SlideShare a Scribd company logo
1 of 24
Instrumenation:
16
 Source of radiation.
 Collimating system.
 Mono-chromator system.
 Sample holder.
 Detector.
 Amplifier and Read-out devise.
Source of radiation
17
 Requirements of an ideal source
 It should be stable and should not allow fluctuations.
 It should emit light of continuous spectrum of high and uniform intensity
over the entire wavelength region in which it’s used.
 It should provide incident light of sufficient intensity for the transmitted
energy to be detected at the end of optic path.
 It should not show fatigue on continued use.
1. Tungsten Halogen Lamp:
 Its construction is similar to a house hold lamp.
 The bulb contains a filament of Tungsten fixed in evacuated condition and
then filled with inert gas.
 The filament can be heated up to 3000 k, beyond this Tungsten starts
sublimating.
 It is used when polychromatic light is required.
DEMERIT:
 It emits the major portion of its radiant energy in near IR region of
the spectrum.
2. Hydrogen Discharge Lamp:
 In Hydrogen discharge lamp pair of electrodes is enclosed in a
glass tube (provided with silica or quartz window for UV radiation
to pass trough) filled with hydrogen gas.
 When current is passed trough these electrodes maintained at high
voltage, discharge of electrons occurs which excites hydrogen
molecules which in turn cause emission of UV radiations in near
UV region.
 They are stable and widely used.
19
3. Xenon Discharge Lamp:
 It possesses two tungsten electrodes separated by some distance.
 These are enclosed in a glass tube (with quartz or fused silica) and
xenon gas is filled under pressure.
 An intense arc is formed between electrodes by applying high
voltage. This is a good source of continuous plus additional
intense radiation. Its intensity is higher than the hydrogen
discharge lamp.
DEMERIT:
 The lamp since operates at high voltage becomes very hot during
operation and hence needs thermal insulation.
20
uv visible spectroscopy in chemistry.pptx
uv visible spectroscopy in chemistry.pptx
uv visible spectroscopy in chemistry.pptx
uv visible spectroscopy in chemistry.pptx
uv visible spectroscopy in chemistry.pptx

More Related Content

Similar to uv visible spectroscopy in chemistry.pptx

What are the working principles of different artificial light sources
What are the working principles of different artificial light sourcesWhat are the working principles of different artificial light sources
What are the working principles of different artificial light sourcesErinChen37
 
Clinical radiation generators
Clinical radiation generatorsClinical radiation generators
Clinical radiation generatorsManish Sandhu
 
1.5 source of artificial light
1.5 source of artificial light1.5 source of artificial light
1.5 source of artificial lightQC Labs
 
Instrumentation of uv visible spectroscopy
Instrumentation of uv visible spectroscopyInstrumentation of uv visible spectroscopy
Instrumentation of uv visible spectroscopyNirali Mistry
 
ELECTRICAL LAMPS AND THEIR TYPES
ELECTRICAL LAMPS AND THEIR TYPESELECTRICAL LAMPS AND THEIR TYPES
ELECTRICAL LAMPS AND THEIR TYPESSAURAV DAYAL SING
 
Ultra Violet Disinfection
Ultra Violet Disinfection Ultra Violet Disinfection
Ultra Violet Disinfection eyestozeoul
 
UV spectroscopy, Electronic transitions, law of UV, Deviations of UV, chromop...
UV spectroscopy, Electronic transitions, law of UV, Deviations of UV, chromop...UV spectroscopy, Electronic transitions, law of UV, Deviations of UV, chromop...
UV spectroscopy, Electronic transitions, law of UV, Deviations of UV, chromop...Rajesh Singh
 
Physics and Operation of Linear Accelerator
Physics and Operation of Linear AcceleratorPhysics and Operation of Linear Accelerator
Physics and Operation of Linear Acceleratorsailakshmi pullookkara
 
Radiation protection course for radiologists L1
Radiation protection course for radiologists L1Radiation protection course for radiologists L1
Radiation protection course for radiologists L1Amin Amin
 
UV Spectroscopy and Its Applications
UV Spectroscopy  and Its ApplicationsUV Spectroscopy  and Its Applications
UV Spectroscopy and Its ApplicationsVikram Choudhary
 
Photomultiplier tube
Photomultiplier tubePhotomultiplier tube
Photomultiplier tubeMoonaRaja2
 
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptx
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptxUV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptx
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptxDr. Vijaya Barge
 
