Suppose you wanted to study genes controlling the structure .pdf

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Suppose you wanted to study genes controlling the structure of bacterial cell surfaces. You decide to start by isolating bacterial mutants that are resistant to infection by a bacteriophage that binds to the cell surface. The selection procedure is simple: Spread cells from a culture of sensitive bacteria on a petri plate, expose them to a high concentration of phages, and pick the bacterial colonies that grow. To set up the selection you could (1) spread cells from a single liquid culture of sensitive bacteria on many different plates and pick every resistant colony; or (2) start many different cultures, each grown from a single colony of sensitive bacteria, spread one plate from each culture, and then pick a single mutant from each plate. Which method would ensure that you are isolating many independent mutations.

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(1)Through antibiotic resistance, bacteria will resist the action of antibiotic and there are different ways through which bacteria will become antibiotic resistance. Conjugation: Here the resistant bacteria will transfer genetic material responsible for antibiotic resistance to another bacterium by forming direct contact with them through sex pilus. Transduction: The resistance traits of one bacterium will get packed into the head portion of the virus (bacteriophage) and the release phage progeny from these antibiotic resistant bacteria will infect susceptible bacteria and transfer the antibiotic resistance genes to them. Transformation: Here bacteria capable of undergoing transformation will take up short fragments of naked DNA responsible for antibiotic resistance from the surrounding environment by direct uptake. By undergoing genetic mutation: By undergoing genetic mutations, the bacteria will produce chemicals which will eliminate the action of antibiotics and in some cases the target will be modified to escape from antibiotic action. (2) The genetic code is universal and bacterial cell will try to translate the gene to produce the protein, but the proteins will not similar to human proteins because Bacteria are lacking splicing mechanism it cannot splice out the introns from exons and thus it will try to transcribe and translate the gene with both introns and exons giving different proteins. In addition to this the proteins produced by bacteria will be different form human cells because, bacteria used 70S ribosomes and the translation it does is different from the human ribosome and another thing is codon usage is different in bacteria and we will have different genomic tRNA pool and the post-translation modification present in human cells is missing in bacteria. (3) Not all bacteria present in environment can be culturable, what you can see through microscope will far outweigh the number of colonies that grow on a Petri plate and this shows that you cannot culture all the bacteria. Through sophisticated microscopic techniques you can identify the unculturable bacteria and through molecular techniques, such as metagenomic sequencing will help you to get more details about this. Solution (1)Through antibiotic resistance, bacteria will resist the action of antibiotic and there are different ways through which bacteria will become antibiotic resistance. Conjugation: Here the resistant bacteria will transfer genetic material responsible for antibiotic resistance to another bacterium by forming direct contact with them through sex pilus. Transduction: The resistance traits of one bacterium will get packed into the head portion of the virus (bacteriophage) and the release phage progeny from these antibiotic resistant bacteria will infect susceptible bacteria and transfer the antibiotic resistance genes to them. Transformation: Here bacteria capable of undergoing transformation will take up short fragments of naked DNA responsible for antibio.

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A bacteriophage population is introduced to a bacterial colony that is in stationary phase with rapidly depleting nutrients. Appraise the following scenarios and select which one would favor long-term survival of the bacteriophage. A. The bacteriophages infect the remaining viable cells and integrate their genetic material into the host cell genome thereby preserving their genome. B. The bacteriophages do not infect the bacterial cells since they are dying; they remain inert outside of the cells. C. The bacteriophages infect the bacterial cells and go through all stages of development apart from release, thus keeping the progeny phage from entering an inhospitable environment. Solution Answer : C. The bacteriophages infect the bacterial cells and go through all stages of development apart from release, thus keeping the progeny phage from entering an inhospitable environment..

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1. Suppose you wanted to study genes controlling the structure of bacterial cell surfaces. You decide to start by isolating bacterial mutants that are resistant to infection by a bacteriophage that binds to the cell surface. The selection procedure is simple: Spread cells from a culture of sensitive bacteria on a petri plate, expose them to a high concentration of phages, and pick the bacterial colonies that grow. To set up the selection you could (1) spread cells from a single liquid culture of sensitive bacteria on many different plates and pick every resistant colony; or (2) start many different cultures, each grown from a single colony of sensitive bacteria, spread one plate from each culture, and then pick a single mutant from each plate. Which method would ensure that you are isolating many independent mutations

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