This document provides an overview of plant tissue culture and its applications. It discusses the history of plant tissue culture, fundamental principles such as totipotency and plasticity, common techniques like micropropagation and somatic cell genetics, major steps in the tissue culture process, and types of tissue culture including cell, callus, and organ culture. Applications of tissue culture covered include clonal propagation, production of secondary metabolites, genetic variability, somatic embryogenesis, haploid plant production, somatic hybridization, transgenic plants, and germplasm conservation. The document also discusses the history, rationale, ideal properties, plants used for, and target pathogens of edible vaccines - which involve expressing vaccine antigens in food crops to provide a low-cost,
Much faster rates of growth can be induced in vitro than by traditional means.
Multiplication of plants which are very difficult to propagate by cuttings or other traditional methods.
Production of large numbers of genetically identical clones in a short time
Seeds can be germinated with no risk of damping off/ predation.
Under certain conditions, plant material can be stored in vitro for considerable periods of time with little or no maintenance
Tissue culture techniques are used for virus eradication, genetic manipulation, somatic hybridization and other procedures that benefit propagation, crop improvement, and basic research.
By means of tissue culture it is possible to produce pathogen free plantlets by mass multiplication in a very limited amount of area from a very small sterile part of a mother plant. This method is also used to produce/ multiply plants that are to be transported across national border and so for their faster multiplication.But the establishment of a tissue culturing unit needs huge financial investments, skilled labors/technicians and required areas for its establishment are major constraints. Plant tissues grow and multiply in the labs only when there is an uncompetitive, growing condition with uninterrupted supply of nutrients.
Medium:
It contains all the elements that contribute the required nutrients that aid to the growth of the tissues; it is in liquid state or semi-solid in nature. The tissues are grown on the media. It consists of 95% of water, major and minor nutrients, plant growth hormones, vitamins, sugar rich compounds and chelating agents.
Totipotency:
It is the ability of a tissue or an organ of a plant to produce the whole plant, under the optional laboratory conditions and this is called as Totipotency. This is the baseline over which plant tissue culture relies upon.
Callus Culture:
When the cells divide into an undifferentiated mass it is called as callus. Any part of a plant can be used to produce the calli. It may be a stem, leaf, meristem or any other part. It is used to produce variations among the plantlets.
Suspension culture:
The callus produced from the explants are grown on nutrient solutions (that are semi solid) for a period of time and they are induced to produce plants with new traits.
Embryo Culture:
The method of culturing mature and immature embryos in media is called embryo culture. By this method, it is possible to produce plants from dormant seeds and seeds with metabolites that inhibit germination. This method is very important in crop improvement programs.
Somatic Embryogenesis:
When the plants are grown on nutrient media, calli are formed. When these calli are subjected to growth in cytokinin medium, somatic embryos are formed. They are circular, elongated,
Single cell culture
• As stated earlier, cells derived from a single cell through mitosis constitute a clone and the process of obtaining clones is called cloning (asexual progeny of a single individual make up.
Micropropagation and commercial exploitation in horticulture cropsDheeraj Sharma
Micro-propagation – principles and concepts, commercial exploitation in horticultural crops. Techniques - in vitro clonal propagation, direct organogenesis, embryogenesis, micrografting, meristem culture. Hardening, packing and transport of micro-propagules.
Much faster rates of growth can be induced in vitro than by traditional means.
Multiplication of plants which are very difficult to propagate by cuttings or other traditional methods.
Production of large numbers of genetically identical clones in a short time
Seeds can be germinated with no risk of damping off/ predation.
Under certain conditions, plant material can be stored in vitro for considerable periods of time with little or no maintenance
Tissue culture techniques are used for virus eradication, genetic manipulation, somatic hybridization and other procedures that benefit propagation, crop improvement, and basic research.
By means of tissue culture it is possible to produce pathogen free plantlets by mass multiplication in a very limited amount of area from a very small sterile part of a mother plant. This method is also used to produce/ multiply plants that are to be transported across national border and so for their faster multiplication.But the establishment of a tissue culturing unit needs huge financial investments, skilled labors/technicians and required areas for its establishment are major constraints. Plant tissues grow and multiply in the labs only when there is an uncompetitive, growing condition with uninterrupted supply of nutrients.
