Urinary system infections are the most common community acquired infections. The main reason of that is Escherichia coli (E. coli). People are referred to a doctor cause of urine infections every year around 8.3 millions in the world. On the other hand, active oxygen forms are formed during normal processes of body using oxygen in human metabolism. When these forms are not prevented, they would cause structural degradation of DNA, proteins, carbohydrates and lipids. Thus, they cause many degenerative diseases by disrupting the structure and function of the cell membrane. Synthetic antioxidants are effective and cheap for a long period of time, but their antioxidants have decreased their attractiveness when negative effects on human health are revealed. The World Health Organization (WHO) has reported that approximately 20,000 plants are used for medical treatments. For this purpose, it was aimed to investigate the antimicrobial and antioxidant effects of Lamium galeobdolon L., Alpinia officinarum L. and Peganum harmala L. plants which are used in public due to effects haven’t clarified yet.
The plants were prepared by extracting in methanol with Soxhlet extraction method. DPPH (2,2-diphenyl-1-picrylhydrazyl), which shows free radical properties for antioxidant activity determination, was dissolved in methanol and they were used at different concentrations. Free radical scavenger fractions of the extracts were calculated using spectrophotometer and the concentration / inhibition percentages were plotted. For the determination of antimicrobial activity, plant extracts were evaluated by using E. coli bacteria. Therefore cefotaxime antibiotic was used as a positive control and methanol was used as a negative control. Disc diffusion diameters were calculated according to the antibiotics of each plant extraction and compared with each control group.
In this respect, we have demonstrated free radical scavenging activities and antimicrobial activities by using Alpinia officinarum L., Peganum harmala L. and Lamium galeobdolon L. plants, which have been used by the public but whose effects haven’t enough researches yet. Finally by chosen these plants, we have shown that Alpinia officinarum L. can be used as a new natural resource in food and pharmaceutical fields due to its strong antimicrobial and antioxidant properties.
#SciChallenge2017
PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF VARIOUS SOLVENT EXTRAC...IJSIT Editor
The leaves of the plant Annonareticulata were collected and extracted using different ranges of polar
organic solvents like low (Ethyl acetate), medium (Butanol) and high (Methanol). Qualitative analysis and
antimicrobial activity was investigated. The phytochemical screening of the leaf extract revealed that the
presence of alkaloids, tannins, steroids, terpenoids and coumarins. The Ethyl acetate and Methanol extracts
showed better antibacterial activity, the significant inhibitory effect against Escherichia coli, Pseudomonas
putida and Lactobacillus acidophilus, and thus displayed highest inhibitory zone of 19.5mm, 19mm and 19mm
when compared to Butanol. FT-IR spectroscopic analysis of the Ethyl acetate, Butanol and Methanol extract of
A.reticulata revealed the presence of -CH, -OH, CH-OH and –NH2 bond stretching. The clinical isolates were
collected from patients suffered from different microbial infections. The antibacterial and antifungal activity
was determined by using leaf extracts.
Role of Chemists in Drug Design and DiscoveryDrSSreenivasa
Here different sources of drugs and their important components identifications are discussed and how chemists are involved in the development of Drugs is discussed here
Studies on the Antioxidant Properties of Various extracts of Hippophae rhamnoideIJEAB
Sea Buckthorn (Hippophae rhamnoides) a spiny shrub native to Ladakh Region of Jammu and Kashmir, have been found to posses so many medicinal properties from times immoral. From this point of view the antioxidant property of the plant fruit extracts have been analysed by DPPH method. Various plant extracts viz, fruit, leaf and root have been analysed for the antioxidant power determination in which fruit extracts showed highest free radical scavenging activity followed by leaf and root extracts. Among the solvents which have been used, more polar solvents showed highest antioxidant activity than the less polar solvent extracts. The IC50 value of various plant extracts as determined have been found to be 40 for DCM extract of fruit, 38 for Methanolic extract of fruit and 30 for the water extract of fruit. Similarly the leaf extracts posses IC50 value as 51, 47 and 37 respectively for DCM, Methanol and Water extracts. The IC50 values of various root extracts have been found to be 53, 50 and 48 respectively for DCM, Methanol and Water.
IJERA (International journal of Engineering Research and Applications) is International online, ... peer reviewed journal. For more detail or submit your article, please visit www.ijera.com
PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF VARIOUS SOLVENT EXTRAC...IJSIT Editor
The leaves of the plant Annonareticulata were collected and extracted using different ranges of polar
organic solvents like low (Ethyl acetate), medium (Butanol) and high (Methanol). Qualitative analysis and
antimicrobial activity was investigated. The phytochemical screening of the leaf extract revealed that the
presence of alkaloids, tannins, steroids, terpenoids and coumarins. The Ethyl acetate and Methanol extracts
showed better antibacterial activity, the significant inhibitory effect against Escherichia coli, Pseudomonas
putida and Lactobacillus acidophilus, and thus displayed highest inhibitory zone of 19.5mm, 19mm and 19mm
when compared to Butanol. FT-IR spectroscopic analysis of the Ethyl acetate, Butanol and Methanol extract of
A.reticulata revealed the presence of -CH, -OH, CH-OH and –NH2 bond stretching. The clinical isolates were
collected from patients suffered from different microbial infections. The antibacterial and antifungal activity
was determined by using leaf extracts.
