This document discusses transcription factors and summarizes key details about TBP and p53. It describes transcription factors as DNA-binding proteins that recognize specific DNA sequences and form control modules that regulate gene expression. It then provides structural details of TBP, including how its saddle-shaped structure binds to the minor groove of DNA and induces bending, unwinding and widening. The document also summarizes key functions and regulatory mechanisms of the tumor suppressor protein p53, including its DNA-binding domain and oligomerization domain.
Unit II -examples of protein sec.structure.pptxAravindS199
1. The document discusses the structure-function correlation in various proteins including transcription factors like TATA box binding proteins, tumor suppressor protein p53, and signal transducer proteins.
2. It describes the DNA binding domains of transcription factors like TATA box binding protein which binds to the minor groove of DNA, and p53 which uses loops and alpha helices to interact with DNA in both the major and minor grooves.
3. Zinc finger proteins and leucine zipper domains are discussed as examples of DNA binding motifs, with zinc fingers using cysteine and histidine residues to coordinate zinc ions and form DNA interacting loops and helices, while leucine zippers enable dimerization of transcription factors via
TATA binding proteins (TBPs) play an essential role in eukaryotic transcription. TBP is a subunit of the general transcription factor TFIID that binds to the TATA box upstream of core promoters. TBP binds in the minor groove of DNA and bends it into an 80 degree curve. TBP adopts a saddle-shaped structure that positions the concave surface to interact with DNA while exposing the convex surface to recruit other general transcription factors and form the preinitiation complex. TBP is universally required for transcription by all three eukaryotic RNA polymerases and some genes in Archaea as well.
Transcription is the process of synthesizing RNA using a DNA template. There are four main types of RNA - mRNA, tRNA, rRNA and snRNA. Transcription involves initiation, elongation and termination. In initiation, RNA polymerase binds to a promoter and transcription begins. In elongation, RNA is continuously synthesized using the DNA as a template. Termination occurs when RNA polymerase stops moving along the DNA template. Eukaryotic transcription requires transcription factors to help RNA polymerase bind DNA, while prokaryotic transcription involves direct binding of RNA polymerase to DNA. The nascent RNA transcript undergoes processing including capping, polyadenylation, splicing and editing to become a mature RNA.
The document discusses the basic principles of gene expression from DNA to protein. It describes transcription, which is the synthesis of RNA from a DNA template, and translation, which is the synthesis of proteins from mRNA templates using ribosomes. In eukaryotes, transcription requires RNA polymerases and other transcription factors to initiate transcription from DNA. The primary transcript then undergoes processing including 5' capping, 3' polyadenylation, and splicing to form mature mRNA. The mRNA is then translated by ribosomes to produce proteins.
The document discusses the structure and function of DNA and RNA. It describes DNA as a double-stranded helical structure composed of deoxyribonucleotides held together by phosphodiester bonds. The bases adenine, guanine, cytosine and thymine form hydrogen bonds between the strands in a complementary fashion according to Watson-Crick base pairing rules. RNA is single-stranded and exists in various types that serve different functions, such as messenger RNA, transfer RNA and ribosomal RNA, which are involved in protein synthesis. The structures of DNA and RNA allow them to carry out their roles in genetic inheritance and expression.
Cells contain three main types of RNA: rRNA, tRNA, and mRNA. rRNA makes up ribosomes, tRNA transports amino acids during protein synthesis, and mRNA directs protein synthesis. Transcription is carried out by RNA polymerases that resemble DNA polymerases. In prokaryotes, transcription initiates at promoter sequences and terminates at specific termination sites. Eukaryotes have multiple RNA polymerases and more complex transcription initiation involving general transcription factors that help recruit the polymerase and direct proper initiation.
RNA polymerases are enzymes that transcribe DNA into RNA. In prokaryotes, a single RNA polymerase synthesizes RNA, while eukaryotes contain three RNA polymerases that synthesize different RNA molecules. RNA polymerases are large complex protein machines made of multiple subunits that work together to unwind DNA, add nucleotides, and proofread the newly synthesized RNA. The transcription process involves initiation, elongation, and termination stages that are regulated by various transcription factors.
