Professor Roger Butlin from The University of Sheffield (UK) presents the first seminar in the ACEBB Science Seminar Series entitled "Winkles and the origin of species".

1. ACEBB Seminar Series Winkles and the origin of species Professor Roger Butlin

5. Winkles and the origin of species Roger Butlin The University of Sheffield

7. Ernst Haeckel 1866 Ernst Haeckel 1906

8. Barton et al. 2007

10. Species 1 Space Species 2 Time

11. Species 1 Species 2

12. Species 1 Species 2 Chorthippusparallelus

13. Species 1 Bombinavariegata Species 2 Bombinabombina

14. Drosophila simulans Species 1 Species 2 Jones lab

15. Species 1 Wu 2001 Species 2

16. Species 1 What initiates the evolution of reproductive isolation? What completes the evolution of reproductive isolation? What drives the increase in reproductive isolation? Species 2

17. Butlin et al. 2008

18. Littorinasaxatilis ‘H’ ‘M’ Thornwick Bay

19. Independentgradients in Spain and Sweden E (exposed) S (sheltered) Kerstin Johannesson, Göteborg University SU RB Emilio Rolan-AlvarezUniversity of Vigo LOWER MID UPPER Roger Butlin

20. Scale dependent!

21. Sympatric and parallel or allopatric and interdependent? Quesada et al. 2007Evolution

22. Old Peak H Thornwick Bay H Filey H 87 99 Robin Hood’s Bay 2 90 Thornwick Bay M 76 Robin Hood’s Bay 1 88 Old Peak M Filey M 0.01 290 AFLP loci

23. Wilding et al. 2001

24. Old Peak H Thornwick Bay M Filey H Thornwick Bay H Filey H Filey M 87 Old Peak H 73 99 99 67 Thornwick Bay H 99 Robin Hood’s Bay 2 81 Robin Hood’s Bay 2 90 Thornwick Bay M 76 Robin Hood’s Bay 1 88 Robin Hood’s Bay 1 Old Peak M Old Peak M 0.01 Filey M 0.01 All loci 15 ‘selected’ loci removed

25. Sympatric and parallel or allopatric and interdependent? All SS have haplotype 1regardless of locality! Petri Kempainnen thesis

26. BAC library Not differentiated, control (A30) Weakly differentiated (A37) Differentiated (E12) Most differentiated (E10) ‘H’ ‘M’ Littorinasaxatilis Wilding et al 2001 Grahame et al 2006

27. Predicted gene AFLP Region sequenced A B C D

28. 5.3 * 3.0 4.3 * 0 11.8 6.9 * 6.2 4.2 * 6.0 kb FST=0.00 FST=0.12 FST=0.32 FST=0.54

29. Similar to tetraspanin 3A of Drosophila Conserved protein of unknown function AFLP E10L0 E10L1 120kb 130kb 140kb

30. Pea aphid host races on clover and alfalfaVia and West (2008)

31. 454 – based genome scan - 15 individuals / sample - RNA extraction (whole body) H M Juan Galindo

32. Divergent SNPs

33. 2454 SNPs in 572 contigs 510 SNPs in 197 contigs Outlier (P<0.05 by simulation with ‘Winkle454’) Significant after SGoF correction (http://webs.uvigo.es/acraaj/SGoF.htm) Significant after Benjamini-Hochberg correction *

36. Transposable elements?

37. Some interesting proteins…Shell formation Lithostatine Mucin Dermatopontin Muscle physiology Myosin Titin Energetic metabolism Argininekinase NADH dehydrogenase

39. Thanks! John Grahame, Craig Wilding, Henry Wood and Juan Galindo – Littorina NERC and BBSRC – cash This sketch is most imperfect; but in so short a space I cannot make it better. Your imagination must fill up very wide blanks. Darwin, 1858

40. Next seminar A system for establishing TERN in Australia Associate Professor Jean-Marc Hero