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Presentation on Tissue Culture
To: Ms. Umama Dewan (Ma’am)
.
Group: ENIGMA
• Md. Rahmat Ullah
161-15-6970
• Abir Hasnat
161-15-6789
What is Tissue Culture???...
• Tissue culture is the science of growing
cells, tissues or organs isolated from the
Mother cell or organs, on artificial media in
vitro under controlled conditions.
•
Classification
• Tissue culture may be of two types based on
medium.
• 1. Plant tissue culture
• 2. Animal tissue culture
History
• HABERLANDT is considered to be the father of plant
tissue culture (1902).
• Although animal cell culture was first successfully
undertaken by Ross Harrison in (1907).
Plant Tissue Culture Steps
• Selection of the plant tissue
• Sterilize tissue
• Establishment of culture medium
• Establishment of the explant in a culture
medium
• Multiplication
• Root formation
• The rooted shoots are potted up
Plant Tissue Culture Steps
.
.
Animal Tissue Culture
• Obtain tissue
• Aseptic the tissue
• Establishment of medium
• Establishment of tissue in medium
• Growing the cells
Animal Tissue Culture
• .
Uses of Tissue Culture
• Micropropagation
• plant breeder
• Large-scale growth of plant
• Cross distantly related species
• Genetic modification & analysis
• Making hormones
• Providing DNA for the Human Genome
Project
Thank You Everyone.
Any Question??
•.

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Tissue Culture

  • 1. Presentation on Tissue Culture To: Ms. Umama Dewan (Ma’am) .
  • 2. Group: ENIGMA • Md. Rahmat Ullah 161-15-6970 • Abir Hasnat 161-15-6789
  • 3. What is Tissue Culture???... • Tissue culture is the science of growing cells, tissues or organs isolated from the Mother cell or organs, on artificial media in vitro under controlled conditions. •
  • 4. Classification • Tissue culture may be of two types based on medium. • 1. Plant tissue culture • 2. Animal tissue culture
  • 5. History • HABERLANDT is considered to be the father of plant tissue culture (1902). • Although animal cell culture was first successfully undertaken by Ross Harrison in (1907).
  • 6. Plant Tissue Culture Steps • Selection of the plant tissue • Sterilize tissue • Establishment of culture medium • Establishment of the explant in a culture medium • Multiplication • Root formation • The rooted shoots are potted up
  • 8. Animal Tissue Culture • Obtain tissue • Aseptic the tissue • Establishment of medium • Establishment of tissue in medium • Growing the cells
  • 10. Uses of Tissue Culture • Micropropagation • plant breeder • Large-scale growth of plant • Cross distantly related species • Genetic modification & analysis • Making hormones • Providing DNA for the Human Genome Project
  • 11. Thank You Everyone. Any Question?? •.