tube1 tube4 tube5 tube7 17.670000000000002 16.670000000000002 9.67 26.67 Tube Average Time (min) Harris 1 Drosophila Melanogaster Fly To determination which part of the fly thorax cell homogenate subcellular site of glycolysis and respiration. Biology 155 April 5, 2016 Erricul Harris Hunter Hannegan Moriah Trujillo Logan Brown Table of Contents 2Abstract 2Introduction 2Cell Fractionation 3Methods 3Part A: Preparation of homogenate 6Part B: Biochemical analysis of fractionated homogenate 7Results and Analysis 11Discussion and conclusion 13References Abstract This experiment is performed to demonstrate which part of the fly thorax cell homogenate and carries out glycolysis and which part carries out respiration. More importantly, it was based on good knowledge of living cells of the flies which is useful in discussing the action of glycolysis and carrying out of respiration in the body of a fly. The lab results were then obtained by collecting the flies whose body parts were used to test the assumption that the mitochondrion cells are found in the thorax. Color observations were recorded to determine the usage of oxygen and therefore showing respiration took place. It was observed that glucose was used fast in the test tubes used for the experiment. It was therefore concluded that respiration takes place in the thorax of flies. This experiment is significant in finding out the location of cells used for respiration in flies. Introduction Cell Fractionation There are two major pieces of evidence that give us the details of the process an intercellular process; (1) evidence obtained from biological and physical separation of intercellular constituents actual (2) inferences obtained through observation by an aid of a microscope from microscopic observation(Keeton,1976 n.d.). The experiment aims at provision of evidence for localization of the cellular respiration of glycolysis and that of mitochondria as cytoplasm soluble; hence, verify the various functions of particular cell parts. In the experiment, we employed two methods on my research: Centrifugation and homogenization. The material was spun in a centrifuge machine on its axis where centrifugal force caused the heavy stuff (suspension) directed the axis outwards. The suspension moved and collected at the end of the centrifuge tube. The choice of experimental material is the need to obtain a high number of respiratory and glycolytic and separability of mitochondria from the soluble hence my selection of insect flight muscle. The hypothesis (H0) is that there is an increase of glycolysis and respiration enzymes in cells, whereas the alternate hypothesis (H1) is that there is no significant respiration process relation glycolysis and respiration in insect wing.Methods Part A: Preparation of homogenate The clean homogenizer was chilled in ice bath f ...