TROPISMS
 the movement is always
a growth movement
are responses by part of a plant
towards or away from a stimulus
coming from one direction
Tropic responses can be:
Negative –
if growth is away from
the stimulus
Positive –
if growth is towards
the stimulus
Gravity
Growth
Growth
Phototropism
is a growth
response to light
Light
Geotropism
is a growth response
to gravity
A shoot is:
POSITIVELY
PHOTOTROPIC
NEGATIVELY
GEOTROPIC
Advantages of a shoot growing:
 Towards light:
 To trap more light for photosynthesis
 Away from gravity:
 shoot of a germinating seed grows out of
the soil
 more chances for pollination & seed
dispersal
A root is:
 POSITIVELY GEOTROPIC
 NEGATIVELY PHOTOTROPIC
I am growing
towards gravity,
but where is the
water????
Advantages of a root growing towards
gravity:
 To anchor the plant
 To search for water
 To ensure that the root of a
germinating seed always grows
downwards, whatever, the position
of the seed in the soil
Study the pictures below and then complete the
table by putting a plus (+) if the shoot or root
grows towards the stimulus and a minus (-) if it
grows away from it.
Stimulus
Light Gravity
Shoot
Root
Study the pictures below and then complete the
table by putting a plus (+) if the shoot or root
grows towards the stimulus and a minus (-) if it
grows away from it.
Stimulus
Light Gravity
Shoot + -
Root - +
ANSWER THE FOLLOWING
Study the diagrams. What would you
expect to happen and why.
The shoot with the covered tip grows (straight
up / towards light) because it (can sense light /
cannot sense light).
Study the diagrams. What would you
expect to happen and why.
The shoot with the covered tip grows (straight
up / towards light) because it (can sense light /
cannot sense light).
The shoot with the exposed tip grows (straight
up / towards light) because it (can sense light /
cannot sense light).
The shoot with the exposed tip grows (straight
up / towards light) because it (can sense light /
cannot sense light).
Look at the diagrams below. What does this
experiment tell you about the tip of the shoot
and the part it plays in growth?
Tip produces chemicals for growth.
Look at the diagrams below. How would you
explain the results of this experiment?
Bending resulted due to unequal amount of
chemical . Light affects distribution of chemical.
AUXINS
Auxins
• are plant hormones, or growth regulating
chemicals produced in the root and shoot
tips that affect growth
What is the effect of auxins on plant cells?
Cells absorb water and elongate.
Three regions of a root tip
Cell division region
Cell
differentiation
region
Cell elongation
region
[Cells become specialised]
Which region does auxin affect?
Cell division region
(root tip that makes auxin)
Cell differentiation region
(cells change their shape;
no effect by auxin)
Cell elongation region
(auxin makes cells absorb water)
Explain why:
markings have
moved away from
each other ONLY in
the lower region.
Cell elongation
region
A shoot growing in even illumination grows
straight up. Why?
auxin
Cells on both sides of the
stem elongate equally.
A shoot growing in one-sided illumination.
A shoot growing in one-sided illumination
grows towards light. How is it possible?
Cells on the two
sides of the stem
GROW UNEQUALLY!!
Remember: UNEQUAL growth of stem
sides results in BENDING of stem
Lighted side:
normal size of cells
Shaded side of stem:
cells elongate
Light
Describe how a shoot bends towards
one-sided illumination.
Auxins are produced at the ___________ tip and
diffuse down the stem.
More auxin collects on the ____________ side of
the stem.
Auxins cause the cells to absorb water and so
elongate in the cell elongation region.
Thus the ___________ side grows more than the
lighted one.
This results in bending of the stem towards light.
shoot
shaded
shaded
Auxins sometimes stop growth
Auxin passes down the stem
So the plant grows tall and straight.
1.
Apical bud produces auxin.
2.
Lateral buds do not grow into
side branches.
3.
If the top is cut off from the
plant:
 flow of auxin stops
 side branches will grow
out
Sometimes gardeners cut the tops off plants to
make them more bushy
QUESTION: MAY, 2009
Give a biological explanation for each of the
following statements:
cutting off the tip of a shoot will stop the shoot
from getting taller but it will increase
bushiness; (3)
Cutting off the tip of a
shoot removes the
source of auxin.
