The document summarizes a presentation on antioxidant peptides derived from the blue-spotted stingray. It discusses alternative methods to obtain bioactive peptides from proteins, characterization of antioxidant capacity, identification of two antioxidant peptides (WAFAPA and MYPGLA) from the <3 kDa fraction of stingray hydrolysate, and characterization of their stability under thermal, pH, and simulated digestion conditions. The conclusion discusses the potential of antioxidant peptides for food and medical applications and need for further research.
Synbiotic effects of the Chitosana and Acinetobacter KU011TH on Hybrid catfis...piseysay2
Aquatic animal health management has become a crucial component in the goal of increasing catfish aquaculture
productivity. Additionally, hybrid catfish (Clarias gariepinus × C. macrocephalus) has been promoted as a highly
profitable freshwater fish in Asia. Interestingly, the crucial diseases induced by Aeromonas hydrophila have been
reported to greatly impede catfish production. To overcome this challenge, the aim was to investigate the effects
of the oral administration of potentially synbiotic chitosan (CH) and Acinetobacter KU011TH (AK) on the growth
performance, immunological responses, and disease resistance of hybrid catfish against A. hydrophila.
This research article studied the stability of vitamin C in homogenized fruits and vegetables stored at different temperatures. Samples of broccoli, potatoes, spinach, strawberries, oranges, and tomatoes were homogenized and stored under refrigeration (0-5°C), conventional freezing (-10 to -20°C), or ultra-low freezing (<-55°C) for up to 7 days. Vitamin C was most stable in samples stored at ultra-low freezing, with no decrease after 7 days. Under refrigeration, the largest decreases were seen in raw spinach and broccoli, losing an average of 9.5 mg/100g and 33.1 mg/100g respectively after 1 day. With conventional freezing, losses were
Chlorogenic acid (CGA): A potential Anti-obesity Phytochem...sudharani028
CHLOROGENIC ACID (CGA) belongs to the super family Caffeoylic Acid is gaining lot of media attention due to its anti-potential property. The word “Chloro" derived from Greek (light green) and Genic means "giving rise to”. You will get the information related to the anti-obesity phytochemical (CGA) and its mechanism in managing obesity
EFFECT OF MICROBIAL TRANSGLUTAMINASE (MTGASE) AND ISOLATED SOY PROTEIN (ISP) ...Hazmi Y
This study was carried out to determine the effects of Microbial Transglutaminase (MTGase) and isolated soy protein (ISP) on tilapia (Oreochromis niloticus) surimi gels. MTGase at 0, 0.3, 0.6 percent and ISP at 0, 3 and 6 percent were added to surimi gels of tilapia.
This document summarizes a study that evaluated the probiotic properties of microorganisms isolated from a honey-based kefir beverage. Three strains (Lactobacillus satsumensis LPBF1, Leuconostoc mesenteroides LPBF2, and Saccharomyces cerevisiae LPBF3) were tested for their ability to tolerate acid and bile salts, adhere to epithelial cells, inhibit pathogens, and protect DNA against oxidative damage. All three strains showed resistance to low pH and bile salts, adhesion to cells, and inhibition of E. coli and S. aureus. S. cerevisiae was most effective at protecting DNA against hydroxyl radical damage, likely due to its high antioxidant production.
The document summarizes a presentation on antioxidant peptides derived from the blue-spotted stingray. It discusses alternative methods to obtain bioactive peptides from proteins, characterization of antioxidant capacity, identification of two antioxidant peptides (WAFAPA and MYPGLA) from the <3 kDa fraction of stingray hydrolysate, and characterization of their stability under thermal, pH, and simulated digestion conditions. The conclusion discusses the potential of antioxidant peptides for food and medical applications and need for further research.
Synbiotic effects of the Chitosana and Acinetobacter KU011TH on Hybrid catfis...piseysay2
Aquatic animal health management has become a crucial component in the goal of increasing catfish aquaculture
productivity. Additionally, hybrid catfish (Clarias gariepinus × C. macrocephalus) has been promoted as a highly
profitable freshwater fish in Asia. Interestingly, the crucial diseases induced by Aeromonas hydrophila have been
reported to greatly impede catfish production. To overcome this challenge, the aim was to investigate the effects
of the oral administration of potentially synbiotic chitosan (CH) and Acinetobacter KU011TH (AK) on the growth
performance, immunological responses, and disease resistance of hybrid catfish against A. hydrophila.
This research article studied the stability of vitamin C in homogenized fruits and vegetables stored at different temperatures. Samples of broccoli, potatoes, spinach, strawberries, oranges, and tomatoes were homogenized and stored under refrigeration (0-5°C), conventional freezing (-10 to -20°C), or ultra-low freezing (<-55°C) for up to 7 days. Vitamin C was most stable in samples stored at ultra-low freezing, with no decrease after 7 days. Under refrigeration, the largest decreases were seen in raw spinach and broccoli, losing an average of 9.5 mg/100g and 33.1 mg/100g respectively after 1 day. With conventional freezing, losses were
Chlorogenic acid (CGA): A potential Anti-obesity Phytochem...sudharani028
CHLOROGENIC ACID (CGA) belongs to the super family Caffeoylic Acid is gaining lot of media attention due to its anti-potential property. The word “Chloro" derived from Greek (light green) and Genic means "giving rise to”. You will get the information related to the anti-obesity phytochemical (CGA) and its mechanism in managing obesity
EFFECT OF MICROBIAL TRANSGLUTAMINASE (MTGASE) AND ISOLATED SOY PROTEIN (ISP) ...Hazmi Y
This study was carried out to determine the effects of Microbial Transglutaminase (MTGase) and isolated soy protein (ISP) on tilapia (Oreochromis niloticus) surimi gels. MTGase at 0, 0.3, 0.6 percent and ISP at 0, 3 and 6 percent were added to surimi gels of tilapia.
