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1 of 77
-               2412
             Friendrech Mieseher)


-                 2453 Albrecht Rossel


                                         2
        1.            pyrimidine)
             2.       purine)
-         2492         Erwin Chargaff)
                         DNA
                    A=T,C=G


-          2493 – 2495      (M.H.F. Wilkins)


DNA                             DNA
          (helix)         polynucleotide
      1
JAMES D.WATSON     FRANCIS H.C. CRICK
                          DNA

1.         DNA
2.
3.
                      (DEOXYRIBOSE)
4.
5.
      DNA
      ADENINE (A)       THYMINE (TP) (       2
        CYTOSINE(C)        GUANIN (G) (
-     1


       -
                                           UAA, UGA

mRNA                                 Terminating Codo
    -          mRNA
            Codon
       -                      tRNA
tRNA       mRNA        tRNA
                           mRNA         tRNA     ant
           codon GCA     mRNA
Triplet Code
3 Nucleotides

    Codon

  Amino acid
DNA replication)


semiconservative replication)




                                         DNA
1.   helicase)
                 replication fork)
SSB (Single Strand Binding protein)
RNA   RNA primer)
RNA primase)          DNA
deoxy
      -

DNA       leading strand)
DNA            leading
                                     DNA
   -

  origin of replication)
                             Okazaki fragment)
lagging strand)
DNA
-                   -   exonuclease)

    ligase)              DNA
genetic information)
                RNA (single - stranded)
                       (copy) RNA       gene
RNA Synthesis
                          base pairs
(structural gene / gene proper)    DNA
** Transcription
    Non - sense strand
                            base     RN
          Non-template strand    Sense s
1.DNA template
    Promoter
    Structural gene(
    Terminator
2. RNApolymerase
3.Ribonucleotides
    Adenine triphosphate(ATP)
    Guanine triphosphate(GTP)
    Uridine triphosphate(UTP)
    Cytosine triphosphate (CTP)
**DNA           Gene        DNA


Promoter……gene proper....... Terminator
          (Coding region)
Transcription

    InitiationInitiation
2.2.Elongation
3.3.Termination
anscription 3 steps

    Initiation
   Elongation
  Termination
-InitiationInitiation     Trans
** RNA polymerase        promoter
      promoter :
    * RNA polymerase             DNA
                    --          Coding s
      RNA polymerase      ribonucleotide
                           Start point
– Elongation :
** RNA polymerase    ribonucleotides
        RNA                        --
          ribonucelotides
     RNA            DNA
           RNA
– Termination :
             DNA             gene
    Terminator
                    terminator
    Rho - independent termination
RNA                       hairpin)
                  RNA polymerase -->
2.2.Rho - dependent termination
   RhoRhofactor factor )
           terminator
mRNA




             tRNA
       AUG          ’
tRNA
                mRNA




’      ’ tRNA
tRNA
       mRNA
U
UGA


      tRNA
DNA Replication Transcription

             S phase             Protein
                                synthesis
   DNA

                 TAGC           UAGC
RNA primer
             DNA polymerase        RNA
                               polymerase
                  ’    3’        5’   3’
-                       Friedrich Miescher



-
-
                              chromosome
theory of inheritance
-                         R. Feulgen
                             Fuchsin

-
Walter S. Sutton )

                          -

                  Walter S. Sutton )

                       Walter Sutton)

chromosome theory of inheritance)
2
autosome   sex chromosome



     1.


     2.
1.

