The document discusses mitochondrial dysfunction in insulin resistant mice (DKO mice) as a model for type 2 diabetes. Electron microscopy showed DKO mice had larger, fewer mitochondria than controls. Foxo1, a transcription factor regulated by insulin, was dysregulated in DKO mice, affecting genes controlling mitochondrial function. Immunoblotting showed altered levels of fission/fusion proteins in DKO mice mitochondria. Respiration and ATP production were lower in DKO mice mitochondria, as was electron transport chain activity. Ppargc1a, a regulator of mitochondrial biogenesis, was highly expressed but inhibited by acetylation in DKO mice. Restoring Ppargc1a function or deleting Foxo1 improved mitochondrial morphology and