Mycotoxin screening is now extremely easy and rapid thanks to Tecna Smart Strips. These lateral flow tests enable the qualitative and quantitative detection of aflatoxin B1, deoxynivalenol, zearalenone and fumonisins in cereals and feed within 5 to 10 minutes. Thus they are the perfect tool for incoming goods check. Most of the kits share a common sample preparation, which, thanks also to the dedicated reader enable an easy parallel screening of multiple mycotoxins in the same sample. Visit our website at www.tecnalab.com or contact us at export@tecnalab.com to learn more about our tests for mycotoxins.

1. The rapid and cost effective
screening solution for multiple
mycotoxins screening

2. Mycotoxins co-occurrence
Almost 70% of the
cereals in the world
present mycotoxin
contamination
In 30% of the cases
the contamination
levels are well
above the law limits
With all these premises, mycotoxins co-occurrence is a widely diffused
phenomenon
Different toxic fungi
proliferation can be
favoured by similar
climate conditions
° C 15 20 25 30 35
ZON, T2
DON
DON
FUMONISINS
AFLATOXINS
Aspergillus flavus
Aspergillus parasiticus
Fusarium moniliforme
Fusarium verticilloides
Fusarium proliferatum
Fusarium graminearum
Mycotoxins are widely diffused toxic metabolites of different fungi
species

3. Mycotoxins toxicity main
targets are:
- Liver
- Kidneys
- Immune system
- Nervous system
- Endocryne system
- Mucosa
- Reprpductive system
The effects of mycotoxins contamination on human and animal health can
be predictable in case of single mycotoxin occurrence. However, if there
are several occurring mycotoxins, the relative synergistics effects are barely
known and can be unpredictable.
Mycotoxins co-occurrence
Noteworthy, in case of multiple mycotoxins contamination, adverse effects
might occur even if the single mycotoxins levels are lower than regulatory
ones.

4. The
field
The
harvest
The
stocking
The
transit
The
acceptance
check
The
processing
There are some critical points
when SPEED and EASE of USE of
screening tests are fundamental.
Possibility of parallel screening is
the key factor to rapidly
determine mycotoxin co-
occurrence and to asses
products safety.
Mycotoxins not only develop in
the field; they proliferate along
the whole agro-food chain as
well.
Screening along the agro-food chain
SPEED and EASE of USE of a test
not necessarily enable to avoid
the risks of mycotoxins co-
occurrence.

5. Deoxynivalenol
Aflatoxins
Zearalenone
Fumonisins
Single screening
needs
- 4 exctracts to prepare
- 4 reading sessions
- 4 time consumables usage
Parallel screening
needs
- Single exctract to prepare
- One reading session
- Use consumables just once
Parallel screening provides unravelled time and money
save on mycotoxins screening.
Tecna Smart Strips for mycotoxins enable parallel
screening for the main mycotoxins as aflatoxins,
deoxynivalenol, zearalenone and fumonisins.
Parallel screening not only provides resources save but
also reduces error probability as less steps are performed.
Single screening vs parallel screening

6. Tecna Smart Strip kits share the same sample preparation and assay
procedure:
1. Weight the ground cereal
2. Add the extracting solution
3. Shake 3 minute
4. Filter
5. Dilute
6. Mark the cassette
7. Add the extract on the strip
8. Wait 5 or 10 minutes –
incubation at ROOM TEMPERATURE
9. Read
Tecna Smart Strip - general features

7. Tecna Smart Strip – steps are illustrated in
the Smart Chart
Every kit contains an illustrated Smart Chart to support analysts in performing the assay

8. The LAB LFD READER allows to analyze up to 5 strips per time, even for
different analytes. The provided result is either qualitative or
quantitative.
Key features of the LAB LFD reader are:
• little space required
• PC connession through USB wire
• read out of max 5 strips per time – different tests also
• software for results analysis
• software to archive results
Tecna LAB FLD READER for digital
analysis of results

9. MAIZE
WHEAT
Sample
preparation
DURUM WHEAT
1:3 in 70% methanol extraction, shake for 3 minutes, filter or centrifuge, dilute 1:5 in water
extraction, shake
for 3', centrifuge,
dilute
WHEAT
Tecna Smart Strip - technical features
2 – 75 ppb
Up to 10 – 375 ppb
Detection
range
50 – 1000 ppb
Up to 100 – 2000 ppb
150 – 4000 ppb
Up to 750 – 20000 ppb
LOQ 5 ppb 75 ppb 250 ppb 500 ppb
Assay time 10 min 10 min5 min
10 ppb 100 ppb
Assay time 5 min
250 -12500 ppb
Applicability
Cut off
Quantitative analysis
Qualitative analysis

10. Aflatoxin B1 and zearalenone Smart Strips allow a qualitative pre-reading of
the result within specific cut offs.
Quantitative results are obtained for aflatoxin B1, zearalenone, fumonisins
and deoxynivalenol.
Batch specific barcode is the easy way to set the LAB LFD READER for the
ananlysis for one or more mycotoxins simultaneosly.
I.e: - analysis for one mycotoxin = 1 barcode
- analysis for three mycotoxins = 3 barcodes
Tecna Smart Strip - technical features
The plastic house of the Smart Strip can be used for labelling thereof, thus
favouring sample tracking during multiple screening for mycotoxins.

11. Smart Strip AFLA B1 20 det. – cod. MA820
Smart Strip ZON 20 det. – cod. MZ800
Smart Strip FUMO 20 det. – cod. MF800
Smart Strip DON 20 det. – cod. MD800
For more information on the rapid test line dedicated to mycotoxins
screening
export@tecnalab.com
www.tecnalab.com
For further information on mycotoxins look for the Tecna Mycotoxins Cards in all
ELISA and lateral flow kits or directly book your own deck. Tecna Mycotoxins
Cards: learning about mycotoxins has never been that easy!
Tecna Smart Strip - the product lineup