Instrumentation of UV- Visible Spectroscopy. pptx

1. A
SEMINAR ON
Topic: Instrumentation of UV- Visible Spectroscopy
Presented By:
Hariom Jaiswal
M. Pharm (Pharmaceutics)
1st Year / 1st Sem
(2021-22)
INSTTITUTE OF TECHNOLOGY OF MANAGEMENT
GIDA, GORAKHAPUR, U.P

2. CONTENTS:
1. INTRODUCTION
2. INSTRUEMENTION
3. APLLICATION

3. INTRODUCTION OF UV- Visible
Spectroscopy
Ultraviolet and visible spectroscopy is the
measurement of the attenuation of the beam of
light after it passes through a sample or after
reflection form a sample surface absorption
measurement can be at single wave length or over
an extended spectral range.

4. INSTRUMENTATION OF UV
SPECTOSCOPY
Instrumentations for measuring the absorption of UV
or visible radiation are made up the following
components.
1. Light Sources.
2. Filter or Monochromator.
3. Sample containers or Sample cells.
4. Detector.

6. LIGHT SOURCE:
The best source of light is the one which is more
stable more intense and which gives range of
spectorism form 180 to 360 nm.
The most commonly used source as Hydrogen or
Deuterism lamp. The Xenon discharge lamps and
mercury.
Various type of radiation source are used.
• Hydrogen discharge lamp.
• Deuterism lamp.
• Xenon discharge lamp.

7. Filter or Mono-chromometer:
It is a device used to isolate the radiation of the
wavelength from wavelength of the continuous
spectra.
These are types of monochromatic devices.
1. Filter Paper.
2. Purism.
3. Gratings.
It is device are used to isolated the radation of desired
wavelength.

8. SAMPLE CONTANIER OR SAMPLE
CELLS :
The cells or cuvettes are used for handling liquid
samples.
The cells may either be rectangular or cylindrical in
nature and cleaning is carried out washing with
distilled water or with dilute alcohol, acetone.
The cell may either by cylindercal or rectanguler in
nature.

9. DETECTOR:
Device which converts light energy into electrical
signals, that are displayed on readout devices.
Types of detectors:
1- Barrier layer cell/photovoltaic cell.
2-Phototubes/photo emissive cell.
3-Photomultiplier tube.

10. APPLICATION OF UV- SPECTROSCOPY:
 Qualitative and Quantitative analysis.
 Detection of Impurities.
 Structure of elucidation of Organic compound.
 Structural analysis of Organic compound.
a. Effect of conjugation.
b. Effect of geometric isomerism.