SlideShare a Scribd company logo
Ultraviolet & visible
spectroscopy
UV-VISIBLE SPECTROSCOPY RESPONDS TO TRANSITION
INVOLVING PIE AND LONE PAIR ORBITALS & IS USED FOR
IDENTIFYING CONJUGATED SYSTEMS WHICH TENDS TO HAVE
STRONGER ABSORPTION.
INSTRUMENTATION
SOURCE OF LIGHT FOR UV-VIS




 THERE IS NO SINGLE LIGHT SOURCE FOR THE ENTIRE
  UV & VISIBLE REGION.
 TUNGSTEN LAMP-VISIBLE REGION
 DEUTERIUM LAMP-ULTRAVOILET REGION.
SAMPLE HOLDER &
          MONOCHROMATOR


 SAMPLE HOLDER IS GENERALLY MADE UP OF QUARTZ
  CRYSTAL BECAUSE GLASS DO ABSORBS AT UV REGION.
 HOWEVER GLASS HOLDER FOR SCANNING IN UV
  REGION.
 MONCHROMATOR IS GENERALLY GRAFTER OR
  ROTATING PRISM.
 PHOTODIODE ARRAYS ARE NOW AVAILABLE WHICH
  PRODUCES ACCURATE WAVELENGTH.
SOLVENT SELECTION

Solvents must be transparent in the region to
be observed; the wavelength where a solvent is
no longer transparent is referred to as the
cutoff


Since spectra are only obtained up to 200 nm,
solvents typically only need to lack conjugated
  systems or carbonyls
SOLVENT SELECTION
 Common solvents and cutoffs:
   acetonitrile 190
   chloroform 240
   cyclohexane 195
   1,4-dioxane 215
   95% ethanol205
   n-hexane     201
   methanol     205
   isooctane    195
   water        190
APPLICATION IN OILS INDUSTRY


 UV IS TO HPLC WHAT MS IS TO GC.
 UV VIS ABSORPTION SPECTROSCOPY CAN BE USED TO
  DETECT TRANS ISOMER SINCE TRANS ABSORBS .
 USED TO DETERMINE PURITY OF THE SAMPLE.
 ESTIMATION OF CONJUGATED ISOMERS IN ALKALI
  ISOMERISED LINSEED OIL.
 ESTIMATION OF ALPHA & BETA ELEOSTEARIC ACID IN
  TUNG OIL
APPLICATION IN OILS INDUSTRY



 DETERMINATION OF ORYZANOL CONTENT IN RICE
  BRAN OIL.
 ESTIMATION OF GOSSYPOL IN COTTONSEED OIL.
 ESTIMATION OF CHLOROPHYLL IN OIL
 ESTIMATION OF PHOSPHORUS CONTENT

More Related Content

Viewers also liked

Uv visible spectroscopy- madan
Uv visible spectroscopy- madanUv visible spectroscopy- madan
Uv visible spectroscopy- madan
Madan Sigdel
 
UV-module 4
UV-module 4UV-module 4
UV-module 4
Obinna Ukah
 
Ch08
Ch08Ch08
Ch08
klivsie
 
Applications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
Applications of IR (Infrared) Spectroscopy in Pharmaceutical IndustryApplications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
Applications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
wonderingsoul114
 
UV-Visible Spectroscopy
UV-Visible SpectroscopyUV-Visible Spectroscopy
UV-Visible Spectroscopy
Sudha Reddy
 
Application of u.v. spectroscopy
Application of u.v. spectroscopyApplication of u.v. spectroscopy
Application of u.v. spectroscopy
Manish Kumar
 

Viewers also liked (6)

Uv visible spectroscopy- madan
Uv visible spectroscopy- madanUv visible spectroscopy- madan
Uv visible spectroscopy- madan
 
UV-module 4
UV-module 4UV-module 4
UV-module 4
 
Ch08
Ch08Ch08
Ch08
 
Applications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
Applications of IR (Infrared) Spectroscopy in Pharmaceutical IndustryApplications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
Applications of IR (Infrared) Spectroscopy in Pharmaceutical Industry
 