Medium:
It contains all the elements that contribute the required nutrients that aid to the growth of the tissues; it is in liquid state or semi-solid in nature. The tissues are grown on the media. It consists of 95% of water, major and minor nutrients, plant growth hormones, vitamins, sugar rich compounds and chelating agents.
Totipotency:
It is the ability of a tissue or an organ of a plant to produce the whole plant, under the optional laboratory conditions and this is called as Totipotency. This is the baseline over which plant tissue culture relies upon.
Callus Culture:
When the cells divide into an undifferentiated mass it is called as callus. Any part of a plant can be used to produce the calli. It may be a stem, leaf, meristem or any other part. It is used to produce variations among the plantlets.
Suspension culture:
The callus produced from the explants are grown on nutrient solutions (that are semi solid) for a period of time and they are induced to produce plants with new traits.
Embryo Culture:
The method of culturing mature and immature embryos in media is called embryo culture. By this method, it is possible to produce plants from dormant seeds and seeds with metabolites that inhibit germination. This method is very important in crop improvement programs.
Somatic Embryogenesis:
When the plants are grown on nutrient media, calli are formed. When these calli are subjected to growth in cytokinin medium, somatic embryos are formed. They are circular, elongated,
Single cell culture
• As stated earlier, cells derived from a single cell through mitosis constitute a clone and the process of obtaining clones is called cloning (asexual progeny of a single individual make up.
Micropropagation and commercial exploitation in horticulture cropsDheeraj Sharma
Micro-propagation – principles and concepts, commercial exploitation in horticultural crops. Techniques - in vitro clonal propagation, direct organogenesis, embryogenesis, micrografting, meristem culture. Hardening, packing and transport of micro-propagules.
MEDICINAL PLANT BIOTECHNOLOGY UNIT 2, MPG, SEM 2. NOTES Different tissue culture techniques: Organogenesis and embryogenesis, synthetic seed and monoclonal variation
Protoplast fusion, Hairy root multiple shoot cultures and their applications.
Micro propagation of medicinal and aromatic plants.
Sterilization methods involved in tissue culture, gene transfer in plants and their applications.
Plant biotechnology also known as green biotechnology is the use of biotechnology in plant or crop production. There are several techniques used such as ell culturing. Organ culture, explant culture, cell suspension culture are some culture types. This is a very useful technology in which have several applications like synthetic seed production, somaclonal variation, cybridization, hybridization.
It gives the general knowledge about plant tissue culture. As this topic is an important aspects of plant biotechnology, it will remind a brief idea about why it is necessary.
Defecation
Normal defecation begins with movement in the left colon, moving stool toward the anus. When stool reaches the rectum, the distention causes relaxation of the internal sphincter and an awareness of the need to defecate. At the time of defecation, the external sphincter relaxes, and abdominal muscles contract, increasing intrarectal pressure and forcing the stool out
The Valsalva maneuver exerts pressure to expel faeces through a voluntary contraction of the abdominal muscles while maintaining forced expiration against a closed airway. Patients with cardiovascular disease, glaucoma, increased intracranial pressure, or a new surgical wound are at greater risk for cardiac dysrhythmias and elevated blood pressure with the Valsalva maneuver and need to avoid straining to pass the stool.
Normal defecation is painless, resulting in passage of soft, formed stool
CONSTIPATION
Constipation is a symptom, not a disease. Improper diet, reduced fluid intake, lack of exercise, and certain medications can cause constipation. For example, patients receiving opiates for pain after surgery often require a stool softener or laxative to prevent constipation. The signs of constipation include infrequent bowel movements (less than every 3 days), difficulty passing stools, excessive straining, inability to defecate at will, and hard feaces
IMPACTION
Fecal impaction results from unrelieved constipation. It is a collection of hardened feces wedged in the rectum that a person cannot expel. In cases of severe impaction the mass extends up into the sigmoid colon.
DIARRHEA
Diarrhea is an increase in the number of stools and the passage of liquid, unformed feces. It is associated with disorders affecting digestion, absorption, and secretion in the GI tract. Intestinal contents pass through the small and large intestine too quickly to allow for the usual absorption of fluid and nutrients. Irritation within the colon results in increased mucus secretion. As a result, feces become watery, and the patient is unable to control the urge to defecate. Normally an anal bag is safe and effective in long-term treatment of patients with fecal incontinence at home, in hospice, or in the hospital. Fecal incontinence is expensive and a potentially dangerous condition in terms of contamination and risk of skin ulceration
HEMORRHOIDS
Hemorrhoids are dilated, engorged veins in the lining of the rectum. They are either external or internal.
