1. What are the major differences between ELISA and ELISpot 2. The ELISpot assay can assist you in determining the type of adaptive T cell response involved during a particular infection or disease state. How can you use the ELISpot assay to measures specific T cell responses to HIV infection? Think about the unique cytokines released by specific cell types 3. EUSA assays typically use a standard curve. Does the ELISpot assay? why/why not? 4. How would you interpret results of an ELISpot in 1 in which in sample A you found 500 tiny little spots, versus sample B in which you found 100 big spots (about 4 times bigger that the size of sample A)? .