UV-Visible Spectroscopy
UV-Visible SpectroscopyUV-Visible Spectroscopy
UV-Visible Spectroscopy
 
Application of u.v. spectroscopy
Application of u.v. spectroscopyApplication of u.v. spectroscopy
Application of u.v. spectroscopy
 

Similar to Ultraviolet & visible spectroscopy

Oil in water analyzer
Oil in water analyzerOil in water analyzer
Oil in water analyzer
Mohamed Sarhan
 
Flash cromatography
Flash cromatographyFlash cromatography
Flash cromatography
Sandeep Sahu
 
Wavelength Accuracy.pptx
Wavelength Accuracy.pptxWavelength Accuracy.pptx
Wavelength Accuracy.pptx
sumitdekate1
 
Calibration of spectrophotometer
Calibration of spectrophotometerCalibration of spectrophotometer
Calibration of spectrophotometer
Deepak Shilkar
 
phyto chemical finger printing in phytochemistry
phyto chemical finger printing in phytochemistryphyto chemical finger printing in phytochemistry
phyto chemical finger printing in phytochemistry
ssuser22bccd
 
The Quantification of Oligonucleotide using a LAMBDA 465 UV/Vis Spectrophotom...
The Quantification of Oligonucleotide using a LAMBDA 465 UV/Vis Spectrophotom...The Quantification of Oligonucleotide using a LAMBDA 465 UV/Vis Spectrophotom...
The Quantification of Oligonucleotide using a LAMBDA 465 UV/Vis Spectrophotom...
PerkinElmer, Inc.
 
Calibration of analytical instruments
Calibration of analytical instrumentsCalibration of analytical instruments
Calibration of analytical instruments
VigneshVicky470
 
Combined Atomic Absorptoin Spectroscopy
Combined Atomic Absorptoin SpectroscopyCombined Atomic Absorptoin Spectroscopy
Combined Atomic Absorptoin Spectroscopy
knowledge1995
 

Similar to Ultraviolet & visible spectroscopy (8)

Oil in water analyzer
Oil in water analyzerOil in water analyzer
Oil in water analyzer
 
Flash cromatography
Flash cromatographyFlash cromatography
Flash cromatography
 
Wavelength Accuracy.pptx
Wavelength Accuracy.pptxWavelength Accuracy.pptx
Wavelength Accuracy.pptx
 
Calibration of spectrophotometer
Calibration of spectrophotometerCalibration of spectrophotometer
Calibration of spectrophotometer
 
phyto chemical finger printing in phytochemistry
phyto chemical finger printing in phytochemistryphyto chemical finger printing in phytochemistry
phyto chemical finger printing in phytochemistry
 
The Quantification of Oligonucleotide using a LAMBDA 465 UV/Vis Spectrophotom...
The Quantification of Oligonucleotide using a LAMBDA 465 UV/Vis Spectrophotom...The Quantification of Oligonucleotide using a LAMBDA 465 UV/Vis Spectrophotom...
The Quantification of Oligonucleotide using a LAMBDA 465 UV/Vis Spectrophotom...
 
Calibration of analytical instruments
Calibration of analytical instrumentsCalibration of analytical instruments
Calibration of analytical instruments
 
Combined Atomic Absorptoin Spectroscopy
Combined Atomic Absorptoin SpectroscopyCombined Atomic Absorptoin Spectroscopy
Combined Atomic Absorptoin Spectroscopy
 

More from Guruprasad Rao

Methyl ester
Methyl esterMethyl ester
Methyl ester
Guruprasad Rao
 
Single cell oil production
Single cell oil productionSingle cell oil production
Single cell oil production
Guruprasad Rao
 
Deodorization
DeodorizationDeodorization
Deodorization
Guruprasad Rao
 
falling film sulphonation
falling film sulphonationfalling film sulphonation
falling film sulphonation
Guruprasad Rao
 
Fragrances in soap
Fragrances in soapFragrances in soap
Fragrances in soap
Guruprasad Rao
 
High performance(pressure
High performance(pressureHigh performance(pressure
High performance(pressure
Guruprasad Rao
 
