This document contains 25 multiple choice questions about protozoan parasites, their lifecycles, transmission, and diseases they cause in humans. The questions cover topics like Giardia lamblia, Entamoeba histolytica, their sites of infection in the human body, diseases associated with them like amebic colitis and hepatitis, modes of transmission, diagnostic tests and more.

1. Protozoa (E. Histolytica, trichomonas, giardia).
Q1. Onlyprotozoaparasite foundinLumen&human small intestine (Duodenum&Jejunum)
a) Giardia lamblia b) E. Histolytica c) E. Despar d) Giardia
Q2. Protozoatransmittedby sexual contactexcept.
a) Trichomonasvaginalis b) Giardialamblia c) E.Histolytica d) Toxoplasma
Q3. Flagellatedparasite are all except.
(a)Giardialambliab) Trichomonasc) Trypanosomad) leishmaniae) Toxoplasma
Q4. Causative Agentof PAM(primaryamoebicmeningoencephalitis).
a) Naegleriafowleri b) E.Histolytica c) Endolimax nana d) Dientamoebafragilis
Q5. Causative agentof granulomatous amoebicmeningoencephalitis.
a) Acahthamoeba
b) E. Histolytica
c) E. Despar
d) Naegleria
Q.6 whichof the followinginfectionleadstomalabsorption
a) Giardialamblia
b) Ascaris
c) Necatoramericanus.
d) Ancyclostomaduodenale
Q7. Recurrentgiardiasisisassociatedwith:-
a) SCID
b) Combinedvariableimmunodeficiency
c) Digeorge syndrome
d) C8 deficiency
e)
Q8.Diagnostictestforamoebichepatitisis
(a)Indirecthaemagglutination
(b) isolationfrompus
(c) Isolationfromwall of cavity
(d) Cystin stool
Q9.Cysticformof all are seeninmanexcept
(a)E.histolytica
(b)Giardia
(c)trichomonas
(d)Trichomonas
Q10.Amoebanotfoundinhuman intestine
(a)E.histolytica
(b) E.coli
(c)E.nana

2. (d)E.gingivalis
Q11.All are true about entamoebahistolyticaexcept
(a)Cystare 8 nucleated
(b) Cystare 4 nucleated
(c)Trophozoitescolonize incolon
(d)Chromatidbodiesstainediniodides
Q12.Investigationof choice foramoebiasisis
(a)ELISA
(b) E.coli
(c)H.nana
(d)E.gingivalis
Q13.Most commontestdone forinvasive amoebiasisis
(a)IHA indirecthaemagglutination
(b) microscopy
(c) Colonoscopy
(d) ELISA
Q14.Amoebalackcystic stage and transmittedbydirectoral contact
(a)E.gingivalis
(b) E.coli
(c)Acanthamoeba
(d)Naegleri
Q15.Infective stage of E.histolytica
(a)Trophozoite
(b)Quadrinucleatedcyst
(c)metacystictrophozoite
(d)Amoeboidform
Q16.True aboutamoebiccolitisexcept
(a) CausedbyE.histolytica
(b) Cystcontain8 nuclei
(c) flaskshapedulcerpresent
(d) Caecumismost commonlyeffected
Q17.A patientpresentwithlowerGIbleedingonsigmoidoscopyflaskshapedulcerseenmostlikely
organismis
(a)E.histolytica
(b)Giardia
(c)Toxoplasma

3. (d)Plasmodium
Q18.Analysisof stool onwetmountshowsmotile protozoawithoutRBC&pus cellsmostlikely
organismis
(a)B.coli
(b)Giardiasis
(c)T.hominis
(d)E.histolytica
Q19.Binucleate pearshape trophozoiteseenin
(a)Giardiasis
(b)E.histolytica
(c)Toxoplasma
(d)Trichomonas
Q20.Amoebiccysteine proteinase virulentfactorpresentin
(a)E.histolytica
(b)E.dispar
(c)E.gingivalis
(d)Tooplasma
Q21. Fallingleaf motilityseenin
(a)Giardia
(b)Trypanosoma
(c)leishmania
(d)Listeria
Q22.Contentof Amoebicabscessiscalledas
(a)Anchovy paste
(b)pasty
(c)Creamypus
(d)pus
Q23.flaskshape ulcerof amoebiasishave

