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1. The invasive spotted lanternfly has made it to Vermont. It feeds on apples and maple trees and
deposits honeydew that attracts other pests and encourages mold growth. The Vermont Agency
of Agriculture is therefore very concerned. As a scientist working for the US Department of
Agriculture, you're working to better understand the genetic bases of traits in this emerging crop
pest species. You've identified three important recessive traits, cold tolerance ( t ), super sweet
honeydew ( h ) , and rapid development (d) that could affect their ability to colonize Vermont.
Limited cold tolerance ( T ) , regular honeydew ( H ) , and normal development ( D ) are
dominant. You know these traits are linked to some degree based on your observations of
inheritance patterns. You want to map these traits to the chromosome and determine the genetic
distance between the loci. You cross purebreeding spotted lanternflies with cold tolerance, super
sweet honeydew, and rapid development with purebreeding spotted lanternflies with limited cold
tolerance, regular honeydew, and normal development. You then testcross the resulting F1
progeny and observe the following 1000 F2 progeny. A) Determine the gene order and fill in the
progeny genotypes in the table above using the gene annotations noted above. (4 pts) Draw a
genetic map indicating the gene order and genetic distance between all 3 gene pairs in units of
map distance (or cM ); show your work for full credit. Note: You can calculate distance of outer
loci by summing. ( 9 pts) B) Determine if the observed frequency of double crossovers is
consistent with the expected frequency by calculating the interference value (I). Interpret the
results as the % fewer double recombinants than expected. (4 pts)

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  • 1. 1. The invasive spotted lanternfly has made it to Vermont. It feeds on apples and maple trees and deposits honeydew that attracts other pests and encourages mold growth. The Vermont Agency of Agriculture is therefore very concerned. As a scientist working for the US Department of Agriculture, you're working to better understand the genetic bases of traits in this emerging crop pest species. You've identified three important recessive traits, cold tolerance ( t ), super sweet honeydew ( h ) , and rapid development (d) that could affect their ability to colonize Vermont. Limited cold tolerance ( T ) , regular honeydew ( H ) , and normal development ( D ) are dominant. You know these traits are linked to some degree based on your observations of inheritance patterns. You want to map these traits to the chromosome and determine the genetic distance between the loci. You cross purebreeding spotted lanternflies with cold tolerance, super sweet honeydew, and rapid development with purebreeding spotted lanternflies with limited cold tolerance, regular honeydew, and normal development. You then testcross the resulting F1 progeny and observe the following 1000 F2 progeny. A) Determine the gene order and fill in the progeny genotypes in the table above using the gene annotations noted above. (4 pts) Draw a genetic map indicating the gene order and genetic distance between all 3 gene pairs in units of map distance (or cM ); show your work for full credit. Note: You can calculate distance of outer loci by summing. ( 9 pts) B) Determine if the observed frequency of double crossovers is consistent with the expected frequency by calculating the interference value (I). Interpret the results as the % fewer double recombinants than expected. (4 pts)