The flow of information in the cell starts at DNA, which replicates to form more DNA. Information is then ‘transcribed” into RNA, and then it is “translated” into protein.
Information does not flow in the other direction.
A few exceptions to the Central Dogma exist
some RNA viruses, called “retroviruses”.
Unit II -examples of protein sec.structure.pptxAravindS199
1. The document discusses the structure-function correlation in various proteins including transcription factors like TATA box binding proteins, tumor suppressor protein p53, and signal transducer proteins.
2. It describes the DNA binding domains of transcription factors like TATA box binding protein which binds to the minor groove of DNA, and p53 which uses loops and alpha helices to interact with DNA in both the major and minor grooves.
3. Zinc finger proteins and leucine zipper domains are discussed as examples of DNA binding motifs, with zinc fingers using cysteine and histidine residues to coordinate zinc ions and form DNA interacting loops and helices, while leucine zippers enable dimerization of transcription factors via
TATA binding proteins (TBPs) play an essential role in eukaryotic transcription. TBP is a subunit of the general transcription factor TFIID that binds to the TATA box upstream of core promoters. TBP binds in the minor groove of DNA and bends it into an 80 degree curve. TBP adopts a saddle-shaped structure that positions the concave surface to interact with DNA while exposing the convex surface to recruit other general transcription factors and form the preinitiation complex. TBP is universally required for transcription by all three eukaryotic RNA polymerases and some genes in Archaea as well.
Transcription is the process of synthesizing RNA using a DNA template. There are four main types of RNA - mRNA, tRNA, rRNA and snRNA. Transcription involves initiation, elongation and termination. In initiation, RNA polymerase binds to a promoter and transcription begins. In elongation, RNA is continuously synthesized using the DNA as a template. Termination occurs when RNA polymerase stops moving along the DNA template. Eukaryotic transcription requires transcription factors to help RNA polymerase bind DNA, while prokaryotic transcription involves direct binding of RNA polymerase to DNA. The nascent RNA transcript undergoes processing including capping, polyadenylation, splicing and editing to become a mature RNA.
The document discusses the basic principles of gene expression from DNA to protein. It describes transcription, which is the synthesis of RNA from a DNA template, and translation, which is the synthesis of proteins from mRNA templates using ribosomes. In eukaryotes, transcription requires RNA polymerases and other transcription factors to initiate transcription from DNA. The primary transcript then undergoes processing including 5' capping, 3' polyadenylation, and splicing to form mature mRNA. The mRNA is then translated by ribosomes to produce proteins.
The document discusses the structure and function of DNA and RNA. It describes DNA as a double-stranded helical structure composed of deoxyribonucleotides held together by phosphodiester bonds. The bases adenine, guanine, cytosine and thymine form hydrogen bonds between the strands in a complementary fashion according to Watson-Crick base pairing rules. RNA is single-stranded and exists in various types that serve different functions, such as messenger RNA, transfer RNA and ribosomal RNA, which are involved in protein synthesis. The structures of DNA and RNA allow them to carry out their roles in genetic inheritance and expression.
Cells contain three main types of RNA: rRNA, tRNA, and mRNA. rRNA makes up ribosomes, tRNA transports amino acids during protein synthesis, and mRNA directs protein synthesis. Transcription is carried out by RNA polymerases that resemble DNA polymerases. In prokaryotes, transcription initiates at promoter sequences and terminates at specific termination sites. Eukaryotes have multiple RNA polymerases and more complex transcription initiation involving general transcription factors that help recruit the polymerase and direct proper initiation.
RNA polymerases are enzymes that transcribe DNA into RNA. In prokaryotes, a single RNA polymerase synthesizes RNA, while eukaryotes contain three RNA polymerases that synthesize different RNA molecules. RNA polymerases are large complex protein machines made of multiple subunits that work together to unwind DNA, add nucleotides, and proofread the newly synthesized RNA. The transcription process involves initiation, elongation, and termination stages that are regulated by various transcription factors.
The flow of information in the cell starts at DNA, which replicates to form more DNA. Information is then ‘transcribed” into RNA, and then it is “translated” into protein.
