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5. As know, a virulent strain ofstaphylococcus aureus has arisen that is resistant to most you
likely antibiotics. The strain (methicillin-resistant S aureus or MRSA) is commonly found in
hospitals.
Solution
b) Function-driven approach - because in this methods sequecning of the microbial DNA from
the soil sample is not required (as in sequence-driven approach) and detects only those genes
having specifc function like antibiotic activity by allowing the expression of the gene in a
compatable host cell like the E.coli using different screening assays. This method allows the
detection of completely new genes having new and known functions.
1) Isolation of microbial cells from the soil sample.
2) Extraction and purification of total DNA contant.
3) Breaking down of purified DNA into fragments of different size.
4) DNA fragments are then cloned into a cloning vector and transformed into host cells (E.coli)
resulting in a metagenomic library.
5) The transformed cells are then grown in a culture plate containing MRSA and incubated
overnight.
6) Next day the culture plate are observed for inhibition zone. If present then the particular
transformed cell are selected and grown seperately.
7) Check for the type of antibiotics.

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5. As know, a virulent strain ofstaphylococcus aureus has arisen that.pdf

  • 1. 5. As know, a virulent strain ofstaphylococcus aureus has arisen that is resistant to most you likely antibiotics. The strain (methicillin-resistant S aureus or MRSA) is commonly found in hospitals. Solution b) Function-driven approach - because in this methods sequecning of the microbial DNA from the soil sample is not required (as in sequence-driven approach) and detects only those genes having specifc function like antibiotic activity by allowing the expression of the gene in a compatable host cell like the E.coli using different screening assays. This method allows the detection of completely new genes having new and known functions. 1) Isolation of microbial cells from the soil sample. 2) Extraction and purification of total DNA contant. 3) Breaking down of purified DNA into fragments of different size. 4) DNA fragments are then cloned into a cloning vector and transformed into host cells (E.coli) resulting in a metagenomic library. 5) The transformed cells are then grown in a culture plate containing MRSA and incubated overnight. 6) Next day the culture plate are observed for inhibition zone. If present then the particular transformed cell are selected and grown seperately. 7) Check for the type of antibiotics.