        1.1     XO

                                     21

                     20 + XO
                      2           10 + X
     10 + O

                     (10 + X)
                X
                       (10 + O)
     autosome
1.2   XY




AA + XY
                        2         A+X
       A+Y
                   X         Y
                                   AA
+ XX
                            A+X
1.3        ZW



        XY
                   XY
                              AA + ZZ

  A+Z
                                   AA
+ ZW
                    2        A+Z
-            . . 1922
                           autosome
                            X

       gene (genetic balance)     gene
              2          male tendency
gene                autosome      femal
tendency gene                          X

                autosome
X
                        autosome / X
chromosome = 1
2.

                  diploid , 2n
= 32
               haploid , n =
16
              meiosis


= 16
                        meiosis
       = 16

         32
2
1.
                                    2

1.1

     1
                       47
         45        1            2

              XX

              XY
1)       Down's syndrome)


     -

     -
     -
     -
     -
Edward's syndrome)


        -

        -
        -
        -
-   -
Cri-du-chat
syndrome)


-
-
-


                             Cat-
cry-syndrome
X                Y



                          X

    X
           X

                         XO

Turner's syndrome)
X

                    XXX
XXXX

              XXX      XXXX
                      Super
female)
                    XXY
XXXY

              XXY         XXXY
Y
                   Y
     Y
         XYY

    XYY
      Super men)

-
-
-
-
-
Mutation

                        mutation




                       chromosome
mutation)
             gene mutation         point
mutation)
1.               chromosome
     mutation)




1)

2)
2.                     gene mutation
     point mutation)
radiation)

Ionizing Radiation

Non-Ionizing Radiation

                     colchicine)


               dichlovos)
             paraquat)
Somatic
cell)



        Sex cell)
Genetic
    Engineering)
              genetic engineering)




GMOs(genetically modified organisms)
GMO

                     DNA

      GENE)
                      GMO

      GMO

      GMO

GMO
               GMO
2.        1.


2.1            Agro-Bacterium



2.2   GENE GUN)

DNA
                      DNA
,
,


2539
GMO

               GMO)
-
-



-
    delay ripening)
-
-   (Potato)

               Bacillus thuringiensis
GMO

2.
3.
4.
5.
6.
7.
GMOs

1.           GMOs

2.
                       DNA
                GMOs

3.           GMOs

4.
 allergen)
6.
10.
1.         plasmid

2.

3.   PCR
1.


2.
oocyte
metaphase II
                                  (recipient
cell)




                 Wilmut
1997
               (dolly)
1.

2.

3.
4.

5.
ยีน โครโมโซม และพันธุศาสตร์วิศวกรรม

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ยีน โครโมโซม และพันธุศาสตร์วิศวกรรม