UV-VISIBLE SPECTROSCOPY
UV-VISIBLE SPECTROSCOPYUV-VISIBLE SPECTROSCOPY
UV-VISIBLE SPECTROSCOPYBinuja S.S
 
Production of x rays
Production of x raysProduction of x rays
Production of x raysaslam bs
 

Similar to uv visible spectroscopy in chemistry.pptx (20)

What are the working principles of different artificial light sources
What are the working principles of different artificial light sourcesWhat are the working principles of different artificial light sources
What are the working principles of different artificial light sources
 
U.V Spectroscopy.
U.V Spectroscopy.U.V Spectroscopy.
U.V Spectroscopy.
 
Linear Accelerators.pptx
Linear Accelerators.pptxLinear Accelerators.pptx
Linear Accelerators.pptx
 
Clinical radiation generators
Clinical radiation generatorsClinical radiation generators
Clinical radiation generators
 
Ir instrumentation
Ir  instrumentationIr  instrumentation
Ir instrumentation
 
1.5 source of artificial light
1.5 source of artificial light1.5 source of artificial light
1.5 source of artificial light
 
Instrumentation of uv visible spectroscopy
Instrumentation of uv visible spectroscopyInstrumentation of uv visible spectroscopy
Instrumentation of uv visible spectroscopy
 
ELECTRICAL LAMPS AND THEIR TYPES
ELECTRICAL LAMPS AND THEIR TYPESELECTRICAL LAMPS AND THEIR TYPES
ELECTRICAL LAMPS AND THEIR TYPES
 
Ultra Violet Disinfection
Ultra Violet Disinfection Ultra Violet Disinfection
Ultra Violet Disinfection
 
UV spectroscopy, Electronic transitions, law of UV, Deviations of UV, chromop...
UV spectroscopy, Electronic transitions, law of UV, Deviations of UV, chromop...UV spectroscopy, Electronic transitions, law of UV, Deviations of UV, chromop...
UV spectroscopy, Electronic transitions, law of UV, Deviations of UV, chromop...
 
X-RAY TUBE.pptx
X-RAY TUBE.pptxX-RAY TUBE.pptx
X-RAY TUBE.pptx
 
Physics and Operation of Linear Accelerator
Physics and Operation of Linear AcceleratorPhysics and Operation of Linear Accelerator
Physics and Operation of Linear Accelerator
 
Radiation protection course for radiologists L1
Radiation protection course for radiologists L1Radiation protection course for radiologists L1
Radiation protection course for radiologists L1
 
Neelam
NeelamNeelam
Neelam
 
UV Spectroscopy and Its Applications
UV Spectroscopy  and Its ApplicationsUV Spectroscopy  and Its Applications
UV Spectroscopy and Its Applications
 
Photomultiplier tube
Photomultiplier tubePhotomultiplier tube
Photomultiplier tube
 
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptx
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptxUV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptx
UV-VISIBLE SPECTROSCOPY INSTRUMENTATION.pptx
 
UV-VISIBLE SPECTROSCOPY
UV-VISIBLE SPECTROSCOPYUV-VISIBLE SPECTROSCOPY
UV-VISIBLE SPECTROSCOPY
 
Production of x rays
Production of x raysProduction of x rays
Production of x rays
 
Uv,vis,nmr,mass,ir
Uv,vis,nmr,mass,irUv,vis,nmr,mass,ir
Uv,vis,nmr,mass,ir
 

More from JohamSarfrazAli1

pigments- synthetic dyes and food colors.pptx
pigments- synthetic dyes and food colors.pptxpigments- synthetic dyes and food colors.pptx
pigments- synthetic dyes and food colors.pptxJohamSarfrazAli1
 
General techniques for identification and characterization of protein and Nuc...
General techniques for identification and characterization of protein and Nuc...General techniques for identification and characterization of protein and Nuc...
General techniques for identification and characterization of protein and Nuc...JohamSarfrazAli1
 
Mass Spectrometry in chemistry and basic sciences.pptx
Mass Spectrometry in chemistry and basic sciences.pptxMass Spectrometry in chemistry and basic sciences.pptx
Mass Spectrometry in chemistry and basic sciences.pptxJohamSarfrazAli1
 
Flame atomic emission Spectrometry12.pptx
Flame atomic emission Spectrometry12.pptxFlame atomic emission Spectrometry12.pptx
Flame atomic emission Spectrometry12.pptxJohamSarfrazAli1
 

More from JohamSarfrazAli1 (6)

pigments- synthetic dyes and food colors.pptx
pigments- synthetic dyes and food colors.pptxpigments- synthetic dyes and food colors.pptx
pigments- synthetic dyes and food colors.pptx
 
General techniques for identification and characterization of protein and Nuc...
General techniques for identification and characterization of protein and Nuc...General techniques for identification and characterization of protein and Nuc...
General techniques for identification and characterization of protein and Nuc...
 
