1. What do you think the very first living microorganism used for nutrition (carbon, energy, nitrogen, etc)? 2. What is the advantage of being a facultative anaerobe vs. an aerotolerant anaerobe? Why do you think Streptococci are aerotolerant anaerobes but Escherichia coli are facultative anaerobes? 3. Can you think of a reason that bacteria might need to use active transport to move small molecules OUT of themselves? 4. How would you use an enrichment and isolation experiment to find new previously undiscovered biochemical reactions? .