4. (a)narrowneckand stoutbase
(b)longneckandbroadbase
(c)broadbase and broadneck
(d)narrowneckandbroad base
Q24.A patientpresentwithpaininrighthypochondrium, the movementof chestonrightside
diminished,apical impulse of heartdisplacedupwardandlaterallyondiagnosticUSGaspirate shows
anchovysauce appearance mostlikelyorganismcausedthisabscessis
(a)E.dispar
(b)Toxoplasma
(c)E.coli
(d)E.histolytica
Q25.Excystationof cyst of E.histolyticaoccursin
(a)Stomach
(b)duodenum
(c)jejunum
(d)caecum
1 A
2 D
3 E
4 A
5 A
6 A
7 B
8 A
9 D
10 D
11 A
12 B
13 A
14 A
15 B
16 B
17 A
18 B
19 A
20 A
21 A
22 A

5. 23 D
24 D
25 D
VIRUS (HEPATITIS, MISCELLANEOUS)
Q1. Most commonhepatitisassociatedwithbloodtransfusion
a) HepatitisA
b)HepatitisB
c)HepatitisD
d) HepatitisC
Q2. Most commonhepatitisvirustransmittedperinatally
a)HepatitisA
b)HepatitisB
c)HepatitisC
d)HepatitisD
Q3. Which of the followinghepatitisvirusiscultivable
a)HepatitisA
b)HepatitisB
c)HepatitisC
d)HepatitisD
Q4.Marker of acute HepatitisBis
a) HBV DNA polymerases
b) Ig G anti HBc
c) HBc Ag
d) Anti HBs Ag
Q5.Marker of Chronic Hepatitis B is
a)IgG anti HBc Ag
b)IgManti Hbc Ag
c)HBe Ag
d)Ig G anti HBc Ag + HBs Ag
Q6.Marker of Vaccination of hepatitis

6. a)Ig G anti HBc
b) HBe Ag
c) Anti HBs Ag alone
d)IgManti HBc Ag
Q7.Most Common Cause of fulminant Hepatitis in Pregnancy
a)Hepatitis B b) Hepatitis E c) Hepatitis A d)Hepatitis D
Q8.Which Hepatitis Virus is notorious for causing chronic hepatitis evolving cirrhosis
a)Hepatitis C b)Hepatitis B c) Hepatitis E d) cytomegalo virus
Q9.Hepatits A & Hepatitis E transmitted through
a) Blood route b)By inhalation c)Feco-oral d) All
Q10.Presence of HBeAg in patient with hepatitis indicates
a)Simple carrier b) Late convalescence c)Carrier status d)High infectivity
Q11.Hepatitis B is not transmitted by
a)Blood transfusion b)Pasteurized Albumin c)Cryoprecipitate d)Sexual contact
Q12.HBV is associated with all except
a)hepatitis cancer b) chronic Hepatits c)Hepatic adenoma d) Hepatitic Cirrhosis
Q13.Which of the following is not present in DANE particle
(a)Core Ag (b) Surface Ag (c) P53 (d)none
Q14.Commonest cause of acute hepatitis in India
(a) Hepatits A (b)Hepatitis B (c)Hepatitis C (d)Hepatitis B+ D
Q15.HBV variant present in India is
(a)Adw (b)Ayw (c)Adr (d)Ayw+Adr
Q16.A 48-year-oldfemale presentstoaphysicianwithmalaise,lossof appetite,nausea,moderate
fever,andjaundice.Laboratorytestsindicateamarkedincrease inserumtransaminases.Serology
for hepatitisvirusesisperformedand indicatespositiveresultsforthe presence of HBsAg,HBc IgM
antibody,andHCV antibody.AntibodytestsforHBsAb and HAV are negative.The resultsindicate:
(a) A dual infectionof HBV andHAV (b).ChronichepatitisA infection(c).Chronichepatitis B
infection (d)HepatitisCinfection(e).The presence of anacute HBV infection.
Q17.A patientpresentstoa physicianwithjaundice.Physical examinationrevealsanodular,
enlargedliver.CTof the abdomenshowsacirrhoticliverwithalarge mass. CT-guidedbiopsyof the
mass demonstratesamalignanttumorderivedfromhepaticparenchymal cells.Infectionwithwhich
of the followingviruseswouldmostlikelybe directlyrelatedtothe developmentof thistumor?