Information does not flow in the other direction.
A few exceptions to the Central Dogma exist
some RNA viruses, called “retroviruses”.
This document discusses the process of transcription from DNA to RNA. It begins with an overview of transcription in prokaryotes and eukaryotes. In prokaryotes, RNA polymerase binds to promoter sequences and transcribes DNA into RNA. Eukaryotes have three RNA polymerases that recognize different promoters. The document then covers the stages of transcription including initiation, elongation, and termination. It describes the requirements for transcription including the DNA template, RNA polymerase, and ribonucleotide substrates. Differences between DNA and RNA as well as between prokaryotic and eukaryotic transcription are also summarized.
Prokaryotic transcription involves RNA polymerase binding to promoter sequences on DNA and synthesizing RNA without the need for primers. It proceeds through initiation, elongation, and termination stages. Eukaryotic transcription is more complex, utilizing three RNA polymerases and involving transcription factors, mediator complexes, 5' capping, splicing, and 3' polyadenylation to process mRNA. Alternative splicing allows single genes to code for multiple proteins through different combinations of exons.
Transcription is the process by which RNA is synthesized from a DNA template. It involves three main steps - initiation, elongation, and termination. In prokaryotes, RNA polymerase binds directly to the promoter region of DNA and initiates transcription. Eukaryotes require various transcription factors to help RNA polymerase bind to the promoter. The transcription process is similar between prokaryotes and eukaryotes, but eukaryotes have three types of RNA polymerase and more complex regulation. Reverse transcription is the process by which DNA is synthesized from an RNA template using the enzyme reverse transcriptase.
This document summarizes the key processes of transcription in prokaryotes and eukaryotes. It describes how transcription involves initiation, elongation, and termination phases. In prokaryotes, RNA polymerase directly binds DNA and synthesizes RNA from 5' to 3'. In eukaryotes, RNA polymerase requires transcription factors to initiate transcription. The document also discusses post-transcriptional modifications of pre-mRNA in eukaryotes including 5' capping, 3' polyadenylation, splicing of exons and introns, and RNA editing.
description of mechanism of transcription in prokaryotes and eukaryotes with clear explanation and clear pictures and also mentiong of different promotors and enhancers and silencers
The document summarizes transcription in prokaryotes. It discusses the key components including the template strand, coding strand, and RNA polymerase. RNA polymerase is made up of multiple subunits and recognizes promoter sequences to initiate transcription. The process of transcription involves three phases - initiation when RNA polymerase binds to the promoter, elongation as the RNA strand continuously grows, and termination when RNA polymerase stops synthesis.
The central dogma of molecular biology describes the flow of genetic information within living systems:
1. DNA is transcribed into RNA which is then translated into protein.
2. RNA acts as an intermediary between DNA and protein, carrying copies of instructions from DNA to direct protein synthesis.
3. The genetic code allows information stored in nucleic acids like DNA and RNA to be "translated" into proteins through triplet codons that specify the 20 standard amino acids.
DNA contains the genetic instructions for all living organisms. It is made up of nucleotides with a sugar-phosphate backbone that form a double helix structure. The nucleotides contain nitrogenous bases, which pair up through hydrogen bonding between adenine and thymine and between cytosine and guanine. This precise base pairing allows DNA to replicate and transmit genetic information from parent to daughter cells.
PCR is a technique used to amplify a specific region of DNA across multiple cycles. It involves separating the DNA strands through heating, followed by primers annealing to the complementary DNA sequences. The DNA polymerase then extends the strands to exponentially increase copies of the target DNA. PCR has many applications in molecular biology, forensics, disease diagnosis and more due to its ability to amplify very small amounts of DNA.
DNA replication is the process where a cell makes an identical copy of its DNA before cell division. It involves unwinding the DNA double helix into single strands, and using DNA polymerase to add complementary nucleotides to each strand to make two new double helix DNA molecules. It is semiconservative, starting at the origin of replication and proceeding bidirectionally. The leading strand is synthesized continuously while the lagging strand makes short Okazaki fragments that are later joined. DNA replication occurs with high fidelity to maintain genetic integrity as cells divide.
