Vibrio cholerae is bacteria present in aquatic environment.It causes cholera,a diarrhoel disease.it is usually occur in developing countries.There are more than 200 serotypes are identified but two serotypes are more dangerous i.e.serotype O1 and serotype O139.The disease is endemic to certain regions where contaminated food and water are used for consumption purposes.The disease becomes epidemic due to the above two serotypes. Vibrio cholerae and zooplankton shows commensalism mainly with copepods,water fleas,daphnia,rotifers,ostracods etc. Zooplanktons are microscopic organism normally found in sea ,river,ponds. In Bakerganj and Mathbaria (Bangladesh) the population of zooplanktons were analysed and it was found that Mathbaria\'s river and ponds were actually reservoir for vibrio cholerae. In this areas the environment plays significant role in colonization of zooplankton by pathogenic strains. In these areas the water is used for drinking purposes without any treatment. Cholera is depend on doses.So increase in their numbers increases the chances of endemic or epidemic sometimes pandemics. In these areas flood comes regularly due to which sea water mixes with pond, river water that is also a major factor for the spread of disease. By using molecular genetics and genomic methods for the detection of zooplankton species and determination of selected association of V.cholerae may allow early warning warning of pathogenic strain of V.cholerae and treatment of water by just simple filtration can also helps in its prevention. Solution Vibrio cholerae is bacteria present in aquatic environment.It causes cholera,a diarrhoel disease.it is usually occur in developing countries.There are more than 200 serotypes are identified but two serotypes are more dangerous i.e.serotype O1 and serotype O139.The disease is endemic to certain regions where contaminated food and water are used for consumption purposes.The disease becomes epidemic due to the above two serotypes. Vibrio cholerae and zooplankton shows commensalism mainly with copepods,water fleas,daphnia,rotifers,ostracods etc. Zooplanktons are microscopic organism normally found in sea ,river,ponds. In Bakerganj and Mathbaria (Bangladesh) the population of zooplanktons were analysed and it was found that Mathbaria\'s river and ponds were actually reservoir for vibrio cholerae. In this areas the environment plays significant role in colonization of zooplankton by pathogenic strains. In these areas the water is used for drinking purposes without any treatment. Cholera is depend on doses.So increase in their numbers increases the chances of endemic or epidemic sometimes pandemics. In these areas flood comes regularly due to which sea water mixes with pond, river water that is also a major factor for the spread of disease. By using molecular genetics and genomic methods for the detection of zooplankton species and determination of selected association of V.cholerae may allow early warning warning of path.