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James M. Mitchell, MD
VIROLOGY
VIROLOGY
VIROLOGY
Viruses
 Definitions:
 Virus- acellular, infectious agent having one or more pieces of
nucleic acids, DNA or RNA but not both; wrapped in a protein coat,
+/- membranous envelope; obligate intracellular parasite
 Bacteriophage (phage)- a virus that infects bacteria
 Viral Replication
 Lytic- replication involves use of the host cell’s organelles and enzymes
to produce virions resulting in the death and lysis of the host cell; results
in cell death
 Lysogenic- viral DNA is inserted into the host genome; it is copied when
the cell replicates and passed to daughter cells; an external stressor can
induce the cell into the lytic phase
 Transduction- transfer of bacterial genes by a phage
 Prophage- insertion of phage DNA into bacterial chromosome
Viruses
VIRUSES
 Background History
 Discovered in 1892 by Russian botanist Dmitri Ivanosky
 Tobacco mosaic virus (TMV)
 Hershey and Chase- in 1952 demonstrated that DNA is
the genetic material of a bacteriophage (T2) which
infects the bacterium Escherichia coli (E. coli)
Viruses
Viruses
 Characteristics of Viruses
 Similarities
 Intracellular/extracellular- virion
 Protein Coat- capsid
 Membrane-like envelope- not all
Viruses
Viruses
 Characteristics of Viruses
 Differences
 Genetic Material
 Classification based on type of genetic material in viral
genome
 Double-stranded DNA (dsDNA)
 Single-stranded RNA (ssRNA)
 Single-stranded DNA (ssDNA)
 Double-stranded RNA (dsRNA)
Viruses
 Characteristics of Viruses
 Differences
 Hosts of Viruses
 Most viruses infect only particular host’s cells
 HIV- T helper cells
 Rabies- humans to bats
 Plant viruses- corn, sugarcane, tobacco, tomatoes, beans
 Fungal viruses
Viruses
 Characteristics of Viruses
 Differences
 Sizes- 10nm t0 300nm
 Poliovirus 30nm
 Smallpox virus 200nm x 300nm
Viruses
Viruses
 Characteristics of Viruses
 Capsid Morphology
 Composed of protein subunits- capsomeres
 Viral Shapes
 Helical, polyhedral, complex
 Viral Envelope
 Enveloped vs. Nonenveloped
 Acquired from the host cell during viral replication or release
 Plays a role in the recognition of host cells
Viruses
Viruses
Viruses
Viruses
Viruses
 Classification
1. Taxonomy
 Type of nucleic acid
 Presence of an envelope
 Shape
 Size
Viruses
 Retroviruses
 RNA viruses, +ssRNA
 Require reverse transcriptase to make a DNA intermediary
 mRNA is transcribed from the DNA by RNA polymerase
 DNA is incorporated into the host DNA (provirus)
 Examples-
 Rous Sarcoma Virus- infects chickens
 Simian Immunodeficiency Virus
 Feline Leukemia Virus
 HTLV- 1st human retrovirus discovered
 HIV
VIRUSES
Viruses
 Impact of Viruses
 Replicate quickly, mutate rapidly, remain hidden inside host
cells
 Antibiotics/Antivirals
 Threat of viral diseases
 Human
 Plant
 Cloning
 Oncogenes
 Burkitt’s Lymphoma- EBV
 Kaposi’s Sarcoma- HHV-8
 Cervical Cancer- HPV
 Hepatocarcinoma- HEP B
VIRUSES
Viruses
 References:
1. Bauman, R. W., & Timberlake, K. C. (2006).
Microbiology and chemistry: An integrated approach.
Boston, MA: Pearson Custom Publishing.
2. Campbell, N. A. (2009). Biology: Concepts &
connections. San Francisco: Pearson/Benjamin
Cummings.
3. Kasper, D. L., & Harrison, T. R. (2005). Harrison's
principles of internal medicine. New York: McGraw-
Hill, Medical Pub. Division.