1. DNA contains the genetic code and is replicated and transcribed into mRNA, which is then translated into protein. During replication, DNA polymerase adds nucleotides to the growing DNA strand while helicase unwinds the double helix.
2. Transcription involves RNA polymerase binding to DNA and synthesizing mRNA, which then undergoes processing. Translation uses tRNA to decode the mRNA codon by codon, adding the corresponding amino acids specified by the genetic code to form a polypeptide chain.
3. Both transcription and translation are complex processes involving many proteins and enzymes to proofread and maintain fidelity. DNA, RNA and proteins are synthesized through the coordinated actions of replication, transcription and translation.
Transcription in prokaryotes involves RNA polymerase binding to specific promoter sequences on DNA and synthesizing RNA. It occurs in three main steps - initiation at the promoter, elongation as the RNA chain grows, and termination. Key elements that regulate transcription include the -10, -35 promoter sequences, sigma factor, and rho-dependent or intrinsic terminator sequences.
Prokaryotes are organisms that consist of a single prokaryotic cell. Eukaryotic cells are found in plants, animals, fungi, and protists. They range from 10–100 μm in diameter, and their DNA is contained within a membrane-bound nucleus.Prokaryotes do not have membrane-enclosed nuclei. Therefore, the processes of transcription, translation, and mRNA degradation can all occur simultaneously.
Transcriptional and post transcriptional regulation of gene expressionDr. Kirti Mehta
Gene expression is regulated at the transcriptional and post-transcriptional levels. Transcriptional regulation involves proteins binding to promoter and enhancer sequences to control RNA polymerase recruitment and initiation of transcription. Eukaryotic gene expression requires transcription factors, coactivators, and basal transcription factors to assemble the transcription initiation complex. Post-transcriptional regulation influences RNA processing, transport, translation, and degradation.
Transcription is the process of copying DNA into RNA. In bacteria, RNA polymerase binds to promoter sequences and initiates transcription. It then elongates the RNA transcript by adding nucleotides complementary to the DNA template. Termination occurs via either Rho-dependent or Rho-independent mechanisms when the polymerase reaches a terminator sequence. In eukaryotes, RNA polymerase and transcription factors bind to promoter elements like the TATA box to initiate transcription. Termination is triggered by a polyadenylation signal that causes cleavage of the transcript.
Dr. Sangeeta Khyalia discusses the central dogma of biology and the processes of transcription and translation. Transcription is the synthesis of RNA using DNA as a template, catalyzed by the enzyme RNA polymerase. It involves initiation, elongation, and termination. Eukaryotic transcription differs from prokaryotic transcription in initiation and processing of pre-mRNA. The document provides details on RNA polymerases, promoters, inhibitors of transcription, and post-transcriptional modifications of pre-mRNA in eukaryotes like splicing and polyadenylation.
This document provides an overview of molecular biology concepts including:
- The central dogma of DNA -> RNA -> Protein
- Differences between prokaryotic and eukaryotic cells
- Structure and packaging of DNA and RNA
- DNA replication, transcription, and translation
- Genetic code and how triplet codons specify amino acids
- Role of tRNAs and ribosomes in protein synthesis
PCR is a technique used to amplify specific DNA sequences. It involves repeated cycles of separating DNA strands through heating, annealing primers to the strands, and extending the strands with DNA polymerase. This process can produce billions of copies of the target DNA fragment. PCR is used in research, forensics, and medicine to detect genetic mutations and diseases.
This document summarizes transcriptional gene regulation in eukaryotes. It discusses the basic mechanisms of transcription including initiation, elongation, and termination. It describes the role of general transcription factors, promoter elements like TATA boxes, and RNA polymerase in basal transcription. It also explains how distal enhancer elements and transcription factors regulate gene expression by interacting with the promoter and basal transcription machinery. Chromatin structure is also noted to influence transcription. The modular and combinatorial nature of transcriptional control is emphasized.