This document summarizes a study that evaluated the probiotic properties of microorganisms isolated from a honey-based kefir beverage. Three strains (Lactobacillus satsumensis LPBF1, Leuconostoc mesenteroides LPBF2, and Saccharomyces cerevisiae LPBF3) were tested for their ability to tolerate acid and bile salts, adhere to epithelial cells, inhibit pathogens, and protect DNA against oxidative damage. All three strains showed resistance to low pH and bile salts, adhesion to cells, and inhibition of E. coli and S. aureus. S. cerevisiae was most effective at protecting DNA against hydroxyl radical damage, likely due to its high antioxidant production.
Five diets (diets incorporated with folic acid, vitamin B, vitamin C, vitamin/
mineral mix and control) were used to determine their effects on survival, digestive
enzyme activity, protein profiling and growth of striped snakehead Channa striatus
fingerlings. All vitamin diets showed significant difference in enzyme activity like
protease, amylase and lipase, when compared to that of control. The fingerlings fed
with vitamin C diet showed significantly (P<0.05) better performance in terms of
survival rate, weight gain and Specific Growth Rate (SGR) when compared to the rest
of the four diets. The electrophoretic studies revealed that high molecular weight
fractions were added up in muscle tissue of C. striatus fed with vitamin and mineral
supplemented diets.
This document presents a research proposal for evaluating the effect of different stocking densities on growth and physiological function of Ompok bimaculatus (Pabda) reared in a biofloc system. The study will examine growth, survival, water quality, physiological indices, and digestive enzymes of Pabda at stocking densities of 3, 6, and 9 fish/L in biofloc and clear water control systems over 70 days. Expected outcomes include optimizing stocking density for growth and physiology of Pabda in a biofloc system. A literature review supports the research gap on species diversification and effect of stocking density in biofloc systems.
This study investigated the effects of reducing Fsp27 (fat-specific protein 27) levels through antisense oligonucleotides (ASOs) in mouse models of obesity and insulin resistance. Mice fed a high-fat diet or leptin-deficient ob/ob mice were treated with Fsp27 ASOs. Partial reduction of Fsp27 resulted in decreased visceral fat, improved insulin sensitivity and blood glucose control, and changes in genes related to lipid metabolism. The results suggest that reducing FSP27 activity through ASOs could be a potential therapeutic approach for insulin resistance and obesity in patients.
Austin publishing group - Oral kefir grains supplementation improves metaboli...Austin Publishing Group
This study has a novel approach to investigate the effects of oral supplementation of kefir grains on metabolic improvement and the expression of the antioxidant enzymes Glutathione Peroxidase (GPx) and Catalase (CAT) of the liver in malnourished mice.
1) The document evaluates adding high protein DDGS (distiller's dried grain with solubles) feed to vaname shrimp cultivation to overcome AHPND (Acute Hepatopancreatic Necrosis Disease). Water quality parameters like temperature, pH, salinity and dissolved oxygen remained optimal with DDGS addition.
2) Bacterial counts like total vibrio and total bacteria were higher with DDGS, possibly due to the pond's history of AHPND infection. Shrimp in DDGS ponds showed lower survival rates, likely due to an AHPND outbreak from vibrio parahemolyticus bacteria persisting in the pond in a dormant state.
3) While DDGS did not negatively
Development of a Bioactive Food Additive for Controlling of Fungal GrowthIJEAB
Fresh foods have a great importance in human nutrition. However, they are marketed with greatly reduced shelf life mainly due to fungal spoilage. In this work, cell wall degrading enzymes produced by Trichoderma asperellum T00 (TCWDE) were immobilized onto cashew gum polysaccharide (CGP) in order to evaluate the potential use of this material as food additive aiming to increase the shelf life by inhibiting fungal growth. Results from factorial design (32) evidenced that the best conditions for TCWDE immobilization was achieved with 20 min of reaction using 1 mmol L-1 of NaIO4. On these conditions it was observed 91% of retention yield for NAGase (30.1 ± 0.38 U mL-1), 41% for chitinase (0.67 ± 0.05 U mL-1), and 24% for β-1,3-glucanase (0.017± 0.001 U mL-1). CGP/TCWDE was effective for growth inhibition of Aspergillus fumigatus and Penicillium sp. and the inhibition mechanism seems to involve changes in the cell wall of those microorganisms. Finally, the CGP/TCWDE presented high stability after drying, maintaining enzymatic and biological activity after 200 days of storage at room temperature (25 ºC).
Evaluation of anti atherosclerotic activity of virgin coconut oilRam Sahu
1. The study evaluated the anti-atherosclerotic effects of virgin coconut oil (VCO) in male Wistar rats fed high lipid and high carbohydrate diets to induce atherosclerosis.
2. Rats fed VCO along with normal, high carbohydrate, and high lipid diets had significantly lower body weight, total cholesterol, triglycerides, LDL, and VLDL, but higher HDL compared to control diet groups after 8 weeks.
3. VCO treatment also significantly reduced the atherogenic index and increased percentage of protection against atherosclerosis in all diet groups compared to controls. This suggests VCO has anti-atherosclerotic properties.
Whey protein products and their combination with L-methionine prevent liver f...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
MEMORIAS TRABAJOS LIBRES
Conferencia Científica Anual sobre Síndrome Metabólico 2015
Efecto comparativo de cuatro modelos de dieta con diferente cantidad y tipo de grasa sobre la disfunción del tejido adiposo en pacientes con síndrome metabólico en estado postprandial
PhD María Eugenia Meneses*, PhD Antonio Camargo-García*, PhD Cristina Cruz-Teno*, PhD Yolanda Jiménez-Gómez**, PhD Pablo Pérez-Martínez*, PhD Javier Delgado-Lista*, PhD María del Mar Malagón-Poyato**, PhD Francisco Pérez-Jiménez*, PhD Helen Roche***, PhD José López-Miranda*
* Unidad de Lípidos y Arteriosclerosis, Servicio de Medicina Interna, IMIBIC/Hospital Universitario Reina Sofía/Universidad de Córdoba, Córdoba, España y CIBER Fisiopatología Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, ** Departamento de Biología Celular, Fisiología e Inmunología. IMIBIC, (CIBEROBN).Universidad de Córdoba, España, *** Nutrigenomics Research Group, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Republic of Ireland
This document summarizes a study that investigated the antidiabetic and antioxidant effects of the hydro-alcoholic stem bark extract of Callicarpa arborea Roxb. in diabetic rats. Key findings include:
1) The extract showed significant antioxidant activity in various in vitro assays and was found to be non-toxic up to 2000 mg/kg.