Role of Chemists in Drug Design and DiscoveryDrSSreenivasa
Here different sources of drugs and their important components identifications are discussed and how chemists are involved in the development of Drugs is discussed here
Studies on the Antioxidant Properties of Various extracts of Hippophae rhamnoideIJEAB
Sea Buckthorn (Hippophae rhamnoides) a spiny shrub native to Ladakh Region of Jammu and Kashmir, have been found to posses so many medicinal properties from times immoral. From this point of view the antioxidant property of the plant fruit extracts have been analysed by DPPH method. Various plant extracts viz, fruit, leaf and root have been analysed for the antioxidant power determination in which fruit extracts showed highest free radical scavenging activity followed by leaf and root extracts. Among the solvents which have been used, more polar solvents showed highest antioxidant activity than the less polar solvent extracts. The IC50 value of various plant extracts as determined have been found to be 40 for DCM extract of fruit, 38 for Methanolic extract of fruit and 30 for the water extract of fruit. Similarly the leaf extracts posses IC50 value as 51, 47 and 37 respectively for DCM, Methanol and Water extracts. The IC50 values of various root extracts have been found to be 53, 50 and 48 respectively for DCM, Methanol and Water.
IJERA (International journal of Engineering Research and Applications) is International online, ... peer reviewed journal. For more detail or submit your article, please visit www.ijera.com
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Mormodica charantia Linn. A Potential Antibiotic and Anti-Fungal Druginventionjournals
Resistant or multi resistant strains are continuously appearing in the treatment of ailments and this necessitates the synthesis of new drugs especially from naturally occurring plants. Momordica charantia is a very common indigenous plant of tropical and sub-tropical regions of the world. Phytochemical screening, antimicrobial analysis were conducted using recommended procedures. Ethanol and water were used for the extraction and Ultraviolet Visible and Infrared Spectroscope were used to identify the functional groups. Alkaloids, flavonoids, saponins, tannins, anthraquinones were all present in both the ethanolic and aqueous extracts, terpenoids was only present in aqueous extract while cardiac glycosides was not present in both extracts. Concentration (200mg/ml) revealed the highest clear zone of inhibition in both aqueous and ethanol extracts. The zone of inhibition increases with increase in concentration of sample. The ethanol extract shows the higher clear zone of inhibition when compared to aqueous extract with a diameter of zones of inhibition of 28, 26, 24, 26, 26 and 24mm for Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginesa, Salmonella typhi and Klebsiella pneumoniae respectively. The aqueous extract has no effect at concentration (6.25mg/ml) because no clear zone of inhibition was measured. The antimicrobial activity for aqueous extract was lower than and not as effective as ethanol extract because ethanol is a phenolic compound and non-polar solvent when compared with water that is a polar solvent. Infrared spectra showed OH stretch due to phenol was observed at 3702 cm-1 , - C - H stretch (alkane) at 2972cm-1 , C - H bending vibration (alkane) at 1381cm-1 , - C - N and – C = N at 2077cm-1 , NH stretching at 3375cm-1 . The results indicate that it contained a phenolic, amine and amide substitute. Therapeutic drugs with antibiotic and antifungal activities can be synthesized from Mormodica charantia.
Asparaginase is an important enzyme in Medicine & food industry. It catalyzes Asparagine to aspartate and Ammonia. The purpose of using asparaginase in foods is to reduce the levels of acrylamide that form in certain carbohydrate-rich foods during cooking.The rationale behind asparaginase is that it takes advantage of the fact that acute lymphoblastic leukemia cells and some other suspected tumor cells are unable to synthesize the non-essential amino acid asparagine, whereas normal cells are able to make their own asparagine.
ABSTRACT- The present study was planned to study the antimicrobial activity of different plant extract against selected microorganisms. The plants used in the present study were Ocimum sanctum (Tulsi), Withania somnifera (Ashwgandha), Santalum album (Chandan), Aloe vera (Aloe barbadensis), and shatavari (Asparagus racemosus). The extract from the leaves of these plants (are) used in malaria, bronchitis, gastric disorders, cough, cold etc. To test efficiency of some common plants extract against E. coli, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus. Contrary to the synthetic drugs, antimicrobials of plant origin are not associated with many side effects and have an enormous therapeutic potential to heal many infectious diseases. The present investigation is therefore, undertaken to test the efficiency of some of the common plant extracts against some plants and human pathogens, i.e. E. coli and S. aureus. In this project work, we studied the different parts of medicinal plants of Latur, Osmanabad region used for curing different type of diseases specially skin diseases. Some plants have active components which show antimicrobial activity. These Herbal plants are beneficial to human being in therapeutic practice. Skin diseases are difficult conditions to live with, to save the very least. Though some skin diseases may cause minimal discomfort, the visual effects of the conditions can cause significant self esteem and confidence issues. The majority of skin diseases cause scarring or disfigurement. Skin diseases run the gambit from barely noticeable to fatal.