Mass Spectrometry in chemistry and basic sciences.pptx
Mass Spectrometry in chemistry and basic sciences.pptxMass Spectrometry in chemistry and basic sciences.pptx
Mass Spectrometry in chemistry and basic sciences.pptx
 
Flame atomic emission Spectrometry12.pptx
Flame atomic emission Spectrometry12.pptxFlame atomic emission Spectrometry12.pptx
Flame atomic emission Spectrometry12.pptx
 
chloroplast.pptx
chloroplast.pptxchloroplast.pptx
chloroplast.pptx
 
cell Membrane.pptx
cell Membrane.pptxcell Membrane.pptx
cell Membrane.pptx
 

Recently uploaded

Recombinant DNA technology (Immunological screening)
Recombinant DNA technology (Immunological screening)Recombinant DNA technology (Immunological screening)
Recombinant DNA technology (Immunological screening)PraveenaKalaiselvan1
 
Presentation Vikram Lander by Vedansh Gupta.pptx
Presentation Vikram Lander by Vedansh Gupta.pptxPresentation Vikram Lander by Vedansh Gupta.pptx
Presentation Vikram Lander by Vedansh Gupta.pptxgindu3009
 
GBSN - Microbiology (Unit 1)
GBSN - Microbiology (Unit 1)GBSN - Microbiology (Unit 1)
GBSN - Microbiology (Unit 1)Areesha Ahmad
 
GBSN - Microbiology (Unit 2)
GBSN - Microbiology (Unit 2)GBSN - Microbiology (Unit 2)
GBSN - Microbiology (Unit 2)Areesha Ahmad
 
TEST BANK For Radiologic Science for Technologists, 12th Edition by Stewart C...
TEST BANK For Radiologic Science for Technologists, 12th Edition by Stewart C...TEST BANK For Radiologic Science for Technologists, 12th Edition by Stewart C...
TEST BANK For Radiologic Science for Technologists, 12th Edition by Stewart C...ssifa0344
 
Zoology 4th semester series (krishna).pdf
Zoology 4th semester series (krishna).pdfZoology 4th semester series (krishna).pdf
Zoology 4th semester series (krishna).pdfSumit Kumar yadav
 
Animal Communication- Auditory and Visual.pptx
Animal Communication- Auditory and Visual.pptxAnimal Communication- Auditory and Visual.pptx
Animal Communication- Auditory and Visual.pptxUmerFayaz5
 
9654467111 Call Girls In Raj Nagar Delhi Short 1500 Night 6000
9654467111 Call Girls In Raj Nagar Delhi Short 1500 Night 60009654467111 Call Girls In Raj Nagar Delhi Short 1500 Night 6000
9654467111 Call Girls In Raj Nagar Delhi Short 1500 Night 6000Sapana Sha
 
Nightside clouds and disequilibrium chemistry on the hot Jupiter WASP-43b
Nightside clouds and disequilibrium chemistry on the hot Jupiter WASP-43bNightside clouds and disequilibrium chemistry on the hot Jupiter WASP-43b
Nightside clouds and disequilibrium chemistry on the hot Jupiter WASP-43bSérgio Sacani
 
fundamental of entomology all in one topics of entomology
fundamental of entomology all in one topics of entomologyfundamental of entomology all in one topics of entomology
fundamental of entomology all in one topics of entomologyDrAnita Sharma
 
Pests of cotton_Sucking_Pests_Dr.UPR.pdf
Pests of cotton_Sucking_Pests_Dr.UPR.pdfPests of cotton_Sucking_Pests_Dr.UPR.pdf
Pests of cotton_Sucking_Pests_Dr.UPR.pdfPirithiRaju
 
Disentangling the origin of chemical differences using GHOST
Disentangling the origin of chemical differences using GHOSTDisentangling the origin of chemical differences using GHOST
Disentangling the origin of chemical differences using GHOSTSérgio Sacani
 
