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)
Optical
density
at
Optical
density
at
Optical
density
at
Ratio
(260/280
)
Concentr
ation of
DNA
(𝝁𝒈/𝒎𝒍)260nm 280nm 230nm
1.315 1.136 1.673 1.157 6575
.
.
QUANTIFY DNA CONCENTRATION SOLUTION BY SPECTROPHOTOMETER
QUANTIFY DNA CONCENTRATION SOLUTION BY SPECTROPHOTOMETER
QUANTIFY DNA CONCENTRATION SOLUTION BY SPECTROPHOTOMETER
QUANTIFY DNA CONCENTRATION SOLUTION BY SPECTROPHOTOMETER
QUANTIFY DNA CONCENTRATION SOLUTION BY SPECTROPHOTOMETER
QUANTIFY DNA CONCENTRATION SOLUTION BY SPECTROPHOTOMETER
QUANTIFY DNA CONCENTRATION SOLUTION BY SPECTROPHOTOMETER

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QUANTIFY DNA CONCENTRATION SOLUTION BY SPECTROPHOTOMETER

Editor's Notes

  1. With this method additional information is gained concerning the quality and purity of the extracted sample obtained.
  2. A wide range of UV spectrophotometers are available, varying from traditional instruments that quantify DNA in plates or cuvettes, to instruments such as Nanodrop (Thermo-scientific) that are designed to quantify DNA from micro-volumes of sample