FLATULENCE
As gas accumulates in the lumen of the intestines, the bowel wall stretches and distends (flatulence). It is a common cause of abdominal fullness, pain, and cramping. Normally intestinal gas escapes through the mouth (belching) or the anus (passing of flatus)
FECAL INCONTINENCE
Fecal incontinence is the inability to control passage of feces and gas from the anus. Incontinence harms a patient’s body image
PREPARATION AND GIVING OF LAXATIVESACCORDING TO POTTER AND PERRY,
An enema is the instillation of a solution into the rectum and sig
MEDICINAL PLANT BIOTECHNOLOGY UNIT 2, MPG, SEM 2. NOTES Different tissue culture techniques: Organogenesis and embryogenesis, synthetic seed and monoclonal variation
Protoplast fusion, Hairy root multiple shoot cultures and their applications.
Micro propagation of medicinal and aromatic plants.
Sterilization methods involved in tissue culture, gene transfer in plants and their applications.
Plant biotechnology also known as green biotechnology is the use of biotechnology in plant or crop production. There are several techniques used such as ell culturing. Organ culture, explant culture, cell suspension culture are some culture types. This is a very useful technology in which have several applications like synthetic seed production, somaclonal variation, cybridization, hybridization.
It gives the general knowledge about plant tissue culture. As this topic is an important aspects of plant biotechnology, it will remind a brief idea about why it is necessary.
Defecation
Normal defecation begins with movement in the left colon, moving stool toward the anus. When stool reaches the rectum, the distention causes relaxation of the internal sphincter and an awareness of the need to defecate. At the time of defecation, the external sphincter relaxes, and abdominal muscles contract, increasing intrarectal pressure and forcing the stool out
The Valsalva maneuver exerts pressure to expel faeces through a voluntary contraction of the abdominal muscles while maintaining forced expiration against a closed airway. Patients with cardiovascular disease, glaucoma, increased intracranial pressure, or a new surgical wound are at greater risk for cardiac dysrhythmias and elevated blood pressure with the Valsalva maneuver and need to avoid straining to pass the stool.
Normal defecation is painless, resulting in passage of soft, formed stool
CONSTIPATION
Constipation is a symptom, not a disease. Improper diet, reduced fluid intake, lack of exercise, and certain medications can cause constipation. For example, patients receiving opiates for pain after surgery often require a stool softener or laxative to prevent constipation. The signs of constipation include infrequent bowel movements (less than every 3 days), difficulty passing stools, excessive straining, inability to defecate at will, and hard feaces
IMPACTION
Fecal impaction results from unrelieved constipation. It is a collection of hardened feces wedged in the rectum that a person cannot expel. In cases of severe impaction the mass extends up into the sigmoid colon.
DIARRHEA
Diarrhea is an increase in the number of stools and the passage of liquid, unformed feces. It is associated with disorders affecting digestion, absorption, and secretion in the GI tract. Intestinal contents pass through the small and large intestine too quickly to allow for the usual absorption of fluid and nutrients. Irritation within the colon results in increased mucus secretion. As a result, feces become watery, and the patient is unable to control the urge to defecate. Normally an anal bag is safe and effective in long-term treatment of patients with fecal incontinence at home, in hospice, or in the hospital. Fecal incontinence is expensive and a potentially dangerous condition in terms of contamination and risk of skin ulceration
HEMORRHOIDS
Hemorrhoids are dilated, engorged veins in the lining of the rectum. They are either external or internal.
FLATULENCE
As gas accumulates in the lumen of the intestines, the bowel wall stretches and distends (flatulence). It is a common cause of abdominal fullness, pain, and cramping. Normally intestinal gas escapes through the mouth (belching) or the anus (passing of flatus)
FECAL INCONTINENCE
Fecal incontinence is the inability to control passage of feces and gas from the anus. Incontinence harms a patient’s body image
PREPARATION AND GIVING OF LAXATIVESACCORDING TO POTTER AND PERRY,
An enema is the instillation of a solution into the rectum and sig
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Antibiotic Stewardship by Anushri Srivastava.pptxAnushriSrivastav
Stewardship is the act of taking good care of something.
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
WHO launched the Global Antimicrobial Resistance and Use Surveillance System (GLASS) in 2015 to fill knowledge gaps and inform strategies at all levels.