Biopesticide (2).pptx .This slides helps to know the different types of biop...
Biopesticide (2).pptx  .This slides helps to know the different types of biop...Biopesticide (2).pptx  .This slides helps to know the different types of biop...
Biopesticide (2).pptx .This slides helps to know the different types of biop...RohitNehra6
 
Forensic Biology & Its biological significance.pdf
Forensic Biology & Its biological significance.pdfForensic Biology & Its biological significance.pdf
Forensic Biology & Its biological significance.pdfrohankumarsinghrore1
 
❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.
❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.
❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.Nitya salvi
 
Chromatin Structure | EUCHROMATIN | HETEROCHROMATIN
Chromatin Structure | EUCHROMATIN | HETEROCHROMATINChromatin Structure | EUCHROMATIN | HETEROCHROMATIN
Chromatin Structure | EUCHROMATIN | HETEROCHROMATINsankalpkumarsahoo174
 
Hubble Asteroid Hunter III. Physical properties of newly found asteroids
Hubble Asteroid Hunter III. Physical properties of newly found asteroidsHubble Asteroid Hunter III. Physical properties of newly found asteroids
Hubble Asteroid Hunter III. Physical properties of newly found asteroidsSérgio Sacani
 
All-domain Anomaly Resolution Office U.S. Department of Defense (U) Case: “Eg...
All-domain Anomaly Resolution Office U.S. Department of Defense (U) Case: “Eg...All-domain Anomaly Resolution Office U.S. Department of Defense (U) Case: “Eg...
All-domain Anomaly Resolution Office U.S. Department of Defense (U) Case: “Eg...Sérgio Sacani
 
Physiochemical properties of nanomaterials and its nanotoxicity.pptx
Physiochemical properties of nanomaterials and its nanotoxicity.pptxPhysiochemical properties of nanomaterials and its nanotoxicity.pptx
Physiochemical properties of nanomaterials and its nanotoxicity.pptxAArockiyaNisha
 

Recently uploaded (20)

Recombinant DNA technology (Immunological screening)
Recombinant DNA technology (Immunological screening)Recombinant DNA technology (Immunological screening)
Recombinant DNA technology (Immunological screening)
 
Presentation Vikram Lander by Vedansh Gupta.pptx
Presentation Vikram Lander by Vedansh Gupta.pptxPresentation Vikram Lander by Vedansh Gupta.pptx
Presentation Vikram Lander by Vedansh Gupta.pptx
 
GBSN - Microbiology (Unit 1)
GBSN - Microbiology (Unit 1)GBSN - Microbiology (Unit 1)
GBSN - Microbiology (Unit 1)
 
GBSN - Microbiology (Unit 2)
GBSN - Microbiology (Unit 2)GBSN - Microbiology (Unit 2)
GBSN - Microbiology (Unit 2)
 
TEST BANK For Radiologic Science for Technologists, 12th Edition by Stewart C...
TEST BANK For Radiologic Science for Technologists, 12th Edition by Stewart C...TEST BANK For Radiologic Science for Technologists, 12th Edition by Stewart C...
TEST BANK For Radiologic Science for Technologists, 12th Edition by Stewart C...
 
Zoology 4th semester series (krishna).pdf
Zoology 4th semester series (krishna).pdfZoology 4th semester series (krishna).pdf
Zoology 4th semester series (krishna).pdf
 
Animal Communication- Auditory and Visual.pptx
Animal Communication- Auditory and Visual.pptxAnimal Communication- Auditory and Visual.pptx
Animal Communication- Auditory and Visual.pptx
 
9654467111 Call Girls In Raj Nagar Delhi Short 1500 Night 6000
9654467111 Call Girls In Raj Nagar Delhi Short 1500 Night 60009654467111 Call Girls In Raj Nagar Delhi Short 1500 Night 6000
9654467111 Call Girls In Raj Nagar Delhi Short 1500 Night 6000
 
Nightside clouds and disequilibrium chemistry on the hot Jupiter WASP-43b
Nightside clouds and disequilibrium chemistry on the hot Jupiter WASP-43bNightside clouds and disequilibrium chemistry on the hot Jupiter WASP-43b
Nightside clouds and disequilibrium chemistry on the hot Jupiter WASP-43b
 
fundamental of entomology all in one topics of entomology
fundamental of entomology all in one topics of entomologyfundamental of entomology all in one topics of entomology
fundamental of entomology all in one topics of entomology
 
