UV-visible spectroscopy responds to electronic transitions involving pi and lone pair orbitals and is used to identify conjugated systems which tend to have stronger absorption. There is no single light source for the entire UV and visible region, with tungsten lamps used for the visible region and deuterium lamps for the ultraviolet region. Samples are typically held in quartz due to absorption of UV by glass, while solvents must be transparent in the region being observed and commonly lack conjugated systems or carbonyls. UV-visible spectroscopy can be used in the oils industry to detect trans isomers, determine purity, and estimate components like oryzanol, gossypol, and chlorophyll.