Development of a quantitative lateral-flow
immunoassay for the detection of
deoxynivalenol in cereals
About deoxynivalenol
2
•Fusarium spp. (mainly F. graminearum and F. culmorum)
WHO
•15 – 30 °C, water activity > 0.85
WHEN
...
Screening techniques
3
Fastness–Easeofuse–suitabilityfor
acceptancecontrols
Sensitivity–Characterizationofanayltes–Skill
r...
Lateral flow immunoassay
4
Lateral flow immunoassays are tests based on a kind of immunochromatography. All
reagents are g...
Why?
5
 Lateral flow devices are hence characterized by fastness and ease of
use.
 They don’t need many dedicated labora...
The test kit
Kit insert
Dilution buffer
Pouches containing the strips
Barcode Businesscard
6
Product presentation
 Assay principle: competitive lateral flow immunoassay for the
quantitative detection of deoxynivale...
Sample preparation
Weight 5 g of
ground sample
and add deionized
or distilled water
Shake thoroughly for
3 minutes
Allow s...
Assay implementation
9
Pipet the diluted
extract in the
sample well
Wait for 10 minutes
Put the developed cassettes in the...
The reader
10
LAB LFD READER
• Multiplex laboratory reader
• Ideal for testing more samples together
• Ideal for using dif...
11
 The quantification depends on the ratio of the colour intensity of the
TEST LINE (T) and the CONTROL LINE (C).
 The ...
Remarks: why SMART STRIP DON
12
# Plastic cassettes provide high manageability of the devices. A lot of room is available ...
Appendix
13
 Other analytical methods can be chosen to have different
performances
 ELISAs are now available in differen...
Contacts
14
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Smart strip don: a new, fast, reliable lateral flow for vomitoxin

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For acceptance controls, for low throughput analysis, for outdoor, on-field screening, Smart Strip DON is Tecna NEW, quick, reliable lateral flow device for the qualitative and quantitative detection of deoxynivalenol (vomitoxin) in cereals. Easy to manage, easy and fast to perform, adaptable to different needs, Smart Strip DON is the right tool to analyse such mycotoxin! For further information visit Tecna website www.tecnalab.com or contact us at export@tecnalab.com!

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Smart strip don: a new, fast, reliable lateral flow for vomitoxin

  1. 1. Development of a quantitative lateral-flow immunoassay for the detection of deoxynivalenol in cereals
  2. 2. About deoxynivalenol 2 •Fusarium spp. (mainly F. graminearum and F. culmorum) WHO •15 – 30 °C, water activity > 0.85 WHEN •Cereals (mainly wheat, durum wheat, barley, maize and their derivatives) WHERE •Human gastroenteritis with nausea, diarrhea and vomiting. •The toxicity in animals is reflected by feed refusal and growth retardation. •All animal species are susceptible (pigs > mice > rats > poultry ≈ ruminants). WHAT
  3. 3. Screening techniques 3 Fastness–Easeofuse–suitabilityfor acceptancecontrols Sensitivity–Characterizationofanayltes–Skill requirement
  4. 4. Lateral flow immunoassay 4 Lateral flow immunoassays are tests based on a kind of immunochromatography. All reagents are generally inside the device and this makes the test suitable to be performed by almost everyone, everywhere. As the sample is loaded on the sample pad, it migrates toward the conjugate pad, where it dissolves the tracer, an anti-deoxynivalenol antibody colloidal gold conjugate. Thanks to capillarity, the liquid flow migrates through the membrane, reaching the immunoreactive lines. On the Test (T) line a conjugate is absorbed. This is a competitor to the free deoxynivalenol molecules eventually present in the sample. The other line is the Control (C) line, where anti-species secondary antibodies are adsorbed and which represent the positive control of the assay. In case the control line would not be coloured, at the end of the assay time, the test is to be considered invalid.
  5. 5. Why? 5  Lateral flow devices are hence characterized by fastness and ease of use.  They don’t need many dedicated laboratory equipments.  They are a good option for factories, plants and small industies with low throughput analysis. is now avaliable on the market for the QUANTITATIVE detection of deoxynivalenol (vomitoxin) in
  6. 6. The test kit Kit insert Dilution buffer Pouches containing the strips Barcode Businesscard 6
  7. 7. Product presentation  Assay principle: competitive lateral flow immunoassay for the quantitative detection of deoxynivalenol  Applications: cereals  Measuring range: 500 - 12500 ppb  Assay time: 10 minutes  Sample preparation: water extraction, centrifugation, dilution 7
  8. 8. Sample preparation Weight 5 g of ground sample and add deionized or distilled water Shake thoroughly for 3 minutes Allow sample to settle down Centrifuge the upper phase 1 minute Dilute with the provided dilution buffer 8
  9. 9. Assay implementation 9 Pipet the diluted extract in the sample well Wait for 10 minutes Put the developed cassettes in the reader
  10. 10. The reader 10 LAB LFD READER • Multiplex laboratory reader • Ideal for testing more samples together • Ideal for using different assays per time • Up to 5 different assays can be read at a time
  11. 11. 11  The quantification depends on the ratio of the colour intensity of the TEST LINE (T) and the CONTROL LINE (C).  The T/C value is interpolated by the reader onto a MASTER- calibration curve that is uploaded with a barcode businesscard for every kit batch.  The Control line must be developed and homogeneous, otherwise the test is invalid  Both readers are able to verify wether the assay is compliant or not Results achievement Validation  Specificity 100%: no compliant samples are detected as positive  Sensitivity 100%: all > 500 ppb samples are detected as positive.
  12. 12. Remarks: why SMART STRIP DON 12 # Plastic cassettes provide high manageability of the devices. A lot of room is available to write the name or the code of each sample, thus ensuring its traceability. # The sample preparation is dramatically easy: no solvent, incubator, vortex is necessary. # There are no reagents to prepare and mix, no strip to cut or dry: the sample extract is ready to be dropped on the sample pad. The reaction is incubated 10 minutes at room temperature. # The LAB LFD READER allows to save time when more samples are run together
  13. 13. Appendix 13  Other analytical methods can be chosen to have different performances  ELISAs are now available in different formats 1. Master-curve calibrated, the most cost effective layout, ideal for those implementing a few samples 2. Fast, single replicate layout: 15 – 20 minutes assays 3. Double replicates, to have higher accuracy, reliability and precision
  14. 14. Contacts 14

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