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PLANT GROWTH AND
DEVELOPMENT- CLASS XI
ISC
1
PLANT
GROWTH
PLANT
GROWTH
INDEFINITE
Roots,
stems and
branches
DEFINITE
Leaves,
flowers
and fruits
2
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3
 The first step to the process of plant
growth is seed germination
 It is one of the most fundamental and
conspicuous characteristics of living
organisms
 It is the net result of various processes
that together cause an irreversible
permanent increase in the size of of
an organ or its parts
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4
 Growth is accompanied by anabolism
and catabolism that occur in the body
at the expense of energy
 It is intrinsic or internal
 It is generally indeterminate
 This is due to meristems
 Cells derived from meristems grow in
size, differentiate and form the bulk of
the plant body
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5
 Growth by meristematic activity is
called open form of growth
 Apical shoot, root meristems and
intercalary meristems cause primary
growth
 In dicotyledons and gymnosperms the
lateral meristems, vascular cambium
and cork cambium appear later in life
but are indeterminate causing
secondary growth
MERISTEMS
INDETERMINATE MERISTEMS DETERMINATE MERISTEMS
 Root apical
meristem, shoot apical
meristem, and
lateral meristem are
active
throughout the life of
the
plant and are
thus
indeterminate
meristems
 The intercalary
meristem
6
present in leaf and
flower
primordia
ha
s
a
shor
t
period of activity and
is
consumed in the
formation of organs and
is thus of the
determinate type
7
UNLIMITED GROWTH IN A WOODY PERENNIAL
8
Meristematic
or formative
Elongation
or
enlargement
Maturation or
differentiation
9
PHASES OF PLANT GROWTH
FORMATIVE
PHASE
10
 Phase of cell formation or division
 Occurs at the root apex,shoot apex
and other regions with meristematic
tissues
 New cells are produced by Mitosis
 Rate of cellular respiration is very high
 Some daughter cells retain the capacity
to divide while the others enter the next
growth phase
 Due to the increase in the cell number
the areas where divisions occur show
some growth
PHASE OF ELONGATION OR
ENLARGEMENT
11
 Cells formed in the formative phase enlarge
 Cell walls grow by deposition of new cell
wall materials
 A central vacuole appears
 At this stage some protoplasm is added
 In isodiametric cells there is significant growth
axially so that roots and stems elongate,
though they enlarge in all directions
 This phase corresponds to the exponential
phase
 Rate of respiration is high but lesser than in
the previous phase
PHASE OF
DIFFERENTIATION
12
 Cell wall grows in thickness and
gradually undergoes structural and
physiological changes as per its
location
 Formation of root hair, elongation of
xylem and phloem fibres, guard cells
of
stomatal
epidermi
s,
apparatus, cutinisation of
chloroplasts in
mesophyll,
suberisation of cork cell etc are
examples of structural differentiation
…continue
d
 Root hair take up the function of
absorption, mesophyll cells
photosynthesise, xylem and phloem
bring about ascent of sap and
translocation of food respectively
 Mature cells do not grow further after
differentiation
 NOTE: THE TIME PERIOD FROM THE
MERISTEMATIC PHASE TILL THE
MATURATION PHASE IS THE GRAND
PERIOD OF GROWTH
13
MEASUREMENT OF
GROWTH
 Growth in plants is measured in terms
of:
Increase in the number of cells produced
Increase in the girth and length of
shoot and root
Increase in the dry weight of organ and
increase
in the area of leaf or volume of a fruit
14
MEASUREMENT OF GROWTH
IN TERMS OF LENGTH
AUXANOMETER
ARCAUXANOMETER PFEFFER’SAUXANOMETER
HORIZONTAL
MICROSCOPE
FIXED AT A CLOSE DISTANCE TO THE
GROWING APEX AND AFTER A LAPSE OF
TIME MOVED UP
DISTANCE OF UPWARD MOVEMENT= GROWTH
DIRECT
METHOD
15
WITH THE HELP OFA SCALE
TO FIND CHANGE IN LENGTH AFTER
PARTICULAR TIME INTERVALS
HORIZONTAL MICROSCOPE
16
ARC
AUXANOMETER
17
 Consists of a curved graduated scale,
a pulley with a groove and a pointer
which moves on a scale
 One end of a thread is tied to the stem
tip of a potted plant and then hung on
the pulley of the auxanometer
 The other end is tied of the thread is
tied to a small weight to keep the
thread stretched
18
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19
 The pulley is fixed with a long
aluminium pointer which slides over a
graduated arc
 As the stem grows in length the
weight pulls the thread down
 The pointer moves on the graduated
arc indicating growth
Figure 1 An auxanometer is a device for making automated measurements of growth. A figure of a late 19th century auxanometer
taken from Wilhelm Pfeffer's classic textbook is shown [<ce:cross-ref refid="bib0005"> 1</ce:cross-ref> ].
20
Edgar P Spalding , Nathan D
Miller
Current Opinion in Plant Biology Volume 16, Issue 1 2013 100 -
104
http://dx.doi.org/10.1016/j.pbi.2013.01.001
PFEFFER’SAUXANOMETER
GROWTH
RATES
 Growth in unit time is growth
rate
GROWTH
RATES
ARITHMETI
C
Lt = L0 + rt
GEOMETRI
C
W1 =W0ert
21
Arithmetic
Growth
22
 Growth is constant from the beginning
 Growth progresses arithmetically
e.g. 2to4to6to 8to10
 Found in root and shoot apices
 Single meristematic cell divides into
two of which one continues to divide
while the other differentiates and
matures
 Lt = L0 + rt
Lt = Length after time t
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23
L0 = length at the
beginning
 r = growth rate or elongation per unit time
LINEAR CURVE OF ARITHMETIC GROWTH
24
GEOMETRIC
GROWTH
25
 All daughter cells of the mother cell
divide
 Common in unicellular organisms and
microorganisms
 The initial growth is slow (lag phase),
and it increases rapidly thereafter at
an exponential rate (exponential
phase).