ACCORDING TO apic.org,
Antimicrobial stewardship is a coordinated program that promotes the appropriate use of antimicrobials (including antibiotics), improves patient outcomes, reduces microbial resistance, and decreases the spread of infections caused by multidrug-resistant organisms.
ACCORDING TO pewtrusts.org,
Antibiotic stewardship refers to efforts in doctors’ offices, hospitals, long term care facilities, and other health care settings to ensure that antibiotics are used only when necessary and appropriate
According to WHO,
Antimicrobial stewardship is a systematic approach to educate and support health care professionals to follow evidence-based guidelines for prescribing and administering antimicrobials
In 1996, John McGowan and Dale Gerding first applied the term antimicrobial stewardship, where they suggested a causal association between antimicrobial agent use and resistance. They also focused on the urgency of large-scale controlled trials of antimicrobial-use regulation employing sophisticated epidemiologic methods, molecular typing, and precise resistance mechanism analysis.
Antimicrobial Stewardship(AMS) refers to the optimal selection, dosing, and duration of antimicrobial treatment resulting in the best clinical outcome with minimal side effects to the patients and minimal impact on subsequent resistance.
According to the 2019 report, in the US, more than 2.8 million antibiotic-resistant infections occur each year, and more than 35000 people die. In addition to this, it also mentioned that 223,900 cases of Clostridoides difficile occurred in 2017, of which 12800 people died. The report did not include viruses or parasites
VISION
Being proactive
Supporting optimal animal and human health
Exploring ways to reduce overall use of antimicrobials
Using the drugs that prevent and treat disease by killing microscopic organisms in a responsible way
GOAL
to prevent the generation and spread of antimicrobial resistance (AMR). Doing so will preserve the effectiveness of these drugs in animals and humans for years to come.
being to preserve human and animal health and the effectiveness of antimicrobial medications.
to implement a multidisciplinary approach in assembling a stewardship team to include an infectious disease physician, a clinical pharmacist with infectious diseases training, infection preventionist, and a close collaboration with the staff in the clinical microbiology laboratory
to prevent antimicrobial overuse, misuse and abuse.
to minimize the developme
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The dimensions of healthcare quality refer to various attributes or aspects that define the standard of healthcare services. These dimensions are used to evaluate, measure, and improve the quality of care provided to patients. A comprehensive understanding of these dimensions ensures that healthcare systems can address various aspects of patient care effectively and holistically. Dimensions of Healthcare Quality and Performance of care include the following; Appropriateness, Availability, Competence, Continuity, Effectiveness, Efficiency, Efficacy, Prevention, Respect and Care, Safety as well as Timeliness.
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Alongside the 77th World Health Assembly in Geneva on 28 May 2024, we launched the second version of our Index, allowing us to track progress and give new insights into what needs to be done to keep populations healthier for longer.
The speakers included:
Professor Orazio Schillaci, Minister of Health, Italy
Dr Hans Groth, Chairman of the Board, World Demographic & Ageing Forum
Professor Ilona Kickbusch, Founder and Chair, Global Health Centre, Geneva Graduate Institute and co-chair, World Health Summit Council
Dr Natasha Azzopardi Muscat, Director, Country Health Policies and Systems Division, World Health Organisation EURO
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Dr Shyam Bishen, Head, Centre for Health and Healthcare and Member of the Executive Committee, World Economic Forum
Dr Karin Tegmark Wisell, Director General, Public Health Agency of Sweden
2. Contents:
• History
• Introduction
• Tissue culture
• Nutrient medium
• Fundamental Principles of
plant tissue culture
• Plant tissue culture Techniques
• Micropropagation
• Somatic cell Genesis
• Transgenic Plants
• Major steps of Tissue Culture
• Initiation phase
• Multiplication phase
• Root formation phase
• Types of Tissue culture
• Cell culture
• Callus culture
• Protoplast culture
• Organ culture
• Seed culture
• Embryo culture
• Meristem culture
• Anther culture
• Applications of Tissue
Culture
3. History
• Haberlandt, Father of plant tissue culture. cultured single
cells on Knop’s salt solution.
• Hanning(1904) Embryo culture of selected
crucifers.
• Robbins(1922), in vitro culture of root tips.
• Muir(1953) isolation and culture of single plant cells.