Mass spectroscopy
Mass spectroscopyMass spectroscopy
Mass spectroscopy
Guruprasad Rao
 
Thin layer chromatography
Thin layer chromatographyThin layer chromatography
Thin layer chromatography
Guruprasad Rao
 
Health hazards of fragnance
Health hazards of fragnanceHealth hazards of fragnance
Health hazards of fragnance
Guruprasad Rao
 
Shaving cream
Shaving creamShaving cream
Shaving cream
Guruprasad Rao
 

More from Guruprasad Rao (10)

Methyl ester
Methyl esterMethyl ester
Methyl ester
 
Single cell oil production
Single cell oil productionSingle cell oil production
Single cell oil production
 
Deodorization
DeodorizationDeodorization
Deodorization
 
falling film sulphonation
falling film sulphonationfalling film sulphonation
falling film sulphonation
 
Fragrances in soap
Fragrances in soapFragrances in soap
Fragrances in soap
 
High performance(pressure
High performance(pressureHigh performance(pressure
High performance(pressure
 
Mass spectroscopy
Mass spectroscopyMass spectroscopy
Mass spectroscopy
 
Thin layer chromatography
Thin layer chromatographyThin layer chromatography
Thin layer chromatography
 
Health hazards of fragnance
Health hazards of fragnanceHealth hazards of fragnance
Health hazards of fragnance
 
Shaving cream
Shaving creamShaving cream
Shaving cream
 

Recently uploaded

UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5
DianaGray10
 
Presentation of the OECD Artificial Intelligence Review of Germany
Presentation of the OECD Artificial Intelligence Review of GermanyPresentation of the OECD Artificial Intelligence Review of Germany
Presentation of the OECD Artificial Intelligence Review of Germany
innovationoecd
 
Pushing the limits of ePRTC: 100ns holdover for 100 days
Pushing the limits of ePRTC: 100ns holdover for 100 daysPushing the limits of ePRTC: 100ns holdover for 100 days
Pushing the limits of ePRTC: 100ns holdover for 100 days
Adtran
 
PCI PIN Basics Webinar from the Controlcase Team
PCI PIN Basics Webinar from the Controlcase TeamPCI PIN Basics Webinar from the Controlcase Team
PCI PIN Basics Webinar from the Controlcase Team
ControlCase
 
TrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy SurveyTrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy Survey
TrustArc
 
20240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 202420240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 2024
Matthew Sinclair
 
Full-RAG: A modern architecture for hyper-personalization
Full-RAG: A modern architecture for hyper-personalizationFull-RAG: A modern architecture for hyper-personalization
Full-RAG: A modern architecture for hyper-personalization
Zilliz
 
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
名前 です男
 
Enchancing adoption of Open Source Libraries. A case study on Albumentations.AI
Enchancing adoption of Open Source Libraries. A case study on Albumentations.AIEnchancing adoption of Open Source Libraries. A case study on Albumentations.AI
Enchancing adoption of Open Source Libraries. A case study on Albumentations.AI
Vladimir Iglovikov, Ph.D.
 
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdf
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdfObservability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdf
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdf
Paige Cruz
 
UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6
DianaGray10
 
Large Language Model (LLM) and it’s Geospatial Applications
Large Language Model (LLM) and it’s Geospatial ApplicationsLarge Language Model (LLM) and it’s Geospatial Applications
Large Language Model (LLM) and it’s Geospatial Applications
Rohit Gautam
 
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdf
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdfUnlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdf
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdf
Malak Abu Hammad
 
GraphSummit Singapore | Graphing Success: Revolutionising Organisational Stru...
GraphSummit Singapore | Graphing Success: Revolutionising Organisational Stru...GraphSummit Singapore | Graphing Success: Revolutionising Organisational Stru...
GraphSummit Singapore | Graphing Success: Revolutionising Organisational Stru...
Neo4j
 
Monitoring Java Application Security with JDK Tools and JFR Events
Monitoring Java Application Security with JDK Tools and JFR EventsMonitoring Java Application Security with JDK Tools and JFR Events
Monitoring Java Application Security with JDK Tools and JFR Events
Ana-Maria Mihalceanu
 