 With limited nutrient supply, the growth
slows down after sometime (stationary
phase) or it may stop
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26
 A typical S-shaped or sigmoid growth
curve is obtained in organisms living in
a natural environment
 It is not uniform in plants showing
seasonal activity showing small
steps indicating
stoppage and
resumption of
growth every year
 W1 =W0ert
 W0 = initial size at the
beginning
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27
 W1 = final size(weight,
height
,
number)
 r = growth rate
 t= time for which the growth continues
 e = base of natural logarithms
 The magnitude of growth rate is called
efficiency index by Blackman(1919) as
organisms with higher growth rates will
outperform others
GEOMETRIC GROWTH
28
C = stages during embryonic
development showing both the
types of growth rates
29
Absolute growth
rate
30
 It is the measurement and comparison
in total growth of two organs or
organisms per unit time
 A bell shaped curve is obtained during
the grand period of growth
 Initial rise is lag and log phases
 The top Gmax
 Declining curve = declining growth
BELL SHAPED ABSOLUTE GROWTH RATECURVE
31
Relative Growth
Rate
32
 Growth rate per unit time shown as
percentage of initial size
 RGR = Growth in a given time period
*100
Initial size
 In the next slide AGR is same in both the leaves
while RGR of leaf A is faster due to its smaller
size
Diagrammatic comparison of absolute and
relative growth rates. Both leaves A and B
have increased their area by 5 sq.cm in a
given time to produce A’,B’leaves.
33
FACTORS AFFECTING PLANT
GROWTH
 SUPPLY OF NUTRIENTS: Nutrients
(macro and micro essential elements) are
required by plants for the synthesis of
protoplasm and act as source of energy
 WATER: Turgidity of cells helps in
extension growth. Thus, plant growth and
further development is intimately linked to
the water status of the plant. Water also
provides the medium for enzymatic
activities needed for growth
34
…continue
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35
in
releasin
g for
growth
 OXYGEN: Oxygen helps
metabolic energy
essential activities
 SUITABLE TEMPERATURE: 28°C to
30°C is optimum for plant growth. Low
temperature inactivates enzymes and
a temperature above 45°C hinders
growth
protoplasm
an
d
by denaturing
enzymes,
causin
g
coagulati
ng
excessi
ve
transpiratio
n
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36
 C:N RATIO: Regulates specific growth
patterns
 For example, presence of more N and
less Carbohydrates synthesises more
protoplasm and less mechanical tissues
with vigorous mechanical growth
 Leaves of such plants are succulent,
large and dark green
 If C:N ratio is larger, then more cell wall
materials and mechanical tissues are
synthesised
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37
intensity, duration
and direct
effect on
plant
 LIGHT: light
quality have a
growth
 Young plants get etiolated in absence
of light
 Environmental signals such as light
and gravity also affect certain
phases/stages of growth.
DIFFERENTIATION,
DEDIFFERENTIATION AND
REDIFFERENTIATION
38
 The cells derived from root apical and shoot-
apical meristems and cambium differentiate
and mature to perform specific functions
 This act leading to maturation is termed as
differentiation
 During differentiation, cells undergo few to
major structural changes both in their cell
walls and protoplasm. For example, to form a
tracheary element, the cells would lose their
protoplasm. They also develops a very
strong, elastic, lignocellulosic secondary cell
walls, to carry water to long distances even
under extreme tension
…continue
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39
 Plants show another interesting phenomenon.
 The living differentiated cells, that by now
have lost the capacity to divide can regain
the capacity of division under certain
conditions. This phenomenon is termed as
dedifferentiation
 For example, formation of meristems –
interfascicular cambium and cork cambium
from fully differentiated parenchyma cells
 While doing so, such meristems/tissues are
able to divide and produce cells that once
again lose the capacity to divide but mature
to perform specific functions, i.e., get
redifferentiated
DEVELOPME
NT
 Development is a term that includes
all changes that an organism goes
through during its life cycle from
germination of the seed to
senescence
 Diagrammatic representation of the
sequence of processes which
constitute the development of a cell of
a higher plant is given in the next
slide
 It is also applicable to tissues/organs.
