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Cell-biology
History of Cell biology
o Mid 1600s – Anton van Leeuwenhoek
• Improved microscope, observed many living cells
o Mid 1600s – Robert Hooke
• Observed many cells including cork cells
o 1850 – Rudolf Virchow
• Proposed that all cells come from existing cells
The Cell Theory
Fundamental idea of modern cell biology that
includes these three principles:
o All living organisms are composed of one or more cells
o Cells are the basic unit of structure and organization of
all living organisms
o Cells arise only from previously existing cells, with
cells passing copies of their genetic material on to their
daughter cells
4
-Nucleus -Cytoplasm
-Nucleolus -Cytoskeleton
-Cell membrane -Vacuole
-Mitochondria -Chloroplast
-Golgi Apparatus -Centrioles
-Lysosomes -Ribosomes
-Endoplasmic reticulum
Cell organelles
Nucleus
The nuclear envelope
Components:
Two cellular membranes
Barrier to ions, solutes,
macromolecules
Membranes fused to form pores
Complex assemblies of proteins
Outer membrane
Ribosomes
Continuous with RER
Nuclear pore
o The pore is 100 nm in total diameter.
o The nucleus of a typical mammalian cell has about 3000
to 4000 pores throughout its envelope
o Proteins synthesized in the cytoplasm cross the nuclear
envelop to initiate replication and transcription of genetic
material
o Similarly, mRNA, tRNA and ribosomal subunits built in
the nucleus cross through the nuclear pores to the
cytoplasm
o The nucleoplasm is a highly viscous liquid that surrounds
the chromosomes and nucleoli
o Many substances such as nucleotides (necessary for
purposes such as the replication of DNA) and enzymes
(which direct activities that take place in the nucleus) are
dissolved in the nucleoplasm
Nucleoplasm
Nucleolus
 The prominent structure in the nucleus is the nucleolus
 The nucleolus produces ribosomes, which move out of the
nucleus and take positions on the rough endoplasmic
reticulum where they are critical in protein synthesis
o Chromatin is the complex of DNA and protein (Histones)
that makes up chromosomes
o Each unreplicated chromosome contains a single
continuous DNA molecule
o Types of chromatin
a) Euchromatin- is a lightly packed form of chromatin, rich in
gene concentration, and is often under active transcription.
b) Heterochromatin- is a tightly packed form of DNA which
is inactive form of chromatin.
Chromatin

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Nucleus

  • 2. History of Cell biology o Mid 1600s – Anton van Leeuwenhoek • Improved microscope, observed many living cells o Mid 1600s – Robert Hooke • Observed many cells including cork cells o 1850 – Rudolf Virchow • Proposed that all cells come from existing cells
  • 3. The Cell Theory Fundamental idea of modern cell biology that includes these three principles: o All living organisms are composed of one or more cells o Cells are the basic unit of structure and organization of all living organisms o Cells arise only from previously existing cells, with cells passing copies of their genetic material on to their daughter cells
  • 4. 4 -Nucleus -Cytoplasm -Nucleolus -Cytoskeleton -Cell membrane -Vacuole -Mitochondria -Chloroplast -Golgi Apparatus -Centrioles -Lysosomes -Ribosomes -Endoplasmic reticulum Cell organelles
  • 6. The nuclear envelope Components: Two cellular membranes Barrier to ions, solutes, macromolecules Membranes fused to form pores Complex assemblies of proteins Outer membrane Ribosomes Continuous with RER
  • 7. Nuclear pore o The pore is 100 nm in total diameter. o The nucleus of a typical mammalian cell has about 3000 to 4000 pores throughout its envelope o Proteins synthesized in the cytoplasm cross the nuclear envelop to initiate replication and transcription of genetic material o Similarly, mRNA, tRNA and ribosomal subunits built in the nucleus cross through the nuclear pores to the cytoplasm
  • 8. o The nucleoplasm is a highly viscous liquid that surrounds the chromosomes and nucleoli o Many substances such as nucleotides (necessary for purposes such as the replication of DNA) and enzymes (which direct activities that take place in the nucleus) are dissolved in the nucleoplasm Nucleoplasm
  • 9. Nucleolus  The prominent structure in the nucleus is the nucleolus  The nucleolus produces ribosomes, which move out of the nucleus and take positions on the rough endoplasmic reticulum where they are critical in protein synthesis
  • 10. o Chromatin is the complex of DNA and protein (Histones) that makes up chromosomes o Each unreplicated chromosome contains a single continuous DNA molecule o Types of chromatin a) Euchromatin- is a lightly packed form of chromatin, rich in gene concentration, and is often under active transcription. b) Heterochromatin- is a tightly packed form of DNA which is inactive form of chromatin. Chromatin