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Vincent Denef
Ecology and Evolutionary Biology, University of Michigan
Water @ Michigan
January 21, 2016
The difference a species makes:
impact of invasive species on freshwater microbial
communities
@VJDenef
vdenef@umich.edu
The difference a species makes
Texasinvasives.org
Invasion of the Dreissenids
[Credit: Nalepa, GLERL
Invasion of the Dreissenids
The difference a species makes
[Higgins, Ecol Mono, 2010]
Bacteria 0 ? ? ?
Invasion of the Dreissenids
The difference a species makes
Biota
Phytoplankton
Micro/nanozooplankton
Phototrophic
bacteria
Ecosystem
impacts
Chemotrophic
Bacteria (benthic-pelagic)
Microbial – higher
food web connectivity
Geochemical cycling
(e.g. CO2 efflux)
[Buffam et al, GCB, 2011]
July ‘99
July ‘10
[Credit: Vanderploeg, GLERL]
Chl a concentration
Impacts on bacterial communities
[Credit: Vanderploeg, GLERL]
Harmful algal blooms
(e.g., Microcystis)
The difference a species makes
Source water: Lake MI M45, 100 micron pre-filtered
Quagga mussels: Lake MI M45, cleaned and acclimated in lab for 12 hrs
20L
3 hrs
4 °C
20L
20L
20L
X 3
X 4
X 3
X 4
16S rRNA V4
sequencing
Tom Johengen Hank Vaderploeg
Experimental design
Feeding experiments
* = statistically significant difference (LEfSe, p < 0.05)
Impacts on bacterial communities: feeding experiment
Feeding experiments
Impacts on bacterial communities: field observations
Field observations
Impacts on bacterial communities: field observations
Field observations
Acknowledgments
NOAA GLERL
Ashley Baldridge
Joann Cavaletto
Jim Liebig
Ed Rutherford
Henry Vanderploeg
Crew of the R/V Laurentian
University of Michigan
Michelle Berry
Ashley Burtner
Edna Chiang *
Seana Florida *
Masanori Fujimoto ***
Thomas Johengen
Ann McCarthy
Ryan Keeling *
Morgan Meade *
Justin Millar
Tom Nalepa
Danna Palladino
Marian Schmidt **
Amelia Waters *
Amadeus Twu *
Support

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The Difference a Species Makes on Freshwater Microbial Communities

  • 1. Vincent Denef Ecology and Evolutionary Biology, University of Michigan Water @ Michigan January 21, 2016 The difference a species makes: impact of invasive species on freshwater microbial communities @VJDenef vdenef@umich.edu
  • 2. The difference a species makes Texasinvasives.org Invasion of the Dreissenids
  • 3. [Credit: Nalepa, GLERL Invasion of the Dreissenids The difference a species makes
  • 4. [Higgins, Ecol Mono, 2010] Bacteria 0 ? ? ? Invasion of the Dreissenids The difference a species makes
  • 5. Biota Phytoplankton Micro/nanozooplankton Phototrophic bacteria Ecosystem impacts Chemotrophic Bacteria (benthic-pelagic) Microbial – higher food web connectivity Geochemical cycling (e.g. CO2 efflux) [Buffam et al, GCB, 2011] July ‘99 July ‘10 [Credit: Vanderploeg, GLERL] Chl a concentration Impacts on bacterial communities [Credit: Vanderploeg, GLERL] Harmful algal blooms (e.g., Microcystis) The difference a species makes
  • 6. Source water: Lake MI M45, 100 micron pre-filtered Quagga mussels: Lake MI M45, cleaned and acclimated in lab for 12 hrs 20L 3 hrs 4 °C 20L 20L 20L X 3 X 4 X 3 X 4 16S rRNA V4 sequencing Tom Johengen Hank Vaderploeg Experimental design Feeding experiments
  • 7. * = statistically significant difference (LEfSe, p < 0.05) Impacts on bacterial communities: feeding experiment Feeding experiments
  • 8. Impacts on bacterial communities: field observations Field observations
  • 9. Impacts on bacterial communities: field observations Field observations
  • 10. Acknowledgments NOAA GLERL Ashley Baldridge Joann Cavaletto Jim Liebig Ed Rutherford Henry Vanderploeg Crew of the R/V Laurentian University of Michigan Michelle Berry Ashley Burtner Edna Chiang * Seana Florida * Masanori Fujimoto *** Thomas Johengen Ann McCarthy Ryan Keeling * Morgan Meade * Justin Millar Tom Nalepa Danna Palladino Marian Schmidt ** Amelia Waters * Amadeus Twu * Support