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The document discusses a university project studying epilepsy in Australian Shepherd dogs. It mentions the University of Minnesota St. Paul Campus LSSURP Project and names Valeria Rivera as the summer 2011 student and Ned Patterson as the mentor. The project focuses on epilepsy in Australian Shepherd dogs.
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Valeria riverafinalpresentationdec2011v11 2
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The study confirms associations between epilepsy in Australian Shepherd dogs and two locations on chromosomes 1 (CFA1) and 19 (CFA19) found in previous research. PCR-RFLP tests on 88 additional dogs showed different genotype frequencies between normal and affected dogs, supporting genes in these locations that may cause epilepsy. Sequencing of the DOK6 gene near associated SNPs on CFA1 found no differences between one affected and unaffected dog. The results support the hypothesis that mutations in these chromosomal regions contribute to epilepsy in Australian Shepherds.
Rivera valeria same
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The document discusses a university project studying epilepsy in Australian Shepherd dogs. It mentions the University of Minnesota St. Paul Campus LSSURP Project and names Valeria Rivera as the summer 2011 student and Ned Patterson as the mentor. The project focuses on epilepsy in Australian Shepherd dogs.
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Campylobacteriosis is a contagious venereal disease of cattle characterized by infertility and repeat breeding. It is caused by the bacteria Campylobacter fetus, which is transmitted during coitus or artificial insemination. Early abortions are common in infected cattle, occurring up to 4 months of gestation. Late abortions can also occasionally occur. The bacteria damage the epithelial lining of the oviduct and uterus, interfering with fertilization and competing with embryos for oxygen, leading to abortions or retained placentas. Diagnosis involves isolating the bacteria, and treatment consists of antibiotics administered both systemically and intrauterinely.
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Coal has the greatest environmental impacts of all energy sources due to its high greenhouse gas emissions, water pollution, and health impacts. Wind and solar have the lowest impacts with minimal greenhouse gas and other air pollutant emissions, though they require large amounts of land. Nuclear power also has relatively low emissions but generates radioactive waste with no long-term storage solution.
Factors influencing carcass composition
Genetic factors, physiological age, sex, nutrition, hormones, and environment all influence the carcass composition of meat animals. Genetic factors like breed can determine the amount of muscle versus fat. Physiological age affects the rates different tissues are deposited. Nutrition impacts the partitioning of nutrients between tissues. Hormones stimulate muscle or fat growth. Environmental stresses can cause animals to utilize energy stores differently. Understanding these factors is important for producing high quality meat.
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- Over 50,000 new biomedical articles are published each year, making it difficult to find relevant information.
- Formulating focused clinical questions using PICO/PIO criteria can help target searches.
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Laura Liscum studies Niemann Pick C (NPC), a lysosomal storage disease where cholesterol does not transport out of the lysosome properly. In NPC cells, the lysosome fills with storage vesicles. Patients experience liver and brain problems, occurring in 1 in 150,000 births. They often die young, around their early teens. One patient, Adam, exhibited neurological symptoms like seizures and lost motor skills. He died at age 10. Most NPC patients have liver issues, as liver cells store large amounts of cholesterol. Liscum hypothesized that excess lipid storage makes lysosomes leaky, activating cell death. Current treatments involve cyclodextrin, which had better results than directly injecting allopregnolone into the
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Synapses are specialized structures that transmit signals between neurons or from neurons to other cells. They convert electrical signals into chemical signals to allow communication between cells by releasing neurotransmitters in response to action potentials. Malfunctions in synaptic transmission can cause disorders like epilepsy, neuropathic pain, anxiety, depression, learning deficits, and dementia. The study focuses on how a calcium channel subunit called a25 that is targeted by anti-epileptic and pain drugs regulates synaptic transmission and structure.
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This study aims to analyze conserved amino acids in GAPDH proteins from tropical plants Oxalis corniculata and Plectranthus amboinicus. GAPDH is important for energy production. Previous work cloned GAPDH genes from these plants and Myrtaceae psidium. Psidium showed no cloning and was eliminated. The current work will sequence the cloned GAPDH inserts and analyze conserved amino acids related to catalytic function through bioinformatics. This adds to knowledge of important plant genes and their evolution.
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This study aims to clone and analyze GAPDH genes from various plant species to compare amino acid sequences related to catalytic function. Previous work successfully cloned GAPDH from Oxalis corniculata and Plectranthus amboinicus but not Myrtaceae psidium. The current study will continue work on the cloned samples through midipreps, restriction enzyme digestion and sequencing. Sequence data will undergo bioinformatics analysis to compare conserved amino acids of the GAPDH protein between plant species. Results could provide new genetic information published in GenBank and further the understanding of energy production pathways in plants.