Lack of auxin stimulates
lateral buds to develop
and side branches grow.
EXPERIMENTS TO SHOW:
1. Geotropism in a shoot
2. Geotropism in pea radicles
3. Phototropism in oat coleoptiles
4. Phototropism in a shoot
All make
use of an
apparatus
called:
clinostat
A motor that rotates
slowly – 4 times per hour
A clinostat
 makes factors uniform (evenly distributed)
 for control experiments
 contains a cork disc which can be set to
rotate in a vertical or horizontal plane by a
motor
AIM: To show geotropism in a plant shoot.
APPARATUS:
CONTROL
Give a precaution for this experiment.
Even illumination.
The apparatus was
left for 3 days.
Why did the shoot attached to the clinostat
grow horizontally ?
As shoot rotated, gravity acted on all sides.
No unilateral stimulus to respond to.
AIM: To show geotropism in pea radicles.
APPARATUS:
 The apparatus was left for 3 days.
QUESTION: MAY, 2005
A biology student wanted to test whether the
position in which seeds are placed in the soil
affects the direction of shoot and root growth.
Design a simple experiment which the student
can use to carry out this investigation.(5)
 Seeds of the same type are placed at
different orientations in a single container.
 A similar container is set up as a replicate.
 Left in darkness for a few days.
 Radicles are observed.
QUESTION: MAY, 2005
When growing plants from cuttings, farmers
usually dip the cutting into Rooting Hormone
powder before planting it in the soil. Briefly
explain how this increases the chances of
successful plant growth. (2)
Roots are stimulated to form.
Thus plant can absorb more
water and ions for growth due
to more root hairs present.
AIM: To show phototropism in oat coleoptiles.
APPARATUS:
METHOD:
1. Thirty oat grains were soaked in water for 1 day.
2. Ten grains were placed in each of three small pots of
compost and left to germinate.
3. When grown to a height of 1cm, they were treated as
follows:
 One set was covered with a large box, excluding
light;
 Another set was covered with a box with a slit at
the side, allowing one-sided illumination;
 The control was left in the light.
4. The pots were left at room temperature for a few
days.
RESULT:
 In the dark: coleoptiles were yellow, straight and
very long.
 In one-sided illumination: coleoptiles bent towards
the light.
 In the light: coleoptiles were green, straight and
shorter than those in the dark.
CONCLUSION:
 The coleoptiles responded to the stimulus of one-
sided illumination by growing towards it. Coleoptiles
are positively phototropic.
 Light is needed for chlorophyll to form.
AIM: To show phototropism in a shoot.
APPARATUS:
unilateral
light
unilateral
light
light-proof
box
clinostat
A B
unilateral
light
unilateral
light
light-proof
box
clinostat
The shoots in pot A respond by growing towards the
light source. The shoot in pot B do not show any
curvature but grow vertically upwards...
What has happened to the shoots of plants A and B ?
A B
unilateral
light
unilateral
light
light-proof
box
clinostat
A B
What is the effect of the clinostat on the shoot of plant B ?
The revolving clinostat cancel out the effect of
unilateral light on the shoot of plant B.
VIEW THE ACTUAL EXPERIMENT
IN THE NEXT
3 SLIDES
Question: MAY, 2006
5. The experiment shows the effect of a stimulus
on the growth of a root and a shoot. The seedlings
were kept in the dark throughout the experiment.
a) Continue the diagram to show the direction of growth
of the shoot and root in:
i) the stationary
ii) rotating clinostat. (4)
b) What type of stimulus response is exhibited by
the seedlings? (1)
Geotropism.
c) Why were the seedlings kept in the dark? (2)
Any changes observed would be due to gravity.
d) Name the substance produced by the plant which
brings about the growth changes. (1)
Auxin
e) This experiment was investigated in an orbiting
spacecraft where weightlessness is observed.
Predict the results obtained in this situation. (1)
Grow in all directions.
