- The document describes a study that examined the gene expression profile in mouse mesenteric lymph nodes in response to treatment with three known food allergens (peanut agglutinin, ovalbumin, beta-lactoglobulin). - Microarray analysis identified differentially expressed genes in response to each allergen treatment, and real-time RT-PCR was used to validate candidate biomarker genes. - The results suggest that differentially expressed genes in response to known food allergens may serve as candidate biomarker genes for assessing potential allergenicity of genetically modified foods.

1. Mouse Mesenteric Lymph Node Transcriptome Profile And Allergic Reactions In Response To Common Food Allergens Treatment Mainul Husain University of Guelph, Guelph, Ontario, Canada

11. Overlapping And Unique Genes from 3 Treatment Groups BLG Treatment Group (1361 Genes) OVA Treatment Groups (533 Genes) PNA Treatment Groups (488 Genes) 92 131 77 988 175 169

12. Gene Ontology (GO) Analysis 204 Significant (P<0.05) GO Biological Processes Enriched with the Genes from BLG Treatment Group 327 Significant (P<0.05) GO Biological Processes Enriched with the Genes from OVA Treatment Groups 296 Significant (P<0.05) GO Biological Processes Enriched with the Genes from PNA Treatment Groups 24 30 112 104 139 114

13. Gene Ontology (GO) Biological Processes GO Biological Processes GO ID BLG Treatment PNA Treatment OVA Treatment Genes P-Value Genes P-Value Genes P-Value Response to stimulus 50896 100 0.005 50 0.002 50 0.011 Muscle development 7517 16 0.033 9 0.021 10 0.014 Cytokine and chemokine mediated signaling pathway 19221 10 0.000 5 0.006 4 0.038 Acute inflammatory response 2526 9 0.001 5 0.004 6 0.001 Humoral immune response 6959 8 0.001 5 0.002 6 0.001

15. T-Cell Receptor Signaling Pathway

16. IL-7 Signal Transduction Pathway