This document discusses the process of transcription from DNA to RNA. It begins with an overview of transcription in prokaryotes and eukaryotes. In prokaryotes, RNA polymerase binds to promoter sequences and transcribes DNA into RNA. Eukaryotes have three RNA polymerases that recognize different promoters. The document then covers the stages of transcription including initiation, elongation, and termination. It describes the requirements for transcription including the DNA template, RNA polymerase, and ribonucleotide substrates. Differences between DNA and RNA as well as between prokaryotic and eukaryotic transcription are also summarized.
Prokaryotic transcription involves RNA polymerase binding to promoter sequences on DNA and synthesizing RNA without the need for primers. It proceeds through initiation, elongation, and termination stages. Eukaryotic transcription is more complex, utilizing three RNA polymerases and involving transcription factors, mediator complexes, 5' capping, splicing, and 3' polyadenylation to process mRNA. Alternative splicing allows single genes to code for multiple proteins through different combinations of exons.
Transcription is the process by which RNA is synthesized from a DNA template. It involves three main steps - initiation, elongation, and termination. In prokaryotes, RNA polymerase binds directly to the promoter region of DNA and initiates transcription. Eukaryotes require various transcription factors to help RNA polymerase bind to the promoter. The transcription process is similar between prokaryotes and eukaryotes, but eukaryotes have three types of RNA polymerase and more complex regulation. Reverse transcription is the process by which DNA is synthesized from an RNA template using the enzyme reverse transcriptase.
This document summarizes the key processes of transcription in prokaryotes and eukaryotes. It describes how transcription involves initiation, elongation, and termination phases. In prokaryotes, RNA polymerase directly binds DNA and synthesizes RNA from 5' to 3'. In eukaryotes, RNA polymerase requires transcription factors to initiate transcription. The document also discusses post-transcriptional modifications of pre-mRNA in eukaryotes including 5' capping, 3' polyadenylation, splicing of exons and introns, and RNA editing.
description of mechanism of transcription in prokaryotes and eukaryotes with clear explanation and clear pictures and also mentiong of different promotors and enhancers and silencers
The document summarizes transcription in prokaryotes. It discusses the key components including the template strand, coding strand, and RNA polymerase. RNA polymerase is made up of multiple subunits and recognizes promoter sequences to initiate transcription. The process of transcription involves three phases - initiation when RNA polymerase binds to the promoter, elongation as the RNA strand continuously grows, and termination when RNA polymerase stops synthesis.
The central dogma of molecular biology describes the flow of genetic information within living systems:
1. DNA is transcribed into RNA which is then translated into protein.
2. RNA acts as an intermediary between DNA and protein, carrying copies of instructions from DNA to direct protein synthesis.
3. The genetic code allows information stored in nucleic acids like DNA and RNA to be "translated" into proteins through triplet codons that specify the 20 standard amino acids.
DNA contains the genetic instructions for all living organisms. It is made up of nucleotides with a sugar-phosphate backbone that form a double helix structure. The nucleotides contain nitrogenous bases, which pair up through hydrogen bonding between adenine and thymine and between cytosine and guanine. This precise base pairing allows DNA to replicate and transmit genetic information from parent to daughter cells.
PCR is a technique used to amplify a specific region of DNA across multiple cycles. It involves separating the DNA strands through heating, followed by primers annealing to the complementary DNA sequences. The DNA polymerase then extends the strands to exponentially increase copies of the target DNA. PCR has many applications in molecular biology, forensics, disease diagnosis and more due to its ability to amplify very small amounts of DNA.
DNA replication is the process where a cell makes an identical copy of its DNA before cell division. It involves unwinding the DNA double helix into single strands, and using DNA polymerase to add complementary nucleotides to each strand to make two new double helix DNA molecules. It is semiconservative, starting at the origin of replication and proceeding bidirectionally. The leading strand is synthesized continuously while the lagging strand makes short Okazaki fragments that are later joined. DNA replication occurs with high fidelity to maintain genetic integrity as cells divide.
1. DNA contains the genetic code and is replicated and transcribed into mRNA, which is then translated into protein. During replication, DNA polymerase adds nucleotides to the growing DNA strand while helicase unwinds the double helix.