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This document summarizes a study on cloning and analyzing glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes from different plant species. The researcher amplified GAPDH genes from Oxalis corniculata, Plectranthus amboinicus, and Myrtaceae psidium via PCR. They then cloned the genes into E. coli and sequenced them using Sanger sequencing. The goal was to compare the GAPDH sequences to analyze conserved amino acids in the active site and differences elsewhere, helping understand GAPDH protein function and evolution across plants. Preliminary results were obtained but not detailed.
Valeria riverafinalpresentationdec2011v11 2
The author cloned GAPDH genes from Oxalis corniculata and Plectranthus amboinicus plants and conducted bioinformatics analyses. GAPDH is important for energy production. The genes were amplified by PCR and sequenced. Sequence analyses showed the cloned genes were GAPC from O. corniculata and GAPDH from P. amboinicus. Future work will include more detailed bioinformatics analyses and submitting the novel sequences to GenBank.
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The study confirms associations between epilepsy in Australian Shepherd dogs and two locations on chromosomes 1 (CFA1) and 19 (CFA19) found in previous research. PCR-RFLP tests on 88 additional dogs showed different genotype frequencies between normal and affected dogs, supporting genes in these locations that may cause epilepsy. Sequencing of the DOK6 gene near associated SNPs on CFA1 found no differences between one affected and unaffected dog. The results support the hypothesis that mutations in these chromosomal regions contribute to epilepsy in Australian Shepherds.
Rivera valeria same
This study aimed to confirm previous findings linking canine chromosomes 1 and 19 to idiopathic epilepsy in Australian Shepherd dogs. PCR-RFLP tests of two SNPs were performed on 88 additional Australian Shepherds and genotypes were compared between affected and unaffected dogs via Chi-square analysis. Sequencing of the DOK6 gene did not reveal differences between one affected and unaffected dog. The results confirmed different genotype frequencies between normal and affected dogs for the SNPs, supporting the hypothesis that epilepsy-causing genes are located near the tested regions on chromosomes 1 and 19.
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This document summarizes research being done to identify genetic factors that cause epilepsy in Australian Shepherd dogs. The researchers are genotyping additional dogs to confirm associations between epilepsy and SNPs on chromosomes 1 and 19 found in previous work. They plan to sequence the DOK6 gene near one of the SNPs on chromosome 1 to look for mutations correlated with epilepsy. Current work includes genotyping 80 additional dogs and sequencing exons of the DOK6 gene, though one exon is still being analyzed. The goal is to identify the specific genetic cause of epilepsy in this breed to improve treatment and prevent transmission.
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This lab used an ELISA test to simulate the sharing of bodily fluids and determine which students were exposed to and transmitted a contagious disease. The ELISA test relies on antibodies to detect antigens produced during an immune response to exposure. The results showed students 1, 2, 8, and 10 were infected, while students 1 and 10 transmitted the disease. ELISA is commonly used in real world applications such as pregnancy tests, disease detection, and testing for food safety and GMOs.
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The document announces a nanotechnology workshop from October 7th to 9th organized by Ms. Ivonne Ferrer and Ms. Jesus Velazquez. The workshop will likely focus on nanotechnology topics over the three day period and is being run by the two individuals mentioned.
University of minnesota blog photos
The document discusses a university project studying epilepsy in Australian Shepherd dogs. It mentions the University of Minnesota St. Paul Campus LSSURP Project and names Valeria Rivera as the summer 2011 student and Ned Patterson as the mentor. The project focuses on epilepsy in Australian Shepherd dogs.
Biol 3009 resumenes
This document summarizes the speaker's experience in three biology courses at college. In BIOL 3009, the speaker learned about the central dogma of biology including DNA replication, transcription of DNA to RNA, and translation of RNA to proteins. They enjoyed conducting a laboratory experiment to identify fruits containing enzymes. In BIOL 3095, the speaker learned to write scientific papers and presented a review paper on osteoarthritis treatments. They attended seminars by alumni researchers. In BIOL 3097, the speaker dissected samples under microscopes, extracted their own DNA, and conducted research with a mentor on GMOs, presenting their findings. They viewed the hands-on experiences as unique and fun opportunities to learn.