Key-words- Medicinal plants, Antimicrobial activity, Antifungal activity
Extraction of Secondary Metabolites from Roots of Acanthus Ilicifolius L and ...inventionjournals
The root extracts of Acanthus ilicifolius L finds a prominent place in folk medicine. In this study, we
extracted alkaloid, flavonoid, tannin and total phenols in benzene, ethyl acetate, acetone, methanol and
ethanol, their antibacterial activity and antioxidant activity was evaluated. The antioxidant activity is executed
by FRAP assay and agar well diffusion method is done to study the antibacterial activity against Enterobacter
aerogenes, Enterobacter cloacae, Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Streptococcus
pyogenes. The antibacterial activity of all the extracts was compared with standard antibiotic gentamicin.
The Minimum Inhibitory Concentration [MIC] was determined by serial dilution method. Alkaloids are rich in
acetone and Flavonoids are high in methanol extracts. The acetone extract showed higher antioxidant activity,
while benzene extract was identified to contain lower antioxidant activity. The extent of inhibition by the root
extracts diverge between the solvents used, among them ethanol extracts exhibited higher level of inhibition
against the gram positive test cultures compared to gram negative test cultures employed. Whereas, the acetone
extracts efficacy is more on gram negative test cultures than the gram positive cultures. The MIC was found to
be between 1mg/100µl to 5mg/100µl. This study gives the source for purification and characterization of
bioactive principles that possess antioxidant and antibacterial action from the root of Acanthus ilicifolius.
ANTIBACTERIAL ACTIVITY OF Citrus limonON Acnevulgaris (PIMPLES) IJSIT Editor
Research was carried out on antibacterial activity of Citrus limon on Acnevulgaris (Pimples). Samples
were obtained from individuals having Pimples, by swabbing their faces, backs and chests. Samples were
collected from Amanawa hospital in sokoto, Nigeria using Swab sticks. The sticks were transported to the
Microbiology Laboratory of the Usmanu Danfodiyo University Sokoto. Citrus limon juice was used at different
concentrations of (20%, 40%, 60%, 80% and 100%) on Propionibacteriumacnes, the bacteria that cause
Acnevulgaris (Pimples). The Citrus limon juice was found to be effective at all Concentrations used.
Conventional Cleanser was used as positive control, and it was only found to be effective at higher
concentrations of (60%, 80% and 100%) and was not effective at Lower Concentrations (20% and 40%). The
Minimum Inhibitory Concentration (MIC) of Citrulimon on Propionibacterium acnes was taken and presence
of growth was observed at concentrations of 20%, 40% and 60%, and absence of growth was observed at
80% and 100%. The minimum inhibitory concentration of conventional cleanser indicated the presence of
growth at 20% and 40% and absence of Growth at 60%, 80% and 100%. The Minimum bactericidal
Concentration (MBC) taken on Propionibacteriumacnes using both Citrus limon juice and cleanser all showed
absence of growth at all the concentrations used (20%, 40%, 60%, 80% and 100%). From the research
conducted it was observed that lemon juice have strong anti Acne vulgaris effect morethan the convensional
cleansers used for the treatment of Acne vulgaris.
Mormodica charantia Linn. A Potential Antibiotic and Anti-Fungal Druginventionjournals
Resistant or multi resistant strains are continuously appearing in the treatment of ailments and this necessitates the synthesis of new drugs especially from naturally occurring plants. Momordica charantia is a very common indigenous plant of tropical and sub-tropical regions of the world. Phytochemical screening, antimicrobial analysis were conducted using recommended procedures. Ethanol and water were used for the extraction and Ultraviolet Visible and Infrared Spectroscope were used to identify the functional groups. Alkaloids, flavonoids, saponins, tannins, anthraquinones were all present in both the ethanolic and aqueous extracts, terpenoids was only present in aqueous extract while cardiac glycosides was not present in both extracts. Concentration (200mg/ml) revealed the highest clear zone of inhibition in both aqueous and ethanol extracts. The zone of inhibition increases with increase in concentration of sample. The ethanol extract shows the higher clear zone of inhibition when compared to aqueous extract with a diameter of zones of inhibition of 28, 26, 24, 26, 26 and 24mm for Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginesa, Salmonella typhi and Klebsiella pneumoniae respectively. The aqueous extract has no effect at concentration (6.25mg/ml) because no clear zone of inhibition was measured. The antimicrobial activity for aqueous extract was lower than and not as effective as ethanol extract because ethanol is a phenolic compound and non-polar solvent when compared with water that is a polar solvent. Infrared spectra showed OH stretch due to phenol was observed at 3702 cm-1 , - C - H stretch (alkane) at 2972cm-1 , C - H bending vibration (alkane) at 1381cm-1 , - C - N and – C = N at 2077cm-1 , NH stretching at 3375cm-1 . The results indicate that it contained a phenolic, amine and amide substitute. Therapeutic drugs with antibiotic and antifungal activities can be synthesized from Mormodica charantia
This is an Engg Biotechnology project based on medicinal plant i.e singapore cherry or jamaican cherry tree (scientific name Muntingia calabure ), we did in 2013 in GMIT college Davangere, karanataka, India. i have complete project detail what we did..,
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Mormodica charantia Linn. A Potential Antibiotic and Anti-Fungal Druginventionjournals