2) In diabetic rats, the extract significantly reduced blood glucose levels, improved glucose tolerance, increased serum insulin levels, and positively affected lipid profiles and liver function markers.
3) Histopathological analysis of pancreas and liver tissues showed protective effects of the extract against diabetes-induced damage.
4) The results suggest that the stem bark
This document discusses the use of herbal immunomodulators in poultry to improve immunity and combat antibiotic resistance. It provides background on immunomodulators and how certain herbs like ashwagandha, neem, tinospora cordifolia, and mint can modulate the immune system through effects on cytokines, immune cells, and phagocytosis. Several studies are summarized that show herbs like ashwagandha and tinospora cordifolia can improve performance parameters and immune responses in broiler chickens and quails. The document concludes that herbs have potential immunomodulatory properties through effects on macrophages, cytokines, and immune cells like NK cells.
This document discusses the use of herbal immunomodulators in poultry to improve immunity and performance. It provides background on how focus on growth has negatively impacted immunity in poultry. Herbal immunomodulators stimulate or suppress immune system components and target macrophages to improve phagocytosis and cytokine production. Several herbs are described that have antimicrobial and immunomodulatory properties, including ashwagandha, neem, tinospora cordifolia, mint, astragalus, and phyllanthus emblica. Studies show herbs like ashwagandha and tinospora cordifolia improve performance parameters and immune responses in poultry. Overall, herbal immunomod
Peas (Pisum sativum) are one of the four most important crops next to soybean, groundnut, and beans. It is a particularly important legume in temperate areas with numerous food (dry seed, vegetable) and feed (seed, fodder) usages.
Global farmed crustacean production has been increasing at much faster rate than major farmed species. It is expected to reach 6.8 million metric tons (MMT) in 2015, of which, 69 percent or 4.7 MMT will be from the marine shrimps (Figures 1A, 1B). In the last 15 years, the production increased six-fold driven more by intensification rather than expansion of the area cultivated.
Isolation, in vitro antidiabetic, antioxidant activity and molecular docking ...iosrjce
The document describes the isolation and characterization of two pentacyclic triterpenoids, friedelin and 3β-friedelinol, from the stem bark of Syzygium alternifolium. The compounds were tested for their antidiabetic and antioxidant activities. Friedelin and 3β-friedelinol showed significant inhibition of α-glucosidase and α-amylase enzymes, decreasing plasma glucose levels and demonstrating antidiabetic effects. They also exhibited antioxidant properties by scavenging free radicals in the DPPH inhibition assay. Molecular docking studies confirmed the binding of friedelin and 3β-friedelinol to α-glucosidase and
1) The document discusses a study on the effects of the ethyl acetate fraction of Syzygium polyanthum (bay leaves) on the histopathological changes in the Langerhans islets of rats with alloxan-induced diabetes.
2) The study found that bay leaves fractions at doses of 125, 250, and 500 mg/kg body weight effectively repaired damage to the Langerhans islets and increased the number of pancreatic beta cells.
3) Bay leaves fractions also repaired the structure of the Langerhans islets after alloxan induction. The study concludes that bay leaves have potential as an alternative treatment for diabetes mellitus.
This study investigated the optimal ratio of methionine to methionine plus cysteine (M:M+C) in the diets of growing pigs under normal conditions and during immune system stimulation (ISS). Thirty-six pigs were fed one of five diets varying in M:M+C ratio and their nitrogen balance was measured before and during two periods of ISS induced by injecting lipopolysaccharide. ISS reduced nitrogen balance and the optimal dietary M:M+C ratio increased during ISS, suggesting methionine is preferentially used during an immune response. The researchers concluded that ISS results in a higher optimal dietary M:M+C ratio in growing pigs.
Systems Nutrition of the Gut-Liver Axis and the Role of the MicrobiomeNorwich Research Park
This document summarizes a presentation on systems nutrition and the role of the gut-liver axis and microbiome. It discusses how the small intestine plays a key role in early pro-inflammatory disturbances by affecting the gut microbiota and their metabolites. The gut microbiota influences the intestinal and systemic metabolome and host metabolic regulation through transcription factors like PPARγ, FXR, and AHR. Beneficial bacteria like Akkermansia muciniphila may lose benefits under certain dietary conditions like heme. Targeting the small intestine and microbiota with foods, bioactives, probiotics or drugs could improve gut and liver health.
feeding pigs trans fat vahmani meadus 2016 cjas-2015-0080Adam Sebzda
1) Sixteen pigs were randomly assigned to diets containing either 5% biohydrogenation product (BHP)-enriched beef fat or control beef fat for 7 weeks.
2) Feeding the enriched fat led to deposition of various BHPs, including trans fatty acids and conjugated linoleic acids, in the pigs' subcutaneous fat tissues.
3) While growth performance and body composition were unaffected, total and HDL cholesterol decreased in pigs fed the enriched fat diet, though LDL cholesterol and triglycerides did not change.
Hydrolysate Characterization Technical Presentation Webinar11 2009Mason Williams
1) The document discusses developing a chemically defined hydrolysate alternative to mitigate risks associated with undefined natural hydrolysates.
2) The approach involves fractionating multiple hydrolysates using RP-HPLC and identifying bioactive fractions that stimulate cell growth and protein production in CHO cells.
3) A new product called EX-CELL CD Hydrolysate Fusion was developed that contains synthetic components identified from bioactive fractions and provides 80-100% of the performance of natural hydrolysates.
More Related Content
Similar to Probiotic effects on fish seminar presentation and literature review (Lina-81405)pptx
Five diets (diets incorporated with folic acid, vitamin B, vitamin C, vitamin/
mineral mix and control) were used to determine their effects on survival, digestive
enzyme activity, protein profiling and growth of striped snakehead Channa striatus
fingerlings. All vitamin diets showed significant difference in enzyme activity like
protease, amylase and lipase, when compared to that of control. The fingerlings fed
with vitamin C diet showed significantly (P<0.05) better performance in terms of
survival rate, weight gain and Specific Growth Rate (SGR) when compared to the rest
of the four diets. The electrophoretic studies revealed that high molecular weight
fractions were added up in muscle tissue of C. striatus fed with vitamin and mineral
supplemented diets.