Under which condition (light/dark)
was each seedling of bean grown?
Grown in the
light
Grown in the
dark
What is the advantage of a
longer stem in seedlings
grown in the dark?
light
dark
Four differences between a seedling
growing in the light & another in the dark.
In the light In the dark
1. Short, thick stem long, thin stem
2. Green shoot yellow shoot
Four differences between a seedling
growing in the light & another in the dark.
In the light In the dark
3. Broad leaves small leaves
4. Short internode long internode
QUESTION: MAY, 2009
Give a biological explanation for each of the
following statements:
the internodes of a shoot growing in the dark
are longer than the internodes of a shoot
growing in the light; (3)
Differences in the response shown by:
A shoot in unilateral light A hand touching a hot
object
Differences in the response shown by:
A shoot in unilateral light A hand touching a hot
object
1. Response is positive. 1. Response is negative
2. Slow response. 2. Rapid response
3. Response involves
growth.
3. No growth involved
4. Response does not
involve muscles and
nerves.
4. Response involves
muscles and nerves.
EXPERIMENTS USING OAT
COLEOPTILES
A Coleoptile is a :
 hollow, cylindrical sheath that surrounds the
primary leaf of a germinating monocot seed
Coleoptile First leaf
no growth grows
dark box
few days later
Conclusion :
The tip is responsible for growth
cut & replaced tip
decapitated
Experiments
on Oat Coleoptiles
unilateral
light
few days later
black covers
Conclusion :
the tip is the place responsible for bending
Experiments
on Oat Coleoptiles
Experiments
on Oat Coleoptiles
few days later
dark box
agar plate
Conclusion :
the head must have produced a diffusible
chemical substance which stimulates growth
Experiments
on Oat Coleoptiles
bending directions
Explanation of results :
The side with the tip has a higher concentration of that particular
chemical, the growth rate is faster than the other side
the tip is put aside
mica
plate
Can the explanation still work ?
bending directions
Tropisms

Tropisms

  • 2.
    TROPISMS  the movementis always a growth movement are responses by part of a plant towards or away from a stimulus coming from one direction
  • 3.
    Tropic responses canbe: Negative – if growth is away from the stimulus Positive – if growth is towards the stimulus Gravity Growth Growth
  • 4.
    Phototropism is a growth responseto light Light Geotropism is a growth response to gravity
  • 5.
  • 6.
    Advantages of ashoot growing:  Towards light:  To trap more light for photosynthesis  Away from gravity:  shoot of a germinating seed grows out of the soil  more chances for pollination & seed dispersal
  • 7.
    A root is: POSITIVELY GEOTROPIC  NEGATIVELY PHOTOTROPIC
  • 8.
    I am growing towardsgravity, but where is the water????
  • 9.
    Advantages of aroot growing towards gravity:  To anchor the plant  To search for water  To ensure that the root of a germinating seed always grows downwards, whatever, the position of the seed in the soil
  • 10.
    Study the picturesbelow and then complete the table by putting a plus (+) if the shoot or root grows towards the stimulus and a minus (-) if it grows away from it. Stimulus Light Gravity Shoot Root
  • 11.
    Study the picturesbelow and then complete the table by putting a plus (+) if the shoot or root grows towards the stimulus and a minus (-) if it grows away from it. Stimulus Light Gravity Shoot + - Root - +
  • 12.
  • 13.
    Study the diagrams.What would you expect to happen and why. The shoot with the covered tip grows (straight up / towards light) because it (can sense light / cannot sense light).
  • 14.
    Study the diagrams.What would you expect to happen and why. The shoot with the covered tip grows (straight up / towards light) because it (can sense light / cannot sense light).
  • 15.
    The shoot withthe exposed tip grows (straight up / towards light) because it (can sense light / cannot sense light).
  • 16.
    The shoot withthe exposed tip grows (straight up / towards light) because it (can sense light / cannot sense light).
  • 17.
    Look at thediagrams below. What does this experiment tell you about the tip of the shoot and the part it plays in growth? Tip produces chemicals for growth.
  • 18.
