This study examined using madre de cacao and guava leaf extracts as natural mosquito larvicides. The researchers extracted and fermented the leaves to create four treatments at different ratios. They conducted experiments applying the treatments to mosquito larvae and measured the average time to kill the larvae. The ANOVA results found no significant difference in killing time between the treatments. The study concluded that madre de cacao and guava leaf extracts have potential as effective natural larvicides.

1. Madre de cacao and guava
leaves extracts as mosquito
larvaecide
Members:
Israfil Bon B. Orbigoso
Heasha Mae E. Favor
Erick Jhan A. Sevillo

2. Rationale
- Philippines health has been alarmed as there has been an
increase in the mosquitoborne viruses, dengue and
chikungunya, early in 2023.

3. - Pesticides and Insecticides have been
associated with short and long-term effects on
human health.
Rationale

4. - Madre de cacao leaves are good source of coumarins, a
toxic substance that can kill almost all types of pest and
insect.
-Some of the phytochemicals identified in the leaf
extracts of guava like saponins, tannins, etc., have been
reported to posses potent mosquito larvicidal activity.
Rationale

5. This study aims to help:
-Public Health
Rationale

6. Limitations:
-No Controlled Treatment
Rationale

7. - The average time it will take to kill all mosquito larvae
when applied with extracts at different concentrations.
- If there is a significant difference between the time taken
to kill the mosquito larvae, when applied with extract at
different concentrations.
Research Questions

8. - Gathering of Materials
a) Larvaecide Production
Methodology

9. Methodology
a) Larvaecide Production
- Extracting and Fermenting
Leaves

10. Treatment
A ( 20:50)
Treatment
B (30:50)
Treatment
C (40:50)
Treatment
D (50:50)
Methodology
-Experimental Methods
-Treatment Application
b) Research Design

11. Methodology
c) Experimentation
- Conducting the Experiment

12. Methodology
c) Experimentation
- Gathering Data and
Analysis

13. Factor/Treatment Mean (seconds) Mean (min: sec: ms)
A (20:50 ratio) 1,751.87 29:11:87
B (30:50 ratio) 1,741.61 29:01:61
C (40:50 ratio) 1,743.78 29:03:78
D (50:50 ratio) 1,734.69 28:54:69
Average Time taken to kill mosquito
Findings of the Study

14. Sources of
Variation
Degree of
Freedom
Sum of
Square
Mean
Square
Computed
F
Tabulated
F
Treatment 3 733.856 244.6187 2.28ns 5% 1%
Error 12 1285.7998 107.14998 3.49 5.95
Total 15 2019.6558
ANOVA
Findings of the Study

15. - Madre De Cacao and Guava Leaves
Extract can be an effective Larvaecide.
Conclusion

16. “DENGUE IS NOW ONE OF THE MOST IMPORTANT TROPICAL DISEASES. WHILE IT
DOESN’T KILL THAT MANY PEOPLE, IT HAS TREMENDOUS ECONOMIC AND SOCIAL
IMPACT”
- DUANE GUBLER
THANK YOU