This document presents a research proposal for evaluating the effect of different stocking densities on growth and physiological function of Ompok bimaculatus (Pabda) reared in a biofloc system. The study will examine growth, survival, water quality, physiological indices, and digestive enzymes of Pabda at stocking densities of 3, 6, and 9 fish/L in biofloc and clear water control systems over 70 days. Expected outcomes include optimizing stocking density for growth and physiology of Pabda in a biofloc system. A literature review supports the research gap on species diversification and effect of stocking density in biofloc systems.
This study investigated the effects of reducing Fsp27 (fat-specific protein 27) levels through antisense oligonucleotides (ASOs) in mouse models of obesity and insulin resistance. Mice fed a high-fat diet or leptin-deficient ob/ob mice were treated with Fsp27 ASOs. Partial reduction of Fsp27 resulted in decreased visceral fat, improved insulin sensitivity and blood glucose control, and changes in genes related to lipid metabolism. The results suggest that reducing FSP27 activity through ASOs could be a potential therapeutic approach for insulin resistance and obesity in patients.
Austin publishing group - Oral kefir grains supplementation improves metaboli...Austin Publishing Group
This study has a novel approach to investigate the effects of oral supplementation of kefir grains on metabolic improvement and the expression of the antioxidant enzymes Glutathione Peroxidase (GPx) and Catalase (CAT) of the liver in malnourished mice.
1) The document evaluates adding high protein DDGS (distiller's dried grain with solubles) feed to vaname shrimp cultivation to overcome AHPND (Acute Hepatopancreatic Necrosis Disease). Water quality parameters like temperature, pH, salinity and dissolved oxygen remained optimal with DDGS addition.
2) Bacterial counts like total vibrio and total bacteria were higher with DDGS, possibly due to the pond's history of AHPND infection. Shrimp in DDGS ponds showed lower survival rates, likely due to an AHPND outbreak from vibrio parahemolyticus bacteria persisting in the pond in a dormant state.
3) While DDGS did not negatively
Development of a Bioactive Food Additive for Controlling of Fungal GrowthIJEAB
Fresh foods have a great importance in human nutrition. However, they are marketed with greatly reduced shelf life mainly due to fungal spoilage. In this work, cell wall degrading enzymes produced by Trichoderma asperellum T00 (TCWDE) were immobilized onto cashew gum polysaccharide (CGP) in order to evaluate the potential use of this material as food additive aiming to increase the shelf life by inhibiting fungal growth. Results from factorial design (32) evidenced that the best conditions for TCWDE immobilization was achieved with 20 min of reaction using 1 mmol L-1 of NaIO4. On these conditions it was observed 91% of retention yield for NAGase (30.1 ± 0.38 U mL-1), 41% for chitinase (0.67 ± 0.05 U mL-1), and 24% for β-1,3-glucanase (0.017± 0.001 U mL-1). CGP/TCWDE was effective for growth inhibition of Aspergillus fumigatus and Penicillium sp. and the inhibition mechanism seems to involve changes in the cell wall of those microorganisms. Finally, the CGP/TCWDE presented high stability after drying, maintaining enzymatic and biological activity after 200 days of storage at room temperature (25 ºC).
Evaluation of anti atherosclerotic activity of virgin coconut oilRam Sahu
1. The study evaluated the anti-atherosclerotic effects of virgin coconut oil (VCO) in male Wistar rats fed high lipid and high carbohydrate diets to induce atherosclerosis.
2. Rats fed VCO along with normal, high carbohydrate, and high lipid diets had significantly lower body weight, total cholesterol, triglycerides, LDL, and VLDL, but higher HDL compared to control diet groups after 8 weeks.
3. VCO treatment also significantly reduced the atherogenic index and increased percentage of protection against atherosclerosis in all diet groups compared to controls. This suggests VCO has anti-atherosclerotic properties.
Whey protein products and their combination with L-methionine prevent liver f...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
MEMORIAS TRABAJOS LIBRES
Conferencia Científica Anual sobre Síndrome Metabólico 2015
Efecto comparativo de cuatro modelos de dieta con diferente cantidad y tipo de grasa sobre la disfunción del tejido adiposo en pacientes con síndrome metabólico en estado postprandial
PhD María Eugenia Meneses*, PhD Antonio Camargo-García*, PhD Cristina Cruz-Teno*, PhD Yolanda Jiménez-Gómez**, PhD Pablo Pérez-Martínez*, PhD Javier Delgado-Lista*, PhD María del Mar Malagón-Poyato**, PhD Francisco Pérez-Jiménez*, PhD Helen Roche***, PhD José López-Miranda*
* Unidad de Lípidos y Arteriosclerosis, Servicio de Medicina Interna, IMIBIC/Hospital Universitario Reina Sofía/Universidad de Córdoba, Córdoba, España y CIBER Fisiopatología Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, ** Departamento de Biología Celular, Fisiología e Inmunología. IMIBIC, (CIBEROBN).Universidad de Córdoba, España, *** Nutrigenomics Research Group, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Republic of Ireland
This document summarizes a study that investigated the antidiabetic and antioxidant effects of the hydro-alcoholic stem bark extract of Callicarpa arborea Roxb. in diabetic rats. Key findings include:
1) The extract showed significant antioxidant activity in various in vitro assays and was found to be non-toxic up to 2000 mg/kg.
2) In diabetic rats, the extract significantly reduced blood glucose levels, improved glucose tolerance, increased serum insulin levels, and positively affected lipid profiles and liver function markers.
3) Histopathological analysis of pancreas and liver tissues showed protective effects of the extract against diabetes-induced damage.