Pests of cotton_Sucking_Pests_Dr.UPR.pdf
Pests of cotton_Sucking_Pests_Dr.UPR.pdfPests of cotton_Sucking_Pests_Dr.UPR.pdf
Pests of cotton_Sucking_Pests_Dr.UPR.pdf
 
Disentangling the origin of chemical differences using GHOST
Disentangling the origin of chemical differences using GHOSTDisentangling the origin of chemical differences using GHOST
Disentangling the origin of chemical differences using GHOST
 
Biopesticide (2).pptx .This slides helps to know the different types of biop...
Biopesticide (2).pptx  .This slides helps to know the different types of biop...Biopesticide (2).pptx  .This slides helps to know the different types of biop...
Biopesticide (2).pptx .This slides helps to know the different types of biop...
 
Forensic Biology & Its biological significance.pdf
Forensic Biology & Its biological significance.pdfForensic Biology & Its biological significance.pdf
Forensic Biology & Its biological significance.pdf
 
❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.
❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.
❤Jammu Kashmir Call Girls 8617697112 Personal Whatsapp Number 💦✅.
 
CELL -Structural and Functional unit of life.pdf
CELL -Structural and Functional unit of life.pdfCELL -Structural and Functional unit of life.pdf
CELL -Structural and Functional unit of life.pdf
 
Chromatin Structure | EUCHROMATIN | HETEROCHROMATIN
Chromatin Structure | EUCHROMATIN | HETEROCHROMATINChromatin Structure | EUCHROMATIN | HETEROCHROMATIN
Chromatin Structure | EUCHROMATIN | HETEROCHROMATIN
 
Hubble Asteroid Hunter III. Physical properties of newly found asteroids
Hubble Asteroid Hunter III. Physical properties of newly found asteroidsHubble Asteroid Hunter III. Physical properties of newly found asteroids
Hubble Asteroid Hunter III. Physical properties of newly found asteroids
 
All-domain Anomaly Resolution Office U.S. Department of Defense (U) Case: “Eg...
All-domain Anomaly Resolution Office U.S. Department of Defense (U) Case: “Eg...All-domain Anomaly Resolution Office U.S. Department of Defense (U) Case: “Eg...
All-domain Anomaly Resolution Office U.S. Department of Defense (U) Case: “Eg...
 
Physiochemical properties of nanomaterials and its nanotoxicity.pptx
Physiochemical properties of nanomaterials and its nanotoxicity.pptxPhysiochemical properties of nanomaterials and its nanotoxicity.pptx
Physiochemical properties of nanomaterials and its nanotoxicity.pptx
 

uv visible spectroscopy in chemistry.pptx

  • 1.
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16. Instrumenation: 16  Source of radiation.  Collimating system.  Mono-chromator system.  Sample holder.  Detector.  Amplifier and Read-out devise.
  • 17. Source of radiation 17  Requirements of an ideal source  It should be stable and should not allow fluctuations.  It should emit light of continuous spectrum of high and uniform intensity over the entire wavelength region in which it’s used.  It should provide incident light of sufficient intensity for the transmitted energy to be detected at the end of optic path.  It should not show fatigue on continued use. 1. Tungsten Halogen Lamp:  Its construction is similar to a house hold lamp.  The bulb contains a filament of Tungsten fixed in evacuated condition and then filled with inert gas.  The filament can be heated up to 3000 k, beyond this Tungsten starts sublimating.  It is used when polychromatic light is required.
  • 18. DEMERIT:  It emits the major portion of its radiant energy in near IR region of the spectrum. 2. Hydrogen Discharge Lamp:  In Hydrogen discharge lamp pair of electrodes is enclosed in a glass tube (provided with silica or quartz window for UV radiation to pass trough) filled with hydrogen gas.  When current is passed trough these electrodes maintained at high voltage, discharge of electrons occurs which excites hydrogen molecules which in turn cause emission of UV radiations in near UV region.  They are stable and widely used. 19
  • 19. 3. Xenon Discharge Lamp:  It possesses two tungsten electrodes separated by some distance.  These are enclosed in a glass tube (with quartz or fused silica) and xenon gas is filled under pressure.  An intense arc is formed between electrodes by applying high voltage. This is a good source of continuous plus additional intense radiation. Its intensity is higher than the hydrogen discharge lamp. DEMERIT:  The lamp since operates at high voltage becomes very hot during operation and hence needs thermal insulation. 20