40
Sequence of the developmental process in a plant
cell
41
HETEROPHYLLY
AND PLASTICITY
42
 Plants follow different pathways in response
to environment or phases of life to form
different kinds of structures. This ability is
called plasticity e.g. heterophylly in cotton,
coriander and larkspur
 In such plants, the leaves of the juvenile plant are
different in shape from those in mature plants
 On the other hand, difference in shapes of leaves
produced in air and those produced in water in
buttercup also represent the heterophyllous
development due to environment (Figure on the
next slide)
 This phenomenon of heterophylly is an
example of plasticity
…continue
d
Heterophylly in (a) larkspur and (b) buttercup 43
…continue
d
44
 Thus, growth, differentiation and development
are very closely related events in the life of a
plant
 Broadly, development is considered as the
sum of growth and differentiation
 Development in plants (i.e., both growth and
differentiation) is under the control of intrinsic
and extrinsic factors
 The former includes both intracellular
(genetic) or intercellular factors (chemicals
such as plant growth regulators) while the
latter includes light, temperature, water,
oxygen, nutrition, etc
PLANT GROWTH
REGULATORS(PG
R)
45
 The plant growth regulators (PGRs)
are small, simple molecules of diverse
chemical composition
 They could be indole compounds
(indole- 3-acetic acid, IAA); adenine
derivatives (N6-furfurylamino purine,
kinetin), derivatives of carotenoids
(abscisic acid, ABA); terpenes
(gibberellic acid, GA3) or gases
(ethylene, C2H4)
…continue
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46
 Plant growth regulators are variously
described as plant growth substances,
plant hormones or phytohormones in
literature
 The PGRs can be broadly divided into
two groups based on their functions in a
living plant body
 One group of PGRs are involved in
growth promoting activities, such as cell
division, cell
enlargement, pattern
formation,
tropic
see
d
growth, flowering, fruiting
and formation
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47
 These are also called plant growth promoters,
e.g., auxins, gibberellins and cytokinins
 The PGRs of the other group play an
important role in plant responses to wounds
and stresses of biotic and abiotic origin.
 They are also involved in various growth
inhibiting activities such as dormancy and
abscission
 The PGR abscisic acid belongs to this group
 The gaseous PGR, ethylene, could fit either
of the groups, but it is largely an inhibitor of
growth activities
The Discovery of Plant Growth
Regulators
48
 Interestingly, the discovery of each of
the five major groups of PGRs have
been accidental
 All this started with the observation of
Charles Darwin and his son
Francis Darwin when
they
observed that
the
coleoptiles of canary grass
responded to unilateral illumination by
growing towards the light source
(phototropism)
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49
 After a series of experiments, it was
concluded that the tip of coleoptile was
the site of transmittable influence that
caused the bending of the entire
coleoptile
 Auxin was isolated by F.W. Went from
tips of coleoptiles of oat seedlings
Experiment used to demonstrate that tip of the
coleoptile is the source of auxin. Arrows
indicate direction of light
50
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51
 No curvature when coleoptile tip was
removed
 Placing an opaque cap on the
coleoptile tip prevented curvature
 Tip curves when covered with a
transparent cap
 Experiment was performed on
coleoptiles of germinating oat-Avena
and canary grass- Phalaris
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52
 The ‘bakane’ (foolish seedling) a
disease of rice seedlings, was caused
by a fungal pathogen Gibberella
fujikuroi
 E. Kurosawa reported the appearance
of symptoms of the disease in
uninfected rice seedlings when they
were treated with sterile filtrates of the
fungus. The active substances were
later identified as gibberellic acid
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53
 F. Skoog and his co-workers observed
that from the internodal segments of
tobacco stems the callus (a mass of
undifferentiated cells) proliferated only if,
in addition to auxins the nutrients
medium was supplemented with one of
the following: extracts of vascular
tissues, yeast extract, coconut milk or
DNA.
 Skoog and Miller, later identified and
crystallised the cytokinesis promoting
active substance that they termed
kinetin
…continue
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54
 During mid-
1960s,
thre
e
independe
nt
researches reported the purification and
chemical characterisation of three
different kinds of inhibitors: inhibitor-B,
abscission II and dormin. Later all the
three were proved to be chemically
identical. It was named abscisic acid
(ABA)
 Cousins confirmed the release of a
volatile substance from ripened oranges
that hastened the ripening of stored
unripened bananas. Later this volatile
substance was identified as ethylene, a
gaseous PGR
Auxin
s
55
 Auxins (from Greek ‘auxein’ : to grow)
was first isolated from human urine. The
term ‘auxin’ is applied to the Indole-3-
acetic acid (IAA), and to other natural
and synthetic compounds having certain
growth regulating properties
 They are generally produced by the
growing apices of the stems and roots,
from where they migrate to the regions
of their action
 Auxins like IAA and Indole butyric acid
(IBA) have been isolated from plants
56
…continue
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57
 In most higher plants, the growing
apical bud inhibits the growth of the
lateral (axillary) buds, a phenomenon
called apical dominance
 Removal of shoot tips (decapitation)
usually results in the growth of lateral
buds
 It is widely applied in tea plantations,
hedge-making
…continue
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58
 NAA (naphthalene acetic acid) and 2, 4-D
(2, 4-dichlorophenoxyacetic) are
synthetic auxins
 All these auxins have been used
extensively in agricultural and
horticultural practices
 They help to initiate rooting in stem
cuttings, an application widely used for
plant propagation
 Auxins promote flowering e.g. in
pineapples
 They help to prevent fruit and leaf drop at
early stages but promote the abscission
5
Apical dominance in plants : (a) A plant with
apical bud intact (b) A plant with apical bud
removed Note the growth of lateral buds into
branches after decapitation.
…continue
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60
 Auxins also induce parthenocarpy,
e.g., in tomatoes
 They are widely used as herbicides
 2, 4-D, widely used to kill
dicotyledonous weeds, does not
affect mature monocotyledonous
plants
 It is used to prepare weed-free lawns
by gardeners
 Auxin also controls xylem
differentiation and helps in cell
GIBBERELLI
NS
61
 Gibberellins are another kind of
promotory PGR
 There are more than 100 gibberellins
reported from widely different organisms
such as fungi and higher plants
 They are denoted as GA1, GA2, GA3
and so on
 However, Gibberellic acid (GA3) was one
of the first gibberellins to be discovered
and remains the most intensively studied
form
GA 3
62
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63
 All GAs are acidic
 They produce a wide range of
physiological responses in the plants
 Their ability to cause an increase in length of
axis is used to increase the length of grapes
stalks
 Gibberellins, cause fruits like apple
to elongate and improve its shape
 They also delay senescence
 Thus, the fruits can be left on the tree longer
so as to extend the market period.
