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Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
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Normal Cell Metabolism:
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IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
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Seroprevalence and associated factors of trichinellosis in indigenous pigs and rural communities in Northern Vietnam
1. Seroprevalence and associated factors of trichinellosis in indigenous pigs and
rural communities in Northern Vietnam
Sinh Dang-Xuan1, Nga Vu-Thi2, Dung Do-Trung3, Trang Le-Thi-Huyen1, Ngoc Pham-Thi2, Fred Unger1
1International Livestock Research Institute, Hanoi, Vietnam; 2National Institute of Veterinary Research, Hanoi, Vietnam; 3National Institute of Malaria, Parasitology, and
Entomology, Hanoi, Vietnam
Parasitic pig-borne diseases (PPBD) such as cysticercosis and
trichinellosis continues a to be public health burden to LMIC. Both
are neglected diseases and expected to circulate to an unknown
extend among minority communities and mountainous areas in SEA
including Vietnam. Current information on trichinellosis PPBD is
scarce. A better understanding of the current situation for
trichinellosis is important to assist risk communication and
management to further reduce the zoonotic burden of this diseases
for the community.
A cross-sectional study was conducted in 2021 in Vietnam in ten communes
in Bac Yen district (Son La province) and Bat Xat district (Lao Cai provinces
1. INTRODUCTION
2. METHODOLOGY
500 indigenous pigs' serum
Study sites
3. RESULTS
Fig 3. Seroprevalence of trichinellosis in indigenous pigs and rural
communities in 10 communes in studied provinces
4. CONCLUSIONS
The result shows that seroprevalence of trichinellosis in indigenous pigs and
humans were 9.0% (45/500, 95% CI: 6.7-11.9) and 13.4% (67/500, 95%CI:
10.6-16.8), respectively. Trichinella seroprevalence in human was significantly
higher in Son La (16.7%) compared to in Lao Cai (8.5%), but not for
seroprevalence in pigs.
Seroprevalence in both pigs and humans in northern Vietnam were
relatively high, especially in the endemic province, which suggested the
possibility of Trichinella spp. circulation in the community and
indigenous pigs. Animal and public health awareness for the northern
communities and pig farmers, i.e., using One health approach, are
necessary to improve prevention and control this disease.
Fig 2. Seroprevalence of trichinellosis in indigenous pigs and rural
communities in two provinces (%)
Contact: Sinh Dang-Xuan (S.Dang@cgiar.org); Fred Unger (F.unger@cgiar.org)
Fig 1. Map of study sites in Vietnam and two selected provinces (Son La and Lao
Cai (A), two selected districts in Son La and Lao Cai provinces (B and C), and five selected communes
in those two districts (D and E).
500 community participants
ELISA test kit (Priocheck,
ThermoFisher) for serology
test
Structure questionnaires were
also used to interview selected
pig owners and sampled
community members regarding
pig raising practice, eating
behavior and disease knowledge
and prevention.
Factors associated with trichinellosis seroprevalence in human were
raising pigs’ activities (p=0.029, Fisher test) and eating raw vegetable
behavior (p=0.007, Chi-square test). While factors associated with
trichinellosis seroprevalence in pigs were farmer’s knowledge about
trichinellosis (p=0.033, Chi-squared test)
This publication is copyrighted by the International Livestock Research Institute (ILRI). It is licensed for use under the Creative
Commons Attribution 4.0 International License. August 2022