Resistant or multi resistant strains are continuously appearing in the treatment of ailments and this necessitates the synthesis of new drugs especially from naturally occurring plants. Momordica charantia is a very common indigenous plant of tropical and sub-tropical regions of the world. Phytochemical screening, antimicrobial analysis were conducted using recommended procedures. Ethanol and water were used for the extraction and Ultraviolet Visible and Infrared Spectroscope were used to identify the functional groups. Alkaloids, flavonoids, saponins, tannins, anthraquinones were all present in both the ethanolic and aqueous extracts, terpenoids was only present in aqueous extract while cardiac glycosides was not present in both extracts. Concentration (200mg/ml) revealed the highest clear zone of inhibition in both aqueous and ethanol extracts. The zone of inhibition increases with increase in concentration of sample. The ethanol extract shows the higher clear zone of inhibition when compared to aqueous extract with a diameter of zones of inhibition of 28, 26, 24, 26, 26 and 24mm for Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginesa, Salmonella typhi and Klebsiella pneumoniae respectively. The aqueous extract has no effect at concentration (6.25mg/ml) because no clear zone of inhibition was measured. The antimicrobial activity for aqueous extract was lower than and not as effective as ethanol extract because ethanol is a phenolic compound and non-polar solvent when compared with water that is a polar solvent. Infrared spectra showed OH stretch due to phenol was observed at 3702 cm-1 , - C - H stretch (alkane) at 2972cm-1 , C - H bending vibration (alkane) at 1381cm-1 , - C - N and – C = N at 2077cm-1 , NH stretching at 3375cm-1 . The results indicate that it contained a phenolic, amine and amide substitute. Therapeutic drugs with antibiotic and antifungal activities can be synthesized from Mormodica charantia.
Asparaginase is an important enzyme in Medicine & food industry. It catalyzes Asparagine to aspartate and Ammonia. The purpose of using asparaginase in foods is to reduce the levels of acrylamide that form in certain carbohydrate-rich foods during cooking.The rationale behind asparaginase is that it takes advantage of the fact that acute lymphoblastic leukemia cells and some other suspected tumor cells are unable to synthesize the non-essential amino acid asparagine, whereas normal cells are able to make their own asparagine.
ABSTRACT- The present study was planned to study the antimicrobial activity of different plant extract against selected microorganisms. The plants used in the present study were Ocimum sanctum (Tulsi), Withania somnifera (Ashwgandha), Santalum album (Chandan), Aloe vera (Aloe barbadensis), and shatavari (Asparagus racemosus). The extract from the leaves of these plants (are) used in malaria, bronchitis, gastric disorders, cough, cold etc. To test efficiency of some common plants extract against E. coli, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus. Contrary to the synthetic drugs, antimicrobials of plant origin are not associated with many side effects and have an enormous therapeutic potential to heal many infectious diseases. The present investigation is therefore, undertaken to test the efficiency of some of the common plant extracts against some plants and human pathogens, i.e. E. coli and S. aureus. In this project work, we studied the different parts of medicinal plants of Latur, Osmanabad region used for curing different type of diseases specially skin diseases. Some plants have active components which show antimicrobial activity. These Herbal plants are beneficial to human being in therapeutic practice. Skin diseases are difficult conditions to live with, to save the very least. Though some skin diseases may cause minimal discomfort, the visual effects of the conditions can cause significant self esteem and confidence issues. The majority of skin diseases cause scarring or disfigurement. Skin diseases run the gambit from barely noticeable to fatal.
Key-words- Medicinal plants, Antimicrobial activity, Antifungal activity
Extraction of Secondary Metabolites from Roots of Acanthus Ilicifolius L and ...inventionjournals
The root extracts of Acanthus ilicifolius L finds a prominent place in folk medicine. In this study, we
extracted alkaloid, flavonoid, tannin and total phenols in benzene, ethyl acetate, acetone, methanol and
ethanol, their antibacterial activity and antioxidant activity was evaluated. The antioxidant activity is executed
by FRAP assay and agar well diffusion method is done to study the antibacterial activity against Enterobacter
aerogenes, Enterobacter cloacae, Escherichia coli, Bacillus subtilis, Staphylococcus aureus and Streptococcus
pyogenes. The antibacterial activity of all the extracts was compared with standard antibiotic gentamicin.
The Minimum Inhibitory Concentration [MIC] was determined by serial dilution method. Alkaloids are rich in
acetone and Flavonoids are high in methanol extracts. The acetone extract showed higher antioxidant activity,
while benzene extract was identified to contain lower antioxidant activity. The extent of inhibition by the root
extracts diverge between the solvents used, among them ethanol extracts exhibited higher level of inhibition
against the gram positive test cultures compared to gram negative test cultures employed. Whereas, the acetone
extracts efficacy is more on gram negative test cultures than the gram positive cultures. The MIC was found to
be between 1mg/100µl to 5mg/100µl. This study gives the source for purification and characterization of
bioactive principles that possess antioxidant and antibacterial action from the root of Acanthus ilicifolius.