 GA3 is used to speed up the malting process
in brewing industry
…continue
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65
 Sugarcane stores carbohydrate as sugar in
their stems
 Spraying sugarcane crop with gibberellins
increases the length of the stem, thus
increasing the yield by as much as 20 tonnes
per acre
 Spraying juvenile conifers with GAs hastens
the maturity period, thus leading to early
seed production
 Gibberellins also promotes bolting (internode
elongation just prior to flowering) in beet,
cabbages and many plants with rosette habit
Cytokinin
s
66
 Cytokinins have specific effects on
cytokinesis, and were discovered as
kinetin (a modified form of adenine, a
purine) from the autoclaved herring
sperm DNA
 Kinetin does not occur naturally in plants.
Search for natural substances with
cytokinin- like activities led to the
isolation of zeatin from corn-kernels and
coconut milk
 Since the discovery of zeatin, several
naturally occurring cytokinins, and some
synthetic compounds with cell division
promoting activity, have been identified
67
…continue
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68
 High cytokinin to auxin ratio
causes differentiation of shoots
 A low ratio of cytokinin to
auxin causes root formation
 Intermediate cytokinin to auxin
ratio causes formation of roots
as well as shoots
 Intermediate cytokinin to low
auxin causes growth of large
amount of callus
…continue
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69
 Natural cytokinins are synthesised in
regions where rapid cell division occurs,
for example, root apices, developing
shoot buds, young fruits etc.
 It helps to produce new leaves,
chloroplasts
adventitious shoot
formation.
 Cytokinins help
overcome
in leaves, lateral shoot
growth
the
and
apic
al
dominance.
 They promote nutrient mobilisation
which helps in the delay of leaf
senescence.
…continue
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70
 Cytokinins break the dormancy of many seeds
and promote their germination
 Cytokinins stimulate the growth of lateral buds and
thus suppress apical dominance
 Cytokinins delay ageing of plant organs by
controlling protein synthesis and mobilisation of
resources
 Cytokinins promote femaleness during sex
expression in the flowering of plants
 Cytokinins induce parthenocarpy in many plants
…continue
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71
know
n
to
stimulat
e
sever
al
 Cytokinins
are enzymes
especially
thos
e
concerne
d
with
photosynthesis
 Cytokinins are used to
senescence in crop
plants
 Cytokinins are
applied
prevent
prematur
e
to the
marketed
vegetables, cut shoots and flowers to keep
them fresh for several days.
 Cytokinins are used in tissue culture
because of their role in cell division and
morphogenesis
Ethylen
e
72
 Ethylene is
a
simpl
e
gaseous PGR. It
is
synthesised in
large
amounts by
tissues
undergoing senescence and ripening fruits
 Influences of ethylene on plants include
horizontal growth of seedlings, swelling of the
axis and apical hook formation in dicot
seedlings
 Ethylene promotes senescence and
abscission of plant organs especially of
leaves and flowers
 Ethylene is highly effective in fruit ripening
 It enhances the respiration rate during
ripening of the fruits. This rise in rate of
respiration is called respiratory climactic
ETHYLENE AND ETHEPHON
73
…continue
d
74
 Ethylene breaks seed and bud
dormancy, initiates germination in
peanut seeds, sprouting of potato
tubers. Ethylene promotes rapid
internode/petiole elongation in deep
water rice plants. It helps leaves/
upper parts of the shoot to remain
above water
 Ethylene also promotes root growth
and root hair formation, thus helping
the plants to increase their absorption
surface
…continue
d
75
 Ethylene is used to initiate flowering
and for synchronising fruit-set in
pineapples. It also induces flowering
in mango
 Since ethylene regulates so many
physiological processes, it is one of
the most widely used PGR in
agriculture. The most widely used
compound as source of ethylene is
ethephon
…continue
d
76
 Ethephon in an aqueous solution is
readily absorbed and transported
within the plant and releases
ethylene slowly
 Ethephon hastens fruit ripening in
tomatoes and apples and accelerates
abscission in flowers and fruits
(thinning of cotton, cherry,walnut)
 It promotes female flowers in
cucumbers thereby increasing the
Abscisic
acid
77
 Abscisic acid (ABA) was discovered
for its role in regulating abscission
and dormancy. But like other PGRs, it
also has other wide ranging effects on
plant growth and development
 It acts as a general plant growth
inhibitor and an inhibitor of plant
metabolism.ABA inhibits seed
germination
ABSCISIC ACID
78
…continue
d
79
 ABA stimulates the closure of stomata in
the epidermis and increases the
tolerance of plants to various kinds of
stresses. Therefore, it is also called the
stress hormone.
 ABA plays an important role in seed
development, maturation and dormancy.
 By inducing dormancy, ABA helps seeds
to withstand desiccation and other
factors unfavourable for growth.
 In most situations, ABA acts as an
antagonist to GAs.