7. A. Epstein-Barrvirus(EBV) B.HepatitisB virus(HBV) C.Human herpesvirustype 8(HHV 8) D.
Human papillomavirus(HPV) E.Human T-lymphocyte virus(HTLV-1)
Q18.Prionare
(a)infectiousprotein(b)made upof bacteriaandvirusparticle (c)Nuclearmaterial (d)canbe cultured
incell free media
Q19.Which of the followingnotprionassociateddisease
(a)Scrapie (b)Kuru(c)Creutzfeldt-jakobdisease(d)Alzheimer`sdisease
Q20.Mad cow disease isdue to
(a)slowvirus(b)mycoplasma(c)bacteria(d)Fungus
Q21.Subacute sclerosingpanencephalitis isthe delayedmanifestationof
(a)measles(b)polio(c)mumps(d)influenza
Q22.Human cannabilismisassociatedwith
(a)Qfever(b)sleepingsickness(c)Trachoma(d)Kuru
Q23.Rota virusis detectedby
(a)Aginstool (b)Abinserum(c)Stool culture (d)Demonstrationof virus
4.Reassortmenttypicallyseenin
(a)parvovirus(b)rotavirus(c)hepadna(d)astrovirus
Q25.Human papillomaviruscontains
(a)SSRNA (b)DSDNA (c)SSDNA (d)DSRNA
Q26.Human papillomatosiscausedby
(a)HSV (b)HPV(c)HIV(d)HBV
Q27.Virusinducesaplasticcrisisinchildrensufferingfromsicle cell anaemia
(a)Parvo virus B19(b)Prvo virus B39(c)Parvo virus B9(d)Parvo virus B29
Q28.All are true about congenital rubella except
(a)Infection after 16wks of gestation result in major congenital defect
(b) Diagnosed when IgG Ab persist >6month
(c)Most common congenital defect are deafness, cardiac malformation,cataract
(d)Diagnosed when IgMAb at birth
Q29.full form of SARS is
(a)Severe acquired respiratory syndrome
(b)Severe acute respiratory syndrome
(c)Severe acquired rubella syndrome

8. (d)severe acute rhino sclerosis
Q30.Ebola virus belongs to family
(a)Filovridiae(b)coronaviridae(c)Arenaviridiae(d)flaviviridiae
Immunlogy( infection,immunity,Ag ,Ab,Ag-Ab reaction,complement system)
Q1.Haptenis
A) Same as isotope
B) Small molecularweightprotein
C) Requires carrierforspecificAbproduction
D) Simple haptensare precipitate
1 D
2 B
3 A
4 A
5 D
6 C
7 B
8 A
9 C
10 D
11 B
12 C
13 C
14 A
15 D
16 E
17 B
18 A
19 D
20 A
21 A
22 D
23 A
24 B
25 B
26 B
27 A
28 A
29 B
30 A

9. Q2.whichof the followingstatementistrue abouthapten
A) Itinducesbriskimmune response
B) It needscarrierto induce immune response
C) It isa T dependentantigen
D) It hasno associationwithMHC
Q3.All the followingstatementsaboutcarbohydrate Agare true except
A) It has lowerimmunogenicity
B) Memory response isseen
C) Cause polyclonal Bcell stimulation
D) Does notrequire stimulationbyTcells
Q4.whichof the followingTcell independentantigenactthrough
A) Tcell
B) B cell
C) Macrophages
D) CD8+ T cells
Q5. Which part of bacteriais mostantigenic
A) Proteincoat
B) Lipopolysachharide
C) Nucleicacid
D) Lipids
Q6. Antigencombiningsiteof antibody
A) Idiotype
B) Paratope
C) Epitope
D) Both a and b
Q7. A superantigenisbacterial productthat
A) Bindsto B7 andCD28 costimulatorymolecules
B) Bindsto the beteachainof TCR and Mhc class 2 of APCstimulatingTcell activation
C) Bindsto CD4+moleculescausing Tcell activation
D) It presentedbymacrophagestoa larger than normal numberof T helperCD4+ lymphocytes
Q8. Autoantigenis
A) Bloodgroupantigen
B) Forssmanantigen
C) Both
D) none
Q9. The mainof adjuvantisto increase