    Look at thediagrams below. How would you explain the results of this experiment? Bending resulted due to unequal amount of chemical . Light affects distribution of chemical.
  • 19.
  • 20.
    Auxins • are planthormones, or growth regulating chemicals produced in the root and shoot tips that affect growth What is the effect of auxins on plant cells? Cells absorb water and elongate.
  • 21.
    Three regions ofa root tip Cell division region Cell differentiation region Cell elongation region [Cells become specialised]
  • 22.
    Which region doesauxin affect? Cell division region (root tip that makes auxin) Cell differentiation region (cells change their shape; no effect by auxin) Cell elongation region (auxin makes cells absorb water)
  • 23.
    Explain why: markings have movedaway from each other ONLY in the lower region. Cell elongation region
  • 24.
    A shoot growingin even illumination grows straight up. Why? auxin Cells on both sides of the stem elongate equally.
  • 25.
    A shoot growingin one-sided illumination.
  • 26.
    A shoot growingin one-sided illumination grows towards light. How is it possible? Cells on the two sides of the stem GROW UNEQUALLY!!
  • 27.
    Remember: UNEQUAL growthof stem sides results in BENDING of stem Lighted side: normal size of cells Shaded side of stem: cells elongate Light
  • 28.
    Describe how ashoot bends towards one-sided illumination. Auxins are produced at the ___________ tip and diffuse down the stem. More auxin collects on the ____________ side of the stem. Auxins cause the cells to absorb water and so elongate in the cell elongation region. Thus the ___________ side grows more than the lighted one. This results in bending of the stem towards light. shoot shaded shaded
  • 29.
    Auxins sometimes stopgrowth Auxin passes down the stem So the plant grows tall and straight. 1. Apical bud produces auxin. 2. Lateral buds do not grow into side branches. 3.
  • 30.
    If the topis cut off from the plant:  flow of auxin stops  side branches will grow out
  • 31.
    Sometimes gardeners cutthe tops off plants to make them more bushy
  • 32.
    QUESTION: MAY, 2009 Givea biological explanation for each of the following statements: cutting off the tip of a shoot will stop the shoot from getting taller but it will increase bushiness; (3) Cutting off the tip of a shoot removes the source of auxin. Lack of auxin stimulates lateral buds to develop and side branches grow.
  • 33.
    EXPERIMENTS TO SHOW: 1.Geotropism in a shoot 2. Geotropism in pea radicles 3. Phototropism in oat coleoptiles 4. Phototropism in a shoot All make use of an apparatus called: clinostat A motor that rotates slowly – 4 times per hour
  • 34.
    A clinostat  makesfactors uniform (evenly distributed)  for control experiments  contains a cork disc which can be set to rotate in a vertical or horizontal plane by a motor
  • 35.
    AIM: To showgeotropism in a plant shoot. APPARATUS: CONTROL Give a precaution for this experiment. Even illumination. The apparatus was left for 3 days.
  • 36.
    Why did theshoot attached to the clinostat grow horizontally ? As shoot rotated, gravity acted on all sides. No unilateral stimulus to respond to.
  • 37.
    AIM: To showgeotropism in pea radicles. APPARATUS:  The apparatus was left for 3 days.
  • 38.
    QUESTION: MAY, 2005 Abiology student wanted to test whether the position in which seeds are placed in the soil affects the direction of shoot and root growth. Design a simple experiment which the student can use to carry out this investigation.(5)
  • 39.
     Seeds ofthe same type are placed at different orientations in a single container.  A similar container is set up as a replicate.  Left in darkness for a few days.  Radicles are observed.
  • 40.
    QUESTION: MAY, 2005 Whengrowing plants from cuttings, farmers usually dip the cutting into Rooting Hormone powder before planting it in the soil. Briefly explain how this increases the chances of successful plant growth. (2) Roots are stimulated to form. Thus plant can absorb more water and ions for growth due to more root hairs present.
  • 41.
    AIM: To showphototropism in oat coleoptiles. APPARATUS:
  • 42.
