Based on shape and appearance: 3 types Fibroblast-like Epithelial-like Lymphoblast-like

1. Morphology and
types of Cells in
Culture
LECTURE OF SUBJECT:
Dr. sharafaldin Al-musawi
College of Biotecholgy
LECTURE: 3
SUBJECT: Animal Tissue culture
LEVEL: 4

2. • Fibroblast-like
• Epithelial-like
• Lymphoblast-like
Based on shape and appearance: 3 types

3.  Fibroblast-like cells are
bipolar or multipolar, have
elongated shapes, and grow
attached to a substrate
Morphology of Cells in Culture

4. •Epithelial-like cells are
polygonal in shape with
more regular dimensions,
and grow attached to a
substrate in discrete
patches.
Morphology of Cells in Culture

5. •Lymphoblast-like cells are
spherical in shape and
usually grown in suspension
without attaching to a
surface.
Morphology of Cells in Culture

6.  Anchorage-dependent
 Anchorage-independent
Morphology of Cells in Culture
 Depending on the adherence property : 2 types

7.  Anchorage-dependent (adherent or
monolayer culture):
Must be cultured while attached to a solid or semi-
solid substrate
Example: MCF7, PC-3
 Anchorage-Independent (suspension
culture):
Can be grown floating in the culture medium
Example: K562
Morphology of Cells in Culture