brucellosis diagnosis agglutinin reaction field test easy test

1. ROSE BENGAL
PLATE TEST
ABHIJITH SP
CVAS POOKODE

2. Introduction
• Brucellosis is a worldwide zoonosis caused by the bacterial genus Brucella.
• These organisms localize in the reproductive organs of host animals, causing
abortions and sterility.
• They are shed in large numbers in the animal's urine, milk, and placental
fluid.
• Exposure to infected animals and animal products causes the disease in
humans.

3. Introduction
• Brucellosis was first diagnosed by Wright and Smith in 1897.
• Rose Bengal test detects antibodies.
• The synonyms of Brucellosis are:
• 1.Undulant fever
• 2.Malta fever.
• 3.Gibraltar fever.
• 4.Mediterranean fever

4. Rose Bengal Plate Test
• It is a rapid plate agglutination test by using a slide.
• Used in field for diagnosis of Human brucellosis and Bovine Brucellosis.
• It is actually a screening test and positive reaction samples should be
confirmed by STAT or CFT.
• Time taken for reaction is 2 minutes.
• Cheap and Faster.
• Synonyms: Rose Bengal Agglutinin Test, Rose Bengal Dye Test

5. Materials Required
• 1.Rose Bengal Dye stained antigen
• 2.Micropipette
• 3.Microscopic slides
• 4.Applicator sticks.
• 5.Gloves

7. Procedure
• A clean and sterilized slide is placed on a white background.
• Rose Bengal Dye stained Antigen vial should be gently swirled.
• With a micropipette two drops of antigen are taken on both ends of glass slide.
• An equal volume of test sera is added to the antigen.
• With a non-metallic stick, gently mix the antigen and sera.
• Rotate slide is slanted orbital motion.
• Rotate the slide with a mechanical rotator at 80-100 rpm for 2 minutes (if possible).
• Appearance of agglutinin clumps indicate positive reaction.
• Positive samples should be confirmed by STAT or CFT.

9. Result

11. Result and Interpretation
• No agglutination= absence of specific antibodies
• Agglutination (even slight) = presence of specific antibodies
• If agglutination appear after 15 seconds = (1:640)
• If agglutination appear after 30 seconds = (1:320)
• If agglutination appear after 1 min. = (1:160)
• If agglutination appear after 1.30 min. = (1:80)

12. THANK YOU