4) The results suggest that the stem bark
This document discusses the use of herbal immunomodulators in poultry to improve immunity and combat antibiotic resistance. It provides background on immunomodulators and how certain herbs like ashwagandha, neem, tinospora cordifolia, and mint can modulate the immune system through effects on cytokines, immune cells, and phagocytosis. Several studies are summarized that show herbs like ashwagandha and tinospora cordifolia can improve performance parameters and immune responses in broiler chickens and quails. The document concludes that herbs have potential immunomodulatory properties through effects on macrophages, cytokines, and immune cells like NK cells.
This document discusses the use of herbal immunomodulators in poultry to improve immunity and performance. It provides background on how focus on growth has negatively impacted immunity in poultry. Herbal immunomodulators stimulate or suppress immune system components and target macrophages to improve phagocytosis and cytokine production. Several herbs are described that have antimicrobial and immunomodulatory properties, including ashwagandha, neem, tinospora cordifolia, mint, astragalus, and phyllanthus emblica. Studies show herbs like ashwagandha and tinospora cordifolia improve performance parameters and immune responses in poultry. Overall, herbal immunomod
Peas (Pisum sativum) are one of the four most important crops next to soybean, groundnut, and beans. It is a particularly important legume in temperate areas with numerous food (dry seed, vegetable) and feed (seed, fodder) usages.
Global farmed crustacean production has been increasing at much faster rate than major farmed species. It is expected to reach 6.8 million metric tons (MMT) in 2015, of which, 69 percent or 4.7 MMT will be from the marine shrimps (Figures 1A, 1B). In the last 15 years, the production increased six-fold driven more by intensification rather than expansion of the area cultivated.
Isolation, in vitro antidiabetic, antioxidant activity and molecular docking ...iosrjce
The document describes the isolation and characterization of two pentacyclic triterpenoids, friedelin and 3β-friedelinol, from the stem bark of Syzygium alternifolium. The compounds were tested for their antidiabetic and antioxidant activities. Friedelin and 3β-friedelinol showed significant inhibition of α-glucosidase and α-amylase enzymes, decreasing plasma glucose levels and demonstrating antidiabetic effects. They also exhibited antioxidant properties by scavenging free radicals in the DPPH inhibition assay. Molecular docking studies confirmed the binding of friedelin and 3β-friedelinol to α-glucosidase and
1) The document discusses a study on the effects of the ethyl acetate fraction of Syzygium polyanthum (bay leaves) on the histopathological changes in the Langerhans islets of rats with alloxan-induced diabetes.
2) The study found that bay leaves fractions at doses of 125, 250, and 500 mg/kg body weight effectively repaired damage to the Langerhans islets and increased the number of pancreatic beta cells.
3) Bay leaves fractions also repaired the structure of the Langerhans islets after alloxan induction. The study concludes that bay leaves have potential as an alternative treatment for diabetes mellitus.
This study investigated the optimal ratio of methionine to methionine plus cysteine (M:M+C) in the diets of growing pigs under normal conditions and during immune system stimulation (ISS). Thirty-six pigs were fed one of five diets varying in M:M+C ratio and their nitrogen balance was measured before and during two periods of ISS induced by injecting lipopolysaccharide. ISS reduced nitrogen balance and the optimal dietary M:M+C ratio increased during ISS, suggesting methionine is preferentially used during an immune response. The researchers concluded that ISS results in a higher optimal dietary M:M+C ratio in growing pigs.
Systems Nutrition of the Gut-Liver Axis and the Role of the MicrobiomeNorwich Research Park
This document summarizes a presentation on systems nutrition and the role of the gut-liver axis and microbiome. It discusses how the small intestine plays a key role in early pro-inflammatory disturbances by affecting the gut microbiota and their metabolites. The gut microbiota influences the intestinal and systemic metabolome and host metabolic regulation through transcription factors like PPARγ, FXR, and AHR. Beneficial bacteria like Akkermansia muciniphila may lose benefits under certain dietary conditions like heme. Targeting the small intestine and microbiota with foods, bioactives, probiotics or drugs could improve gut and liver health.
feeding pigs trans fat vahmani meadus 2016 cjas-2015-0080Adam Sebzda
1) Sixteen pigs were randomly assigned to diets containing either 5% biohydrogenation product (BHP)-enriched beef fat or control beef fat for 7 weeks.
2) Feeding the enriched fat led to deposition of various BHPs, including trans fatty acids and conjugated linoleic acids, in the pigs' subcutaneous fat tissues.
3) While growth performance and body composition were unaffected, total and HDL cholesterol decreased in pigs fed the enriched fat diet, though LDL cholesterol and triglycerides did not change.
Hydrolysate Characterization Technical Presentation Webinar11 2009Mason Williams
1) The document discusses developing a chemically defined hydrolysate alternative to mitigate risks associated with undefined natural hydrolysates.
2) The approach involves fractionating multiple hydrolysates using RP-HPLC and identifying bioactive fractions that stimulate cell growth and protein production in CHO cells.
3) A new product called EX-CELL CD Hydrolysate Fusion was developed that contains synthetic components identified from bioactive fractions and provides 80-100% of the performance of natural hydrolysates.
Similar to Probiotic effects on fish seminar presentation and literature review (Lina-81405)pptx (20)
Probiotic effects on fish seminar presentation and literature review (Lina-81405)pptx
1. Jannatul Ferdush
Exam Roll: 81405
MS session: 2020-2021
Reg. No.: 2016-814-260
Fisheries management Branch
Department of Fisheries
University of Dhaka
Aquaculture and Fisheries 6 (2021) 283–288
Contents lists available at ScienceDirect
Aquaculture and Fisheries
journal homepage: www.keaipublishing.com/en/journals/aquaculture-and-fisheries
Effects of dietary supplementation of Lactobacillus plantarum and
Bacillus subtilis on growth performance, survival, immune response,
antioxidant capacity and digestive enzyme activity in olive flounder
(Paralichthys olivaceus)
Yangzhen Li , Yingming Yang , Limin Song , Jialin Wang , Yuanri Hu , Qian Yang , Peng Cheng , Jianhai Li
Impact factor: 3.69 (2022)
12/10/2022 1
2. 1. Introduction
2. Materials and Methods
3. Results and Discussion
4. Conclusion
Literature
Review
Presentation Outline
12/10/2022 2
4. In aquaculture, Growth and disease resistance are the main parameters of
commercial importance.