INTERACTION OF GROWTH
HORMONES
80
 For any and every phase of growth,
differentiation and development of plants,
one or the other PGR has some role to
play. Such roles could be complimentary
or antagonistic. These could be
individualistic or synergistic
 Similarly, there are a number of events in
the life of a plant where more than one
PGR interact to affect that event, e.g.,
dormancy in seeds/ buds, abscission,
senescence, apical dominance, etc
…continue
d
81
 Remember, the role of PGR is of only
one kind of intrinsic control
 Along with genomic control and extrinsic
factors, they play an important role in
plant growth and development. Many of
the extrinsic factors such as temperature
and light, control plant growth and
development via PGR. Some of such
events could be: vernalisation, flowering,
dormancy, seed germination, plant
movements,etc
82

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PLANT GROWTH AND DEVELOPMENT 2022

  • 3. …continue d 3  The first step to the process of plant growth is seed germination  It is one of the most fundamental and conspicuous characteristics of living organisms  It is the net result of various processes that together cause an irreversible permanent increase in the size of of an organ or its parts
  • 4. …continue d 4  Growth is accompanied by anabolism and catabolism that occur in the body at the expense of energy  It is intrinsic or internal  It is generally indeterminate  This is due to meristems  Cells derived from meristems grow in size, differentiate and form the bulk of the plant body
  • 5. …continue d 5  Growth by meristematic activity is called open form of growth  Apical shoot, root meristems and intercalary meristems cause primary growth  In dicotyledons and gymnosperms the lateral meristems, vascular cambium and cork cambium appear later in life but are indeterminate causing secondary growth
  • 6. MERISTEMS INDETERMINATE MERISTEMS DETERMINATE MERISTEMS  Root apical meristem, shoot apical meristem, and lateral meristem are active throughout the life of the plant and are thus indeterminate meristems  The intercalary meristem 6 present in leaf and flower primordia ha s a shor t period of activity and is consumed in the formation of organs and is thus of the determinate type
  • 7. 7
  • 8. UNLIMITED GROWTH IN A WOODY PERENNIAL 8
  • 10. FORMATIVE PHASE 10  Phase of cell formation or division  Occurs at the root apex,shoot apex and other regions with meristematic tissues  New cells are produced by Mitosis  Rate of cellular respiration is very high  Some daughter cells retain the capacity to divide while the others enter the next growth phase  Due to the increase in the cell number the areas where divisions occur show some growth
  • 11. PHASE OF ELONGATION OR ENLARGEMENT 11  Cells formed in the formative phase enlarge  Cell walls grow by deposition of new cell wall materials  A central vacuole appears  At this stage some protoplasm is added  In isodiametric cells there is significant growth axially so that roots and stems elongate, though they enlarge in all directions  This phase corresponds to the exponential phase  Rate of respiration is high but lesser than in the previous phase
  • 12. PHASE OF DIFFERENTIATION 12  Cell wall grows in thickness and gradually undergoes structural and physiological changes as per its location  Formation of root hair, elongation of xylem and phloem fibres, guard cells of stomatal epidermi s, apparatus, cutinisation of chloroplasts in mesophyll, suberisation of cork cell etc are examples of structural differentiation
  • 13. …continue d  Root hair take up the function of absorption, mesophyll cells photosynthesise, xylem and phloem bring about ascent of sap and translocation of food respectively  Mature cells do not grow further after differentiation  NOTE: THE TIME PERIOD FROM THE MERISTEMATIC PHASE TILL THE MATURATION PHASE IS THE GRAND PERIOD OF GROWTH 13
  • 14. MEASUREMENT OF GROWTH  Growth in plants is measured in terms of: Increase in the number of cells produced Increase in the girth and length of shoot and root Increase in the dry weight of organ and increase in the area of leaf or volume of a fruit 14
  • 15. MEASUREMENT OF GROWTH IN TERMS OF LENGTH AUXANOMETER ARCAUXANOMETER PFEFFER’SAUXANOMETER HORIZONTAL MICROSCOPE FIXED AT A CLOSE DISTANCE TO THE GROWING APEX AND AFTER A LAPSE OF TIME MOVED UP DISTANCE OF UPWARD MOVEMENT= GROWTH DIRECT METHOD 15 WITH THE HELP OFA SCALE TO FIND CHANGE IN LENGTH AFTER PARTICULAR TIME INTERVALS
  • 17. ARC AUXANOMETER 17  Consists of a curved graduated scale, a pulley with a groove and a pointer which moves on a scale  One end of a thread is tied to the stem tip of a potted plant and then hung on the pulley of the auxanometer  The other end is tied of the thread is tied to a small weight to keep the thread stretched
  • 18. 18
  • 19. …continue d 19  The pulley is fixed with a long aluminium pointer which slides over a graduated arc  As the stem grows in length the weight pulls the thread down  The pointer moves on the graduated arc indicating growth
  • 20. Figure 1 An auxanometer is a device for making automated measurements of growth. A figure of a late 19th century auxanometer taken from Wilhelm Pfeffer's classic textbook is shown [<ce:cross-ref refid="bib0005"> 1</ce:cross-ref> ]. 20 Edgar P Spalding , Nathan D Miller Current Opinion in Plant Biology Volume 16, Issue 1 2013 100 - 104 http://dx.doi.org/10.1016/j.pbi.2013.01.001 PFEFFER’SAUXANOMETER
  • 21. GROWTH RATES  Growth in unit time is growth rate GROWTH RATES ARITHMETI C Lt = L0 + rt GEOMETRI C W1 =W0ert 21
  • 22. Arithmetic Growth 22  Growth is constant from the beginning  Growth progresses arithmetically e.g. 2to4to6to 8to10  Found in root and shoot apices  Single meristematic cell divides into two of which one continues to divide while the other differentiates and matures  Lt = L0 + rt Lt = Length after time t
  • 23. …continue d 23 L0 = length at the beginning  r = growth rate or elongation per unit time
  • 24. LINEAR CURVE OF ARITHMETIC GROWTH 24
