This document discusses viable but non-culturable (VBNC) bacteria. VBNC bacteria are metabolically active but cannot be cultured on standard media. They enter a dormant VBNC state in response to harsh environmental conditions like nutrient starvation, temperature changes, or osmotic stress. While dormant, VBNC bacteria have low metabolic activity but maintain cell integrity and can regain culturability if conditions improve. The document outlines the characteristics of VBNC bacteria and methods to detect them, such as microscopy, gene probes, and resuscitation of dormant cells in more favorable conditions.