• Skoog and Miller(1957) hormonal control in tissue culture.
• Reinert and Steward(1958) report somatic embryogenesis.
4. History
• E C Cocking (1960) Isolation of
enzymatic degradation method.
• Murashige and Skoog (1962)
media.
protoplasts by
Development of MS
• Guha and Maheshwari (1964) Production of First
haploid plant by anther culture
5. Plant Tissue Culture
• The growth or regeneration of plant cells,
tissues, organs or whole plants in artificial
medium under aseptic conditions.
• In this technique use
called Explant.
plant parts or cells
INTRODUCTION:
• The most widely used artificial nutrient
medium is MS medium.
6. Fundamental Principles of Plant Tissue Culture
• Totipotency, ability of plant cells to regenerate
into whole new plant
• Plasticity, ability of plants to alter their
metabolism, growth and development to best
suit their environment
Plant cell
Plant
tissue
culture
depends
upon;
Tissue
Callus
Plant organ Embryo
New plant
7. Plant Tissue Culture Techniques:
1. Micropropagation:
• Developing high- quality clonal plants.
• Provide rapid and large scale propagation of new
genotypes.
• Steps:
• Sterilization and introduction
• Shoot production
• Root production
• Transfer to soil
8. 2. Somatic cell genetics:
• It is used mostly in terms of haploid production
and somatic hybridization.
• Steps:
• Isolation of protoplast
• Fusion by using chemicals
• Plating of fused protoplast
• Selection
9. 3. Transgenic plants:
• Transgenic plants are plants that have been
genetically engineered, uses recombinant DNA
techniques to create plants with new
characteristics.
•They are identified as a class of genetically
modified organism (GMO).
•The complete process is
described as:
10. Major Steps of Tissue Culture:
• Initiation Phase (Stage 1)
• The tissue of interest is obtained,
and introduced.
• Multiplication Phase (Stage 2)
• The in vitro plant material is introduced in to
the medium.
• Proliferation of the tissue and the production
of multiple shoots.
sterilized
11. Major Steps of Tissue Culture:
• Root formation (Stage 3)
• Roots are formed.
• Here, hormones are required in order to
induce rooting such as “Auxin”.
• Consequently complete plantlets.
13. Cell culture:
• The culture of isolated individual cells.
• Carried out in dispersion medium.
Callus culture:
• The growth of callus from explant by
providing appropriate conditions.
• Darkness and solid medium gelled by agar
stimulate callus formation.
14. Protoplast culture:
• The cell in which the cell wall has been removed.
• In this, isolated protoplasts are cultured on a
suitable medium under the aseptic condition.
• The protoplast culture is aimed to develop
genetically transformed plant.
15. Organ culture:
• Isolated plant organs (Shoot, root, leaf, and flower) are
used as explant.
• The organ culture may be organized or unorganized.
• New growth (differentiated structures)
continues given that the organ retains its:
• Physiological features,
• Provide information on patterns of growth,
• As well as development.
16. Seed culture:
• For this method, explants are obtained from an in-vitro
derived plant.
• Introduced into an artificial environment.
• Where they get to proliferate.
• Seed culture is primarily used for plants such as orchids
17. Embryo Culture:
• Embryo culture is the isolation and growth of
mature or immature embryo in-vitro.
• For embryo culture, the ovule, seed or fruit
from which the embryo is to be obtained is
sterilized.
• Culturing them in nutrient media, help in
developing viable seedlings.
18. Meristem culture:
• A meristem is a localized group of cells, which
are actively dividing and give rise to
permanent tissue.
• The apical meristem of
cultured.
shoots is
• To get the disease-free plants.
19. Anther Culture:
• Anthers of some plants are cultured on a
suitable medium to produce haploid plants,
it is called anther culture.
• Separated anthers from flowers.
• Cultured on a suitable medium.
• This technique was first used in India to
produce haploids of Datura.
21. Clonal Propagation:
• Useful tool to get a large population of
species having desirable traits.
plant
• This technique is very much used in horticulture
and silviculture—orchids and many fruit plants.
• production of plants of same
Large scale
genetic stock.
22. Secondary Metabolites:
• Secondary metabolites produced by
cultures are rather small in amount.
plant cell
• By clonal selection the particular high yielding
clone of cells can be isolated.
• Production of many useful compounds
can be done by plant cell culture.