  • 25. GEOMETRIC GROWTH 25  All daughter cells of the mother cell divide  Common in unicellular organisms and microorganisms  The initial growth is slow (lag phase), and it increases rapidly thereafter at an exponential rate (exponential phase).  With limited nutrient supply, the growth slows down after sometime (stationary phase) or it may stop
  • 26. …continue d 26  A typical S-shaped or sigmoid growth curve is obtained in organisms living in a natural environment  It is not uniform in plants showing seasonal activity showing small steps indicating stoppage and resumption of growth every year  W1 =W0ert  W0 = initial size at the beginning
  • 27. …continue d 27  W1 = final size(weight, height , number)  r = growth rate  t= time for which the growth continues  e = base of natural logarithms  The magnitude of growth rate is called efficiency index by Blackman(1919) as organisms with higher growth rates will outperform others
  • 29. C = stages during embryonic development showing both the types of growth rates 29
  • 30. Absolute growth rate 30  It is the measurement and comparison in total growth of two organs or organisms per unit time  A bell shaped curve is obtained during the grand period of growth  Initial rise is lag and log phases  The top Gmax  Declining curve = declining growth
  • 31. BELL SHAPED ABSOLUTE GROWTH RATECURVE 31
  • 32. Relative Growth Rate 32  Growth rate per unit time shown as percentage of initial size  RGR = Growth in a given time period *100 Initial size  In the next slide AGR is same in both the leaves while RGR of leaf A is faster due to its smaller size
  • 33. Diagrammatic comparison of absolute and relative growth rates. Both leaves A and B have increased their area by 5 sq.cm in a given time to produce A’,B’leaves. 33
  • 34. FACTORS AFFECTING PLANT GROWTH  SUPPLY OF NUTRIENTS: Nutrients (macro and micro essential elements) are required by plants for the synthesis of protoplasm and act as source of energy  WATER: Turgidity of cells helps in extension growth. Thus, plant growth and further development is intimately linked to the water status of the plant. Water also provides the medium for enzymatic activities needed for growth 34
  • 35. …continue d 35 in releasin g for growth  OXYGEN: Oxygen helps metabolic energy essential activities  SUITABLE TEMPERATURE: 28°C to 30°C is optimum for plant growth. Low temperature inactivates enzymes and a temperature above 45°C hinders growth protoplasm an d by denaturing enzymes, causin g coagulati ng excessi ve transpiratio n
  • 36. …continue d 36  C:N RATIO: Regulates specific growth patterns  For example, presence of more N and less Carbohydrates synthesises more protoplasm and less mechanical tissues with vigorous mechanical growth  Leaves of such plants are succulent, large and dark green  If C:N ratio is larger, then more cell wall materials and mechanical tissues are synthesised
  • 37. …continue d 37 intensity, duration and direct effect on plant  LIGHT: light quality have a growth  Young plants get etiolated in absence of light  Environmental signals such as light and gravity also affect certain phases/stages of growth.
  • 38. DIFFERENTIATION, DEDIFFERENTIATION AND REDIFFERENTIATION 38  The cells derived from root apical and shoot- apical meristems and cambium differentiate and mature to perform specific functions  This act leading to maturation is termed as differentiation  During differentiation, cells undergo few to major structural changes both in their cell walls and protoplasm. For example, to form a tracheary element, the cells would lose their protoplasm. They also develops a very strong, elastic, lignocellulosic secondary cell walls, to carry water to long distances even under extreme tension
  • 39. …continue d 39  Plants show another interesting phenomenon.  The living differentiated cells, that by now have lost the capacity to divide can regain the capacity of division under certain conditions. This phenomenon is termed as dedifferentiation  For example, formation of meristems – interfascicular cambium and cork cambium from fully differentiated parenchyma cells  While doing so, such meristems/tissues are able to divide and produce cells that once again lose the capacity to divide but mature to perform specific functions, i.e., get redifferentiated
  • 40. DEVELOPME NT  Development is a term that includes all changes that an organism goes through during its life cycle from germination of the seed to senescence  Diagrammatic representation of the sequence of processes which constitute the development of a cell of a higher plant is given in the next slide  It is also applicable to tissues/organs. 40
  • 41. Sequence of the developmental process in a plant cell 41
  • 42. HETEROPHYLLY AND PLASTICITY 42  Plants follow different pathways in response to environment or phases of life to form different kinds of structures. This ability is called plasticity e.g. heterophylly in cotton, coriander and larkspur  In such plants, the leaves of the juvenile plant are different in shape from those in mature plants  On the other hand, difference in shapes of leaves produced in air and those produced in water in buttercup also represent the heterophyllous development due to environment (Figure on the next slide)  This phenomenon of heterophylly is an example of plasticity
  • 43. …continue d Heterophylly in (a) larkspur and (b) buttercup 43
  • 44. …continue d 44  Thus, growth, differentiation and development are very closely related events in the life of a plant  Broadly, development is considered as the sum of growth and differentiation  Development in plants (i.e., both growth and differentiation) is under the control of intrinsic and extrinsic factors  The former includes both intracellular (genetic) or intercellular factors (chemicals such as plant growth regulators) while the latter includes light, temperature, water, oxygen, nutrition, etc