Probiotics is a long lasting, less costly and less time consuming solution and
widly used in aquaculture due to their beneficial impacts on,
growth performance and health status
enhance innate immune response
suppress pathogenic microorganisms
L. plantarum and B. subtilis can
improve growth performance,
strengthen immune responses,
modulate activities of antioxidant
and digestive enzymes in aquatic
species (Akhter et al., 2015)
Olive flounder (Paralichthys
olivaceus) is a commercially
important marine flatfish
species in China, South
Korea and Japan.
12/10/2022 4
5. 12/10/2022
The overall objective of the study Research gap
Little information is available on the
beneficial effects of L. plantarum and
B. subtilis during the whole growth
period (from juvenile to commercial
size) under commercial rearing
environment.
To find out the information about
probiotics administration strategy for
improving quality and productivity in
olive flounder.
The specific objectives of the study
To evaluate the growth performance and survival
To investigate the immunological indices, antioxidant ability
To evaluate the digestive enzyme activity
1
2
3
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5
8. 2.1 Diet preparation
8
Diet
Basal diet without
probiotics
Experimental diet
supplemented with
Lactobacillus plantarum
and Bacillus subtilis
Composition Percentage(%)
Crude protein 45.0
Crude fiver 10.0
Crude fat 3.0
Crude ash 16.0
Calcium 4.0
Lysine 2.5
Total phosphorus 1.5-3.0
Table-1 The proximate composition of basal diet on
dry matter basis provided by manufacturer.
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9. 9
Collection of Olive flounder juveniles( approximately four months old)
Fish were fed two times daily
Randomly distributed into three groups and each group consists of three
replicates
Each tank was supplied with filtered and sterilized sea water
Water temperature was controlled at 10–24 ⸰C, the salinity at 28–30ppt,
the dissolved oxygen at 6–8 mg/L, water exchange rate at 500% per day.
2.2 Fish grow-out maintenance
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10. According to, (Crane et al, 2020)
Weight gain rate (WGR):
𝑾𝟏−𝑾𝟎
𝑾𝟎
× 100%
Specific growth rate(SGR):
( 𝒆
𝒍𝒏 𝑾𝟏
−𝒍𝒏 𝑾𝟎
𝑻
−𝟏)
× 𝟏𝟎𝟎%
Survival rate:
𝑵𝟏
𝑵𝟎
×100%
Where,
W0 is the initial body weight
W1 is the harvest body weight
N1 is fish number at harvest of each tank
N0 is fish number at stocking of each tank
T is days of grow-out period
2.3 Growth performance and Survival rate
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11. 2.4 Sample collection procedure
Three starved fish were randomly selected after anesthetized
Approximately 1 mL of whole blood samples were drawn from
the caudal vein using syringes
After clotting the sample at room temperature, Serum was
separated by centrifugation (1500 × g, 10 min, 4 ◦C) and stored
at -80 ◦C until use
Fresh liver, stomach and middle intestine tissue samples were
collected and then stored at -80 ◦C
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12. 2.5 The assay of immune ,antioxidant and digestive parameter
Immune
parameter
• Lysozyme (LYS), Acid phosphatase (ACP),Immunoglobulin M (IgM),
Complement protein 3 (C3) and Complement protein 4 (C4)
Antioxidant
parameter
• Catalase (CAT), Superoxide dismutase (SOD), Glutathione
peroxidase (GSH-Px), Total antioxidant capacity (T-AOC) and
Malondialdehyde (MDA)
Digestive
parameter
• Proteinase, Amylase and Lipase
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13. 13
50 μL diluted samples (1:4) and 100 μL horseradish peroxidase (HRP) conjugated antibody-
enzyme reagent were added
Incubated at 37 ◦C for 60 min and then washed with washing solution for 5 times.
After drying, tetramethyl benzidine (TMB) chromogenic substrate was pipetted and blended
gently
Again after incubation at 37 ◦C for 15 min in darkness, the enzymatic reaction was stopped by
adding 50 μL stopping buffer
Then the absorbance at 450 nm was assayed using a Rayto RT-6100 microplate reader
2.5 Continue…
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14. 2.6 Statistical analysis
All statistical analyses were carried out
using SPSS 25.0 software. The results
were presented as mean ± standard
error (SE).
12/10/2022 14
Normality of all the data and the
homogeneity of variances among
different groups were analyzed by
using Shapiro-Wilk test and Levene’s
test respectively.
One-way analysis of variance (ANOVA)
was performed to determine significant
differences in various parameter indices,
where significance level was set as P-
value < 0.05.
16. Table 2 Initial body weight (IBW), harvest body weight
(HBW), weight gain rate (WGR), specific growth rate
(SGR) and survival rate (SR) of olive flounder. Values are
expressed as mean ± SE. Values with different
superscript letters in the same row show significant
differences (P < 0.05).
Index Diet
L. plantarum B. subtilis Control
IBW (g)
HBW (g)
WGR (%)
SGR
SR (%)
18.54 ± 0.71a
370.7 ± 3.77b
1896.06 ± 66.57b
0.99 ± 0.012b
73.89 ± 2.84a
18.22 ± 0.84a
397.50 ± 3.11a
2085.08 ± 64.10a
1.02 ± 0.010a
66.85 ± 3.56ab
18.87 ± 0.89a
353.29 ± 2.76c
1836.72 ±72.85c
0.98 ± 0.014c
61.94 ± 3.68b
3.1. Growth performance parameters and survival
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The growth performance
parameters were significantly
enhanced by dietary
supplementation feed than
control.
B. subtilis supplementation feed
show great superiority for growth
improvement in olive flounder.