10. A) distribution
B) absorption
C) antigenicity
D) metabolism
Q10. All of the followingforcesare involvedinantigenantibodyreactionexcept
A) vanderwaal `s forces
B) electrostaticbond
C) hydrogenbond
D) covalentbond
Q11. Whichof the followingstatementistrue
A) Paul Bunnel testisusedtodiagnose measles
B) Rose Waalar testis a complementfixationstate
C) indirecthaemaagltinationtestislesssensitive thangel diffusiontest
D) antigenantibodyreactioncannotoccurinthe absence of electrolyte
Q12. The reactionbetweenantibodyandsolubleantigenisdemonstratedby
A) agglutination
B) precipitation
C) complementfixationtest
D) haemoagglutinationtest
Q13. Skintestbasedonneutralizationreactionis/are
A) casoni test
B) lepromintest
C) tuberculintest
D) shicktest
Q14. Naeglerreactionisatype of
A) neutralizationreaction
B) CFT
C) Precipitation
D) Agglutination
Q15. Coombstestis
A) Precipitationtest
B) Agglutinationtest
C) CFT
D) Neutralizationtest
Q16. Prozone phenomenonisseenwith
A) Same concentrationof antibodyandantigen
B) Is antigen excesstoantibody
C) Antibodyexcesstoantigen
D) Hyperimmune reaction

11. Q17.prozone phenomenonisafeature of
A) Tularaemia
B) Legionnaire`sdisease
C) Plague
D) Brucellosis
Q18.The followingmethodsof diagnosisutilize labelledantibodyexcept
A) ELISA
B) Hemagglutinationinhibitiontest
C) Radioimmunoassay
D) Immunofluorescence
Q19. Whichis notheterophile agglutinationtest
A) Weil felix test
B) Widal test
C) VDRl
D) All
Q20. Reactionof soluble antigenwithantibodyisknownas
A) Agglutination
B) Precipitation
C) Flocculation
D) Complementfixation
Q21.VDRl is a
A) Slide flocculationtest
B) Tube flocculationtest
C) Gel precipitationtest
D) Indirecthaemagglutinationtest
Q22. Isoantigensare
A) Foundinsome but all membersof species
B) Foundinsome but notall membersof species
C) Occurs indifferentbiological species,class andkingdom
D) All individual inparticularspecies
Q23. Antibodiesare mostresponsive to
A) recipienttissue
B) donortissue
C) isograft
D) autograft
Q24. Phagocytosisenhancedbycoatingthe surface of antigeniscalled
A) opsonisation
B) chemotaxis
C) decoding

12. D) CFT
Q25.DNA isdetectedby
A) Southernblot
B) Westernblot
C) Northernblot
D) Easternblot
Q26.most sensitivetestfordetectionof antigenis
A) RIA
B) ELISA
C) Immunofluorescence
D) Passive hemagglutination
Q27. A single immunoglobulinmoleculecontains
A) 1 lightchain,1 heavychain
B) 2 heavychains,1 lightchain
C) 2 lightchains,2 heavychains
D) 2 lightchains,1 heavychain
Q28. Whichof the followingclassspecificantigenicdeterminantsof animmunoglobulinisassociated
with
A) L-chain
B) H-chain
C) J-chain
D) Variable region
Q29.variable portion of antibodymolecule is
A) C-terminal
B) N-terminal
C) CHO moiety
D) None
Q30. Antigenbindingsite onantibodyis
A) Hinge region
B) Constantregion
C) Both D & E
D) Hypervariable region
E) Idiotype region
Q31. Antibodydiversityisdue toexcept
A) Gene rearrangement
B) Gene translocation
C) Antigenicvariation
D) Mutation
Q32. Portionof immunoglobulinmolecule withmolecularweightof 50000