  • 1.
  • 2.
  • 3. - 2412 Friendrech Mieseher) - 2453 Albrecht Rossel 2 1. pyrimidine) 2. purine)
  • 4. - 2492 Erwin Chargaff) DNA A=T,C=G - 2493 – 2495 (M.H.F. Wilkins) DNA DNA (helix) polynucleotide 1
  • 5. JAMES D.WATSON FRANCIS H.C. CRICK DNA 1. DNA 2. 3. (DEOXYRIBOSE) 4. 5. DNA ADENINE (A) THYMINE (TP) ( 2 CYTOSINE(C) GUANIN (G) (
  • 6.
  • 7.
  • 8. - 1 - UAA, UGA mRNA Terminating Codo - mRNA Codon - tRNA tRNA mRNA tRNA mRNA tRNA ant codon GCA mRNA
  • 9.
  • 10.
  • 11. Triplet Code 3 Nucleotides Codon Amino acid
  • 13. 1. helicase) replication fork)
  • 14. SSB (Single Strand Binding protein)
  • 15. RNA RNA primer) RNA primase) DNA
  • 16. deoxy - DNA leading strand)
  • 17. DNA leading DNA - origin of replication) Okazaki fragment) lagging strand)
  • 18. DNA - - exonuclease) ligase) DNA
  • 19. genetic information) RNA (single - stranded) (copy) RNA gene RNA Synthesis base pairs (structural gene / gene proper) DNA
  • 20.
  • 21. ** Transcription Non - sense strand base RN Non-template strand Sense s
  • 22. 1.DNA template Promoter Structural gene( Terminator 2. RNApolymerase 3.Ribonucleotides Adenine triphosphate(ATP) Guanine triphosphate(GTP) Uridine triphosphate(UTP) Cytosine triphosphate (CTP)
  • 23. **DNA Gene DNA Promoter……gene proper....... Terminator (Coding region)
  • 24. Transcription InitiationInitiation 2.2.Elongation 3.3.Termination
  • 25. anscription 3 steps Initiation Elongation Termination
  • 26. -InitiationInitiation Trans ** RNA polymerase promoter promoter : * RNA polymerase DNA -- Coding s RNA polymerase ribonucleotide Start point
  • 27. – Elongation : ** RNA polymerase ribonucleotides RNA -- ribonucelotides RNA DNA RNA
  • 28. – Termination : DNA gene Terminator terminator Rho - independent termination RNA hairpin) RNA polymerase --> 2.2.Rho - dependent termination RhoRhofactor factor ) terminator
  • 29.
  • 30. mRNA tRNA AUG ’
  • 31. tRNA mRNA ’ ’ tRNA
  • 32. tRNA mRNA
  • 33. U UGA tRNA
  • 34. DNA Replication Transcription S phase Protein synthesis DNA TAGC UAGC RNA primer DNA polymerase RNA polymerase ’ 3’ 5’ 3’
  • 35.
  • 36.
  • 37. - Friedrich Miescher - - chromosome theory of inheritance - R. Feulgen Fuchsin -
  • 38. Walter S. Sutton ) - Walter S. Sutton ) Walter Sutton) chromosome theory of inheritance)
  • 39.
  • 40.
  • 41.
  • 42. 2 autosome sex chromosome 1. 2.
  • 43. 1. 1.1 XO 21 20 + XO 2 10 + X 10 + O (10 + X) X (10 + O) autosome
  • 44. 1.2 XY AA + XY 2 A+X A+Y X Y AA + XX A+X
  • 45. 1.3 ZW XY XY AA + ZZ A+Z AA + ZW 2 A+Z
  • 46. - . . 1922 autosome X gene (genetic balance) gene 2 male tendency gene autosome femal tendency gene X autosome X autosome / X chromosome = 1
  • 47. 2. diploid , 2n = 32 haploid , n = 16 meiosis = 16 meiosis = 16 32
  • 48.
  • 49. 2 1. 2 1.1 1 47 45 1 2 XX XY
  • 50. 1) Down's syndrome) - - - - -
  • 51. Edward's syndrome) - - - - - -
  • 52. Cri-du-chat syndrome) - - - Cat- cry-syndrome
  • 53. X Y X X X XO Turner's syndrome)
  • 54. X XXX XXXX XXX XXXX Super female) XXY XXXY XXY XXXY
  • 55. Y Y Y XYY XYY Super men) - - - - -
  • 56.
  • 57. Mutation mutation chromosome mutation) gene mutation point mutation)
  • 58. 1. chromosome mutation) 1) 2)
  • 59. 2. gene mutation point mutation)
  • 60. radiation) Ionizing Radiation Non-Ionizing Radiation colchicine) dichlovos) paraquat)
  • 61. Somatic cell) Sex cell)
  • 62. Genetic Engineering) genetic engineering) GMOs(genetically modified organisms)
  • 63. GMO DNA GENE) GMO GMO GMO GMO GMO
  • 64. 2. 1. 2.1 Agro-Bacterium 2.2 GENE GUN) DNA DNA
  • 65. ,
  • 67. GMO GMO) - - - delay ripening) -
  • 68. - (Potato) Bacillus thuringiensis
  • 70. GMOs 1. GMOs 2. DNA GMOs 3. GMOs 4. allergen) 6. 10.
  • 71.
  • 72. 1. plasmid 2. 3. PCR
  • 73. 1. 2.
  • 74. oocyte metaphase II (recipient cell) Wilmut 1997 (dolly)
  • 75.