2. Transcription involves RNA polymerase binding to DNA and synthesizing mRNA, which then undergoes processing. Translation uses tRNA to decode the mRNA codon by codon, adding the corresponding amino acids specified by the genetic code to form a polypeptide chain.
3. Both transcription and translation are complex processes involving many proteins and enzymes to proofread and maintain fidelity. DNA, RNA and proteins are synthesized through the coordinated actions of replication, transcription and translation.
Transcription in prokaryotes involves RNA polymerase binding to specific promoter sequences on DNA and synthesizing RNA. It occurs in three main steps - initiation at the promoter, elongation as the RNA chain grows, and termination. Key elements that regulate transcription include the -10, -35 promoter sequences, sigma factor, and rho-dependent or intrinsic terminator sequences.
Prokaryotes are organisms that consist of a single prokaryotic cell. Eukaryotic cells are found in plants, animals, fungi, and protists. They range from 10–100 μm in diameter, and their DNA is contained within a membrane-bound nucleus.Prokaryotes do not have membrane-enclosed nuclei. Therefore, the processes of transcription, translation, and mRNA degradation can all occur simultaneously.
Transcriptional and post transcriptional regulation of gene expressionDr. Kirti Mehta
Gene expression is regulated at the transcriptional and post-transcriptional levels. Transcriptional regulation involves proteins binding to promoter and enhancer sequences to control RNA polymerase recruitment and initiation of transcription. Eukaryotic gene expression requires transcription factors, coactivators, and basal transcription factors to assemble the transcription initiation complex. Post-transcriptional regulation influences RNA processing, transport, translation, and degradation.
Transcription is the process of copying DNA into RNA. In bacteria, RNA polymerase binds to promoter sequences and initiates transcription. It then elongates the RNA transcript by adding nucleotides complementary to the DNA template. Termination occurs via either Rho-dependent or Rho-independent mechanisms when the polymerase reaches a terminator sequence. In eukaryotes, RNA polymerase and transcription factors bind to promoter elements like the TATA box to initiate transcription. Termination is triggered by a polyadenylation signal that causes cleavage of the transcript.
Dr. Sangeeta Khyalia discusses the central dogma of biology and the processes of transcription and translation. Transcription is the synthesis of RNA using DNA as a template, catalyzed by the enzyme RNA polymerase. It involves initiation, elongation, and termination. Eukaryotic transcription differs from prokaryotic transcription in initiation and processing of pre-mRNA. The document provides details on RNA polymerases, promoters, inhibitors of transcription, and post-transcriptional modifications of pre-mRNA in eukaryotes like splicing and polyadenylation.
This document provides an overview of molecular biology concepts including:
- The central dogma of DNA -> RNA -> Protein
- Differences between prokaryotic and eukaryotic cells
- Structure and packaging of DNA and RNA
- DNA replication, transcription, and translation
- Genetic code and how triplet codons specify amino acids
- Role of tRNAs and ribosomes in protein synthesis
PCR is a technique used to amplify specific DNA sequences. It involves repeated cycles of separating DNA strands through heating, annealing primers to the strands, and extending the strands with DNA polymerase. This process can produce billions of copies of the target DNA fragment. PCR is used in research, forensics, and medicine to detect genetic mutations and diseases.
This document summarizes transcriptional gene regulation in eukaryotes. It discusses the basic mechanisms of transcription including initiation, elongation, and termination. It describes the role of general transcription factors, promoter elements like TATA boxes, and RNA polymerase in basal transcription. It also explains how distal enhancer elements and transcription factors regulate gene expression by interacting with the promoter and basal transcription machinery. Chromatin structure is also noted to influence transcription. The modular and combinatorial nature of transcriptional control is emphasized.
This presentation was provided by Racquel Jemison, Ph.D., Christina MacLaughlin, Ph.D., and Paulomi Majumder. Ph.D., all of the American Chemical Society, for the second session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session Two: 'Expanding Pathways to Publishing Careers,' was held June 13, 2024.