ANTIBACTERIAL ACTIVITY OF Citrus limonON Acnevulgaris (PIMPLES) IJSIT Editor
Research was carried out on antibacterial activity of Citrus limon on Acnevulgaris (Pimples). Samples
were obtained from individuals having Pimples, by swabbing their faces, backs and chests. Samples were
collected from Amanawa hospital in sokoto, Nigeria using Swab sticks. The sticks were transported to the
Microbiology Laboratory of the Usmanu Danfodiyo University Sokoto. Citrus limon juice was used at different
concentrations of (20%, 40%, 60%, 80% and 100%) on Propionibacteriumacnes, the bacteria that cause
Acnevulgaris (Pimples). The Citrus limon juice was found to be effective at all Concentrations used.
Conventional Cleanser was used as positive control, and it was only found to be effective at higher
concentrations of (60%, 80% and 100%) and was not effective at Lower Concentrations (20% and 40%). The
Minimum Inhibitory Concentration (MIC) of Citrulimon on Propionibacterium acnes was taken and presence
of growth was observed at concentrations of 20%, 40% and 60%, and absence of growth was observed at
80% and 100%. The minimum inhibitory concentration of conventional cleanser indicated the presence of
growth at 20% and 40% and absence of Growth at 60%, 80% and 100%. The Minimum bactericidal
Concentration (MBC) taken on Propionibacteriumacnes using both Citrus limon juice and cleanser all showed
absence of growth at all the concentrations used (20%, 40%, 60%, 80% and 100%). From the research
conducted it was observed that lemon juice have strong anti Acne vulgaris effect morethan the convensional
cleansers used for the treatment of Acne vulgaris.
Mormodica charantia Linn. A Potential Antibiotic and Anti-Fungal Druginventionjournals
Resistant or multi resistant strains are continuously appearing in the treatment of ailments and this necessitates the synthesis of new drugs especially from naturally occurring plants. Momordica charantia is a very common indigenous plant of tropical and sub-tropical regions of the world. Phytochemical screening, antimicrobial analysis were conducted using recommended procedures. Ethanol and water were used for the extraction and Ultraviolet Visible and Infrared Spectroscope were used to identify the functional groups. Alkaloids, flavonoids, saponins, tannins, anthraquinones were all present in both the ethanolic and aqueous extracts, terpenoids was only present in aqueous extract while cardiac glycosides was not present in both extracts. Concentration (200mg/ml) revealed the highest clear zone of inhibition in both aqueous and ethanol extracts. The zone of inhibition increases with increase in concentration of sample. The ethanol extract shows the higher clear zone of inhibition when compared to aqueous extract with a diameter of zones of inhibition of 28, 26, 24, 26, 26 and 24mm for Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginesa, Salmonella typhi and Klebsiella pneumoniae respectively. The aqueous extract has no effect at concentration (6.25mg/ml) because no clear zone of inhibition was measured. The antimicrobial activity for aqueous extract was lower than and not as effective as ethanol extract because ethanol is a phenolic compound and non-polar solvent when compared with water that is a polar solvent. Infrared spectra showed OH stretch due to phenol was observed at 3702 cm-1 , - C - H stretch (alkane) at 2972cm-1 , C - H bending vibration (alkane) at 1381cm-1 , - C - N and – C = N at 2077cm-1 , NH stretching at 3375cm-1 . The results indicate that it contained a phenolic, amine and amide substitute. Therapeutic drugs with antibiotic and antifungal activities can be synthesized from Mormodica charantia
This is an Engg Biotechnology project based on medicinal plant i.e singapore cherry or jamaican cherry tree (scientific name Muntingia calabure ), we did in 2013 in GMIT college Davangere, karanataka, India. i have complete project detail what we did..,
Detection of Alpha-Amylase Activity from Soil Bacteriaiosrjce
Alpha-amylase is one of the industrial enzymes that hydrolyze starch molecules into polymers
composed of glucose units. The enzyme has potential application in a wide number of industrial processes such
as food, textile, paper, detergent, fermentation and pharmaceutical industries. Alpha-amylase can be produced
by microorganisms, plants or animals.
Aim: The aim of this study is to detect the activity of alpha-amylase from bacteria isolated from soil
environment.
Method: Soil samples were inoculated onto the media that are rich in nutrient that favour the growth of the
bacteria and incubated for 24 hours at 37oC after which the bacterial growth was detected in form of colonies.
In this study, bacterial species belonging to the genus Bacillus were identified through phylogenetic analysis
using 16s-ribosomal RNA sequencing for detection of the enzymatic activity. Effects of pH and temperature on
the enzymatic activity were observed using DNS activity assay method.
Results: Positive response to alpha-amylase activity by the soil bacteria was observed by the formation of clear
zone of inhibition shown by the colonies on the petri plates.