13. A) Secretorytype
B) H-chain
C) L-chain
D) J-piece
Q33. The earliestimmunoglobulintobe synthesizedby the fetus is
A) Ig A
B) Ig M
C) Ig G
D) Ig D
Q34. Whichof the followingimmunoglobulinisresponsible foropsonisation
A) Ig A
B) Ig G
C) Ig E
D) Ig M
Q35. Activationof classical componentpathway
A) Ig A
B) Ig G
C) Ig M
D) Ig D
Q36. The secretorycomponentof immunoglobulinmoleculeis
A) Formedbyepithelial cellsof liningmucosa
B) Formedbyplasmacells
C) Formedbyepithelial cell andplasmacell
D) Secretedbybone marrow
Q37. Bronchial secretionsecretes
A) IgA
B) IgM
C) Ig D
D) Ig G
Q38. Pentamericstructure is
A) Ig M
B) Ig G
C) Ig D
D) Ig A
Q39. Whichprecipitate at50-60 0
C but disappearonheating
A) Heavychain
B) Lightchain
C) Both
D) None of these

14. Q40. Bence jonesproteinbestdescribedas
A) Chains
B) Gamma Chain
C) Kappa& lambdachain
D) Fibrinsplitproducts
Q41. All of the followingare partof innate immunityexcept
A) Complement
B) NKCells
C) Macrophages
D) T cells
Q42. All are true about innate immunityexcept
A) Nonspecific
B) Firstline of defence
C) Notaffectedbygenetic
D) Include complement
Q43. Active immunityisnotacquiredby
A) Infection
B) Vaccination
C) Immunoglobulintransfer
D) Sub-clinical infection
Q44.adaptive immunityisby
A) Infection
B) Injectionof antibodies
C) Injectionof lymphocytes
D) Immunization
Q45.type of immunityconferredonindividualbyvaccination
A) Artificial active
B) Artificial passive
C) Natural active
D) Natural passive
Q46.true aboutsecondaryimmune response
A) Both C & D
B) Usuallyof lowtitre
C) Antibodiesappearinshortterm
D) Persistforlong
Q47. Complementformedinliver
A) C2,C4
B) C3,C6,C9
C) C5,C8

15. D) C1
Q48. Whichof the followingbestdenotesclassicalcomplementpathwayactivationinimmune-
inflammatorycondition
A) C2,C3,C4 decreases
B) C2 C4 normal C3 decreases
C) C3 normal C2 C4 decreases
D) C2 C3 C 4 all are elevated
Q49. C3 convertasesactson
A) C4 2b
B) C4b 2B 3a
C) C4 b
D) C3
Q50. Centerof complementpathway
A) C3
B) C1
C) C5
D) C2
Q51. Chemoattractantis
A) C5a
B) C1
C) C3
D) C2
Q52. Opsoninsare
A) C3a
B) C3b
C) C5a
D) C5-C9
Q53. Hereditaryangioneuroticedemaisdue to
A) Deficiencyof C1inhibitor
B) Deficiencyof NADPHoxidase
C) Deficiencyof MPO
D) Deficiencyof properdin

16. 1 C
2 B
3 B
4 B
5 D
6 B
7 D
8 D
9 C
10 D
11 D
12 B
13 D
14 A
15 B
16 C
17 D
18 B
19 B
20 B
21 A
22 B
23 B
24 A
25 A
26 A
27 C
28 B
29 B
30 B
31 B
32 B
33 B
34 D
35 C
36 A
37 A
38 A
39 B
40 C
41 D
42 C
43 C
44 C

17. Bacteriology( haemophilus ,bordetella, brucella )
Q1. Brucellamelitensisiscommonlyfoundin(animal)
A) Pig
B) Sheep
C) Camel
D) Goat
E) Both B & D
Q2. Brucellosiscanbe transmittedbyall of the following,except
A) Contact withinfectedplacenta
B) Ingestionof rawvegetablesfrominfected forms
C) Persontopersontransmission
D) Inhalationof infecteddustoraerosol
Q3. Which of thisis capnophilic
A) Brucellaabortus
B) Brucellacanis
C) Brucellamelitensis.
D) Bordetellaspp.
Q4. For growingHemophilus spp.,whichmediumisbest?
a)Bloodagar.
b)Chocolate gar.
c)Nutrientagar.
d)Peptone water.
Q5. Standard agglutinationtetsisdone for….?
a. Hemophilus spp.
b. Brucellosis.
c. Salmonellosis.
d. Bordetella infection.
45 A
46 C
47 B
48 A
49 D
50 A
51 A
52 B
53 A