17. 3.2. Immunological indices
Index Diet
L. plantarum B. subtilis Control
LYS (U/L) 5.99 ± 0.11a 4.32 ± 0.08b 3.69 ± 0.07c
ACP (U/L)
IgM (μg/mL)
C3 (μg/mL)
C4 (μg/mL)
11.13 ± 0.22a
46.94 ± 0.41a
150.27 ± 5.09a
195.21 ± 4.21a
7.95 ± 0.25b
42.02 ± 0.17b
132.51 ± 3.06b
133.23 ± 0.69b
7.81 ± 0.15b
37.62 ±0.58c
98.43 ±0.87c
123.76±2.3b
Table3 Effects of L. plantarum and B. subtilis
supplementation in diets on immune response. Values are
expressed as mean ± SE (n = 9). Values with different
superscript letters in the same row show significant
differences (P < 0.05).
LYS: lysozyme; ACP: acid phosphatase IgM:
immunoglobulin M; C3: complement protein 3; C4:
complement protein 4.
Serum immunological indices
are significantly higher for L.
plantarum.
LYS is a bacteriolytic enzyme
which is one of the most
important serum factors
because it can activate the
cellular defenses (Harikrishnan
et al, 2010).
12/10/2022 17
18. 3.3. Hepatic antioxidant capacity
L. plantarum B. subtilis Control
SOD (U/g) 1390.7±57.0b 1590.05±158a 1046.73±93b
CAT (U/g) 538.5 ±34.53a 590.22 ± 42.20a 408.45±30.1b
GSH-Px(U)
TAOC(U/)
MDA(U/g)
1.20 ± 0.11a
133.92±11.5a
80.15 ± 2.63b
1.19 ± 0.08a
128.54 ± 15.36a
75.56 ± 2.26b
0.96 ± 0.15a
80.01±16.10b
106.98±5.50a
Antioxidant enzymes can remove
excessive ROS and protect the cells
from lipid peroxidation.
The lower value of MDA the better the
conditions of liver
Supplemented feed had elevated the
activities of SOD,CAT, GSH-Px and
T-AOC and lowered contents of MDA
in the liver.
Table 4 Effects of L. plantarum and B. subtilis supplementation
in diets on liver antioxidant indices. Values are expressed as
mean ± SE (n = 9). Values with different superscript letters in
the same row show significant differences (P < 0.05).
SOD: superoxide dismutase; CAT: catalase, GSH-Px: glutathione
peroxidase, T-AOC: total antioxidant capacity; MDA:
malondialdehyde.
18
19. 3.4.1. protease enzyme activity
Fig. 1. Protease activities in liver, stomach and intestine.
Different superscripts indicate significant differences (P
< 0.05) between treatments in the same viscus. 19
For stomach and intestine
protease activity were
significantly higher in L.
plantarum and B. subtilis than
control.
For liver B. subtilis
supplemented feed was higher
L. plantarum
20. 3.4.2. Lipase activity
Lipase activity in dietary B.
subtilis supplementation group
was substantially (significant in
intestine and stomach, and
insignificant in liver) higher
than L. plantarum group.
Fig 2. Lipase activities in liver, stomach and intestine.
Different superscripts indicate significant differences (P <
0.05) between treatments in the same viscus.
20
21. 3.4.3. Amylase activity
In liver the improvement levels of
both supplemented feed was
negligible.(p>0.05)
In stomach and intestine amylase
activity was significantly higher in
both supplemeted feed than
control.
But L. plantarum activity was not
significant than control.
Fig. 3. Amylase activities in liver, stomach and
intestine. Different superscripts indicate significant
differences (P < 0.05) between treatments in the same
viscus.
21
23. Separate supplementation of L. plantarum and B. subtilis in diet is more
beneficial for disease resistance (high survival) and growth performance
respectively.
Feed supplemented with B. subtilis and L. plantarum had a beneficiary
effects on innate immune indices, antioxidant and digestive activities.
The beneficiary compounded supplementation of L. plantarum and B.
subtilis in feed can be a great interest in fish farming industries, feed and
additive manufacturers and even aquaculture researchers.
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24. 12/10/2022 24
Thesis title
Effects of probiotics on digestive enzymes,
antioxidant enzymes and immune defense in Gulsha
Tengra (Mystus cavasius)
25. Received: 5 March 2020 | Revised: 26 October 2020 | Accepted: 29 October 2020
DOI: 10.1111/are.15009
Growth performances, water quality, digestive enzyme and antioxidant enzymes in
liver spleen and kidney were improved.
16S rRNA indicated that, modulate gut microbial communities and significantly
increase functions relating to nitrogen metabolism, phosphorylation and proteinases.
O R I G I N A L A R T I C L E
Effects of Clostridium butyricum on growth, digestive enzyme activity, antioxidant
capacity and gut microbiota in farmed tilapia (Oreochromis niloticus)
Mingqing Zhang1 | Bo Dong2 | Xingxing Lai1 | Zhuang Chen2 | Liping Hou1 | Rui Shu3 | Yanhua Huang4 | Hu Shu1
Key Findings
12/10/2022 25
26. Probiotics and Antimicrobial proteins (2021) 13:1723–1733
https://doi.org/10.1007/s12602-021-09787-3
The Effect of Lactococcus lactis subsp. lactis PTCC 1403 on the Growth
Performance, Digestive Enzymes Activity, Antioxidative Status, Immune
Response, and Disease Resistance of Rainbow Trout (Oncorhynchus
mykiss)
Sakineh Yeganeh1 · Milad Adel2 · Ahmad Nosratimovafagh1 · Mahmoud A. O. Dawood3
Dietary Lactococcus lactis subsp. lactis PTCC 1403 enhanced the feed
efficiency, digestive enzyme activity, antioxidative status, and immunity of
rainbow trout.
Increased resistance against infection.
Key Findings
Published online: 1 June 2018
# Springer Science+Business Media, LLC, part of Springer Nature 2018, corrected publication June/2018
12/10/2022 26
27. Aquaculture Nutrition
Volume 2022, Article ID 8968494, 17 pages
https://doi.org/10.1155/2022/8968494
Hindawi
Research Article
Effects of Single and Mixture Probiotic Supplements on Growth,Digestive Activity,
Antioxidative Status, Immune and Growth- Related Genes, and Stress Response of
Juvenile Red Sea Bream (Pagrus Major)
Ronick Spenly Shadrack ,1,2 Ishikawa Manabu,2 Shunsuke Koshio,2 Saichiro Yokoyama,2
Yukun Zhang,1 Kumbukani Mzengereza ,1 Mohammed Fouad El Basuini ,3,4
and Mahmoud A. O. Dawood 5,6
Mixed probiotic bacteria strain containing commercial strain has significant potential
to improve growth performance, feed utilization, and immune response in red sea
bream.
