarni GT. (2011) Applications of Mucilages in
Drug Delivery - A Review. Advances in Biological Research. (5) 1-7.