Title:
Media culture
Objectives:
1. To apply aseptic technique.
2. To observe the appearance of different bacteria in different media agar.
3. To explain on the physiology of microbes.
Material/apparatus:
Gloves, Bunsen burner, inoculation loop, incubator, MSA agar, EMB agar, MacConkey agar, blood
agar,
E. coli, E. aerogenes, S. epidermidis, and S. aureus.
Procedure:
1. Label the bottom of petri dish with your name, date and type of media culture.
2. Divide each petri dish into 4 quadrants, by marking the bottom of the dish. Label each
section with the name of bacteria to be inoculated.
3. Using aseptic technique, inoculate all plates except blood agar, with the designated bacteria
by making a single line of inoculation of each bacterium in its appropriate section. Ensure
that the petri dishes are close and the inoculation loop flamed between the inoculations of
different bacteria.
4. Using aseptic technique, inoculate blood agar, with the designated bacteria by making a
single line of inoculation of each bacterium in its appropriate section like in step 3. Upon
completion of each single line of inoculation, use the inoculation loop and make three of four
stabs at a 45 angle across the streak.
5. Incubate the petri dishes in inverted position for 24 to 48 hours at 37C.
6. Observe and record your observation
Results:
Questions:
1. Discuss the appearance of media; with the appearance of bacteria grow on the media.
2. Indicate the specific selective and/or differential purpose of MSA agar, EMB agar, and
MacConkey agar.
3. Explain the purpose of blood in blood agar..
Title Media culture Objectives 1 To apply aseptic techniq.pdf
1. Title:
Media culture
Objectives:
1. To apply aseptic technique.
2. To observe the appearance of different bacteria in different media agar.
3. To explain on the physiology of microbes.
Material/apparatus:
Gloves, Bunsen burner, inoculation loop, incubator, MSA agar, EMB agar, MacConkey agar, blood
agar,
E. coli, E. aerogenes, S. epidermidis, and S. aureus.
Procedure:
1. Label the bottom of petri dish with your name, date and type of media culture.
2. Divide each petri dish into 4 quadrants, by marking the bottom of the dish. Label each
section with the name of bacteria to be inoculated.
3. Using aseptic technique, inoculate all plates except blood agar, with the designated bacteria
by making a single line of inoculation of each bacterium in its appropriate section. Ensure
that the petri dishes are close and the inoculation loop flamed between the inoculations of
different bacteria.
4. Using aseptic technique, inoculate blood agar, with the designated bacteria by making a
single line of inoculation of each bacterium in its appropriate section like in step 3. Upon
completion of each single line of inoculation, use the inoculation loop and make three of four
stabs at a 45 angle across the streak.
5. Incubate the petri dishes in inverted position for 24 to 48 hours at 37C.
6. Observe and record your observation
Results:
Questions:
1. Discuss the appearance of media; with the appearance of bacteria grow on the media.
2. Indicate the specific selective and/or differential purpose of MSA agar, EMB agar, and
MacConkey agar.
3. Explain the purpose of blood in blood agar.
