This document summarizes a study on the effect of partially replacing concentrate mixture with sprouted wheat and maize fodder on milk yield and quality in lactating cows. Nine crossbred cows were divided into three groups fed different levels of replacement: Group A received 25% replacement, Group B received 37.5% replacement, and Group C received the control diet. Milk yield increased the most (15.37%) for Group A in Phase 2 with 37.5% replacement. Milk fat, protein and total solids also increased more for Groups A and B compared to the control Group C across phases. The study concluded that partially replacing concentrate mixture with hydroponic wheat and maize fodder up to 37.5%

1. WELCOME

2. EFFECT OF PARTIAL REPLACEMENT OF CONCENTRATE
MIXTURE BY SPROUTED WHEAT AND MAIZE FODDER
ON MILK YIELD AND MILK QUALITY OF LACTATING
COWS
Presented by
MD. MAZHARUL ISLAM
ID No.: 15 DS JJ 01 M
Reg. No.: 36518; Session: 2009-2010
Supervisor
Prof. Dr. MD. NURUL ISLAM
Professor
Dept. of DAIRY SCIENCE
Co-Supervisor
Dr. MOHAMMAD SHOHEL RANA
SIDDIKI
Associate Professor
Dept. of DAIRY SCIENCE
Department of Dairy Science
Bangladesh Agricultural University
Mymensingh-2202

3. Introduction
Scarcity
Increasing human population
Industrialization
Limited land
Limited land for fodder cultivation
Feeding of fodders
Increases animal productivity
Profitability of dairy farmers.
Deficient in Milk production
 Produce 6.97 MMT of Milk annually against
the requirement of appx. 14.45MMT

4. SPROUTED FODDER/HYDROPONIC FODDER??
 Derived from the Greek word ‘Hydro’ means ‘water’ and
‘ponic’ means ‘working’
 It is a method of growing plants without soil.
 Hydroponic fodder is also known as ‘sprouted fodder’
Advantages:
 Shorter period of time (8-10 days)
 Smaller area than traditionally-grown crops
 Year-round growing system, produces a consistent
quantity and quality of plant material

5. The following objectives are undertaken
To study the feasibility of partial replacement of
conventional concentrate mixture by sprouted
fodder (wheat and maize) in lactating crossbred
cows ration.
To study the effect of feeding different levels of
sprouted fodder (wheat and maize) on milk
yield and milk quality of lactating crossbred
cows.

6. Materials and Method
Experimental Period
 10th November’15 to 20th January’16 (Fodder production, Feeding
trial, Laboratory analysis)
 Divided into three phases: 3 weeks in each phase
Experimental Site
 Fodder production: Faculty of Fisheries.
 Feeding trial : Bangladesh Agricultural University Dairy Farm .
 Laboratory analysis : Dairy Technology and Microbiology
Laboratory.
Experimental Animals
 Selected nine lactating crossbred cows(Holstein Friesian)
 Divided into three groups having three cows in each
 Groups are represented as A, B and C

7. Experimental Diet Phase-1
Group-A Group-B Group-C
Concentrate
Mixture
3 kg 3 kg 4 kg
Sprouted Fodder 1kg sprouted wheat
(25% replacement)
1kg sprouted Maize
(25% replacement)
No sprouted
fodder
Phase-2
Concentrate
Mixture
2.5 kg 2.5 kg 4kg
Sprouted Fodder
1.5kg sprouted wheat
(37.5% replacement)
1.5kg sprouted
Maize (37.5%
replacement)
No sprouted
fodder
Phase-3
Concentrate
Mixture
2 kg 2kg 4kg
Sprouted Fodder
2kg sprouted wheat
(50% replacement)
2kg sprouted Maize
(50% replacement)
No sprouted
fodder
In all three phases green grass were given ad libitum basis.
Layout
OF
FEEDING
TRIAL

8. Results

9. Average Milk yield result (l/d/h) in whole three phases
Milk yield in different phases Experimental Groups
Group A
(mean±SD)
Group B
(mean±SD)
Group C
(mean±SD)
Phase -1
7.32±0.14 8.15±0.72 6.73±1.13
Initial record 6.70±0.10 7.75±0.75 6.85±1.15
% Change 9.25± 0.04 5.16± 0.04 1.75± 0.04
Phase -2 7.72±0.12 8.50±1.00 6.75±1.32
Initial record 6.70±0.10 7.75±0.75 6.85±1.15
% Change 15.37± 0.02 9.67± 0.25 1.45± 0.17
Phase -3 7.70±0.10 8.43±1.03 6.40±1.20
Initial record 6.70±0.10 7.75±0.75 6.85±1.15
% Change 15.07± 0.01 8.77± 0.23 6.5± 0.50