23. Genetic Variability:
• The chromosomal instability in the cultured
cells play an important role in polyploidization
of cells and genetically variable plants can be
raised.
•
• Such kind of variations may show some useful
characters such as:
Different kinds of morphological variations in leaf
and flowering.
24. Somatic Embryogenesis and Synthetic Seed:
• Direct or indirect somatic embryogenesis may be
achieved from:
• Pro-embryonic cell of the direct explant
• The embryoids developed withinthe callus
tissue.
• The application of this technique is:
• the mass production of adventitious embryos
which ultimately develop into complete
plantlet.
25. Haploid Plants:
• Haploid plants can be obtained through anther
pollen culture (androgenesis).
• Colchicine treatment within a very short period.
or
• These haploids produced homozygous diploid or
polyploid lines.
26. Somatic Hybrids:
hybridization successful.
• Obtain somatic hybrid plants between sexually compatible
and incompatible plants.
• Production of cybrid is also of immense
importance in the plant breeding program.
• Somatic hybridization, using isolated
somatic
protoplasts is a new tool to make
the wide
27. Transgenic Plants:
• The genetically modified (GM) plants, in which a
functional foreign gene has been incorporated by
biotechnological method.
• A number of transgenic plants
have been produced carrying genes for different
traits.
• The direct DNA uptake through protoplast is
the most ideal method.
28. Germplasm Conservation:
• Many of the important crop species produce recalcitrant
seeds.
• Mainly
needed
the plant species which are endangered,
to be conserved by ex-situ method of
germplasm conservation.
• Plant tissue culture may be applied for this purpose.
30. VACCINE
• A biological preparation that improves immunity to a particular disease.
• contains an agent that resembles a disease-causing microorganism and is
often made from weakened or killed forms of the microbe
• stimulate the body's immune system to recognize the agent, destroy it, and
keep a record of it for later encounters
• reduced mortality rate caused by various organisms.
• one of the safe and effective measure to control various infectious diseases.
• A protein which acts as the vaccine, present in food and consumed as the
internal composition of food is known as EDIBLE VACCINE
31. EDIBLE VACCINE- WHY?
• Immunization through DNA vaccines is an alternative but is an expensive
approach
• Edible vaccine gives cost-effective, easy-to-administer, easy-to-store and
socio-culturally readily acceptable vaccines for their delivery systems.
• Oral vaccines provide “mucosal immunity” at various sites by secreting
antibodies.
• Don’t need to worry about re-use, misuse and lack of sterilization. Thus,
low risk of infection.
32. History of Edible vaccines
Mason et al 1992 Haq et al 1995 McGarvey et al 1995 Mason et al 1996
Hepatitis
Hepatitis B
surface antigen
(HBsAg)
Tobacco/leaf
Norwalk virus
(NV)
Gastroenteritis
Norwalk virus
capsid protein
(NVCP)
Potato/tuber
tobacco/leaf
Rabies virus
Rabies
Rabies virus
glycoprotein
(RVG)
Tomato/leaf,
fruit
V. Cholerae
Cholera
Cholera toxin
B subunit
(CTB)
Tobacco/leaf
Hein et al 1996 ….
E. Coli
Diarrhea
Heat labile
toxin B subunit
(LTB)
Potato/tuber,
tobacco/
leaf
5
33. IDEAL PROPERTIES
EDIBLE
VACCINES
Nontoxic or
Nonpathogenic
very low levels of
side effects
Not
problems
cause
in
individuals with
impaired
immune system
Long lasting humoral
and cellular immunities
Vaccination
should be
Simple
Not
contaminate
the
Environment
Sould be
effective in
affordable
35. Plant species
Banana
Advantages
Do not need cooking.
Protein not destroyed even after cooking.
Inexpensive .
Grown widely in developing countries.
Disadvantages
Trees take 2-3 to mature years. Spoils
rapidly after ripening.
Potato :
Advantage
Easily transformed. Easily
propagated.
Stored for long periods without
refrigeration.
Disadvantage
Need cooking which denature antigen.
Rice
Advantages
Commonly used in baby food. High
expression of antigen.
Disadvantages
Grows slowly.
Requires glasshouse condition.
Tomato
Advantage
Grow quickly. Cultivate broadly.
High content Vitamin-A may boost
immune response.
Disadvantages
Spoils readily.