How to Download & Install Module From the Odoo App Store in Odoo 17Celine George
Custom modules offer the flexibility to extend Odoo's capabilities, address unique requirements, and optimize workflows to align seamlessly with your organization's processes. By leveraging custom modules, businesses can unlock greater efficiency, productivity, and innovation, empowering them to stay competitive in today's dynamic market landscape. In this tutorial, we'll guide you step by step on how to easily download and install modules from the Odoo App Store.
A Free 200-Page eBook ~ Brain and Mind Exercise.pptxOH TEIK BIN
(A Free eBook comprising 3 Sets of Presentation of a selection of Puzzles, Brain Teasers and Thinking Problems to exercise both the mind and the Right and Left Brain. To help keep the mind and brain fit and healthy. Good for both the young and old alike.
Answers are given for all the puzzles and problems.)
With Metta,
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5- الملزمة تشرح نفسها ب نفسها بس تكلك تعال اقراني
6- تحتوي الملزمة في اول سلايد على خارطة تتضمن جميع تفرُعات معلومات الجهاز الهيكلي المذكورة في هذهِ الملزمة
واخيراً هذهِ الملزمة حلالٌ عليكم وإتمنى منكم إن تدعولي بالخير والصحة والعافية فقط
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How to Manage Reception Report in Odoo 17Celine George
A business may deal with both sales and purchases occasionally. They buy things from vendors and then sell them to their customers. Such dealings can be confusing at times. Because multiple clients may inquire about the same product at the same time, after purchasing those products, customers must be assigned to them. Odoo has a tool called Reception Report that can be used to complete this assignment. By enabling this, a reception report comes automatically after confirming a receipt, from which we can assign products to orders.
2. What is transcription factor?
Distal to the RNA Pol II initiation site, there are different combinations of
specific DNA binding sequences each of which is recognized by a
corresponding site specific DNA binding protein.
These proteins are known as transcription factor(s).
These together with DNA form the control module of gene expression
Example: TFIID,TFIIA,TFIIB, TBP etc
3. Architecture of a structural gene and the promoter(control module)
Core promoter element
4. TATA Box:
• A-T Rich 8 base pair DNA sequence
• Located 25 base pair upstream of of TSS
• Recognized by TATA Box binding Proteins (TBPs)
5. Promoter proximal Element:
• 100-200 bp long
• Several transcription factors interact directly or indirectly with the pre
initiation complex
Enhancer Element:
• Resides further upstream or down stream of the TSS
• Few thousand to 20000 bp distant from the TSS
6. Schematic model of transcriptional activation
Transcription factor Bind to the DNA
Transcriptional Activation
8. 1. TATA Box Binding Protein (TBP)
• First isolated and purified from Yeast in 1988
• Single polypeptide chain of 27 kDa
• Conserved C Terminal domain of 180 aa
• N Terminal domain of varied length and diverse sequences
• C terminal domain having DNA binding and transcription
activation function
Structure of TBP
Crystal structure by Paul Sigler @ Yale University With
Yeast C trminal TBP and Yeast TATA box DNA
Stephen Burley @ Rockyfeller University with C Terminal
TBP of A. thaliana and TATA box DNA from Adeno virus
9. • Two homologous repeat of 88 aa form
similar motifs
• Comprises of an antiparallel Beta sheet of
five strands and Two α- helices
• Two motifs are joined together by a short
loop to make a 10 stranded beta sheet
• They look like a saddle (Fig a)
• (fig b)90◦ rotation of the Fig a
10. • Loops that connects beta strand 2 & 3 of
each motif forms the Stirrups of the
saddle
• Underside of the saddle forms the
conclave surface built by the central eight
strand of beta sheet
• Side chain of this site of beta
• Sheet as well as residues of the Stirrups
forms the DNA binding Site.
• The side of the beta sheet that faces away
from the DNA is covered by two alpha
helices
Residues from these two helices and from the short loop that joins the two motif
Interacts with TFIID and with other transcription factors.
11. How TBP binds to the DNA?
Answer: TBP binds to the minor groove of the DNA
and Induces large structural changes
12. • Normal B-DNA structure returns out side the TATA box
• The helical axis of the DNA at each end of the TATA BOX
form an angle of about 100 degree to each other , instead of the
Expected 180 degree if the DNA was not bent.