10. Average milk yield (l/d) in each group of cows in different phases
0
1
2
3
4
5
6
7
8
9
10
Group A Group B Group C
Avg.milkyield(l/d)
Treated groups
Phase 1 Phase 2 Phase 3

11. Average Milk Fat content (g/kg) in different groups of
cows
0
10
20
30
40
50
60
p-1 p-2 p-3
Fatcontent(g/kg)
Different phases
A B C

12. Average Milk Protein content (g/kg) in different group of
cows
2.95
3
3.05
3.1
3.15
3.2
3.25
3.3
P-1 P-2 P-3
Different phases
A B C

13. Average Milk Total Solids content (g/kg) in different group
of cows
110
115
120
125
130
135
140
P-1 P-2 P-3
TScontent,(g/kg)
Different phases
A B C

14. Average chemical parameters of milk samples collected during
experimental period in ‘phase-1’
Chemical
parameters
Experimental Groups Levels of
significanceGroup A
(mean±SD)
Group B
(mean±SD)
Group C
(mean±SD)
Specific gravity 1.030±0.00 1.032±0.00 1.033±0.00 NS
Acidity (%) 0.15±0.01 0.15±0.01 0.16±0.00 NS
Fat (g/kg) 47.7a±0.18 42.9b±0.34 42.7b±0.08 *
Protein (g/kg) 32.2±0.01 31.8±0.06 31.9±0.08 NS
Lactose (g/kg) 45.2±0.04 46.2±0.12 46.3±0.11 NS
TS (g/kg) 131.5a±0.10 127.4b±0.16 128.1b±0.12 *
SNF (g/kg) 84.0±0.06 84.6±0.18 85.3±0.20 NS
Ash (g/kg) 6.6±0.01 6.5±0.01 6.7±0.02 NS
* 5% significance (p<0.05), abc = mean value with different superscripts letter within a row differed
significantly.

15. Average chemical parameters of milk samples collected during
experimental period in ‘phase-2’
Chemical
parameters
Experimental Groups Levels of
significanceGroup A
(mean±SD)
Group B
(mean±SD)
Group C
(mean±SD)
Specific gravity 1.029±0.00 1.031±0.00 1.032±0.00 NS
Acidity (%) 0.16±0.01 0.15±0.01 0.15±0.01 NS
Fat (g/kg) 50.6a±0.03 44.2b±0.34 44.4b±0.18 *
Protein (g/kg) 32.3a±0.03 31.6b±0.06 30.9b±0.06 *
Lactose (g/kg) 46.1±0.18 44.0±0.11 44.6±0.13 NS
TS (g/kg) 134a.4±0.17 125.6b±0.22 124.5b±0.13 *
SNF (g/kg) 83.8±0.20 81.3±0.13 82.5±0.20 NS
Ash (g/kg) 6.4±0.00 6.4±0.01 6.6±0.02 NS
* 5% significance (p<0.05), abc = mean value with different superscripts letter within a row
differed significantly.

16. Average chemical parameters of milk samples collected during
experimental period in ‘phase-3’
Chemical
parameters
Experimental Groups Levels of
significanceGroup A
(mean±SD)
Group B
(mean±SD)
Group C
(mean±SD)
Specific gravity 1.029±0.00 1.030±0.00 1.032±0.00 NS
Acidity (%) 0.16±0.00 0.16±0.00 0.16±0.01 NS
Fat (g/kg) 50.3a±0.1 45.4b±0.36 42.3c±0.03 *
Protein (g/kg) 32.4a±0.02 32.3ab±0.05 31.4b±0.02 *
Lactose (g/kg) 45.6±0.09 46.4±0.06 45.6±0.05 NS
TS (g/kg) 134.9a±0.04 120.7c±0.31 126.2b±0.09 *
SNF (g/kg) 84.6±0.14 85.2±0.06 83.9±0.07 NS
Ash (g/kg) 6.5±0.01 6.6±0.01 6.7±0.00 NS
* 5% significance (p<0.05), abc = mean value with different superscripts letter within a row
differed significantly.

17. Conclusion
In case of 37.5% replacement of conc. mixture
by sprouted Wheat and Maize fodder gives
result of higher percentage milk yield.
 The Milk Fat, Protein and Total solids content
are also increase when sprouted Wheat and
Maize fodder partially replaced with conc.
mixture.
 Feeding addition of hydroponic fodder with
conc. mixture to dairy cows shows better
quantity of milk yield as well as milk quality.

18. T
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A
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