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Presented by –
Dr Anuradha M Sawant
AZOOSPERMIA – A CHALLENGE
FOR THE INFERTILITY
SPECIALIST
1
Married since 7
years
Cohabitating
since 3 years
No history of
use of any
contraception
Couple presented with inability to conceive
2
CASE HISTORY
26 years old Nulligravida
Wife of Serving Soldier
Hindu
Graduate
Homemaker
3
MENSTRUAL HISTORY
 Menarche- 14 years of age
 Previous Menstrual Cycles-
Irregular
3-5 days bleeding/ 60-90 days cycles
No dysmenorrhea
No passage of clots PV
4
OBSTETRIC HISTORY - Nulligravida
FAMILY HISTORY – Not significant
PAST HISTORY- Not significant
5
PERSONAL HISTORY
No Alcohol
No
Smoking
No Tobacco
use
No Drug
abuse
6
GENERAL PHYSICAL EXAMINATION
Overweight
Height- 160 cm / Weight- 70 kg /
BMI-27.3 kg/m2
No Pallor/ Icterus/ Edema/
Cyanosis/ Clubbing/
Lymphadenopathy/Galactorrhea/
Thyromegaly
Pulse:88 bpm
Blood Pressure:126/88mm Hg
Respiratory Rate- 18 bpm
SPO2- 99% RA
7
 HIRSUTISM – Present
Sum total score
of 10
(Ferriman -
Gallwey Score)
8
ACANTHOSIS NIGRICANS - seen
9
BREAST EXAMINATION
 TI
TANNER
Stage IV
10
SYSTEMIC EXAMINATION
Respiratory System:
Bilaterally equal breath sounds, No adventitious sounds
Cardiovascular System:
S1 & S2 Normal, No diastolic murmurs heard
Central Nervous System:
HMF – Normal
Per Abdomen:
Soft ,non tender , no organomegaly
11
LOCAL GENITAL EXAMINATION
 External Genitalia- Vulva normal, No clitoromegaly seen
 Per Speculum– Vagina and Cervix Healthy
 Per Vaginum – Cervix is Firm, Posterior, Mobile
Uterus Normal Size & Anteverted
Bilateral Adnexa Free
12
INVESTIGATIONS
Parameter Value
Hemoglobin 12gm%
Blood Sugar F/PP 98/115 mg/dl
Blood Group B Positive
HIV/ HBsAg/
AntiHCV/ VDRL
Negative
Mantoux test 3mm
13
HORMONE PROFILE
Parameter Values Normal Values
Follicular
Stimulating
Hormone (D2)
5.58 IU/L 3 – 10 IU/L
Luteinizing
Hormone(D2)
4.2 IU/L 1.9 – 12.5 IU/L
Serum Prolactin 15 μg/L <25μg/L
Serum Thyroid
Stimulating
Hormone
2.8 mIU/L 0.4 to 4.0 m IU/L
Anti–Mullerian
Hormone
8.4 ng/ml 1.5 – 4.0 ng/ml
Sr Testosterone 98 ng/dl 15- 70 ng/dl
14
ULTRASONOGRAPHY
Day-2 of
menses
Day-14
of
menses
15
HYSTEROSALPINGOGRAPHY
 Uterine Cavity Normal
 Bilateral Fallopian Tubes Visualized
 Bilateral Spill Seen
16
HYSTEROSCOPY
 Cervical Canal – Normal
 Endometrial Cavity – Normal
 Bilateral Ostia Visualised OSTIA
17
MALE PARTNER EVALUATION
18
CASE HISTORY
30 year old
Hindu
Graduate
Serving
Soldier(GD)
19
CASE HISTORY
No History Of-
Coital Difficulty
Premature Ejaculation
Erectile Dysfunction
Penile Discharge/Ulcerations
STD/ Infections of Genital Tract 20
PERSONAL HISTORY
No Alcohol No Smoking No Tobacco
Use
No Drug
Abuse
21
PAST HISTORY-
Viral Orchitis
Genital Trauma
Prior Pelvic or Inguinal Surgery
Prior Chemotherapy
Radiation
No History of-
22
FAMILY HISTORY
Birth defects
Cystic fibrosis
Reproductive failures
No History of-
23
GENERAL PHYSICAL EXAMINATION
Average built & nourished
Height- 172 cm Wt- 70 kg BMI-23.8kg/m2
Male pattern hair distribution
No gynaecomastia/ No Thyromegaly
No pallor/ icterus/ edema/ cyanosis/ clubbing/
lymphadenopathy
PR: 80bpm BP: 126/80 mm Hg RR- 20 bpm
24
SYSTEMIC EXAMINATION
Respiratory System:
Bilaterally equal breath sounds, No adventitious sounds
Cardiovascular System:
S1 & S2 Normal, No diastolic murmurs heard
Central Nervous System:
HMF – Normal
Per Abdomen:
Soft ,non tender , no organomegaly , Bilateral hernia sites normal
25
LOCAL GENITAL EXAMINATION
Penis and bilateral scrotal sacs
normal
Testis palpable in both
scrotum ,Normal volume
No
varicocele/Hydrocele
26
SEMEN ANALYSIS
No motile/immotile spermatozoa seen
27
INVESTIGATIONS
Semen Analysis- Azoospermia
Semen culture : Sterile
USG Scrotum- Normal Scan
28
INVESTIGATION
PARAMETER VALUES
HIV Non Reactive
HBsAg Negative
Anti HCV Negative
VDRL Negative
Blood Group A Positive
29
INVESTIGATIONS
Parameter Values Normal values
Testosterone 800 ng/dL 270- 1070 ng/dL
Follicle Stimulating
Hormone
4.8 IU/L 2- 12 IU/L
Luteinizing Hormone 3.2 IU/L 2-9 IU/L
Thyroid Stimulating
Hormone
2.8 m IU/L 0.5 – 4.15 m IU/L
30
DIAGNOSIS
PRIMARY INFERTILITY
Male factor: Obstructive Azoospermia( OA )
Female factor: Polycystic Ovarian Syndrome
31
DISCUSSION
32

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Azoospermia case

Editor's Notes

  1. Transvagical ultrasound is considered the gold standard in the diagnosis of polycystic ovaries. Features can affect either one (unilateral polycystic ovary) or both ovaries. Features include presence of 10-12 or more follicles 3,5 individual follicles are generally similar in size and measure 2-9 mm in diameter 7 peripheral distribution of follicles - this can give a "string of pearls" appearance background ovarian enlargement (volume greater than 10 cc 7) central stromal brightness +/- prominance General imaging differential considerations include multi-follicular ovaries (MFO) 2 -  fewer cysts ( ~ 6 or more per ovary), which tend to be larger (up to 10 mm in diameter) and distributed throughout the ovary with no stromal hypertrophy
  2. AMH is expressed by granulosa cells of the ovary . it is a measure of certain aspects of ovarian function,[19] useful in assessing conditions such as polycystic ovary syndrome and premature ovarian failure
  3. patient is truly azoospermic is confirmed by centrifugation of the semen sample with meticulous microscopic examination of the pellet on two different occasions. - The presence of any sperm in the pellet rules out bilateral ductal obstruction, and the patient should be evaluated for oligospermia and not azoospermia.