12. USING ABSORBING PAPER STRIPS
(Intrasulcular Method)
Brill Technique
((Loe & Holm-Pedersen)
13.
14. Preweighed twisted thread
• Used by Weinstein
• Threads are placed in gingival crevice around the tooth & amount of
fluid collected estimated by weighing the sample thread
15. Capillary tubes/Micropipettes
Collected using capillary action
Since diameter is known volume of fluid collected is determined by measuring
the distance which GCF has migrated.
Ideal technique for undiluted sample of native GCF
Disadvantage
Difficulty in removal of complete sample from tubing
16.
17. Crevicular washings
Other with use of peristaltic pump
A modification of the this
method is use of 2 injection
needles fitted one within the
other so that during sampling
the inside needle is at the
bottom of pocket and outside
needle is at gingival margin
The collection needle is
drained into a sample tube via
continuous suction.
18.
19.
20.
21. Electronic method
• This measure the fluid collected on a blotter (periopaper) with help of an
electronic transducer( periotron)
3 models of periotron
600
6000
8000
Limitation –inability to measure volumes of GCF greater than 1.0μL
22. Problems with GCF collections & data
interpretation are
• Contamination
• Sample time
• Volume determination –evaporation
• Recovery from strips-binding of GCF protein to filterpaper.Complete
recovery difficult
23. Cellular & humoral immunity in GCF
• Cellular immunity
• Includes appearance of cytokines in GCF
• Eg IL-1α,IL -1β
24. • Role of antibodies is difficult to ascertain ….
• Reduction in antibody level is detrimental to periodontal health