Conclusions: The optimal pH and temperature activities showed that the bacteria exhibit enzymatic activity at
mesophilic temperature and acidophilic or alkalophilic pH.
Evaluation of the Antioxidant Activities of Organic Extracts from Ammodaucus ...CrimsonAlternativemedicine
Evaluation of the Antioxidant Activities of Organic Extracts from Ammodaucus leucotrichus Coss & Dur Fruit Part Harvested from the Algerian Sahara by Imad Abdelhamid El Haci in Advances in Complementary & Alternative medicine
Aromatic and medicinal plants are a good source of natural preparations containing effective bioactive compounds which can be used for different applications. This work aims to evaluate the antioxidant activity of some organic extracts of Ammodaucus leucotrichus Coss & Dur fruit part. The whole plant was collected from the region of Beni Abbas (Bechar-Algeria).
For more open access journals in Crimson Publishers please click on link: https://crimsonpublishers.com/
For more articles in open access Complementary Medicine journals please click on link: https://crimsonpublishers.com/acam/
Evaluation of the Antioxidant Activities of Organic Extracts from Ammodaucus ...CrimsonAlternativemedicine
Aromatic and medicinal plants are a good source of natural preparations containing effective bioactive compounds which can be used for different applications. This work aims to evaluate the antioxidant activity of some organic extracts of Ammodaucus leucotrichus Coss & Dur fruit part. The whole plant was collected from the region of Beni Abbas (Bechar-Algeria). Five organic extracts were obtained and the evaluation of the antioxidant activity was performed by six conventional methods. Polar organic extracts exhibited more antioxidant power then non polar extracts. The level of phenolic compounds was moderate in all extracts. The investigation of the antioxidant activity of organic extracts from fruit part of Ammodaucus leucotrichusrevealed a moderate activity tested by six conventional methods.
For more open access journals in Crimson Publishers please click on link: https://crimsonpublishers.com/
For more articles in open access Complementary Medicine journals please click on link: https://crimsonpublishers.com/acam/
Phytochemical Screening, Assessment of Mineral Content and Total Flavonoid Co...ijtsrd
In this research work, the Dalbergia Lanceolaria L., Myanmar name Thit pagan was selected to qualify and quantify the flavonoids present in it. The stem bark of Thit pagan was collected from Minbu Township, Magway Region, Myanmar. Firstly, the preliminary phytochemical test of this selected plant was carried out which gave positive for glycoside, flavonoid, polyphenol phenolic, sugar, saponin, tannin, terpene, alkaloid, and lipophenol test. Furthermore, the mineral contents of selected sample were measured by EDXRF method. Moreover, the total flavonoids of selected sample were extracted with 95 EtOH. This extract was checked for qualitative test of flavonoids. It responds positive for Ferric Chloride test, Shinoda's test and Lead Acetate test respectively. In addition, total flavonoid content of Dalbergia Lanceolaria L. was evaluated by the aluminum chloride AlCl3 method using UV Visible spectrophotometer UV 1800, SHIMADZU, UV spectrophotometer at 510 nm. The total flavonoid content of this selected sample was determined as 41.17 0.11 mg quercetin equivalent QE per gram dry weight. Arnt Win | Aye Mon Thida Nyo | Swe Swe Mon | Sabai Phyu "Phytochemical Screening, Assessment of Mineral Content and Total Flavonoid Content of Stem Bark of Dalbergia Lanceolaria L." Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-5 , August 2019, URL: https://www.ijtsrd.com/papers/ijtsrd26776.pdfPaper URL: https://www.ijtsrd.com/chemistry/other/26776/phytochemical-screening-assessment-of-mineral-content-and-total-flavonoid-content-of-stem-bark-of-dalbergia-lanceolaria-l/arnt-win
Ajuga bracteosa L., and Otostegia limbata L., are two
plant species of family Lamiaceae. The crude methanol leaves
extracts of these two medicinal plants were examined for their
antibacterial, antifungal and antioxidant (radical scavenging)
activity using 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical
against clinically important species of bacteria and fungi were
examined. Leaves extracts of these two plants were screened
against six strains of bacteria (two were gram positive i.e.
Bacillus subtilis and Staphylococcus aureus and four were gram
negative i.e. Vibrio cholerae, Enterobacter aerogenes, Escherichia
coli and Klebsiella pneumonia) and two strains of fungi
(Aspergillus niger and Aspergillus fumigatus). Eight
concentrations (15 mg/ml, 12.5 mg/ml, 10 mg/ml, 7.5 mg/ml, 5
mg/ml, 3 mg/ml, 2 mg/ml and 1 mg/ml) were used to check the
antimicrobial activity of plant extracts. Maximum inhibitory
zone 30 mm was observed in Otostegia limbata and 25 mm in
Ajuga bracteosa at 15 mg/ml mm for antibacterial activity. Ajuga
bracteosa and Otostegia limbata gave response against both
Aspergillus niger and Aspergillus fumigatus. This study
establishes the effective ethnomedicinal use of these plants in the
treatment of various infectious diseases. There is high potential
for the exploitation of the plants for development of novel
antimicrobial agents.