• First two and the last two bp of TATA box, there are sharp kinks, DNA is
Covered smoothly and partially unwounded.
13. • Two Phenylalanine residues are partially inserted between first two and the last two bases, preventing
stacking of the adjacent bases and allow increase in rise Of the DNA
• The kinks at each end of the DNA and partial unwinding of the DNA produces a wide and shallow minor
groove.
• This exposed wide and shallow minor groove bind intimately to the concave undersurface of the TBP saddle.
DNA Modifications: Distortions:
a. Bending of DNA
b. Widening of the minor groove
c. Unwinding of the DNA
14. • All eight nucleotides of TATA box interacts with TBP and their structure
deviates from the normal B-DNA.
• Saddle would straddle normal B-DNA structure with helical axis of the DNA
perpendicular to a line connecting the two stirrups.
• DNA is sharply bent at TATA box region so that the local helical axis is
almost is almost parallel to the line from stirrups to stirrups.
Protein
Saddle structure
Minor groove of
DNA
15. What is the nature of the interaction?
• Strong hydrophobic interaction between the underside of TBP saddle and the minor
groove of DNA
• Side chains of eight central beta strands interacts with both the phosphate sugar Backbone
and the minor groove of the eight nucleotides of the TATA box.
• Fifteen side chains projecting from the beta strands make hydrophobic contacts With the
sugar and bases of DNA.
• The phosphate groups are hydrogen bonded to arginine and lysine side chains At the edges
of the interaction area.
16. Why specific to TA/AT sequence at 4 and 5 position of
bp?
Only sequence specific H bonds – center of box
Asn 69 – O2 of T4’ and N3 of A5’
Asn 159 – O2 of T5 and N3 of A4
Thr 124 &215– N3 of A both sides
Role of Conserved Val residues.
Val 71 and 122 on one side
Val 161 and 213 on the other side
Side chains of Val residues cause steric interference with NH2 substituent from
G-C or C-G basepair.
Flanking Val residues in combination with 6 H-bonds specify A-T or T-A at
positions 4 and 5 of TATA box
Why Minor groove???
Quasi – palindromicity
Functional implication of DNA bending
TBF – associated factors (TAF)
Significance of N – terminal in TBP
17. Why strong affinity between TBP-TATA Box? Around 100000 fold
more affinity than random DNA.
• Large interacting hydrophobic surface area
• Major distortion in the DNA
• SIX Hydrogen bonds between 4 side chain residues of TBP and 4
hydrogen bond acceptors from bases In the minor groove.
18. 2. p53
Most ambiguous and cited biological molecule.
Encoded by genes known to be Tumor Suppressor Genes (TSG)????
Protein with 53 kDa MW – promotes expression of p21 – a protein inhibiting
CDK’s (Check point) in the cell cycle-
This gives sufficient time to repair or destruction of damaged cells (apoptosis)
Single point mutation – altered function – observed in more than half of the cancer
patients
wild type – sequence specific DNA binding
mutated p53 – no binding and hence no regulation- leads to no expression of
p21 and hence uncontrolled cell cycle.
19. p53 – Oligomerization Domain
Oligomerization domain – tetramer formation of p53
Mutations in C – terminal affects tetramer formation.
The monomer still retains DNA binding function
No complete structure available
available structures- Sloane Kettering institute for cancer- NewYork
21 base pairs sequence bound to p53 DNA binding region (102 – 292)
Oligomerizing domain (325 – 356)
Each unit of p53 has a beta strand –turn- alpha helix
Two units bind together by antiparallel beta sheet- followed by antiparallel helix
formation
This dimer binds with another dimer by hydrophobic interactions of the helix. Beta
sheets do not interact in the tetramer
Tumorogenic mutations
Leu 330 to His- water loving His does not allow dimerization core to be formed
Glycine in turn if mutated to any other residue - also abolishes p53 dimerization
20. P53 – DNA binding Domain
DNA binding domain (anti-parallel beta barrel)
protruding loops from anti – parallel beta barrel
immunoglobulin fold (7/9 strands)
This kind of fold also present at I– MHC binding coreceptor in CD4
NF-kB – REL homology region