Climate Impact of Software Testing at Nordic Testing Days
Climate Impact of Software Testing at Nordic Testing DaysClimate Impact of Software Testing at Nordic Testing Days
Climate Impact of Software Testing at Nordic Testing Days
Kari Kakkonen
 
Building RAG with self-deployed Milvus vector database and Snowpark Container...
Building RAG with self-deployed Milvus vector database and Snowpark Container...Building RAG with self-deployed Milvus vector database and Snowpark Container...
Building RAG with self-deployed Milvus vector database and Snowpark Container...
Zilliz
 
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
SOFTTECHHUB
 
Removing Uninteresting Bytes in Software Fuzzing
Removing Uninteresting Bytes in Software FuzzingRemoving Uninteresting Bytes in Software Fuzzing
Removing Uninteresting Bytes in Software Fuzzing
Aftab Hussain
 
Securing your Kubernetes cluster_ a step-by-step guide to success !
Securing your Kubernetes cluster_ a step-by-step guide to success !Securing your Kubernetes cluster_ a step-by-step guide to success !
Securing your Kubernetes cluster_ a step-by-step guide to success !
KatiaHIMEUR1
 

Recently uploaded (20)

UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5UiPath Test Automation using UiPath Test Suite series, part 5
UiPath Test Automation using UiPath Test Suite series, part 5
 
Presentation of the OECD Artificial Intelligence Review of Germany
Presentation of the OECD Artificial Intelligence Review of GermanyPresentation of the OECD Artificial Intelligence Review of Germany
Presentation of the OECD Artificial Intelligence Review of Germany
 
Pushing the limits of ePRTC: 100ns holdover for 100 days
Pushing the limits of ePRTC: 100ns holdover for 100 daysPushing the limits of ePRTC: 100ns holdover for 100 days
Pushing the limits of ePRTC: 100ns holdover for 100 days
 
PCI PIN Basics Webinar from the Controlcase Team
PCI PIN Basics Webinar from the Controlcase TeamPCI PIN Basics Webinar from the Controlcase Team
PCI PIN Basics Webinar from the Controlcase Team
 
TrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy SurveyTrustArc Webinar - 2024 Global Privacy Survey
TrustArc Webinar - 2024 Global Privacy Survey
 
20240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 202420240607 QFM018 Elixir Reading List May 2024
20240607 QFM018 Elixir Reading List May 2024
 
Full-RAG: A modern architecture for hyper-personalization
Full-RAG: A modern architecture for hyper-personalizationFull-RAG: A modern architecture for hyper-personalization
Full-RAG: A modern architecture for hyper-personalization
 
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
みなさんこんにちはこれ何文字まで入るの?40文字以下不可とか本当に意味わからないけどこれ限界文字数書いてないからマジでやばい文字数いけるんじゃないの?えこ...
 
Enchancing adoption of Open Source Libraries. A case study on Albumentations.AI
Enchancing adoption of Open Source Libraries. A case study on Albumentations.AIEnchancing adoption of Open Source Libraries. A case study on Albumentations.AI
Enchancing adoption of Open Source Libraries. A case study on Albumentations.AI
 
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdf
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdfObservability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdf
Observability Concepts EVERY Developer Should Know -- DeveloperWeek Europe.pdf
 
UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6UiPath Test Automation using UiPath Test Suite series, part 6
UiPath Test Automation using UiPath Test Suite series, part 6
 
Large Language Model (LLM) and it’s Geospatial Applications
Large Language Model (LLM) and it’s Geospatial ApplicationsLarge Language Model (LLM) and it’s Geospatial Applications
Large Language Model (LLM) and it’s Geospatial Applications
 
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdf
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdfUnlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdf
Unlock the Future of Search with MongoDB Atlas_ Vector Search Unleashed.pdf
 
GraphSummit Singapore | Graphing Success: Revolutionising Organisational Stru...
GraphSummit Singapore | Graphing Success: Revolutionising Organisational Stru...GraphSummit Singapore | Graphing Success: Revolutionising Organisational Stru...
GraphSummit Singapore | Graphing Success: Revolutionising Organisational Stru...
 