Phytochemical Potential and Antimicrobial Activity of Andrographispaniculataiosrjce
The Herbal medicine today ensures safety in contrast to the synthetic preparations. Herbs the Nature’s
Physician, have been reported as an important source of medicine for years and years. Using of herbs for
curing diseases dated back to prehistory and people of all continents have this old tradition.Recently, wide
research proposals highlight the property of medico potential from phytalsources. My herb of interest is also the
above said, ofcourseAndrographispaniculata (Acanthaceae) is a medicinal plant used for the treatment of
various ailments, which has been documented in history of all civilizations. The present study is to learn the
phytochemical properties and the antimicrobial activity of the above using disc diffusion method
Pharmacognostic, antioxidant and antimicrobial activity of fractions of the l...iosrphr_editor
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The Antioxidant and Antimicrobial Effect of Lamium galeobdolon L., Alpinia officinarum L. and Peganum harmala L. #Scichallenge2017
1. The Antioxidant and Antimicrobial
Effects of Lamium galeobdolon L.,
Alpinia officinarum L. and Peganum
harmala L.
Gökcan GÖKSOY
Cansu AKAY
2. Goal
• In recent years, side effects of synthetic medicine rate is increasing remarkably. Natural treatments may be
led to solve that problem.
• Therefore in our study, we investigated the antimicrobial and antioxidant effects of Lamium galeobdolon,
Alpinia officinarum and Peganum harmala plants, whose effects have not yet been clarified and aimed to
consume the plants consciously and to shed light on the later work to be done on these plants.
3. Lamium galeobdolon
• .Lamium galeobdolon, located between pollen and nectar sources, is given to plant species that make
Lamium genus from Lamiaceae family.The species that spread in Europe, Asia and North Africa are all
herbaceous plants
Plants and Properties
• Realm: Plantae
• Chapter: Magnoliophyta
• Class: Magnoliopsida
• Team: Lamiales
• Family: Lamiaceae
• Genus: Lamium
• Kind: Lamium galeobdolon
4. Plants and Properties
Alpinia officinarum
• It is a herbaceous species of ginger that grows in East Asia.It is frequently recommended among the
public in various treatments.In this context, the efficacy of a potentiator, a gas expectorant, an appetizer, a
chest emollient, and digestive stimulants have been reported.It has also been used against fungal diseases
and has been shown to be healing for colds.
• Realm: Plantae
• Chapter: Magnoliophyta
• Class: Liliopsida
• Team: Zingiberales
• Family: Zingiberaceae
• Genus: Alpinia
• Kind: Alpinia officinarum
5. Plants and Properties
Peganum harmala
• Harmal is one of the most famous plants used in popular envy, is Peganum harmala L., known as
Africa or Syria Rue.Although the smooth multi-branched stems are spread four feet or more, the plant
is rarely tall from two meters and usually appears round and lean in the habit.
• Realm: Plantae
• Chapter: Magnoliophyta
• Class: Magnoliopsida
• Team: Rosales
• Family: Nitrariaceae
• Genus: Peganum
• Kind: Peganum harmala
6. Soxhlet Extraction
In our work every plant was beaten in the mortar (Figure 5). Then 20 grams were weighed and prepared to obtain
an extract with Soxhlet extraction method in 200 mL of methanol. Extraction was continued for 6-8 hours to obtain
each extract. Extracts were stored at 40 ° C in a water bath after methanol was weighed, then dissolved in
methanol and stored at + 4 ° C until use.
7. Escherichia coli (E.coli)
• E. coli is an opportunistic pathogen that is important
to humans. E. coli has become the most studied
model microorganism for understanding bacterial
biology in general.This bacterium has long been
regarded as a non-pathogenic, facultative
microorganism found in the normal intestinal flora
of humans and warm-blooded animals. However,
infection of the urinary system in the 1920s and
gastroenteritis in the children in the 1940s were
detected.
Antimicrobial Activity Studies
8. Mueller Hinton Agar(mha)
• Mueller-Hinton-Agar (mha) was used during the production of E. coli bacteria and
administration of the disks.
• 38 g mha was weighed in 1 liter distilled water and heated in the heater with magnetic stirrer.
• It was sterilized in an autoclave for 20 minutes at 121 ° C. At the end of the sterilization, it was poured
into sterile plastic petri dishes and frozen and it was raised in the fridge.
• Compound:
• Beef 2.0 g / L; Casein hydrolyzate 17.5 g / L; Starch 1.5 g / L; Agar 17.0 g / L.
Antimicrobial Activity Studies
9. Antimicrobial Activity Studies
•The E. coli was used for our study was obtained from Istanbul University Medical Faculty Medical
Microbiology Department.
•Suspensions were prepared with the bacteria from the fresh sterile culture with the help of a loop. The
suspension blur is set to be equivalent to the drift of the Mcfarland 0.5 standard. For this step to be true; 0.5
McFarland tube and inoculum tube were compared with the eye under sufficient light.
Preparation of Disk Diffusion Test
• The inoculum suspension was used within 15 minutes after
adjustment.