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VIRUSES.pptx

  • 5. Viruses  Definitions:  Virus- acellular, infectious agent having one or more pieces of nucleic acids, DNA or RNA but not both; wrapped in a protein coat, +/- membranous envelope; obligate intracellular parasite  Bacteriophage (phage)- a virus that infects bacteria  Viral Replication  Lytic- replication involves use of the host cell’s organelles and enzymes to produce virions resulting in the death and lysis of the host cell; results in cell death  Lysogenic- viral DNA is inserted into the host genome; it is copied when the cell replicates and passed to daughter cells; an external stressor can induce the cell into the lytic phase  Transduction- transfer of bacterial genes by a phage  Prophage- insertion of phage DNA into bacterial chromosome
  • 7. VIRUSES  Background History  Discovered in 1892 by Russian botanist Dmitri Ivanosky  Tobacco mosaic virus (TMV)  Hershey and Chase- in 1952 demonstrated that DNA is the genetic material of a bacteriophage (T2) which infects the bacterium Escherichia coli (E. coli)
  • 9. Viruses  Characteristics of Viruses  Similarities  Intracellular/extracellular- virion  Protein Coat- capsid  Membrane-like envelope- not all
  • 11. Viruses  Characteristics of Viruses  Differences  Genetic Material  Classification based on type of genetic material in viral genome  Double-stranded DNA (dsDNA)  Single-stranded RNA (ssRNA)  Single-stranded DNA (ssDNA)  Double-stranded RNA (dsRNA)
  • 12. Viruses  Characteristics of Viruses  Differences  Hosts of Viruses  Most viruses infect only particular host’s cells  HIV- T helper cells  Rabies- humans to bats  Plant viruses- corn, sugarcane, tobacco, tomatoes, beans  Fungal viruses
  • 13. Viruses  Characteristics of Viruses  Differences  Sizes- 10nm t0 300nm  Poliovirus 30nm  Smallpox virus 200nm x 300nm
  • 15. Viruses  Characteristics of Viruses  Capsid Morphology  Composed of protein subunits- capsomeres  Viral Shapes  Helical, polyhedral, complex  Viral Envelope  Enveloped vs. Nonenveloped  Acquired from the host cell during viral replication or release  Plays a role in the recognition of host cells
  • 20. Viruses  Classification 1. Taxonomy  Type of nucleic acid  Presence of an envelope  Shape  Size
  • 21. Viruses  Retroviruses  RNA viruses, +ssRNA  Require reverse transcriptase to make a DNA intermediary  mRNA is transcribed from the DNA by RNA polymerase  DNA is incorporated into the host DNA (provirus)  Examples-  Rous Sarcoma Virus- infects chickens  Simian Immunodeficiency Virus  Feline Leukemia Virus  HTLV- 1st human retrovirus discovered  HIV
  • 23. Viruses  Impact of Viruses  Replicate quickly, mutate rapidly, remain hidden inside host cells  Antibiotics/Antivirals  Threat of viral diseases  Human  Plant  Cloning  Oncogenes  Burkitt’s Lymphoma- EBV  Kaposi’s Sarcoma- HHV-8  Cervical Cancer- HPV  Hepatocarcinoma- HEP B
  • 25. Viruses  References: 1. Bauman, R. W., & Timberlake, K. C. (2006). Microbiology and chemistry: An integrated approach. Boston, MA: Pearson Custom Publishing. 2. Campbell, N. A. (2009). Biology: Concepts & connections. San Francisco: Pearson/Benjamin Cummings. 3. Kasper, D. L., & Harrison, T. R. (2005). Harrison's principles of internal medicine. New York: McGraw- Hill, Medical Pub. Division.

Editor's Notes

  1. First described by Adolf Mayer 1886, 1898 Beijerinck
  2. Togaviridae, flaviviridae; helical Coronavirus