Monitoring Java Application Security with JDK Tools and JFR Events
Monitoring Java Application Security with JDK Tools and JFR EventsMonitoring Java Application Security with JDK Tools and JFR Events
Monitoring Java Application Security with JDK Tools and JFR Events
 
Climate Impact of Software Testing at Nordic Testing Days
Climate Impact of Software Testing at Nordic Testing DaysClimate Impact of Software Testing at Nordic Testing Days
Climate Impact of Software Testing at Nordic Testing Days
 
Building RAG with self-deployed Milvus vector database and Snowpark Container...
Building RAG with self-deployed Milvus vector database and Snowpark Container...Building RAG with self-deployed Milvus vector database and Snowpark Container...
Building RAG with self-deployed Milvus vector database and Snowpark Container...
 
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!
 
Removing Uninteresting Bytes in Software Fuzzing
Removing Uninteresting Bytes in Software FuzzingRemoving Uninteresting Bytes in Software Fuzzing
Removing Uninteresting Bytes in Software Fuzzing
 
Securing your Kubernetes cluster_ a step-by-step guide to success !
Securing your Kubernetes cluster_ a step-by-step guide to success !Securing your Kubernetes cluster_ a step-by-step guide to success !
Securing your Kubernetes cluster_ a step-by-step guide to success !
 

Ultraviolet & visible spectroscopy

  • 1. Ultraviolet & visible spectroscopy UV-VISIBLE SPECTROSCOPY RESPONDS TO TRANSITION INVOLVING PIE AND LONE PAIR ORBITALS & IS USED FOR IDENTIFYING CONJUGATED SYSTEMS WHICH TENDS TO HAVE STRONGER ABSORPTION.
  • 3. SOURCE OF LIGHT FOR UV-VIS  THERE IS NO SINGLE LIGHT SOURCE FOR THE ENTIRE UV & VISIBLE REGION.  TUNGSTEN LAMP-VISIBLE REGION  DEUTERIUM LAMP-ULTRAVOILET REGION.
  • 4. SAMPLE HOLDER & MONOCHROMATOR  SAMPLE HOLDER IS GENERALLY MADE UP OF QUARTZ CRYSTAL BECAUSE GLASS DO ABSORBS AT UV REGION.  HOWEVER GLASS HOLDER FOR SCANNING IN UV REGION.  MONCHROMATOR IS GENERALLY GRAFTER OR ROTATING PRISM.  PHOTODIODE ARRAYS ARE NOW AVAILABLE WHICH PRODUCES ACCURATE WAVELENGTH.
  • 5. SOLVENT SELECTION Solvents must be transparent in the region to be observed; the wavelength where a solvent is no longer transparent is referred to as the cutoff Since spectra are only obtained up to 200 nm, solvents typically only need to lack conjugated systems or carbonyls
  • 6. SOLVENT SELECTION  Common solvents and cutoffs:  acetonitrile 190  chloroform 240  cyclohexane 195  1,4-dioxane 215  95% ethanol205  n-hexane 201  methanol 205  isooctane 195  water 190
  • 7. APPLICATION IN OILS INDUSTRY  UV IS TO HPLC WHAT MS IS TO GC.  UV VIS ABSORPTION SPECTROSCOPY CAN BE USED TO DETECT TRANS ISOMER SINCE TRANS ABSORBS .  USED TO DETERMINE PURITY OF THE SAMPLE.  ESTIMATION OF CONJUGATED ISOMERS IN ALKALI ISOMERISED LINSEED OIL.  ESTIMATION OF ALPHA & BETA ELEOSTEARIC ACID IN TUNG OIL
  • 8. APPLICATION IN OILS INDUSTRY  DETERMINATION OF ORYZANOL CONTENT IN RICE BRAN OIL.  ESTIMATION OF GOSSYPOL IN COTTONSEED OIL.  ESTIMATION OF CHLOROPHYLL IN OIL  ESTIMATION OF PHOSPHORUS CONTENT