• A sterile suspension was immersed in the bacterial
suspension. The surface of the dried agar was rotated by 60
° so that there was sowing and this process was repeated
twice.
10. Discs Used for Extracts
• For the prepared extracts, Whatman No: 1 brand filter paper was used and 0.6 mm diameter disks were
prepared and sterilized. The extracts were soaked in 20μL and dried at sterilizer.
• The antimicrobial activity of the Alpinia officinarum plant is very high. Although Peganum harmala was
partially effective, Lamium galeoblon plant showed almost no effect.
Antimicrobial Activity Studies
MethanolCefotaxime
Lamium
galeoblon
Alpinia
officinarum
Peganum
harmala
11. Antimicrobial Activity Studies
Plants Disk Diffusion Zone Diameter (cm) ± SD
İşlemler Positive Control Experimental
Group
Negative Control
Lamium
galeobdolon 4,0 ± 0,152 2,1 ± 0,346 2,0 ± 0,118
Alpinia officinarum
4,2 ± 0,216 3,0 ± 0,268 2,1 ± 0,108
Peganum harmala
3,8 ± 0,186 2,6 ± 0,342 2,1 ± 0,208
• The most antimicrobial properties of our studies the plants shown
are Alpinia officinarum> Peganum harmala> Lamium galeobdolon
respectively.
12. Antioxidant Activity Studies
•Lipid peroxidation occurs when the balance between free radicals and antioxidant defense
systems of the body have changed in favor of the radical, defined as oxidative stress, which
is the destruction of the end cell membrane and the formation of tissue damage.
•Antioxidant substances inhibit cellular damage by inhibiting the formation of active
oxygen or by retaining the active oxygen that is formed, which promotes oxidation, which
stops the formation of degenerative diseases. E and C vitamins are phenolic and
carotenoids materials.These vitamins are in the forefront with antioxidant functions in
terms of human health.
13. Antioxidant Activity Studies
• In our study, we prepared solutions of methanol extracts of different concentrations ranging from 20 mg / mL to
1.25 mg / mL of extracts of Alpinia officinarum,Lamium galeobdolon, Peganum Harmala plants used in our work.
• Alpinia officinarum,Lamium galeobdolon, Peganum Harmala was divided into 3 groups.
• We was take 0.1 mL of the extract solution and shake it by adding 3.9 mL of DPPH solution (6x10-5M) prepared
with methanol.
• It was then left in the dark for 30 minutes. Absorbance values were measured 517 nm in the spectrophotometer.
14. C Vitamin (Ascorbik Acid)
• Ascorbic acid is one of the organism's most needed
substances and a powerful antioxidant source found in
fruits and vegetables, which is soluble in water and can
liberate free radicals directly.
• It’s found in the extracellular fluid of the body. Because
synthesis in the body does not take place, it needs to be
taken from the outside with food. It also has the ability
to regenerate vitamin E
17. Antioxidant Activity Results
• The concentration (IC50) value required to remove
50% of the free radicals from the media was
calculated and compared to the column graph.
Concentration Percent Inhibition
1,25 56,6
2,5 80,39
5 95,95
10 98,86
20 98,03
18. Conclusion and Discussion
• In this study, antimicrobial and antioxidant activities were revealed by using Alpinia
officinarum, Lamium galeobdolon and Peganum Harmala plants, which are used by the
public but whose effects are not yet clarified. From the graphical curves in Figure-9-10-11,
the concentration (IC50) value required to remove 50% of the free radicals from the media
was calculated and compared to the column graph.
• Alpinia officinarum appears to be the best alternative because it shows high inhibition at low
concentrations.
Antimicrobial Activity
Antioxidant Activity
• Alpinia officinarum>Lamium galeobdolon>Peganum harmala
• Alpinia officinarum> Peganum harmala> Lamium galeobdolon
19. Suggestions
• We selected plants that had not yet been clarified by our studies and revealed
information about antimicrobial and antioxidant activities.
• In this way it is thought that the people consciously consume the plants.
• It is also suggested that our work can be used as a new natural resource in the
fields of food and pharmacy supported by more advanced studies that will shed
light on the next studies.
20. References
• C.Kaur, and H. C. Kapoor, (2011). Antioxidants in fruits and vegetables-the millennium’s health. Int. J. Food Sci. Tech. 36, 703-725,.
• Colak C. (2014). Determination of Heavy Metal and Mineral Nutrient Elements in Roots and Flowers of Some Medicinal Plants Widely
Used in Conventional Treatments in Turkey. Marmara University Institute of Science and Technology. Department of Biology. Istanbul
• Deveci M. (2012). An Investigation on Plant Species Diversity in Colchic Province (Turkey), African Journal of Agricultural Research
Vol. 7(5) , 820-843.
• Ustaçelebi, Ş. (1999). Basic and Clinical Microbiology. Güneş Kitabevi. Ankara.
• Wasteson Y. (2002). Zoonotic Escherichia coli, Acta Vet Scand; 43 (Suppl 1): 79-84.
• Zargari A (1988). Medicinal plants. Tehran University Press, Iran. (2):619.