Reflective essay blog
The student reflects on their experience in the RISE program at UPR-Cayey. They took biology courses where they conducted laboratory work and wrote proposals. This helped them think like a scientist by asking questions and searching for information. They attended RISE seminars that introduced them to new scientific terms and potential research opportunities. This led the student to be selected for a summer research program at the University of Minnesota where they wrote annotated bibliographies and learned to communicate scientific findings. In their second semester, the RISE biomedical techniques course taught them new lab techniques to help achieve their career goals in the sciences.
Lab 12 e
This lab used an ELISA test to simulate the sharing of a contagious disease between students and determine who was infected and who transmitted the disease. The ELISA test relies on antibodies binding to antigens produced during an immune response to detect exposure to a disease in liquid samples. The results of the lab showed that students 1, 2, 8, and 10 were infected, while students 1 and 10 transmitted the disease to others.
Lab 11 e
Beer's law states that absorbance is directly proportional to concentration, allowing this lab to use a spectrophotometer to calculate protein levels in milk samples. The students prepared 1:50 dilutions of evaporated and fresh milk with dye, incubated for 40 minutes, and measured absorbance at 595 wavelengths to generate a standard curve and determine that sample A, evaporated milk, had a higher protein concentration than the fresh milk.
Lab 10 e
Enzymes are proteins that act as catalysts and have specific shapes that determine their substrates and products. The experiment determined the optimum temperature of the enzyme cellobiase, which breaks down cellulose, to be 22°C. Samples of cellobiase were tested at 0°, 22°, and 37°C using a spectrophotometer to measure absorbance, finding that the enzyme works best at 22°C and its activity is minimized at higher temperatures due to denaturing.
Lab 9 e
During a lab experiment, students created a hydrogen sensor using palladium-coated polymer nanofibers produced through electrospinning and sputter coating, which was then tested in a chamber with controlled hydrogen and argon gas flows. The sensor worked effectively, with an increase in current detected as hydrogen levels rose, demonstrating an application of nanotechnology to increase surface area and catalytic reactivity for hydrogen detection.
Autobiography val
Valeria Rivera was born on December 17, 1992 in San Juan, Puerto Rico weighing 5 pounds and 10 ounces. She enjoys arts and crafts, knitting, bicycling, jogging, visiting beaches and swimming. She graduated high school with high honors in 2010 and is interested in studying veterinary medicine, having done volunteer work at a veterinary clinic.
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LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UP
This Dissertation explores the particular circumstances of Mirzapur, a region located in the
core of India. Mirzapur, with its varied terrains and abundant biodiversity, offers an optimal
environment for investigating the changes in vegetation cover dynamics. Our study utilizes
advanced technologies such as GIS (Geographic Information Systems) and Remote sensing to
analyze the transformations that have taken place over the course of a decade.
The complex relationship between human activities and the environment has been the focus
of extensive research and worry. As the global community grapples with swift urbanization,
population expansion, and economic progress, the effects on natural ecosystems are becoming
more evident. A crucial element of this impact is the alteration of vegetation cover, which plays a
significant role in maintaining the ecological equilibrium of our planet.Land serves as the foundation for all human activities and provides the necessary materials for
these activities. As the most crucial natural resource, its utilization by humans results in different
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The utilization of land is impacted by human needs and environmental factors. In countries
like India, rapid population growth and the emphasis on extensive resource exploitation can lead
to significant land degradation, adversely affecting the region's land cover.
Therefore, human intervention has significantly influenced land use patterns over many
centuries, evolving its structure over time and space. In the present era, these changes have
accelerated due to factors such as agriculture and urbanization. Information regarding land use and
cover is essential for various planning and management tasks related to the Earth's surface,
providing crucial environmental data for scientific, resource management, policy purposes, and
diverse human activities.
Accurate understanding of land use and cover is imperative for the development planning
of any area. Consequently, a wide range of professionals, including earth system scientists, land
and water managers, and urban planners, are interested in obtaining data on land use and cover
changes, conversion trends, and other related patterns. The spatial dimensions of land use and
cover support policymakers and scientists in making well-informed decisions, as alterations in
these patterns indicate shifts in economic and social conditions. Monitoring such changes with the
help of Advanced technologies like Remote Sensing and Geographic Information Systems is
crucial for coordinated efforts across different administrative levels. Advanced technologies like
Remote Sensing and Geographic Information Systems
9
Changes in vegetation cover refer to variations in the distribution, composition, and overall
structure of plant communities across different temporal and spatial scales. These changes can
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