This document describes a study conducted at Los Alamos National Laboratory to investigate the evolution of influenza A virus (IAV) under artificial selection pressure. The researchers engineered host cells to express IAV proteins and infected these "supplemented" cells with IAV over multiple passages. They hypothesized that the virus would evolve to efficiently infect the supplemented cells while losing ability to infect unmodified cells. Preliminary results found measurable viral replication in supplemented cells expressing one IAV protein (PB1C1) over generations, but limited replication in cells expressing another protein (M1), suggesting it may interfere with virion packaging. The goal is to better understand viral evolution and identify novel antiviral strategies.