18. Q6. Regan-Lowe mediumisdone togrow:…..?
a. Bordetellaspp.
b. Brucellasuis.
c. Hemophilusaphrophilus.
d. H. ducreyi.
Q7. “School of fish”appearance isfoundin; …… ?
a. H. influenzae.
b. H. paraaphrophilus.
c. H. ducreyi.
d. S.aureus.
Q8. Urease positive motileBordetella spp.Is:…. ?
a. B. pertussis.
b. B. parapertussis.
c. B. bronchiseptica.
d. None of above.
Q9. 2- Mercaptoethanol isusedtodistinguishbetweenwhich2antibodiesformedinBrucellosis.?
a. IgMand IgG.
b. IgE and IgD.
c. IgY and IgA.
d. None of above.
Q10. Satelliitismaround S.aureus colonyonbloodagaris seenin.: …..?
a. H. influenzae.
b. H. parainfluenzae.
c. S. pneumoniae.
d. E. coli.
Q11. Whichof these isa speciesingenusBrucella?
a. B. neotomae.
b. B. burgdorferi.
c. B. pertussis.
d. B. aureus.
Q12. Bisectedpearl coloniesare seenin:….….?
a. Bordetella spp.
b. Brucella spp.
c. Sporothrix spp.
d. Hemophilus spp. St
Q13. Milkringtest isdone to detect:…
a. Brucellaspp.
b. Bordetellaspp.

19. c. S. aureus.
d. Enterococcus spp.
Q14. A farmerrearingsheep,present withcomplaintof feverandweaknessforlastone month
Withgeneralisedlymphadenopathyassociatedwithhepatomegaly,onliverbiopsynon-
caseatinggranulomaseen,mostlikelydue toinfectionwith..
a) Yersiniapestis
b) Brucellacanis
c) Francisellatularensis
d) Brucellamelitensis
Q15. Castanedamethodof bloodculture isemployedfor..?
a) Brucellaspp
b) Bordetellaspp
c) Francisellaspp
d) Enterococcus
Q16.Which of followingbacteriaisa commoncause of septicarthritisinchildren?
a. Brucellaspp.
b. Hemophilusinfluenzae.
c. S. pyogenes.
d. S. epidermidis.
Q17. Chancoidiscaused by;…. ?
a. H. aphrophilus.
b. H. ducreyi.
c. H. influenza.
d. T. pallidum.
Q18. Whichof followingdiseasesinvaccine preventable?
a. Brucellosis.
b. Hemophilus influenza type binfection.
c. B. pertussis infection.
d. Both b andc.
Q19. Lymphocytosispromotingfactorisalsoknownas:… ?
a.Pertussistoxin.
b. Choleratoxin.
c. Diphtheriatoxin.
d. Salmonella enterotoxin.
Q20. Whichof followingis/are virulence factorsof B.pertussis?
a. Filamentoushemagglutinin.
b. Tracheal cytotoxin.
c. Pertactin.
d. All of the above.
Q21. Whichof followingisacommon cause of sinusitis?
a. Bordetella sopp.
b. Pseudomonas spp.
c. H. influenzae.
d. Rotavirus.

20. Q22. Whichage groupis mostsusceptibletowhoopingcough?
a. 30-40 yrs..
b. Lessthan 2 years.
c. 2- 5 yrs.
d. More than 20 yrs.
Q23. Whichof followingdrugsisusedfortreatingbrucellosis?
a. Rifampicin.
b. Streptomycin.
c. Amikacin.
d. Eithera or b, withTetracycline.
Q24. Inwhichof the followingorganismcapsule notactedasvirulence factor
a) H.influenzae
b) Bordetella pertussis
c) N.meningitidis
d) Streptococcuspneumoniae
Q25.Malta feveriscausedby
a) Legionella
b) Borrelia burgdorferi
c) Brucella melitensis
d) pseudomonas
1 E
2 C
3 A
4 B
5 B
6 A
7 C
8 C
9 A
10 A
11 B
12 D
13 A
14 D
15 A
16 B
17 B
18 D
19 A
20 D
21 C
22 B
23 D
24 B
25 C
25