    METHOD: 1. Thirty oatgrains were soaked in water for 1 day. 2. Ten grains were placed in each of three small pots of compost and left to germinate. 3. When grown to a height of 1cm, they were treated as follows:  One set was covered with a large box, excluding light;  Another set was covered with a box with a slit at the side, allowing one-sided illumination;  The control was left in the light. 4. The pots were left at room temperature for a few days.
  • 43.
    RESULT:  In thedark: coleoptiles were yellow, straight and very long.  In one-sided illumination: coleoptiles bent towards the light.  In the light: coleoptiles were green, straight and shorter than those in the dark. CONCLUSION:  The coleoptiles responded to the stimulus of one- sided illumination by growing towards it. Coleoptiles are positively phototropic.  Light is needed for chlorophyll to form.
  • 44.
    AIM: To showphototropism in a shoot. APPARATUS: unilateral light unilateral light light-proof box clinostat A B
  • 45.
    unilateral light unilateral light light-proof box clinostat The shoots inpot A respond by growing towards the light source. The shoot in pot B do not show any curvature but grow vertically upwards... What has happened to the shoots of plants A and B ? A B
  • 46.
    unilateral light unilateral light light-proof box clinostat A B What isthe effect of the clinostat on the shoot of plant B ? The revolving clinostat cancel out the effect of unilateral light on the shoot of plant B.
  • 47.
    VIEW THE ACTUALEXPERIMENT IN THE NEXT 3 SLIDES
  • 51.
    Question: MAY, 2006 5.The experiment shows the effect of a stimulus on the growth of a root and a shoot. The seedlings were kept in the dark throughout the experiment.
  • 52.
    a) Continue thediagram to show the direction of growth of the shoot and root in: i) the stationary ii) rotating clinostat. (4)
  • 53.
    b) What typeof stimulus response is exhibited by the seedlings? (1) Geotropism. c) Why were the seedlings kept in the dark? (2) Any changes observed would be due to gravity. d) Name the substance produced by the plant which brings about the growth changes. (1) Auxin
  • 54.
    e) This experimentwas investigated in an orbiting spacecraft where weightlessness is observed. Predict the results obtained in this situation. (1) Grow in all directions.
  • 55.
    Under which condition(light/dark) was each seedling of bean grown? Grown in the light Grown in the dark
  • 56.
    What is theadvantage of a longer stem in seedlings grown in the dark? light dark
  • 57.
    Four differences betweena seedling growing in the light & another in the dark. In the light In the dark 1. Short, thick stem long, thin stem 2. Green shoot yellow shoot
  • 58.
    Four differences betweena seedling growing in the light & another in the dark. In the light In the dark 3. Broad leaves small leaves 4. Short internode long internode
  • 59.
    QUESTION: MAY, 2009 Givea biological explanation for each of the following statements: the internodes of a shoot growing in the dark are longer than the internodes of a shoot growing in the light; (3)
  • 60.
    Differences in theresponse shown by: A shoot in unilateral light A hand touching a hot object
  • 61.
    Differences in theresponse shown by: A shoot in unilateral light A hand touching a hot object 1. Response is positive. 1. Response is negative 2. Slow response. 2. Rapid response 3. Response involves growth. 3. No growth involved 4. Response does not involve muscles and nerves. 4. Response involves muscles and nerves.
  • 62.
  • 63.
    A Coleoptile isa :  hollow, cylindrical sheath that surrounds the primary leaf of a germinating monocot seed Coleoptile First leaf
  • 64.
    no growth grows darkbox few days later Conclusion : The tip is responsible for growth cut & replaced tip decapitated Experiments on Oat Coleoptiles
  • 65.
    unilateral light few days later blackcovers Conclusion : the tip is the place responsible for bending Experiments on Oat Coleoptiles
  • 66.
    Experiments on Oat Coleoptiles fewdays later dark box agar plate Conclusion : the head must have produced a diffusible chemical substance which stimulates growth
  • 67.
    Experiments on Oat Coleoptiles bendingdirections Explanation of results : The side with the tip has a higher concentration of that particular chemical, the growth rate is faster than the other side the tip is put aside mica plate Can the explanation still work ? bending directions