anthrax staining diagnosis

1. Mc’Fadyean Reaction
ABHIJITH SP
CVAS POOKODE

2. INTRODUCTION
• Used to identify Anthrax bacillus in microscope.
• Actually a Staining technique.
• Employed for the presumptive diagnosis of anthrax in animals.
• Stains the polypeptide capsular material of anthrax bacilli that is composed
of poly-d–Glutamic Acid.

3. PROCEDURE
• Take blood from tip of ear and make a impression slide.
• This is stained with Poly Chrome Methylene Blue (PCMB) for 30 seconds.
• Examine under microscope.
• If the sample is positive, an amorphous purplish material is noticed around
the blue bacilli.
• This purplish material is capsule around blue bacilli body.

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