2. Bacteria:
Bacteria is a single-celled (unicellular)
microorganism that does not have a nucleus or
any other membrane-bound organelles.
3. Purpose of culturing:
Culturing is a method of multiplying microbial
organisms by letting them reproduce in
predetermined culture media under controlled
laboratory conditions. Microbial cultures are
foundational and basic diagnostic methods used
extensively s a research tool in molecular
biology.
4. Objectives:
To become familiar with the necessary nutrients
and environmental factors for culturing of
microorganisms in laboratory
To understand the decontamination or
sterilization process using an autoclave
To learn the procedure used in preparing media
needs for culturing of microorganisms
5. Continued..
Isolation of bacteria
Maintenance of pure stock culture and standard
culture
Enumeration of bacteria in samples
Increasing the number of bacteria so as we get
them in visible form as colonies
6. Principles:
Bacteria are cultured in sterilized, nutritionally
rich liquid or solid media
The liquid media Broth is taken in test tube and
Solid media Agar is contained in Petri dish to form
Agar plate
Cultivation of bacteria needs some material which
is suspected to contain bacteria
On solid Agar plate Bacteria grows as colonies.
Each colony growing form a single bacteria