  • 45. PLANT GROWTH REGULATORS(PG R) 45  The plant growth regulators (PGRs) are small, simple molecules of diverse chemical composition  They could be indole compounds (indole- 3-acetic acid, IAA); adenine derivatives (N6-furfurylamino purine, kinetin), derivatives of carotenoids (abscisic acid, ABA); terpenes (gibberellic acid, GA3) or gases (ethylene, C2H4)
  • 46. …continue d 46  Plant growth regulators are variously described as plant growth substances, plant hormones or phytohormones in literature  The PGRs can be broadly divided into two groups based on their functions in a living plant body  One group of PGRs are involved in growth promoting activities, such as cell division, cell enlargement, pattern formation, tropic see d growth, flowering, fruiting and formation
  • 47. …continue d 47  These are also called plant growth promoters, e.g., auxins, gibberellins and cytokinins  The PGRs of the other group play an important role in plant responses to wounds and stresses of biotic and abiotic origin.  They are also involved in various growth inhibiting activities such as dormancy and abscission  The PGR abscisic acid belongs to this group  The gaseous PGR, ethylene, could fit either of the groups, but it is largely an inhibitor of growth activities
  • 48. The Discovery of Plant Growth Regulators 48  Interestingly, the discovery of each of the five major groups of PGRs have been accidental  All this started with the observation of Charles Darwin and his son Francis Darwin when they observed that the coleoptiles of canary grass responded to unilateral illumination by growing towards the light source (phototropism)
  • 49. …continue d 49  After a series of experiments, it was concluded that the tip of coleoptile was the site of transmittable influence that caused the bending of the entire coleoptile  Auxin was isolated by F.W. Went from tips of coleoptiles of oat seedlings
  • 50. Experiment used to demonstrate that tip of the coleoptile is the source of auxin. Arrows indicate direction of light 50
  • 51. …continue d 51  No curvature when coleoptile tip was removed  Placing an opaque cap on the coleoptile tip prevented curvature  Tip curves when covered with a transparent cap  Experiment was performed on coleoptiles of germinating oat-Avena and canary grass- Phalaris
  • 52. …continue d 52  The ‘bakane’ (foolish seedling) a disease of rice seedlings, was caused by a fungal pathogen Gibberella fujikuroi  E. Kurosawa reported the appearance of symptoms of the disease in uninfected rice seedlings when they were treated with sterile filtrates of the fungus. The active substances were later identified as gibberellic acid
  • 53. …continue d 53  F. Skoog and his co-workers observed that from the internodal segments of tobacco stems the callus (a mass of undifferentiated cells) proliferated only if, in addition to auxins the nutrients medium was supplemented with one of the following: extracts of vascular tissues, yeast extract, coconut milk or DNA.  Skoog and Miller, later identified and crystallised the cytokinesis promoting active substance that they termed kinetin
  • 54. …continue d 54  During mid- 1960s, thre e independe nt researches reported the purification and chemical characterisation of three different kinds of inhibitors: inhibitor-B, abscission II and dormin. Later all the three were proved to be chemically identical. It was named abscisic acid (ABA)  Cousins confirmed the release of a volatile substance from ripened oranges that hastened the ripening of stored unripened bananas. Later this volatile substance was identified as ethylene, a gaseous PGR
  • 55. Auxin s 55  Auxins (from Greek ‘auxein’ : to grow) was first isolated from human urine. The term ‘auxin’ is applied to the Indole-3- acetic acid (IAA), and to other natural and synthetic compounds having certain growth regulating properties  They are generally produced by the growing apices of the stems and roots, from where they migrate to the regions of their action  Auxins like IAA and Indole butyric acid (IBA) have been isolated from plants
  • 56. 56
  • 57. …continue d 57  In most higher plants, the growing apical bud inhibits the growth of the lateral (axillary) buds, a phenomenon called apical dominance  Removal of shoot tips (decapitation) usually results in the growth of lateral buds  It is widely applied in tea plantations, hedge-making
  • 58. …continue d 58  NAA (naphthalene acetic acid) and 2, 4-D (2, 4-dichlorophenoxyacetic) are synthetic auxins  All these auxins have been used extensively in agricultural and horticultural practices  They help to initiate rooting in stem cuttings, an application widely used for plant propagation  Auxins promote flowering e.g. in pineapples  They help to prevent fruit and leaf drop at early stages but promote the abscission
  • 59. 5 Apical dominance in plants : (a) A plant with apical bud intact (b) A plant with apical bud removed Note the growth of lateral buds into branches after decapitation.
  • 60. …continue d 60  Auxins also induce parthenocarpy, e.g., in tomatoes  They are widely used as herbicides  2, 4-D, widely used to kill dicotyledonous weeds, does not affect mature monocotyledonous plants  It is used to prepare weed-free lawns by gardeners  Auxin also controls xylem differentiation and helps in cell
  • 61. GIBBERELLI NS 61  Gibberellins are another kind of promotory PGR  There are more than 100 gibberellins reported from widely different organisms such as fungi and higher plants  They are denoted as GA1, GA2, GA3 and so on  However, Gibberellic acid (GA3) was one of the first gibberellins to be discovered and remains the most intensively studied form
  • 63. …continue d 63  All GAs are acidic  They produce a wide range of physiological responses in the plants  Their ability to cause an increase in length of axis is used to increase the length of grapes stalks  Gibberellins, cause fruits like apple to elongate and improve its shape  They also delay senescence  Thus, the fruits can be left on the tree longer so as to extend the market period.  GA3 is used to speed up the malting process in brewing industry
  • 64.
