Appetence maturation and control in Anopheles gambiae

Dany Arsic, PhD Appetence in the mosquito Anopheles gambiae : maturation and nutritional control © CDC

Anopheles gambiae factsheet ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Gonotrophic discordance ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Appetence internal needs attractive stimuli suitable conditions adapted behavioral response Appetence is the willingness of a mosquito submitted to the right needs + stimuli + conditions to produce the adapted behavioral response of searching for a host (or plant nectar) Willingness assumes physiological readiness ! host-seeking biting nectar feeding

(co-)factors on appetence ,[object Object],[object Object],[object Object]

Part 1 Maturation of appetence ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Part 1 Part 2 Part 3

[object Object],[object Object],[object Object],[object Object],Jones & Gubbins 1978 females males virgin females inseminated females Part 1 Part 2 Part 3

Behavioral assay ,[object Object],28°C = ambient TT 37°C Part 1 Part 2 Part 3 I R

Behavioral assay ,[object Object],Part 1 Part 2 Part 3

Conclusions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Part 1 Part 2 Part 3

Part 2 Physiological and behavioral consequences of feeding ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Part 1 Part 2 Part 3

CDC/ Dr. William Collins real-time concentration of the blood meal meal: ca. 6 μ l content: 1-2 μ l

Aim ,[object Object],[object Object],[object Object],[object Object],Part 1 Part 2 Part 3

Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Part 1 Part 2 Part 3

[object Object],[object Object],Part 1 Part 2 Part 3

Results feeding behavior ,[object Object],Part 1 Part 2 Part 3 single meal, full engorgement repeated meals feeding behavior midgut crop diet destination heated (optimum 36°C) pH buffered to 7.4 membrane to feed through room temperature pH = 8 (no buffering) direct contact with soaked filter paper diet condition needed for feeding all other diets 10% sucrose diet

Results post-meal biting (1) ,[object Object],Part 1 Part 2 Part 3

Results post-meal biting (2) ,[object Object],appetent females injection of 1 μ l NaCl 0.9% micro-syringe inserted but no injection still appetent (22/25) not attracted (29/38) control test Part 1 Part 2 Part 3

Results follicle development ,[object Object],[object Object],[object Object],[object Object],24h after the meal final size at 48h (blood): 500 μ m Part 1 Part 2 Part 3

Conclusions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Part 1 Part 2 Part 3

Part 3 Nutritional signaling ,[object Object],[object Object],[object Object],[object Object],Part 1 Part 2 Part 3

signals elicited by a blood meal ovarian ecdysteroidogenic hormone (OEH) yolk protein precursors (eg. Vitellogenin) Part 1 Part 2 Part 3 modified from Attardo et al. (2005) insulin-like peptides

TOR / p70 S6K pathway response to nutrients and growth factors fat body +P Part 1 Part 2 Part 3 modified from Attardo et al. (2005)

Experiments ,[object Object],[object Object],[object Object],[object Object],[object Object],Part 1 Part 2 Part 3

Results functional genomics ,[object Object],[object Object],Part 1 Part 2 Part 3 s6k ilp3 inR

Results immunoblots ,[object Object],[object Object],[object Object],1: blood 2: albumin+AAs 3: saline 4: unfed Part 1 Part 2 Part 3

General discussion ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Thanks to… ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Appetence maturation and control in Anopheles gambiae

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Appetence maturation and control in Anopheles gambiae