36. TARGET PATHOGENS EXPRESSED IN MODE OF ADMINESTRATION
Enterotoxigenic Ecoli (humans) Potato, tobacco Immunogenic and protective when
administered orally.
Vibrio cholera(humans) Potato Immunogenic and protective when
administered orally.
Hepatitis B virus Tobacco Extracted proteins is Immunogenic
(humans) when administered by injection
Hepatitis B virus (humans) Potato Immunogenic and protective when
administered orally.
Norwalk virus(humans) Potato Virus like particles form and
Immunogenic when administered orally.
Rabies virus (humans) Tomato Intact glycoproteins
Foot and mouth disease (agricultural
domestic animals)
Arabidopsis Immunogenic and protective when
administered orally
Foot and mouth disease (agricultural
domestic animals)
Alfalfa Immunogenic and protective when
administered by injection or orally
Transmissible Maize Protective when administered
gastroenteritis corona virus oral
37. recombinant DNA technology
A technique mainly used to change the phenotype of an
organism (host) when a genetically altered vector is
introduced and integrated into the genome of the
organism.
So, basically, this process involves the introduction of a
foreign piece of DNA structure into the genome which
contains our gene of interest.
This gene which is introduced is the recombinant gene
and the technique is called the recombinant DNA
technology.
42. CONCEPT OF EDIBLE VACCINE
Developed by Arntzen in the 1990s.
Introduce genes of interest into plants (Transformation)
Genes expressed in the plant tissues edible parts (Transgenic plants)
Genes encode putatively protective vaccine antigens from viral, bacterial, and
parasitic pathogens that cause disease in humans and animals
Ingestion of the edible part of the transgenic plant (Oral delivery of vaccine)
46. 1. MALARIA
Three antigens
development
are currently being investigated for the
of a plant-based malaria vaccine,
merozoite surface protein (MSP) 4 and MSP 5 from
Plasmodium falciparum, and MSP 4/5 from P. yoelli.
Wang et al have demonstrated that oral immunization
of mice with recombinant MSP 4, MSP 4/5 and MSP1,
a mucosal adjuvant,
effective against blood-
co-administered with CTB as
induced antibody responses
stage parasite.
2. MEASLES
Mice fed with tobacco expressing MV-H (measles
haemagglutinin from Edmonston strain) could
virus
attain
antibody titers five times the level considered protective for
humans and they also demonstrated secretory IgA in their
faeces.
Carrot, banana and rice are the potential candidates
47. 3. HEPATITIS B
significantly exceeded the protective level
humans..
of 10 mIU/mL in
potato-based vaccine against hepatitis B have reported The
amount of HBsAg needed for one dose could be achieved in a
single potato.
4. STOPPING AUTOIMMUNITY
The transgenic potato and tobacco plants when
fed to nonobese diabetic mice showed increased
IgG, an antibody associated with
that suppress harmful immune
levels of
cytokines
response.
48. 5. CHOLERA
plants were transformed with the gene
encoding B subunit of the E. coli heat liable
enterotoxin (LT-B). Transgenic potatoes
expressing LT-B were found to induce both
serum and secretory antibodies when fed to
mice; these antibodies were
protective in bacterial toxin assay
in vitro. This is the first “proof of
concept” for the edible vaccine.
49. Advantages of Edible vaccines
DO not require administration by injection.
Possible production of vaccines with low costs.
Do not require separation and purification of
vaccines from plant materials.
Necessary syringe & needles not required.
Economical in mass production and
transportation.
Heat stable, eliminating the need for
refrigeration.
50. DISADVANTAGE OF EDIBLE VACCINE
Development of immunotolerance to vaccine peptide or protein.
Consistency of dosage form fruit to fruit, plant-to-plant, and generation-
to-generation is not similar.
Stability of vaccine in fruit is not known.
Dosage of vaccines would be variable.
Selection of best plant is difficult.
Certain foods like potato are not eaten raw, and cooking the food might
weakens the medicine present in it.
Not convenient for infants.
51. Safety aspects
• Contamination through cross pollination.
• vaccine antigen may affect browsing animals.
• Vaccine contamination via plant debris spreading on surfaces and
ground waters.
• Affect on humans living in the area drinking vaccine polluted water or
breathing vaccine polluted dust.
• cultivation and production of pharmaceutical crops should be limited to
control the production facilities like greenhouse, or in plant tissue culture,
that prevent the environmental release of biopharmaceuticals.