  • 65. …continue d 65  Sugarcane stores carbohydrate as sugar in their stems  Spraying sugarcane crop with gibberellins increases the length of the stem, thus increasing the yield by as much as 20 tonnes per acre  Spraying juvenile conifers with GAs hastens the maturity period, thus leading to early seed production  Gibberellins also promotes bolting (internode elongation just prior to flowering) in beet, cabbages and many plants with rosette habit
  • 66. Cytokinin s 66  Cytokinins have specific effects on cytokinesis, and were discovered as kinetin (a modified form of adenine, a purine) from the autoclaved herring sperm DNA  Kinetin does not occur naturally in plants. Search for natural substances with cytokinin- like activities led to the isolation of zeatin from corn-kernels and coconut milk  Since the discovery of zeatin, several naturally occurring cytokinins, and some synthetic compounds with cell division promoting activity, have been identified
  • 67. 67
  • 68. …continue d 68  High cytokinin to auxin ratio causes differentiation of shoots  A low ratio of cytokinin to auxin causes root formation  Intermediate cytokinin to auxin ratio causes formation of roots as well as shoots  Intermediate cytokinin to low auxin causes growth of large amount of callus
  • 69. …continue d 69  Natural cytokinins are synthesised in regions where rapid cell division occurs, for example, root apices, developing shoot buds, young fruits etc.  It helps to produce new leaves, chloroplasts adventitious shoot formation.  Cytokinins help overcome in leaves, lateral shoot growth the and apic al dominance.  They promote nutrient mobilisation which helps in the delay of leaf senescence.
  • 70. …continue d 70  Cytokinins break the dormancy of many seeds and promote their germination  Cytokinins stimulate the growth of lateral buds and thus suppress apical dominance  Cytokinins delay ageing of plant organs by controlling protein synthesis and mobilisation of resources  Cytokinins promote femaleness during sex expression in the flowering of plants  Cytokinins induce parthenocarpy in many plants
  • 71. …continue d 71 know n to stimulat e sever al  Cytokinins are enzymes especially thos e concerne d with photosynthesis  Cytokinins are used to senescence in crop plants  Cytokinins are applied prevent prematur e to the marketed vegetables, cut shoots and flowers to keep them fresh for several days.  Cytokinins are used in tissue culture because of their role in cell division and morphogenesis
  • 72. Ethylen e 72  Ethylene is a simpl e gaseous PGR. It is synthesised in large amounts by tissues undergoing senescence and ripening fruits  Influences of ethylene on plants include horizontal growth of seedlings, swelling of the axis and apical hook formation in dicot seedlings  Ethylene promotes senescence and abscission of plant organs especially of leaves and flowers  Ethylene is highly effective in fruit ripening  It enhances the respiration rate during ripening of the fruits. This rise in rate of respiration is called respiratory climactic
  • 74. …continue d 74  Ethylene breaks seed and bud dormancy, initiates germination in peanut seeds, sprouting of potato tubers. Ethylene promotes rapid internode/petiole elongation in deep water rice plants. It helps leaves/ upper parts of the shoot to remain above water  Ethylene also promotes root growth and root hair formation, thus helping the plants to increase their absorption surface
  • 75. …continue d 75  Ethylene is used to initiate flowering and for synchronising fruit-set in pineapples. It also induces flowering in mango  Since ethylene regulates so many physiological processes, it is one of the most widely used PGR in agriculture. The most widely used compound as source of ethylene is ethephon
  • 76. …continue d 76  Ethephon in an aqueous solution is readily absorbed and transported within the plant and releases ethylene slowly  Ethephon hastens fruit ripening in tomatoes and apples and accelerates abscission in flowers and fruits (thinning of cotton, cherry,walnut)  It promotes female flowers in cucumbers thereby increasing the
  • 77. Abscisic acid 77  Abscisic acid (ABA) was discovered for its role in regulating abscission and dormancy. But like other PGRs, it also has other wide ranging effects on plant growth and development  It acts as a general plant growth inhibitor and an inhibitor of plant metabolism.ABA inhibits seed germination
  • 79. …continue d 79  ABA stimulates the closure of stomata in the epidermis and increases the tolerance of plants to various kinds of stresses. Therefore, it is also called the stress hormone.  ABA plays an important role in seed development, maturation and dormancy.  By inducing dormancy, ABA helps seeds to withstand desiccation and other factors unfavourable for growth.  In most situations, ABA acts as an antagonist to GAs.
  • 80. INTERACTION OF GROWTH HORMONES 80  For any and every phase of growth, differentiation and development of plants, one or the other PGR has some role to play. Such roles could be complimentary or antagonistic. These could be individualistic or synergistic  Similarly, there are a number of events in the life of a plant where more than one PGR interact to affect that event, e.g., dormancy in seeds/ buds, abscission, senescence, apical dominance, etc
  • 81. …continue d 81  Remember, the role of PGR is of only one kind of intrinsic control  Along with genomic control and extrinsic factors, they play an important role in plant growth and development. Many of the extrinsic factors such as temperature and light, control plant growth and development via PGR. Some of such events could be: vernalisation, flowering, dormancy, seed germination, plant movements,etc
  • 82. 82