Key Findings
12/10/2022 27
28. Probiotics and Antimicrobial Proteins (2019) 11:545–558
https://doi.org/10.1007/s12602-018-9409-8
Dietary Supplementation of Probiotic Bacillus subtilis Affects
Antioxidant Defenses and Immune Response in Grass Carp Under
Aeromonas hydrophila Challenge
Ying Tang1,2 & Lei Han1,3 & Xiaoxuan Chen1,2 & Mengqi Xie1,2 & Weiguang Kong1,4 & Zhixin Wu1,2
Published online: 13 April 2018
# Springer Science+Business Media, LLC, part of Springer Nature 2018
Key Findings
Bacillus subtilis CH9 strain, isolated and identified used as a probiotic to
protect grass carp against pathogenic strain of Aeromonas hydrophila, and
improved fish antioxidant defences and immune response.
12/10/2022 28
29. Aquaculture 294 (2009) 306–313
Contents lists available at ScienceDirect
Aquaculture
journal homepage: www.elsevier.com/ locate/aqua-online
Effect of dietary probiotic Pediococcus acidilactici on antioxidant
defences and
oxidative stress status of shrimp Litopenaeus stylirostris
Mathieu Castex ⁎, Pierrette Lemaire, Nelly Wabete, Liet Chim
Key Findings
Probiotic supplementation in shrimps' diet is an effective way to reduce the
susceptibility of shrimps to bacterial pathogens.
Oxidative stress reduced in the shrimps fed with the probiotic diet.
Lower the load of Vibrio in the shrimps' haemolymph and higher survival rates.
12/10/2022 29
30. Aquaculture 16 (2020) 100237
Contents lists available at ScienceDirect
Aquaculture
journal homepage: www.elsevier.com/ locate/aqrep
Mst Rubia Banua,*, Shammee Aktera, Md Reazul Islamb, Md Nurunnabi Mondola, Md Amzad Hossainc
Key Findings
The growth performance of gulsa tengra in terms of final body weight, weight
gain, percent weight gain, and specific growth rate was significantly higher.
Higher fish survivability, proximate composition.
FCR(food conversion ratio) was lowered in supplemented feed.
Probiotic yeast enhanced growth performance and disease
resistance in freshwater catfish gulsa tengra, Mystus cavasius
12/10/2022 30
31. Journal of Oceanology and Limnology
Vol. 35 No. 1, P. 89-97, 2017
http://dx.doi.org/10.1007/s00343-016-5130-1
Effects of small peptides, probiotics, prebiotics, and synbiotics on
growth performance, digestive enzymes, and oxidative stress in
orange-spotted grouper
Epinephelus coioides ,juveniles reared in artificial seawater
WANG Tao (王涛), CHENG Yongzhou (成永洲), CHEN Xiaoyan (陈晓艳), LIU Zhaopu (刘兆普), LONG Xiaohua (隆
小华) **
Key Findings
Digestive enzyme activities significantly enhanced by the small peptide
supplement.
Significantly improved growth rates of grouper under artificial seawater
conditions.
Dietary supplements did not improve antioxidant capacity under low-salinity
stress conditions.
12/10/2022 31
32. Received: 3 September 2018 | Revised: 28 January 2019 | Accepted: 9 February 2019
DOI: 10.1111/anu.12904
ORIGINAL ARTICLE
Dietary supplementation effects of Pediococcus acidilactici as probiotic
on growth performance, digestive enzyme activities and immunity
response in zebrafish (Danio rerio)
Mojtaba Mohammadi Arani1,2 | Amir Parviz Salati1 | Omid Safari3 |Saeed Keyvanshokooh1
Key Findings
The inclusion of P. acidilactici in the diet is an effective way not only to reduce
both the production period and costs, but also to improve fish health.
It is suggest that 4 × 106 cfu of the probiotic P. acidilactici in each gram of the
zebrafish diet improved growth performance, digestive enzyme and immune
response.
12/10/2022 32
33. Probiotics and Antimicrobial Proteins (2019) 11:938–945
https://doi.org/10.1007/s12602-018-9421-z
Effects of Dietary Clostridium butyricum on the Growth,Digestive Enzyme
Activity, Antioxidant Capacity, and Resistance to Nitrite Stress of Penaeus
monodon
Yafei Duan1 & Jiasong Zhang1 & Jianhua Huang1,2 & Shigui Jiang1
Published online: 1 June 2018
# Springer Science+Business Media, LLC, part of Springer Nature 2018, corrected publication June/2018
Key Findings
Clostridium butyricum is a good probiotic and its optimum dosage is 1.0% (w/w) for
shrimp aquaculture.
Dietary supplementation with C. butyricum improved the growth and intestine
digestive enzyme activity, intestine antioxidant capacity and nitrite stress resistance
capacity in Penaeus monodon, with no effects on the shrimp body composition.
12/10/2022 33
34. Aquaculture 531 (2021) 735852
Contents lists available at ScienceDirect
Aquaculture
journal homepage: www.elsevier.com/ locate/aqua-online
Key Findings
Lactic acid bacteria supplementation enhanced the liver and intestine antioxidant
status and significant improvement in the digestive enzyme activity.
Increase the generation of probiotic bacteria and decrease the abundance of aquatic
pathogens.
The positive effects of single or conjoint administration of lactic acid
bacteria on Channa argus: Digestive enzyme activity, antioxidant capacity,
intestinal microbiota and morphology
Yidi Konga, Min Lia,c,d,e, Guosheng Chub, Hongjian Liub, Xiaofeng Shana,c,d,e,Guiqin Wanga,c,d,e, Guanghong Hanf,
12/10/2022 34